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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 58, Issue 4 - Dec 2015
Volume 58, Issue 3 - Sep 2015
Volume 58, Issue 2 - Jun 2015
Volume 58, Issue 1 - Mar 2015
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Characterization of the Intact Form of Thermotoga maritima Pectinase TmPecN Expressed in Escherichia coli
Kim, Chung Ho ; Cheong, Jong-Joo ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 97~100
DOI : 10.3839/jabc.2015.016
The thermostable pectinase gene TmPec isolated from Thermotoga maritima was introduced into the NdeI site of pRSET-B vector and expressed in its intact form in Escherichia coli BL21. The overexpressed intact form of pectinase (TmPecN protein) was partially purified by heat-denaturation procedure. TmPecN showed the highest activity between 85 and
, and at approximately pH 6.5. Enzyme activity was stably maintained at temperatures below
. In the presence of
, pectinase activity of TmPecN increased to 128.4% of normal level. In contrast,
strongly inhibited TmPecN activity. We conclude that the biochemical properties of the intact form of TmPecN are comparable to those of the recombinant protein TmPec reported previously.
Controlling Tyrophagus putrescentiae Adults in LED-Equipped Y-Maze Chamber
Lee, Sang-Min ; Lee, Jeong-Bin ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 101~104
DOI : 10.3839/jabc.2015.017
To evaluate four different light-emitting diodes (LEDs) as potential attractants for Tyrophagus putrescentiae adults, attractiveness of blue (470 nm), green (520 nm), yellow (590 nm), and red (625 nm) LEDs were investigated at 20, 40, and 60 lx luminance intensity in LED-equipped Y-maze chamber and compared with the response to black light bulb (BLB), which is used in commercial traps. The BLB, the blue LED, the green LED, the yellow LED, and the red LED did not show the attractive to T. putrescentiae adults. These results suggested that four LEDs tested could not be used for environment-friendly control of T. putrescentiae adults.
Acaricidal Abilities and Chemical Composition of Forsythia suspense Fruit Oil against Storage and Pyroglyphid Mites
Lee, Hwa-won ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 105~108
DOI : 10.3839/jabc.2015.018
This research is aimed at evaluating the potential abilities of the natural acaricide of F. suspense oil against Tyrophagus putrescentiae and Dermatophagoides spp. Based on the
values, in contact bioassay, F. suspense oil (8.19, 3.28, and
) showed acaricidal effects against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively. Fumigant toxicities of F. suspense oil showed similar patterns as those observed with contact toxicities. GC/MS analysis showed the major components of F. suspense oil to be
-pinene (45.88%), myrtenol (13.86%), (+)-
-pinene (13.09%), (-)-trans-pinocarveol (7.34%), sabinene (6.64%) and pinocarvone (4.13%). These findings indicate that F. suspense oil has potential as a natural acaricide.
Low Density Lipoprotein-oxidation Inhibitory Phytochemicals from the Fruits of Rhus parviflora
Shrestha, Sabina ; Park, Ji-Hae ; Cho, Jin-Gyeong ; Lee, Dae-Young ; Kang, Ji-Hyun ; Li, Hua ; Jeong, Tae-Sook ; Kim Cho, Somi ; Lee, Dong-Sun ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 109~112
DOI : 10.3839/jabc.2015.019
Fruits of Rhus parviflora were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and
, successively. Purification of EtOAc fraction led to isolation of fifteen polyphenols of which structures were identified by spectroscopic methods including 2D-NMR. Most compounds apart from compound 10 inhibited low density lipoproteinoxidation within
. Among compounds, taxifolin (2), quercetin 3-O-
-L-rhamnopyranoside (13), agathisflavone (5) sulfuretin (4), and aureusidin (3) showed
values 0.9, 0.8, 5.8, 2.9, and
which were of highly significant in comparison positive control butylated hydroxytoluene with
. The results indicate fruits of R. parviflora as a source of antihypercholesterolemic compounds.
