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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 57, Issue 4 - Dec 2014
Volume 57, Issue 3 - Sep 2014
Volume 57, Issue 2 - Jun 2014
Volume 57, Issue 1 - Mar 2014
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A1E Induces Apoptosis via Targeting HPV E6/E7 Oncogenes and Intrinsic Pathways in Cervical Cancer Cells
Ham, Sun Young ; Bak, Ye Sol ; Kwon, Tae Ho ; Kang, Jeong Woo ; Choi, Kang Duk ; Han, Tae Young ; Han, Il Young ; Yang, Young ; Jung, Seung Hyun ; Yoon, Do Young ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 103~111
DOI : 10.3839/jabc.2014.016
A1E is an extract from traditional Asian medicinal plants that has therapeutic activities against cancers, metabolic disease, and other intractable conditions. However, its mechanism of action on cervical cancer has not been studied. In order to ascertain if A1E would have pronounced anti-cervical cancer effect, cervical cancer cells were incubated with A1E and apoptosis was detected by nuclear morphological changes, annexin V-FITC/PI staining, cell cycle analysis, western blotting, Reverse-transcription polymerase chain reaction, and measurement of mitochondrial membrane potential. Expression of human papiloma virus E6 and E7 oncogenes was down-regulated in A1E-treated cervical cancer cells, while p53 and retinoblastoma protein levels were enhanced. A1E also perturbed cell cycle progression at sub-G1 and altered cell cycle regulatory factors in SiHa cervical cancer cells. A1E activated apoptotic intrinsic pathway markers such as caspase-9, caspase-3 and poly ADP-ribose polymerase, and down-regulated expression of Bcl-2 and Bcl-xl. A1E induced mitochondrial membrane potential collapse and cytochrome c release, and inhibited phosphatidylinositol 3-kinase (PI3K)/Akt, key factors involved in cell survival signaling. Taken all these results, A1E induced apoptosis via activation of the intrinsic pathway and inhibition of the PI3K/Akt survival-signaling pathway in SiHa cervical cancer cells. In conclusion, A1E exerts anti-proliferative action growth inhibition on cervical cancer cells through apoptosis which demonstrates its anti-cervical cancer properties.
A5E promotes Cell growth Arrest and Apoptosis in Non Small Cell Lung Cancer
Bak, Ye Sol ; Ham, Sun Young ; O, Baatartsogt ; Jung, Seung Hyun ; Choi, Kang Duk ; Han, Tae Young ; Han, Il Young ; Yoon, Do-Young ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 113~122
DOI : 10.3839/jabc.2014.017
A5E is complex of several medicinal herb ethanol extracts. The aim of this study is investigating the anticancer effect for non-small cell lung cancer. The antitumor effects of A5E on NCI-H460 were examined by regulation of cell proliferation, apoptosis, cell cycle arrest, mitochondrial membrane potential (
), and apoptosis-related protein. Cell proliferation was measured by MTS assay. Apoptosis induced by A5E was confirmed by Annexin V-fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) staining, and cell cycle arrest was measured by PI staining. NF-
translocation was detected by immunofluorescence and MMP (
) was measured by JC-1 staining. The expression of extrinsic pathway molecules such as FasL and FADD were elevated, and procaspase-8 was processed by A5E. In addition, intrinsic pathway related molecules were altered. The Bcl-2 and Bcl-xl levels decreased, Bax increased, and cytochrome C was released. In addition, the mitochondrial membrane potential collapsed, and caspase-3 and poly-(ADP-ribose) polymerase were processed by A5E. Moreover, A5E affected the cellular survival pathway involving phosphatidylinositol 3-kinase (PI3K)/Akt and NF-
. PI3K and Akt were downregulated, also NF-
expression was decreased, and nuclear translocalization was inhibited by A5E. These results suggested that A5E delays proliferation, inhibit cell cycle progression and induce apoptosis in human lung cancer cell. We conclude that A5E is a potential anticancer agent for human lung carcinoma.
