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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 58, Issue 4 - Dec 2015
Volume 58, Issue 3 - Sep 2015
Volume 58, Issue 2 - Jun 2015
Volume 58, Issue 1 - Mar 2015
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Chemical Composition and Acaricidal Effects of Essential Oils Extracted from Ligustrum japonicum against Acaridae and Pyroglyphid Mites
Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 197~199
DOI : 10.3839/jabc.2015.031
The composition of the essential oil of Ligustrum japonicum leaves was determined by GC-MS analysis. The major constituents of L. japonicum leaf oil were germacrene D (40.50%),
-pinene (13.63%), (-)-
-caryophyllene (5.73%), and
-cadinene (5.47%). The acaricidal activities of L. japonicum oil were evaluated against acaridae and pyroglyphid mites. In the fumigant bioassay, the
values of L. japonicum oil were 16.48, 12.38, and
against Tyrophagus putrescentiae, Dermatophagoides farinae, and D. pteronyssinus, respectively. In the contact bioassay, the
values of L. japonicum oil were 8.02, 5.02, and
against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively.
RNA Binding Proteins and its Regulation of Gene Expression
Roh, Kyung Hee ; Kang, Han-Chul ; Kim, Jong-Bum ; Kim, Hyun-UK ; Lee, Kyung-Ryeol ; Kim, Sun Hee ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 201~208
DOI : 10.3839/jabc.2015.032
The role of RNA-binding proteins (RBPs) to regulate expression of genes seems to be very important. RBPs play important roles in RNA related bioprocess such as transcription, pre-mRNA splicing, polyadenylation, transport, localization, translation, turn over and maintenance of structure. Despite of many researches on RNA binding proteins, detailed mechanisms of these proteins have not been fully understood. It seems that many parts of RBPs remains unknown and should be characterized for the better understanding of gene expression. Recently, genetic, biochemical, and bioinformatic analysis of genomes revealed a vast array of RBPs and many parts are interesting to understand bioprocessing including gene expression.
Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605
Farid, Mohamed Abdel-Fattah Mohamed ; Kamel, Zinat ; Elsayed, Elsayed Ahmed ; El-Deen, Azza Mohamed Noor ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 209~218
DOI : 10.3839/jabc.2015.033
Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo's fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10;
, 1.0 and KCl, 0.5. The obtained results showed that the maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at agitation speed of 200 rpm and using vegetative fungal cells as inoculum. Moreover, results showed that optimization of medium composition and some cultivation parameters resulted in an increase of about 93.7% in the enzyme activity than the nonoptimized cultivation conditions after 96 h of cultivation. Additionally, maximum production and specific production rates recorded 2340 U/L/h and 102 U/L/h/g cells, respectively.
Anticancer Activities of the Methanolic Extract from Lemon Leaves in Human Breast Cancer Stem Cells
Moon, Jeong Yong ; Nguyen, Linh Thi Thao ; Hyun, Ho Bong ; Osman, Ahmed ; Cho, Minwhan ; Han, Suyeong ; Lee, Dong-Sun ; Ahn, Kwang Seok ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 219~226
DOI : 10.3839/jabc.2015.034
The anticancer activity of a methanolic extract from lemon leaves (MLL) was assessed in MCF-7-SC human breast cancer stem cells. MLL induced apoptosis in MCF-7-SC, as evidenced by increased apoptotic body formation, sub-G1 cell population, annexin V-positive cells, Bax/Bcl-2 ratio, as well as proteolytic activation of caspase-9 and caspase-3, and degradation of poly (ADP-ribose) polymerase (PARP) protein. Concomitantly, MLL induced the formation of acidic vesicular organelles, increased LC3-II accumulation, and reduced the activation of Akt, mTOR, and p70S6K, suggesting that MLL initiates an autophagic progression in MCF-7-SC via the Akt/mTOR pathway. Epithelial-mesenchymal transition (EMT), a critical step in the acquisition of the metastatic state, is an attractive target for therapeutic interventions directed against tumor metastasis. At low concentrations, MLL induced anti-metastatic effects on MCF-7-SC by inhibiting the EMT process. Exposure to MLL also led to an increase in the epithelial marker E-cadherin, but decreased protein levels of the mesenchymal markers Snail and Slug. Collectively, this study provides evidence that lemon leaves possess cytotoxicity and anti-metastatic properties. Therefore, MLL may prove to be beneficial as a medicinal plant for alternative novel anticancer drugs and nutraceutical products.
