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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 57, Issue 4 - Dec 2014
Volume 57, Issue 3 - Sep 2014
Volume 57, Issue 2 - Jun 2014
Volume 57, Issue 1 - Mar 2014
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Antioxidant and Inflammatory Mediators Regulation Effects of the Roots of Opuntia humifusa
Kim, Ye Jin ; Park, Chan Ik ; Kim, Soo Jin ; Ahn, Eun Mi ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 1~5
DOI : 10.3839/jabc.2014.001
The roots of Opuntia humifusa (OHR) were extracted with 80% aqueous MeOH and the concentrated extract was partitioned with EtOAc, n-butanol and
, successively. The fractions were tested using DPPH and ABST radical scavenging method. The all fractions showed potent scavenging effects. The scavenging effect of the EtOAc fraction was higher than the other fractions, with
values as DPPH;
. And, we investigated anti-inflammatory activities by examining the effects of the OHR fractions on pro-inflammatory cytokine release in the human mast cells (HMC-1). Treatment with OHR fractions clearly reduced the release of the proinflammatory cytokines tumor necrosis factor-alpha (TNF-
), interleukin (IL)-
, interleukin (IL)-6 and interleukin (IL)-8) in PMACI-stimulated HMC-1 cells. The results showed the potential of OHR as an excellent antioxidant substance and inhibiting inflammatory mediators. Therefore, OHR may be used as a therapeutic approach to various inflammatory diseases.
Effect of Soybean Curd Residue Fermented by Monascus pilosus on the High fat Diet-Induced Obese Mice
Lee, Sang-Il ; Lee, Ye-Kyung ; Kim, Soon-Dong ; Lee, In-Ae ; Choi, Jongkeun ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 7~15
DOI : 10.3839/jabc.2014.002
This study investigated anti-obesity and antioxidant effects of dietary non-fermented soybean crud residue (SCR) and fermented SCR by Monascus pilosus (FSCR) in high-fat induced-obese mice. SCR and FSCR were supplemented with high-fat diet at 2% (wt/wt) dose for 8 weeks. Both SCR and FSCR significantly lowered body weight, epididymal fat weight and weight gain rate compared to high-fat diet control (HC) group and FSCR group showed lowest weight gain rate. In addition, it was observed that serum and hepatic lipid profiles including triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol were significantly improved by supplementing SCR or FSCR. Furthermore, SCR and FSCR administration showed increase of glutathione content and decrease of hepatic lipid peroxide content, serum aminotransferase activity, and hepatic xanthine oxidase activity. On the other hand, activities of reactive oxygen species scavenging enzyme such as superoxide dismutase, glutathione S-transferase and glutathione peroxidase in two test groups were higher than those of HC. Lastly, in comparison with SCR, FSCR was more effective in restoring obesity-related biomarkers to normal level in high-diet induced obese mice. In conclusion, the present study indicates that FSCR could have not only anti-obese effects such as inhibition of abdominal fat accumulation, but also protective effects of cardiovascular disease such as atherosclerosis by decreasing serum and hepatic lipid contents. Furthermore, these results suggest that experimental diets in this study could alleviate hepatic damage caused by overproduction of reactive oxygen spices (ROS) due to obesity via inhibition of ROS generating activities and induction of ROS scavenging activities.
Mechanism for Antioxidant Activity of Nardostachys chinensis root Extract
Heo, Jee-In ; Kim, Jeong-Hyeon ; Lee, Jeong-Min ; Kim, Sung Chan ; Park, Jae-Bong ; Kim, Jaebong ; Lee, Jae-Yong ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 17~22
DOI : 10.3839/jabc.2014.003
Nardostachys chinensis (N. chinensis) has been used in traditional medicine as a sedative and analgesic. It has been reported that N. chinensis extract has an antioxidant activity. However, the mechanism has not been elucidated. In this study, we showed that FOXO3a was activated by N. chinensis extract. FOXO3a is a transcriptional factor that involved in cell cycle arrest, DNA repair, apoptosis, and detoxification of reactive oxygen spices (ROS). Protein level of FOXO3a was increased by N. chinensis extract whereas phospho-FOXO3a (Thr 32) was not changed. Promoter activities of target genes of FOXO3a such as MnSOD, p27, and GADD45 were increased by N. chinensis extract. Among target genes, protein level of MnSOD was increased by N. chinensis extract, and this leads to removal of ROS level in human embryonic fibroblast (HEF) cells. These results suggested that N. chinensis extract has an antioxidant activity by upregulation of MnSOD through FOXO3a activation.
