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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 57, Issue 4 - Dec 2014
Volume 57, Issue 3 - Sep 2014
Volume 57, Issue 2 - Jun 2014
Volume 57, Issue 1 - Mar 2014
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Effect of the Addition of Antimicrobial Materials Before and After Aging on the Physicochemical Properties of Low-Salt Kochujang during Storage
Seo, Young-Eun ; Bae, Hwa-Sook ; Kim, Dong-Han ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 287~294
DOI : 10.3839/jabc.2014.045
To improve the shelf life of low-salt Kochujang, Korean hot pepper paste, antimicrobial materials were added at different times before and after aging. The kochujang was then packaged and stored at
for 15 weeks, and changes in microbiological and physiochemical properties were evaluated. Hunter a- and b-values decreased considerably during storage. The total color difference (
) was greater in the ethanol-chitosan (EC) treatment than in the control and after pasteurization (A-P) treatment. Gas was produced until the seventh week of storage. The control and the A-P treatments produced more gas than the other treatments, and these had the largest number of yeasts and aerobic bacteria. The pH of the EC treatment was higher than that of the other treatments, and the A-P treatment had the highest level of titratable acidity. During storage, the oxidation-reduction potential was lower in the EC and ethanol-mustard-chitosan (EMC) treatments. The reducing sugar content decreased remarkably in the control and A-P treatments, with high production of ethanol. There was a significant change in the content of amino-type nitrogen in the control and A-P treatments, and the content of ammonia-type nitrogen was lowest in the EMC treatment. In the sensory test of kochujang, the ethanol-mustard (EM) and ethanol (E) treatments were significantly higher than the EC, control, and A-P treatments (p <0.05). EM or E alone was effective in extending the shelf life of kochujang when added before aging.
Isolation and Identification of Triterpenoids from the Mulberry (Morus alba) Root Bark
Jung, Jae-Woo ; Park, Ji-Hae ; Jung, Ye-Jin ; Lee, Chang-Ho ; Han, Daeseok ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 295~299
DOI : 10.3839/jabc.2014.046
The mulberry (Morus alba L.) root barks were extracted with 80% aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-BuOH, and
fractions. From the EtOAc fraction, five triterpenoids were isolated through the repeated silica gel and octadecyl
column chromatographies. According to the results of physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the chemical structures of the triterpenoids were respectively determined as
-acetyl amyrin (2), lupeol (3), betulinic acid (4), and glutinol (5). Compounds 1, 3, and 5 were isolated for the first time from the mulberry root bark.
Antioxidant Activity Analysis of Useful Compounds from Artemisiae Annuae Herba Using On-line Screening HPLC-ABTS
Lee, Kwang Jin ; Ma, Jin Yeul ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 301~305
DOI : 10.3839/jabc.2014.047
The Antioxidant activity screening identification of five kind compounds in Artemisiae annuae herba with the on-line screening high performance liquid chromatography (HPLC)
assay. The various experimental variables such as the extraction time (h) and extraction solvent composition (%) of dipping method were investigated efficiently extraction at the room temperature
. The results, the highest yield of total extract amount (0.458 g, 15.250%) was obtained by dipping method with 100% water and extraction time to 3 h. And the on-line screening HPLC-
assay method was rapid and efficient to search for bioactivity from natural products.