L-glutamine:D-fructose-6-phosphate Aminotransferase as a Key Protein Linked to Multidrug Resistance in E. coli KD43162
Lee, Sung-Eun ; Jung, Tae-Jeon ; Park, Byeoung-Soo ; Kim, Byung-Woo ; Lee, Eun-Woo ; Kim, Hye Jin ; Yum, Jong Hwa ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 227~232
DOI : 10.3839/jabc.2015.035
A microarray study has been employed to understand changes of gene expression in E. coli KD43162 resistant to ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, cefazolin, cefepime, aztreonam, imipenem, meropenem, gentamicin, tobramycin, ciprofloxacin, levofloxacin, moxifloxacin, fosfomycin, and trimethoprim-sulfamethoxazole except for amikacin using disk diffusion assay. Using Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and MALDI-TOF MS analyses, 36 kDa of outer membrane proteins (OMPs) was found to be deleted in the multidrug resistant E. coli KD 43162. Microarray analysis was used to determine up- and down-regulated genes in relation to multidrug resistant E. coli KD43162. Among the up-regulated genes, these genes were corresponded to express the proteins as penicillin-binding proteins (PBPs), tartronate semialdehyde reductase, ethanolamine utilization protein, shikimate kinase I, allantoinase, predicted SAM-dependent methyltransferase, L-glutamine: D-fructose-6-phosphate aminotransferase (GFAT), phospho-glucosamine mutase, predicted N-acetylmannosamine kinase, and predicted N-acetylmannosamine-6-P epimerase. Up-regulation of PBPs, one of primary target sites of antibiotics, might be responsible for the multidrug resistance in E. coli with increasing amount of target sites. Up-regulation of GFAT enzyme may be related to the up-regulation of PBPs because GFAT produces N-acetylglucosamine, a precursor of peptidoglycans. One of GFAT inhibitors, azaserine, showed a potent inhibition on the growth of E. coli KD43162. In conclusion, up-regulation of PBPs and GFATs with the loss of 36 kDa OMP refers the multidrug resistance in E. coli KD 43162.
Alkyl Glycosides from the Flowers of Magnolia obovata
Oh, Eun-Ji ; Seo, Kyeong-Hwa ; Kwon, Jung-Hwa ; Lee, Dae-Young ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 233~236
DOI : 10.3839/jabc.2015.036
The flowers of Magnolia obovata were extracted with aqueous MeOH and fractionated into EtOAc, n-BuOH, and
fractination. Three alkyl glycosides were isolated from the EtOAc fraction through repeated silica gel and ODS column chromatography. The structures were identified to be 2-methylbutan-1-ol-
-galacto-pyranoside (1), 2-methylbutan-1-ol-
-glucopyranoside (2), and 2-methylpropan-1-ol-
-glucopyranoside (3) on the basis of spectroscopic analyses such as fast atom bombardment mass spectrometry, infrared spectroscopy, 1D nuclear magnetic resonance (NMR) (
), and 2D NMR (gCOSY, gHSQC, and gHMBC). These compounds were isolated for the first time from the flower of M. obovata in this study.
Isolation and Identification of Triterpenoids and Sterols from the Flowers of Chionanthus retusus Lindl. & Paxton
Jung, Jae-Woo ; Seo, Kyeong-Hwa ; Oh, Eun-Ji ; Lee, Dae-Young ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 237~240
DOI : 10.3839/jabc.2015.037
The flowers of Chionanthus retusus Lindl. & Paxton were extracted with 80% aqueous MeOH at room temperature. The concentrated extract was partitioned as EtOAc, n-BuOH, and
fractions. From the EtOAc fraction, two triterpenoids and two sterols were isolated using the repeated silica gel (
) and octadecyl
(ODS) column chromatographies. According to the results of physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared. The chemical structures of the compounds were respectively determined as ursolic acid (1), corosolic acid (2),
-sitosterol (3), and daucosterol (4). All the compounds were isolated for the first time from the flowers of Chionanthus retusus Lindl. & Paxton.