Preparation and Quality Characteristics of Mul-kimchi Added with Chlorella
Kim, Dong Chung ; Won, Sun Im ; In, Man-Jin ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 23~28
DOI : 10.3839/jabc.2014.004
Mul-kimchi was prepared with addition of 0.025, 0.05, and 0.1% (w/v) chlorella powder and fermented at
for 6 days. Quality characteristics of the Mul-kimchi were evaluated in terms of acid production (pH and titratable acidity) and lactic acid bacterial counts during fermentation. The addition of chlorella powder stimulated the growth of lactic acid bacteria and significantly enhanced the acid production. After 3 days fermentation, titratable acidity of chlorella Mul-kimchi was 0.12-0.14% and was higher than that (0.11%) of Mul-kimchi made without chlorella. The acid production and the number of viable lactic acid bacterial cell increased with increasing the concentration of added chlorella powder. The sensory score of Mul-kimchi added with 0.05% chlorella powder showed the highest values in taste and overall acceptability among the tested Mul-kimchi preparations. When chlorella Mul-kimchi preparations incubated for 3 days were kept at
for 19 days, their quality characteristics were well maintained through storage period. According to sensory score and storage ability, the optimum concentration of chlorella powder was around 0.05%.
Extraction and Stabilization of Anthocyanin Pigments from Morus alba Fruits
Seo, Kyeong-Hwa ; Lee, Dae-Young ; Jeong, Rak-Hun ; Kim, Young-Eon ; Kim, Young-Rae ; Hong, Eock-Kee ; Bang, Myun-Ho ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 29~32
DOI : 10.3839/jabc.2014.005
This study was initiated to search for the most effective method for extraction of anthocyanins from Morus alba Fruits using organic acids and to evaluate the stability of the pigments at various storage environments. The anthocyanins were effectively extracted by addition of organic acids such as citric acid, malic acid and fumaric acid. The anthocyanins were not degraded at
and under a fluorescent light with 183 lux, but sharply degraded at
The Interaction of Polysaccharides Isolated from Auricularia Polytricha with Human Serum Albumin
Wang, Wei ; Zhang, Guoguang ; Zou, Jinmei ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 33~40
DOI : 10.3839/jabc.2014.006
Polysaccharides have attracted great attention for their wide range of applications in biological and medical fields. In this paper, the interaction of polysaccharides with human serum albumin (HSA) was systematically investigated by fluorescence (FL) spectroscopy and circular dichroism (CD) spectra under different conditions. The Stern-Volmer quenching constants (
) at different ionic strength and pH were calculated, and information of the structural features of HSA was discussed. FL and CD results indicate that both hydrophobic and electrostatic interactions play important roles during the binding process. The quenching of the fluorescence resulting the binding of polysaccharides and HSA is static.
Antiulcerogenic and Anticancer Activities of Korean Red Ginseng Extracts Bio-transformed by Paecilomyces tenuipes
Kim, Young-Man ; Choi, Won-Sik ; Kim, Hye Jin ; Lee, Eun-Woo ; Park, Byeoung-Soo ; Lee, Hoi-Seon ; Yum, Jong Hwa ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 41~45
DOI : 10.3839/jabc.2014.007
In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.
Antioxidant and Cytotoxic Activity of Compounds from the Stem of Juncus effusus
Kim, Ye Jin ; Park, Chan Ik ; Park, Jae Sung ; Ahn, Eun Mi ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 47~51
DOI : 10.3839/jabc.2014.008
The stems of Juncus effusus were extracted with 70% aqueous ethanol and the concentrated extract was partitioned with ethyl acetate, n-butanol and
, successively. Two compounds were isolated from the ethyl acetate fraction through the repeated silica gel and Sephadex LH-20 column chromatographies. According to the results of physico-chemical and spectroscopic data including NMR and IR, the chemical structures of the compounds were determined as dehydroeffusol (1) and effusol (2). Dehydroeffusol and effusol exhibited potent scavenging activity for DPPH and ABTS radicals with the
in DPPH assay, and as
in ABTS assay, respectively. The compounds also significantly inhibited the proliferation of human cancer cell lines, AGS and A549.