The Effects of Using Artificial Sweeteners and Coffee Grounds in Chocolate Filling on Quality Characteristics and Glycemic Index
Kang, Suna ; Lee, Jin Sook ; Jeong, Areum ; Kim, Eunha ; Park, Sunmin ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 307~312
DOI : 10.3839/jabc.2014.048
Chocolate is restricted for obese and diabetic patients due to high in sugar contents. We investigated shell-chocolate fillings with low calorie content and low glycemic indexes using alternative sweeteners such as tagatose and xylose and adding coffee grounds. Chocolate filling was made with sugar, cacao and butter in the original recipe. Sugar was substituted with tagatose or xylose and 3% coffee ground was added in each chocolate filling and substituted for 3% of the cocoa. Water retention and spreadability of the chocolate filling was significantly higher in the tagatose+coffee ground group in comparison to the sugar. In the chromaticity test, chocolate filling made with tagatose was darker in comparison to that made with sugar: brightness (L) was lower and redness (a) and yellowness (b) were higher than the sugar filling. Sensory evaluation revealed that chocolate filling made with tagatose had high score in 4 categories such as taste, smell, texture of foreign substance, overall acceptance. After consuming 60 g of the chocolate with different fillings, blood glucose levels of subjects at 1 and 2 h were significantly lower in the tagatose and tagatose+coffee ground groups than the other groups. In conclusion, using tagatose as the sweetener in chocolate filling has a beneficial effect on lowering blood glucose levels and increasing water retention, spreadability and sensory scores. Coffee ground did not have additive effect on quality of chocolate filling and blood glucose levels. These results indicate that shell chocolate made with tagatose can be used as a snack for diabetic patients.
Xylanase Production by Mixed Culture Using Crude Hemicellulose from Rice Straw Black Liquor and Peat Moss as an Inert Support
Shata, Hoda Mohamed Abdel Halim ; El-Deen, Azza Mohmed Noor ; Nawwar, Galal Abdel Moen ; Farid, Mohmed Abdel Fattah ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 313~320
DOI : 10.3839/jabc.2014.049
Black liquor (BL) is a by-product of rice straw pulping process. It is a low costs raw material for production value-adding proteins and enzymes, which has been paid more and more attention to reduce its environmental pollution. Mixed cultures of micelial fungi, Trichoderma reesei Northern Regional Research Laboratory (NRRL)11236, Trichoderma reesei NRRL 6165 and Aspergillus niger strains NRC 5A, NRC 7A, and NRC 9A were evaluated for their ability to produce xylanase using crude hemicellulose (CHC) prepared from BL and peat moss as an inert support under solid state fermentation (SSF). The most potent strains, A. niger NRC 9A (818.26 U/g CHC) and T. reesei NRRL 6165 (
U/g CHC), were used in a mixed culture to enhance xylanase production by co-culturing under SSF. In the mixed culture, xylanase production (
U/g CHC) was nearly1.3 and 10.6-fold increases over the activities attained in their monocultures, A. niger NRC 9A and T. reesei NRRL 6165, respectively. Optimization of the culture parameters of the mixed culture SSF process, concentration of ammonium sulfate and corn steep liquor, CHC/peat moss ratio, inoculum size and ratios of the two strains, initial pH value, initial moisture content and incubation time, exhibited a significant increase (
U/g CHC) in xylanase production than before optimization.
Isolation and Identification of Adenosine and Phlomuroside from the Aerial Parts of Oryza sativa L.
Jeong, Rak-Hun ; Lee, Dae-Young ; Cho, Jin-Gyeong ; Baek, Yoon-Su ; Seo, Kyeong-Hwa ; Lee, Dong-Geol ; Kang, Hee-Cheol ; Kim, Ji-Young ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 321~324
DOI : 10.3839/jabc.2014.050
Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned in n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and
fractions. From the n-BuOH fraction, two compounds were isolated through repeated silica gel and ODS column chromatography (c.c.). Based on nuclear magnetic resonance (NMR), mass spectrometry and infrared spectroscopy spectroscopic data, the compounds were identified to be adenosine (1) and phlomuroside (2). Especially, the configuration of both the anomer hydroxyl groups was determined as
from the coupling constants of the anomer protons (J =6.0 and 7.6 Hz) in the
spectra. This is the first report for the isolation of these compounds from Oryza sativa L.