Silver Materials Induce Differential Cytotoxicity and Pulmonary Toxicity Based on Size and Shape
Pak, Pyo June ; Kang, Beob Hwa ; Chung, Namhyun ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 113~116
DOI : 10.3839/jabc.2015.020
Silver materials may be toxic in humans because they can enter the body and accumulate, typically in the lungs. We hypothesized that the cytotoxicity of naive silver materials is affected by their size and shape. Our in vitro assays revealed that the overall toxicity was in the following order: submicro-particles>wires>micro-particles. These results contrast with previous studies, which showed that silver wires are the most toxic among the three tested materials, possibly due to differences in cell lines. Evaluations of in vivo pulmonary toxicity revealed eryptosis in the cavity lining of the lung sections. The observed eryptosis was consistent with the in vitro results. Our results indicate that silver materialinduced cytotoxicity must be measured and compared using various methods.
Extraction Conditions for Phenolic Compounds with Antioxidant Activities from White Rose Petals
Choi, Jae Kwon ; Lee, Yoon Bok ; Lee, Kyun Hee ; Im, Hae Cheon ; Kim, Yun Bae ; Choi, Ehn Kyoung ; Joo, Seong Soo ; Jang, Su Kil ; Han, Nam Soo ; Kim, Chung Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 117~124
DOI : 10.3839/jabc.2015.021
The extract of white rose petals has an antioxidant effect and can be used to treat allergic disease. The purpose of this study was to identify optimal conditions for extracting antioxidative compounds from white rose petals with 2,2-diphenyl-1-picrylhydrazyl scavenging activities. A response surface methodology based on a central composite design was used to investigate the effects of three independent variables: ethanol concentration (
), extraction temperature (
), and extraction time (
). The estimated optimal conditions for obtaining phenolic compounds with antioxidant activities were as follows: ethanol concentration of 42% (
), extraction time of 80 min (
), and extraction temperature of
). The estimated optimal conditions for obtaining flavonoid compounds with antioxidant effects were an ethanol concentration of 41% (
), extraction time of 119 min (
), and an extraction temperature of
). Under these conditions, predicted response values for the phenolic and flavonoid contents were 243.5 mg gallic acid equivalent/g dry mass and 19.93 mg catechin equivalent (CE)/g dry mass, respectively.
Antioxidant and Anti-wrinkling Effects of Extracts from Vitex trifolia L.
Lee, Mi-Kyoung ; Kim, Dong-Hee ; Park, Tae-Soon ; Son, Jun-Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 125~129
DOI : 10.3839/jabc.2015.022
In this study, the antioxidant and anti-wrinkling effects of fraction from Vitex trifolia L. were investigated. Among the fractions, ethyl acetate fraction showed the highest antioxidant activities in 1-1-diphenyl-2-picryl-hydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoine-6-sulfonic acid) radical scavenging and elastase inhibition with 76, 89, and 74%, respectively, at a concentration of
. This fraction, at the concentration of
, inhibited 70% fibroblast cell viability and 86% the matrix metalloprotease (MMP)-1. In addition, the results from Western blot assay showed that this fraction (
) expressed the MMP-1 protein level by decreasing 50%. The findings suggest that the ethyl acetate fraction from V. trifolia has great potential as a cosmeceutical ingredient with antioxidant and anti-wrinkling effects.
Studies on LED Wavelength to Enhance Growth and Bio-active Compounds of Carrots
Kang, Suna ; Kim, Min-Jung ; Kim, Bong Soo ; Park, Sunmin ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 131~137
DOI : 10.3839/jabc.2015.023
Commercial greenhouse plant factories are highly efficient for controlling external factors such as floods, drought, insects, air pollution etc. However, they require substantial startup & maintenance investments and experimental research to optimize production. These facilities are especially useful for urban farming where high efficiency in small spaces is required. In this study, we investigated whether light emitting diode (LED) lights with mixed dominant wavelengths (650 nm : 550 nm : 445 nm=8:1:1, 650 nm : 445 nm=6:4) can increase the growth rate and bio-active compound content of carrots in comparison to that of fluorescent light (FL). LED with mixed wavelength (650 nm : 550 nm : 445 nm=8:1:1) increased the total weight and root circumference of carrots compared to FL. However,
-carotene contents were not significant in LED (650 nm : 550 nm : 445 nm=8:1:1). However, LED (650 nm : 445 nm=6:4) increased the
-carotene (FL: 7.27, LED: 10.48 mg/g
-carotene dried weight). These results suggested that using LED light at the ideal wavelength, at the antithesis color of the plant, might enhance plant growth and bio-active compound contents.