Anti-inflammatory Activities of Taxifolin from Opuntia humifusa in Lipopolysaccharide Stimulated RAW 264.7 Murine Macrophages
Kim, Jaeyoung ; Lee, Yonghwa ; An, Hyeon-jin ; Lee, Jae-duk ; Yi, Yongsub ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 241~246
DOI : 10.3839/jabc.2015.038
This study was performed to investigate the antiinflammatory activities of taxifolin from Opuntia humifusa. A potent anti-oxidant activity was shown from the leaf extract at
and fruit extract at
by 1,1-diphenyl-2-picrylhydrazyl assay. Fraction of taxifolin from leaf extract identified using high performance liquid chromatography and gas chromatography/mass spectrometry. The results of cell viability indicated that taxifolin did not show cytotoxicity on RAW 264.7 cells at
of concentration. The result showed that taxifolin inhibited lipopolysaccharide (LPS)-induced production of Nitrite oxide. In addition, taxifolin inhibited LPS-induced tumor necrosis factor-
and interleukin-6 production by cytokine assay and cyclooxygenase-2 expression by western blot analysis, meaning taxifolin has a significant anti-inflammatory effect. Our results suggested that taxifolin from Opuntia humifusa showed anti-inflammatory activities.
Isolation, Identification, and Characterization of a Keratin-degrading Bacterium Chryseobacterium sp. P1-3
Hong, Sung-Jun ; Park, Gun-Seok ; Jung, Byung Kwon ; Khan, Abdur Rahim ; Park, Yeong-Jun ; Lee, Chang-Hyun ; Shin, Jae-Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 247~251
DOI : 10.3839/jabc.2015.039
In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of
and at a pH of 8.0.
Screening the Antibacterial Activities of Streptomyces Extracts against Phytopathogens Xanthomonas oryzae pathovar oryzae, Xanthomonas campestris pathovar vesicatoria, and Pectobacterium carotovorum pathovar carotovorum
Kim, Seung-Hwan ; Cheng, Jinhua ; Yang, Seung Hwan ; Suh, Joo-Won ; Song, Eun-Sung ; Kang, Lin-Woo ; Kim, Jeong-Gu ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 253~258
DOI : 10.3839/jabc.2015.040
Xanthomonas oryzae pv. oryzae (Xoo), X. campestris pv. vesicatoria (Xcv), and Pectobacterium carotovorum pv. carotovorum (Pcc) are the causative agents of bacterial blight in rice, bacterial spot in pepper, and bacterial soft rot in carrot and cabbage, respectively. To isolate novel microbial extracts with antimicrobial activities against these bacteria, approximately 5,300 different Streptomyces extracts were prepared and tested. Microbial cultures from various Streptomyces strains isolated from the Jeju Island, Baekam, Mankyoung river, Jiri mountain etc. in Korea were extracted into three different factions -secreted hydrophobic, secreted hydrophilic, and mycelia- using ethyl acetate, water, and methanol. Initially, 34, 29, and 10 extracts were selected as having antibacterial activities against Xoo, Xcv, and Pcc, respectively. Extracts 1169G4, 1172E9, and 1172E10 had the highest growth inhibition activities against both Xoo and Xcv, and extracts 1151H7 and 1152H7 showed the highest growth inhibition activities against Pcc.
The Efficacy of Lowering Blood Glucose Levels Using the Extracts of Fermented Bitter Melon in the Diabetic Mice
Park, Hye Seon ; Kim, Woo Kyeong ; Kim, Hyun Pyo ; Yoon, Young Geol ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 259~265
DOI : 10.3839/jabc.2015.041
Momordica charantia, commonly known as bitter melon, has interesting pharmacological activities such as anticancer, antiviral, antibacterial, anti-inflammatory, analgesic, and antioxidant. As supported by recent scientific reports on the beneficial effects of M. charantia, it is one of the most promising functional plants for diabetes today. In this study, we fermented the bitter melon with lactic acid bacteria and investigated the capability of controlling diabetic conditions by decreasing the blood glucose levels. After extracting the fermented bitter melon with hot water or ethanol, we tested several biological activities using mouse models. When we tested the efficacy of the glycemic control, the extracts of fermented bitter melon significantly lowered the blood glucose levels of the alloxan-induced diabetic mice. We also found that the lactic acid bacteria-fermented bitter melon protected liver damages from the treatment of alloxan monohydrates and maintained low levels of triglycerides and high levels of HDL cholesterol in these mouse models. These results suggest that our approach on fermenting bitter melon and the extracts of fermented bitter melon could lead to the possibility of the development of functional foods that contain the effectiveness of controlling blood glucose and lipid levels as well as preventing liver damages.