Postharvest Treatment of Sweet Persimmon and Preparation of Its Dehydrated Product
Kang, Ji-Hoon ; Park, Seung-Jong ; Seong, Ki-Hyun ; Song, Kyung Bin ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 325~329
DOI : 10.3839/jabc.2014.051
To maintain the quality of sweet persimmon during storage, the samples were treated with a combination of 50 ppm
and 0.1% fumaric acid after harvest, packaged with low density polyethylene film, and stored at
for 35 days. The combined treatment reduced the populations of total aerobic bacteria, yeast and molds by 1.82 and 2.07 log CFU/g, respectively, compared to the control. During storage, hardness of all samples decreased, but total soluble solids and weight loss were not significantly different among treatments. In addition, high-quality dehydrated sweet persimmon was prepared using red algae extract as a dehydrating agent. The rehydration ratio and vitamin C content of red algae extract-treated sample were greater than those of hot-air dried sample. These results suggest that the combined treatment of
and fumaric acid can be useful for maintaining microbiological safety of sweet persimmon during storage and dehydration of sweet persimmon slices using red algae extract is an efficient drying method for the preparation of high-quality dehydrated sweet persimmon.
Runoff Characteristics of Nutrients from Agroforest Culture Field
Kim, Eun-Hyeok ; Cho, Jae-Young ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 331~336
DOI : 10.3839/jabc.2014.052
Sediment and nutrient loading caused by the forest to conversion of agricultural lands have led to the deterioration in near water ecosystem. This study was carried out to examine the effects of agroforest culture field and open field culture field on water quality and runoff loading of nutrient. The runoff loading of Tot-N and Tot-P in agroforest culture field were similar to open field culture field. The runoff loading of total suspended solids (TSS) in agroforest culture field and open field culture field were
in 2011 and
in 2012, respectively. Our investigation showed that the runoff loading of TSS from agroforest culture field decreased when soil cover and soil stabilization increased. Therefore, protect facility of soil erosion for early alteration of agricultural lands are needed to minimize the soil erosion from agroforest culture field.
Comparative Radical Scavenging and Nitric Oxide Production Capacity of Water Extracts of Acanthopanax divaricatus var, Albeofructus and Eleutherococcus divaricatus var. Chiisanensis
Kim, Dong-Min ; Jin, Bora ; Kim, Ji Yeon ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 337~340
DOI : 10.3839/jabc.2014.053
In the present study, water extracts of Acanthopanax divaricatus var, albeofructus and Eleutherococcus divaricatus var. chiisanensis were prepared and their antioxidant and anti-inflammatory effects were compared. The water extract of Acanthopanax divaricatus var, albeofructus showed significantly higher activity in total polyphenol and flavonoid contents, while there were no significant differences in ABTS/DPPH radical scavenging activities and ferric reducing antioxidant power. However, the water extract of Eleutherococcus divaricatus var. chiisanensis at the highest dose showed significantly lower capacity in nitric oxide production than that of Acanthopanax divaricatus var, albeofructus. Taken together, there were differences between Acanthopanax divaricatus var, albeofructus and Eleutherococcus divaricatus var. chiisanensis in total polyphenol/flavonoid contents and anti-inflammatory effect, however, the antioxidant effect was almost similar.
Environmental Behavior of Fenarimol, Chlorothalonil, and Ethoprophos in Agroforesty Field
Kim, Eun-Hyeok ; Cho, Ki-Young ; Cho, Jae-Young ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 341~345
DOI : 10.3839/jabc.2014.054
Fate of fenarimol, chlorothalinol, and ethoprophos sprayed to control disease and pest was studied in a agroforest culture field of Jangsu-gun, Jeollabuk-do, Korea. Concentrations of fenarimol, chlorothalinol, and ethoprophos in runoff water ranged mostly to 0.2 mg/L at the first rainfall-runoff event. And then was rapidly decreased than detection limit at 60 days after the application. The fenarimol and chlorothalonil residue in soil was dissipated to below detection limit at 30 days after the application. But ethoprophos was decreased to below detection limit at 135 days after the application. The concentrations of experimental pesticides were highly detected in agroforest culture field than in open culture field. It is assumed that experimental pesticides were strongly adsorbed by organic matter such as fulvic acid and humic acid.