Antioxidant and Antimicrobial Activities of Camellia Oleifera Seed Oils
Zhou, Qing-Fen ; Jia, Xue-Jing ; Li, Qian-Qian ; Yang, Rui-Wu ; Zhang, Li ; Zhou, Yong-Hong ; Ding, Chun-Bang ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 123~129
DOI : 10.3839/jabc.2014.018
The antioxidant and antimicrobial activities of Camellia oleifera seed oil were studied. Four kinds of seed oil samples were prepared, crude oil and refined oil, extracted by cold pressing method (CPC, CPR), and organic solvent extraction (OSC, OSR). Antioxidant activity analysis was measured in 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, ferric reducing Ability of Plasma, and 2,2-diphenyl-1-picrylhydrazyl assays. Besides, the percentage of inhibition of red blood cells hemolysis induced by 2,2`-azobis(2-amidnopropane) dihydrochlorid, the lag time of LDL conjugated dienes formation in vitro, and the inhibitors of loss in tryptophan fluorescence were all used to estimate the antioxidant activity of the samples. The total phenolic contents (TPC) were detemined by Folin-Ciocalteu method. The TPC of the C. oleifera seed oils can be arranged in descending order: CPC (
) > OSC (
) > CPR (
) > OSR (
). And the oils were investigated for activity against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The results showed the antioxidant activity of crude oil by cold pressing method was stronger than others, and all oils did inhibit activity of the top three bacteria expert A. niger. The further significance of the study contributes to measure the antioxidant and antimicrobial activity of the potential health benefits by the different methods of preparation and the oil of C. oleifera seeds acting as free radical scavenger, pharmaceuticals and preservatives may offer some information in medicine and cosmetic not just in food field.
Exploration of Metallic Contamination in Fish Species of the Polluted Rivers in Bangladesh
Rahman, Mokhlesur ; Jiku, Abu Sayem ; Alim, Abdul ; Kim, Jang-Eok ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 131~136
DOI : 10.3839/jabc.2014.019
An attempt was made to assess metal ionic toxicity levels of different fishes in the polluted rivers viz., Buriganga and Turag. Fish samples collected from two polluted rivers were analyzed for the levels of metals such as Cd, Cr, Cu, Mn, Pb, and Zn in order to elucidate the status of these contaminants in fish meant for human consumption. The detected concentrations of Cr, Cu, Mn, and Zn ions in fish species collected from the polluted rivers were below the toxic levels and did not appear to pose a threat. Among the analyzed metals, Cd and Pb ions were detected above the permissible levels in liver and muscle tissues of stinging catfish (Heteropneustesfossilis), spotted snakehead (Channapunctata) and wallago (Wallagoattu) collected from the polluted rivers causing toxicity for human consumption. Stinging catfish (Heteropneustesfossilis) was the species found to highly bioaccumulate these metals. Fish species bioconcentrated appreciable amounts of Cd and Pb as toxic metals in the liver as compared to the muscle. Levels of these toxic metals varied depending on different tissues in fish species.
Molecular Cloning and Characterization of a Gene Encoding Thermostable Pectinase from Thermotoga maritima
Kim, Chung Ho ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 137~140
DOI : 10.3839/jabc.2014.020
A gene encoding thermostable pectinase (TmPec) was isolated from hyperthermophilic microorganism, Thermotoga maritima. The open reading frame (ORF) of TmPec gene is 1,104 bp long and encodes 367 amino acid residues with a molecular weight of 40,605 Da. To analyze the enzymatic activity and biochemical properties, the ORF of TmPec gene excluding putative signal sequence of 27 amino acids was introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. Protein concentration of purified recombinant TmPec was 1.1 mg/mL with specific activity of 56 U/mg protein on pectin. The recombinant TmPec showed the highest activity at around
, and at around pH 6.5. It was stable at temperature below
. In the presence of
, the activity of recombinant TmPec was increased to 146.3% of normal level. In contrast,
and Mn2+ showed strong inhibition to the recombinant TmPec.
Application of Electron Beam Irradiation for Insect Pest Control in Agricultural Products Quarantine [Case study: Myzus persicae and Planococcus citri (Risso)]
Lee, Gyeong-Ae ; Park, Min-Goo ; Cho, Jae-Young ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 141~144
DOI : 10.3839/jabc.2014.021
The effects of electron beam irradiation on control of Myzus persicae and Planocococcus citri (Risso) were evaluated with the changes of mortality, emergence rate, fecundity, and egg hatchability. As a result of this study, M. persicae and P. citri (Risso) was not directly affected by electron beam irradiation with low doses (30, 60, 90, and 120 Gy) but the irradiation had effects on inhibition of development and reproduction of the pests.