Evaluation of 3-week Repeated Dose Oral Toxicity on Amomum tsao-ko Extract in Balb/c Mice
Park, Ju-Hyeong ; Cho, Young-Rak ; Ko, Hye-Jin ; Jeong, Wonsik ; Ahn, Eun-Kyung ; Oh, Junho ; Oh, Joa Sub ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 139~143
DOI : 10.3839/jabc.2015.024
In the present study, we investigated the oral toxicity of Amomum tsao-ko Crevost et Lemaire, (Zingiberaceae) extract in Balb/c mice (BALB, n=60) for 3 weeks. Balb/c mice (10 mice/group, 6 group,
, 6 weeks) were orally administered for 21 days, with dosage of 250, 500, 1000, 2000 mg/kg/day. Ethanol extract of A. tsao-ko did not affect any significant change of mortality, clinical signs, organs and body weights. Also, there were not significantly difference from the naive group (control) in hematological and serum biochemical examination. Consequently, these findings indicate that 3-week treatment with the ethanol extract of A. tsao-ko was not any toxic effects in Balb/c mice and the no-observed adverse effect level (NOAEL) for oral toxicity was determined to be 2000 mg/kg/day under our experimental conditions.
Characterization of a Corn Fiber Protein Film Containing Green Tea Extract
Yang, Hyun-Ju ; Lee, Ji-Hyun ; Lee, Ji-Hyeon ; Song, Kyung Bin ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 145~151
DOI : 10.3839/jabc.2015.025
Corn fiber protein (CFP) was extracted from corn wet-milling by-product, corn fiber. CFP films containing various plasticizers and cross-linking agents were prepared and their mechanical properties were determined. Among the plasticizers and cross-linking agents used in this study, the CFP film containing 2 g fructose and 0.03% cinnamaldehyde had the most appropriate physical property. In addition, the CFP films containing green tea extract (GTE) were prepared by incorporating different amounts (0, 0.5, 1.0, 1.5%) of GTE into the film-forming solution. Tensile strength, film solubility, and opacity of the CFP films increased with the addition of GTE, whereas elongation and water vapor permeability of the CFP/GTE films decreased compared to those of the control. The antioxidant activity of the CFP/GTE film was determined in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. As a result, antioxidant activity of the films increased with increasing GTE concentration. Furthermore, antimicrobial activity against Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus increased with increasing GTE concentration. These results indicate that the incorporation of GTE could enhance antioxidant and antimicrobial activities of the CFP films.
Isolation and Identification of Phenolic Compounds from the Root Bark of Morus alba L.
Jung, Jae-Woo ; Park, Ji-Hae ; Seo, Kyeong-Hwa ; Baek, Yoon-Su ; Oh, Eun-Ji ; Lee, Dae-Young ; Lim, Dong-Wook ; Han, Daeseok ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 153~155
DOI : 10.3839/jabc.2015.026
The root barks of Morus alba L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and
fractions. The repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies of the EtOAc and n-BuOH fractions led to isolation of four phenolic compounds. The chemical structures of the compounds were determined as norartocarpanone (1), 2',4',7-trihydroxy-(2S)-flavanone (2), methyl
-resorcylate (3), and (Z)-oxyresveratrol-4-O-
-glucopyranoside (4). Compound 4 was isolated for the first time from the root barks of M. alba L.
Heavy Metal Stabilization in Soils using Waste Resources - A Critical Review
Lim, Jung Eun ; Moon, Deok Hyun ; Kim, Kwon-Rae ; Yang, Jae E ; Lee, Sang Soo ; Ok, Yong Sik ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 157~174
DOI : 10.3839/jabc.2015.027
Stabilization of metals in contaminated soils using various waste materials has been reported. Alkaline materials (limes, shells, industrial byproducts, etc.), phosphorous (P) containing materials (animal bones, phosphate rock, etc.), organic materials (composts, manures, biochars, etc.) and others (zerovalent iron, zeolite, etc.) were widely evaluated to ensure its effectiveness/applicability of stabilization of metals in soils. Stabilization mechanisms of those materials above were partially revealed, but the related literatures are still lacked and not sufficient for approaching to long-term stability/applicability in the field. The aims of this review are to summarize current knowledge of metal stabilization in contaminated soils using various waste materials and to suggest a direction for future field research.