Whitening Effects of Solvent Fractions Isolated from Vitex rotundifolia
Yu, Jae-Myo ; Kim, Dong-Hee ; Son, Jun-Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 266~271
DOI : 10.3839/jabc.2015.042
Recently many effort focused to understand the mechanical insights of melanogenesis to develop the agent for hyper-pigmentation. So this study was performed to investigate the depigmentation of Vitex rotundifolia. With B16F10 mouse melanoma cell, we have seen inhibition of the tyrosinase, MITF, TRP-1, TRP-2, and melanin synthesis, which eventually were dose dependently decreased by Vitex rotundifolia. Specially, Vitex rotundifolia decreased the protein levels of tyrosinase and TRP-1. In conclusion, Vitex rotundifolia showed the whitening activity in all the experiments mentioned above and we expect that it can be used for preventing melanin synthesis.
Transformation of the Diatom Phaeodactylum tricornutum with its Endogenous (E)-4-Hydroxy-3-methylbut-2-enyl Diphosphate Reductase Gene
Shin, Bok-Kyu ; Jung, Yu-Jin ; Kim, Sang-Min ; Pan, Cheol-Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 273~279
DOI : 10.3839/jabc.2015.043
Phaeodactylum tricornutum is a model diatom that its genomic information and biological tools are well established. In this study, a gene encoding (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (PtHDR), a terminal enzyme of the methylerythritol phosphate pathway regulating chlorophyll and carotenoid biosynthesis, was isolated from P. tricornutum. The isolated gene was cloned into pPha-T1 vector containing fcpA promoter to prepare pPha-T1-HDR plasmid. As a positive control, pPha-T1-eGFP plasmid was constructed with egfp gene. Stable nuclear transformation was carried out with these plasmids by particle bombardment method and zeocin resistant colonies of P. tricornutum were selected on f/2 agar plate. In result, transformation efficiency was evaluated according to the amount of plasmid DNA coated with gold particles. Integration of introduced plasmids was confirmed with genomic DNA of each transformant by polymerase chain reaction. The eGFP fluorescence was visible in the cytoplasm, indicating that eGFP was successively expressed in P. tricornutum system. The transcript level of exogenous Pthdr gene was evaluated with the obtained transformants. The results presented here demonstrated that introduction of Pthdr gene into P. tricornutum chromosome succeeded and expression of PtHDR was enhanced under the fcpA promoter.
Human Neutrophil Elastase Inhibitory Alkaloids from Chelidonium majus L.
Kim, Jeong Yoon ; Lee, Ji Hye ; Song, Yeong Hun ; Jeong, Won Min ; Tan, Xuefei ; Uddin, Zia ; Park, Ki Hun ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 281~285
DOI : 10.3839/jabc.2015.044
Human neutrophil elastase (HNE) represents a good therapeutic target for the treatment of inflammatory diseases as well as invasion of microorganism. The methanol extract of a aerial part of Chelidonium majus L. showed high activity against the neutrophil elastase with an
. Due to its potency, subsequent bioactivity-guided fractionation of methanol extract led to six alkaloids (1-6), which were identified as dihydrosanguinarine (1), (s)-stylopine (2), arnottianamide (3), (+)-chelidonine (4), spallidamine (5), and N-trans-feruloyltyramine (6). Among of them, three alkaloids (2, 5, and 6) inhibited HNE in a dose-dependent manner with
ranging between 11.6 and
. Lineweaver-Burk and Dixon plots, and their secondary replots showed that alkaloids (2, 5, and 6) were mixed inhibitors of HNE. The analysis of
value proved that all inhibitors (2, 5, and 6) had reversible mixed type I mechanism.
Inhibitory Effects of Soyasaponins on Antigen-induced Degranulation in RBL-2H3 Cells
Yang, Seung Hwan ; Lee, Jung A ; Lee, Jae Yeon ; Ahn, Eun-Kyung ; Shin, Tai-Sun ; Tsukamoto, Chigen ; Chung, Gyuhwa ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 58, issue 3, 2015, Pages 287~290
DOI : 10.3839/jabc.2015.045
Soyasaponins are glycosylated, which gives rise to a wide diversity of structures and functions. We evaluated for inhibitory effects of 4 soyasaponins on antigen-induced degranulation in RBL-2H3 Cells. 4 soyasaponins had shown dose-dependently inhibited histamine and