Xanthine and Aldehyde Oxidase Inhibitory Activities, and Antihyperuricemic Effects of Fermented Smilax china L. Leaf Extracts and Fractions
Lee, Sang-Il ; Lee, Ye-Kyung ; Kim, Soon-Dong ; Cheng, Jinhua ; Yang, Seung Hwan ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 53~59
DOI : 10.3839/jabc.2014.009
To evaluate the inhibitory effect of xanthine oxidase (XO) and aldehyde oxidase (AO), and antihyperuricemic effect by Aspergillus oryzae fermented Smilax china L. leaf extracts and fractions, we observed extracted yield by each solvent, the content of total polyphenol and total flavonoid (TF), the activities of XO and AO, and serum uric acid level. Extracted yield (g/kg) by 80% ethanol (EtOH) was 13.56, those of n-hexane, dichloromethane (DICM), ethylacetate (EtOAc) and n-butanol fraction (BuOH) were 1.35-3.33. Furthermore, total polyphenol content (mg/g-extract) of EtOAc fraction, BuOH fraction, DICM fraction and EtOH fraction is 478.07-501.26, 259.49-289.02, 165.03-232.27, 134.02-196.54, respectively. Those of fermented EtOAc and DICM fraction was 4.85 and 40.74% higher than that of non-fermented fraction, respectively, while the other fermented fractions were lower than those of non-fermented fractions. And total flavonoid content (mg/g-extract) of EtOAc fraction was higher than those of other fractions. Additionally, TF of fermented EtOAc and BuOH fraction is 10.56 and 60.17% higher, than that of fermented fraction, respectively, although those of the other fermented fractions was lower than that of non-fermented fractions. On the other hand, XO inhibitory activities of all fermented fractions was significantly higher than that of all non-fermented fraction, while those of fermented EtOAc (75.02%) and BuOH fraction (65.59%) was markedly higher than that of non-fermented fraction (39.42 and 5.34%), respectively. In addition, AO inhibitory activities of DICM and EtOAc fraction was 81.82 and 77.93% higher, respectively, than those of the other fractions, and those of fermented fractions as with XO were significantly higher than that of non-fermented fractions. Meanwhile, serum uric acid level (SU) of hyperuricemic control mice (HC, 6.98 mg/dL) was 1.83 folds higher than that of normal control (NC, 3.82 mg/dL). Furthermore, SU in the group treated with EtOAc fraction decreased in a dose dependent manner compared with the allopurinol control group, although those of fermented fractions were significantly lower than those of non-fermented fractions. This study suggests that fermented Smilax china L. leaf extracts may regulate the XO and AO inhibitory activities and antihyperuricemic effect due to aglycone components from glycoside form flavonoids by fermentation of A. oryzae.
Fractionated Aged Black Garlic Extracts Enhance Growth of Anti-My-10 Hybridoma Cells and Production of IgG1 Antibody
Lee, Ji Young ; Chung, Namhyun ; Lee, Yong Kwon ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 61~63
DOI : 10.3839/jabc.2014.010
Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth-promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.
Characterization of Burcucumber Biochar and its Potential as an Adsorbent for Veterinary Antibiotics in Water
Lim, Jung Eun ; Kim, Hae Won ; Jeong, Se Hee ; Lee, Sang Soo ; Yang, Jae E ; Kim, Kye Hoon ; Ok, Yong Sik ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 65~72
DOI : 10.3839/jabc.2014.011
Biochar (BC) from biomass pyrolysis is a carbonaceous material that has been used to remove various contaminants in the environment. The eliminatory action for burcucumber (Sicyos angulatus L.) as an invasive plant is being consistently carried out because of its harmfulness and ecosystem disturbance. In this study, burcucumber biomass was converted into BCs at different pyrolysis temperatures of 300 and
under a limited oxygen condition. Produced BCs were characterized and investigated to ensure its efficiency on antibiotics` removal in water. The adsorption experiment was performed using two different types of antibiotics, tetracycline (TC) and sulfamethazine (SMZ). For the BC pyrolyzed at a high temperature (
), the values of pH, electrical conductivity, and the contents of ash and carbon increased whereas the yield, mobile matter, molar ratios of H/C and O/C, and functional groups decreased. Results showed that the efficiency of BCs on antibiotics` removal increased as pyrolysis temperature increased from 300 to
(38 to 99% for TC and 6 to 35% for SMZ). The reaction of
EDA (electron-donor-acceptor) might be involved in antibiotics` adsorption to BCs. BC has potential to be a superior antibiotics` adsorbent with environmental benefit by recycling of waste/invasive biomass.