Norsesquiterpenes from the Roots of White Kwao Krua (Pueraria mirifica)
Kwon, Jung-Hwa ; Cho, Jin-Gyeong ; Park, Hee-Jung ; Huh, Gyu-Won ; Bang, Myun-Ho ; Han, Min-Woo ; Oh, Chang-Hwan ; Ko, Sung-Kwon ; Cho, Soo-Yeul ; Chai, Kap-Yong ; Kim, Jin-Ho ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 347~352
DOI : 10.3839/jabc.2014.055
The roots of Pueraria mirifica were extracted with 70% aqueous ethyl alcohol and partitioned into ethyl acetate (EtOAc), n-butyl alcohol (BuOH), and
fractions, successively. From the EtOAc fraction, four norsesquiterpenes were isolated through the repeated silica gel, octadecyl silica gel and Sephadex LH-20 column chromatographies. On the basis of physicochemical and spectroscopic data including nuclear magnetic resonance (NMR), mass spectrometry, and infrared spectroscopy, the chemical structures were identified as megastigm-5-en-3,9-diol (1), linarionoside B (2), 3,5,6,9-tetrahydroxymegastigm-7-ene (3) and 3,4,9-trihydroxymegastigma-5,7-diene (4). Especially, the configuration of the anomer hydroxyl group was determined as a from the coupling constants of the anomer proton (J =8.0 Hz) in the
-NMR spectrum. These compounds were isolated for the first time from the roots of P. mirifica in this study.
Antioxidative Effect of Extracts from Different Parts of Kohlrabi
Pak, Won-Min ; Kim, Koth-Bong-Woo-Ri ; Kim, Min-Ji ; Kang, Bo-Kyeong ; Bark, Si-Woo ; Kim, Bo-Ram ; Ahn, Na-Kyung ; Choi, Yeon-Uk ; Yoon, Sung-Ryul ; Ahn, Dong-Hyun ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 353~358
DOI : 10.3839/jabc.2014.056
Antioxidant activities of ethanol and water extracts of flesh, leaves, and peels of green and purple Kohlrabi were measured by rancimat method, total phenolic compound (TPC), DPPH radical scavenging effect, chelating effect, and reducing power analysis. The highest TPC was observed in ethanol extract from green peel and water extract from purple leaf with 12.00 and 11.70 mg/g of dry sample, respectively. The ethanol extracts from purple Kohlrabi leaf and peel exhibited strong DPPH radical scavenging effects with reducing power while water extracts from purple Kohlrabi leaf and peel also showed strong DPPH radical scavenging with chelating effects. Antioxidant index of ethanol and water extracts from green and purple Kohlrabi measured by rancimat was lower than butylated hydroxytoluene. These results suggest that extracts from purple Kohlrabi leaf and peel exhibited higher antioxidant activities than those of green Kohlrabi, and can be potentially used as proper natural antioxidants.
Isolation and Identification of Antioxidant Compound from the Lythrum Salicaria L. Roots
Lee, Kyeong-Hee ; Lee, Dae-Young ; Lee, Seung-Eun ; Noh, Hyung-Jun ; Lee, Jeong-Hoon ; Choi, Jehun ; Park, Chun-Geun ; Kim, Seung-Yu ; Lee, Jun-Su ; Kim, Geum-Soog ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 359~363
DOI : 10.3839/jabc.2014.057
The roots of Lythrum salicaria L. were extracted in 80% aqueous MeOH and the concentrated extract was fractionated with EtOAc, n-BuOH, and
, successively. The repeated silicagel and octadecyl
column chromatographies of the EtOAc fractions led to isolation of an antioxidant compound and two major compounds. From the results of spectral data and the chemical characteristics including nuclear magnetic resonance, MS, and IR, the structures of compounds were determind as myricetin-3-O-
-D-glucopyranoside (1), oleanolic acid (2), betulinic acid (3). This is the first reported isolation of compounds (1, 2) from L. salicaria. Compound 1 as well as EtOAc, n-BuOH, and
solvent fractions were evaluated for 2,2-dipicryl-1-phenylhydrazyl radical scavenging activity.