Enhanced Anti-oxidant Activity Effects of Smilax china L. Rhizome Water Extracts Added with Its Fermented Leaf Water Extracts
Lee, Sang-Il ; Lee, Ye-Kyung ; Kim, Soon-Dong ; Shim, Soon-Mi ; Yang, Seung Hwan ; Cheng, Jinhua ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 145~152
DOI : 10.3839/jabc.2014.022
To evaluate the improving effects of antioxidant activity, we observed antioxidant capacities such as electron donating ability (EDA), Ferric reducing antioxidant power (FRAP), inhibitory activity of xanthine oxidase (XO) and aldehyde oxidase (AO), and sensory characteristics on mixture of Smilax china L. root water extract added with water extract of fermented S. china L. leaf by Aspergillus oryzae (FSCL). Those contents of mixture with higher ratio of FSCL were proportionally high. And OD475 of mixture with higher ratio of FSCL was almost proportionally high ($R^2
Effects of Inoculation of Rhizomicrobial Strains on Plant Growth at the Early Germination Stage
Yoo, Jae Hong ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 153~157
DOI : 10.3839/jabc.2014.023
Plant-growth-promoting rhizobacteria can affect plant growth by various direct and indirect mechanisms. This study was conducted to determine the ability of some rhizobacterial strains to enhance the seed germination of Lactuca sativa (lettuce) and Raphanus sativus (radish). Seeds were inoculated using a spore suspension (
) and incubated in a growth chamber at
under dark conditions and 65% RH. Azotobacter chroococcum and LAP mix inoculation increased the plumule length of L. sativa by 1.3, 0.8, and 0.7 cm, respectively, in comparison to the uninoculated control. Pseudomonas putida showed an increase of only 0.6 cm in plumule length when compared to the control. Inoculation of A. chroococcum, P. putida, and LAP mix enhanced the seed germination rate of R. sativus, by 10, 5, and 30%, respectively, in comparison with the uninoculated seeds. The results demonstrated that the inoculation of seeds by select rhizobacterial strains showed remarkable enhancement to the radicle length of lettuce and radish seedlings.
Extraction and Purification of an Antifungal Antibiotic Saccharide from Bacillus sp.
Yoo, Jae Hong ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 159~160
DOI : 10.3839/jabc.2014.024
An antifungal antibiotic was extracted three times using n-butanol from the culture broth of Bacillus sp. Bioassayguided column chromatography with silica gel and Sephadex LH-20 yielded 62 mg of the original active compound from 1 L of culture broth. The minimal inhibitory concentration values were 25 and
against Pyricularia oryzae and Pellicularia filamentosa, respectively. Based on results obtained from the analysis of the structure of the antibiotic using MS, NMR, and IR spectroscopy, the antifungal antibiotic was shown to consist of only six of fructose.
Pre-freezing Treatment of Blueberry, Korean Raspberry, and Mulberry
Park, Seung-Jong ; Jung, Seung-Hun ; Park, Jong-Tae ; Kim, Ha-Yun ; Song, Kyung Bin ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 161~164
DOI : 10.3839/jabc.2014.025
To establish the pre-freezing treatment and to secure microbial safety of blueberry, Korean raspberry, and mulberry, the effects of chemical sanitizers on the populations of microorganisms in the berries were examined. Among the treatments, the combined treatment of 50 ppm aqueous chlorine dioxide and 0.1% fumaric acid reduced most the populations of total aerobic bacteria in the blueberry, Korean raspberry, and mulberry by 2.56, 2.26, and 2.56 log CFU/g, respectively, compared to the control. The populations of yeast and mold in the blueberry, Korean raspberry, and mulberry by the combined treatment were also reduced by 2.24, 2.08, and 1.49 log CFU/g, respectively. These results suggest that the combined treatment can be useful for reducing the microbial contamination and maintaining the quality of frozen berries.
Identification of Secondary Metabolites from the Stems of Viburnum erosum
In, Seo-Ji ; Seo, Kyeong-Hwa ; Song, Na-Young ; Song, Myoung-Chong ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 165~170
DOI : 10.3839/jabc.2014.026
The stems of Viburnum erosum were extracted with 80% MeOH. The concentrated extract was partitioned with EtOAc, n-BuOH, and
. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated
, octadecyl silica gel, and Sephadex LH-20 column chromatographies. Based on NMR, MS, and IR spectroscopic data, the chemical structures were determined as betulinic aldehyde (1), koaburside (2), (6R,7E,9R)-9-hydroxymegastigma-4,7-dien-3-one-9-O-
-D-glucopyranoside (3), and byzantionoside B (4). All the compounds were isolated for the first time from the stems of Viburnum erosum.