Analysis of Mint Essential Oils from Jeju Island, Korea by Gas Chromatography-mass Spectrometry and Headspace-Gas Chromatography-mass Spectrometry
Hyun, Ho Bong ; Boo, Kyung Hwan ; Kang, Hye Rim ; Kim Cho, Somi ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 175~181
DOI : 10.3839/jabc.2015.028
Compositions of essential oils extracted from mint herb such as Mentha piperita, Mentha spicata, and Mentha
piperita var. citrate produced in Jeju were analyzed using gas chromatography-mass spectrometry (GC-MS) and headspace-GC-MS (HS-GC-MS). By the GC-MS analysis, 13 compounds were tentatively identified in Mentha piperita, Mentha spicata, and Mentha
piperita var. citrate, respectively. Peperitenone oxide, carvone, and linalool were detected as major compounds in Mentha piperita, in Mentha spicata, in Mentha
piperita var. citrate, respectively, based on the ratio of peak intensity in the total ion chromatogram. The greater number of compounds, including volatile alcohols and acetates were identified by HS-GC-MsS than by GC-MS in these all three essential oils. Similar patterns of composition were detected in both Mentha spicata and Mentha
piperita var. citrate by either one of GC-MS methods. However, in case of Mentha piperita,
-(-)-menthol, which was identified as the major compound by HS-GC-MS was detected in dramatically reduced quantity by GC-MS. Interestingly, we found that both linalyl acetate and linalool were identified as the dominant compounds in the essential oil of Mentha
piperita var. citrate.
Anti-wrinkle Activities Verification of Buplerum falcatum Extracts on CCD-986sk
Kim, Dong-Hee ; Park, Tae-Soon ; Son, Jun-Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 183~187
DOI : 10.3839/jabc.2015.029
The electron donating ability, elastase inhibitory, procollagen synthesis and Matrix metalloprotease-1 (MMP-1) activities were measured in order to verify the anti-wrinkle properties of extracts from Buplerum falcatum as a functional ingredient for cosmetic products. Electron donating ability and elastase inhibition activities were 80 and 52% at a dose of
of B. falcatum 70% ethanol extract. Pro-collagen synthesis was increased with the increase concentration of B. falcatum extract on CCD-986sk in addition to decrease the amount of protein of MMP-1. The results suggested that B. falcatum extract can be used to reduced electron donating ability, elastase, pro-collagen synthesis and MMP-1 activity and is a potential candidate for cosmedical materials.
Flavonoid Constituents of Acacia catechu
Hong, Seong Su ; Choi, Yun-Hyeok ; Suh, Hwa-Jin ; Kang, Min-Jung ; Shin, Jung-Hye ; Kwon, Oh-Oun ; Oh, Joa Sub ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 189~194
DOI : 10.3839/jabc.2015.030
Ten compounds were isolated from the ethanolic extract of the leaves of Acacia catechu (Fabaceae), and their structures were identified as nine flavonoids [(+)-catechin (1), (-)-epicatechin (2), (+)-afzelechin (3), (-)-epiafzelechin (4), (+)-mesquitol (5), kaempferol (6), quercetin (7), quercetin 3-methyl ether (8), and caryatin (9)] and an ellagic acid (10). The chemical structures of these compounds were identified on the basis of spectroscopic methods (mass spectrometry, 1D and 2D nuclear magnetic resonance) and comparison with literature values. This is the first time that the isolation of caryatin (9) has been reported from Acacia genus.
Erratum to: Dietary Supplementation of Mushroom Water Suppresses Fat Accumulation in High Fat Diet Induced-Obese Female Mice and Enhances Immune Cell Development in Non-Obese Mice
Bing, So Jin ; Ho, Manh Tin ; Hyeon, Koo ; Sophors, Phorl ; Park, Sanggyu ; Yun, Young Min ; Jee, Youngheun ; Cho, Moonjae ;
Journal of Applied Biological Chemistry, volume 58, issue 2, 2015, Pages 195~195
DOI : 10.3839/jabc.2015.061