Anti-Melanogenic, Anti-Wrinkle, Anti-Inflammatory and Anti-Oxidant Effects of Xylosma congesta leaf Ethanol Extract
Lee, Jae Yeon ; Ahn, Eun-Kyung ; Ko, Hye-Jin ; Cho, Young-Rak ; Ko, Woon Chul ; Jung, Yong-Hwan ; Choi, Kyung-Min ; Choi, Mi-Rae ; Oh, Joa Sub ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 365~371
DOI : 10.3839/jabc.2014.058
In the present study, we investigated the biological activities of Xylosma congesta leaf ethanol extract (XCO) using a variety of in vitro and cell culture model systems for anti-melanogenic, anti-wrinkle, anti-inflammatory and anti-oxidant activities. First, XCO markedly inhibited
-melanocyte stimulating hormone-stimulated melanin synthesis in B16F10 cells. Secondly, XCO marginally induced procollagen synthesis in CCD-986SK cells. Thirdly, XCO dose-dependently suppressed lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 cells. XCO did not affect cell viability at different concentrations used in this study, indicating that XCO-mediated inhibition of melanin, procollagen and NO synthesis is not mediated by cytotoxicity. Finally, XCO was found to exert anti-oxidant effect. Taken together, these findings demonstrate for the first time that XCO possesses anti-melanogenic, anti-wrinkle, anti-inflammatory and anti-oxidant activities, and suggest further evaluation and development of XCO as a functional supplement or cosmetic that may be useful for whitening skin, reducing wrinkles and treating inflammatory responses.
Immune-stimulatory Effects of Fomes fomentarius Extract in Murine Macrophages
Kim, Young Hoon ; Park, Eun Gyu ; Batsuren, Dulamjav ; Ganbaatar, Jamsranjav ; Nho, Chu Won ; Pan, Cheol-Ho ; Lee, Jae Kwon ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 373~377
DOI : 10.3839/jabc.2014.059
In this study, we demonstrated whether the extract of Fomes fomentarius (FFE; FF extract) could be used to stimulate macrophages (RAW 264.7 cells). All four doses of FFE (5, 10, 20, and
) had no significant cytotoxicity during the entire experimental period. FFE potently increased the production of nitric oxide (NO). Consistent with these observations, inducible NO synthase levels were increased by FFE in a dose-dependent manner. Moreover, FFE increased the production of tumor necrosis factor-
, interleukin (IL)-
, and IL-6 in the same cells. These stimulating effects of FFE were found to be caused by the stimulation of phosphorylation of
and MAP kinases (p38, ERK, and JNK). These results suggest that FFE may be used as new agents for wide application in the immune study of mushroom.
Histone Modifications and It's Relation with Functional Aspects
Kang, Han-Chul ; Kim, Jong-Bum ; Roh, Kyung Hee ; Kim, Hyun-UK ; Lee, Kyung-Ryeol ; Kim, Sun Hee ;
Journal of Applied Biological Chemistry, volume 57, issue 4, 2014, Pages 379~386
DOI : 10.3839/jabc.2014.060
Chromatin is an instructive DNA structure that can widely respond to external signals. An important change of chromatin is the modifications of histone for this regulation. There are accumulating lists of these modifications and the complexity of their action is gradually understood. It is evident that histone modifications play important roles in most biological processes that are involved in the expression or repression of DNA. The surface of nucleosomes is susceptible to multiplicity of modifications. Chromatin modifications can play either by eliminating chromatin contacts or by recruiting non-histone proteins to chromatin. Many of these regulations seem to be epigenetically inherited. Thus, histone modifications are closely correlated with many fundamental biological processes in animal, plant and microbial kingdoms. Failures of histone modification lead, in general, to defective chromosome condensation or decondensation, impeding many biological functions including development, maturation, and protection against various diseases.