Risk Assessment of Agricultural Worker`s Exposure to Fungicide Thiophanate-methyl during Treatment in Green Pepper, Cucumber and Apple Fields
Choi, Hoon ; Kim, Jeong-Han ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 73~81
DOI : 10.3839/jabc.2014.012
The present study was carried out to assess exposure and risk to thiophanate-methyl wettable powder for agricultural worker during mixing/loading and application with power sprayer in green pepper, cucumber and apple fields. Dermal exposure was measured with patches, gloves, socks and masks, while inhalation exposure was evaluated with personal air pump and solid sorbent. Those methods were full validated before experiment. During mixing/loading, dermal exposure amount in green pepper, cucumber and apple fields was
, corresponding to mean 0.007, 0.001 and 0.005% of prepared active ingredient, respectively. The major exposed part for mixer/loader was hands (78-92%). Dermal exposure amount for applicator in green pepper, cucumber and apple fields was
, corresponding to mean 0.024, 0.016 and 0.013% of applied active ingredient, respectively. The main body parts of exposure in apple field were hands, while thighs and shins in other fields. Inhalation exposure amount in green pepper, cucumber and apple fields was
during mixing/loading and
for applicator, respectively. These results were suggested that main factors affecting dermal exposure were contact frequency to the plants, foliage density, hygienic behavior, work type, and working environment, while inhalation exposure was affected mainly by working environment, especially wind. In risk assessment, margin of safety for thiophanate-methyl in all cases was over 1. However, during application in green pepper field, margin of safety was close to 1.
Effects of Combined Treatment of Aqueous Chlorine Dioxide and Fumaric Acid on the Microbial Growth in Fresh-cut Paprika (Capsicum annuum L.)
Jung, Seung-Hun ; Park, Seung-Jong ; Chun, Ho-Hyun ; Song, Kyung Bin ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 83~87
DOI : 10.3839/jabc.2014.013
The effects of combined treatment of aqueous chlorine dioxide (
) and fumaric acid on the microbial growth in fresh-cut paprika were investigated. After the combined treatment, the populations of total aerobic bacteria and inoculated Listeria monocytogenes in the paprika sample were reduced by 0.82 and 1.21 log CFU/g, respectively, compared to those of the control. In addition, after 10 d of storage at
, the populations were decreased by 1.21 and 2.10 log CFU/g, respectively. The predictive model for the populations of total aerobic bacteria and L. monocytogenes in the paprika was applied during storage. The prediction equation using Gompertz model was appropriate, based on the statistical analysis such as accuracy factor and bias factor. These results suggest that the combined treatment of aqueous
and fumaric acid can be an effective technology for microbial decontamination and it can improve microbial safety by decreasing maximum growth rate and increasing lag time of bacteria in the fresh-cut paprika.
Simultaneous Determination of Benzoic Acid, Caffeic Acid and Chlorogenic Acid in Seeds of Eriobotrya japonica and their Antibacterial Effect
Jeong, Jun-Mo ; Lee, Kyoung-In ; Kim, Sun-Min ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 89~93
DOI : 10.3839/jabc.2014.014
We aim to develop a simple method for simultaneous and quantitative determination of benzoic acid, caffeic acid and chlorogenic acid in seeds of Eriobotrya japonica. In addition, antibacterial effect of these three phenolic acids was examined. A basic method is performed on the high performance liquid chromatography system coupled to an UV-detector (230 nm) and reverse phase C-18 column (
). Each phenolic acid was confirmed via liquid chromatography-mass spectrometry (MS)/MS system under the multiple-reaction monitoring with negative-ion electrospray ionization (ESI(-)) mode. It is demonstrated that the method was could be applied to samples for an analytical study of the phenolic acids. On the other hand, three phenolic acids in seeds of E. japonica exhibited antibacterial effect against several pathogenic bacteria. Of these, benzoic acid was found to have stronger antibacterial effect.
Reduction of Agricultural Non-point Pollution Source by Scenarios of Best Management Practices on Cropping System Alternatives of Main Upland Crop in Saemangeum Watershed
Son, Jae Gwon ; Lee, Gyeong Ae ; Yoo, Dong Su ; Cho, JaeYoung ;
Journal of Applied Biological Chemistry, volume 57, issue 1, 2014, Pages 95~101
DOI : 10.3839/jabc.2014.015
Nonpoint pollution sources from agricultural activities are a major cause of water quality impairment. A nutrient management program utilizes farm practices that maintain efficient crop production systems and control agricultural nonpoint pollution sources. The objectives of present study were to identify appropriate best management practices (BMPs) according to changes of cropping system of main upland crop for reducing AGNPs loadings and to simulate the effects of the application of the several BMPs scenarios in Saemangeum watershed. The selected BMP scenarios were: 1) to convert naked barley and hulled barley to hairy vetch or chinese milk vetch, 2) to convert red pepper to soybean crop, and 3) to combine two scenarios, converting naked barley and hulled barley to hairy vetch or chinese milk vetch + converting red pepper to soybean crop. As a result of BMPs application, the crop requirement of nitrogen and phosphorus for upland crop reduced nitrogen by 41% and phosphorus by 47% in scenario 1, whereas scenario 2 reduced nitrogen by 30% and phosphorus by 23%. Overall, scenario 3 reduced nitrogen by 72% and phosphorus by 70% in agricultural non-point pollution sources associated with chemical fertilizer and livestock manure in Saemangeum watershed.