Application of L-Arginine as a Substitute for Inorganic Polyphosphate in Pork Sausage Production
Kim, Yong-Duk ; Lee, Jun-Soo ; Park, Joon-Hyun ; Park, Dong-Cheol ; Jeon, Young-Seung ; In, Man-Jin ; Oh, Nam-Soon ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 171~174
DOI : 10.3839/jabc.2014.027
-Arginine was applied to processed pork sausages and evaluated as a substitute for commonly used inorganic polyphosphate. Processed pork sausage was prepared with an addition of 0.25, 0.5, and 1.0% (w/w)
-arginine. Quality characteristics of the processed pork sausages were than evaluated in terms of pH, moisture content, emulsion stability and textural properties. The pH of sausage increased with increasing
-arginine concentration. Moisture content of 0.25%
-arginine sausage, emulsion stability of 0.5%
-arginine sausage, and textural properties of 1.0%
-arginine sausage were similar to those of 0.5% polyphosphate sausage, respectively. In terms of sensory evaluation, taste and overall acceptability of 0.5%
-arginine sausage were slightly higher than those of 0.5% polyphosphate sausage and textural properties were reversed. According to sensory score and quality characteristics,
-arginine had a potential as a substitute for inorganic polyphosphate and the optimum concentration of
-arginine was around 0.5%.
Phytosterols from the Rice (Oryza sativa) Bran
Jung, Ye-Jin ; Park, Ji-Hae ; Shrestha, Sabina ; Song, Myoung-Chong ; Cho, Suengmok ; Lee, Chang-Ho ; Han, Daeseok ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 175~178
DOI : 10.3839/jabc.2014.028
Three phytosterols of rare occurrence, schleicheol 2 (1),
-hydroxysitosterol (2), and
-hydroxysitosterol (3), were isolated from the n-hexane fraction of rice (Oryza sativa) bran, for the first time. Some nuclear magnetic resonance (NMR) assignments in the literatures are inaccurate. This study employed two-dimensional NMR experiments to identify exact peak assignments.
Methanol Extract of Alpinia katsumadai Hayata has Anti-viral Activity by Inhibition of α-glucosidase
Lee, Doseung ; Boo, Kyung Hwan ; Kim, Young Cheon ; Lee, Jin-Man ; Kim, Seong Cheol ; Lee, Wang Shik ; Riu, Key Zung ; Lee, Dong-Sun ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 179~182
DOI : 10.3839/jabc.2014.029
In the present study, we investigated the effects of methanol extracts from Alpinia katsumadai Hayata against antiviral potential underlying mechanism in glucosidase inhibition. Syncytium formation in Newcastle disease virus (NDV)-infected baby hamster kidney (BHK) cell originates from the trafficking of viral glycoprotein into cell-surface. Methanol extracts inhibited not only syncytium formation, but also trafficking of glycoprotein, hemagglutinin-neuraminidase (HN), onto cell-surface. A. katsumadai extracts showed the inhibitory activities (
-glucosidase. These results suggested that blue chanterelle extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.
Bacillus subtilis Fermentation for Enhancement of Feed Nutritive Value of Soybean Meal
Kook, Moo-Chang ; Cho, Seok-Cheol ; Hong, Young-Ho ; Park, Hoon ;
Journal of Applied Biological Chemistry, volume 57, issue 2, 2014, Pages 183~188
DOI : 10.3839/jabc.2014.030
In order to increase the nutritional quality of soybean meal (SBM) as an animal feed, Bacillus subtilis TP6, a previously isolated strain from an Indonesian traditional fermented soybean food, Tempeh, was used as a starter organism for solid-state fermentation. In the pre-treated SBM with water content of 60% (v/w), B. subtilis TP6 was grown to a maximum viable cell number of
. Compared to control, crude protein in Bacillus fermented SBM was increased by 16%, while raffinose, stachyose, and trypsin inhibitors were reduced by 31, 37, and 90%, respectively. The Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that proteins in the fermented SBM were remarkably hydrolyzed into smaller molecular masses, resulting in a decrease in large sized proteins. Our data suggested that B. subtilis fermentation could increase the nutritive value of SBM through reduction of anti-nutritive factors and improvement of protein quality by hydrolysis of soy protein. In addition, B. subtilis TP6 produced a functional ingredient, poly-
-glutamic acid which has various health benefits.