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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
Journal Basic Information
Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 58, Issue 4 - Dec 2015
Volume 58, Issue 3 - Sep 2015
Volume 58, Issue 2 - Jun 2015
Volume 58, Issue 1 - Mar 2015
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Pesticide-Originated Persistent Organic Pollutants in Agricultural Waterways in Chungcheong Province, Korea
Lee, Hwa-Sung ; Jeon, Hwang-Ju ; Lee, Hoi-Seon ; Lee, Sung-Eun ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 291~294
DOI : 10.3839/jabc.2015.046
Persistent organic pollutants (POPs) including banned organochlorine pesticides, are found in Korean agricultural environments. Their translocation from agricultural environment to major crops has been reported. In this study, the POPs in 15 different agricultural waters of the Chungchung Province were monitored and all samples were prepared, followed by liquid-liquid fractionation and column chromatography prior to analysis using gas chromatography. Among pesticide-originated persistent organic pollutants,
-endosulfan was determined in the range of 0.01 to 1.13 ng/mL.
-Endosulfan was determined in the range of 0.01 to 0.55 ng/mL, and endosulfan sulfate was ranged from 0.13 to 1.13 ng/mL. The total sum of endosulfans reached the range of 0.38 to 1.18 ng/mL. Three pesticides being used currently were also found in samples as 2.03-2.05 ng/mL for triadimefon, 0.26-0.33 ng/mL for tolyfluanid, and 1.34-3.85 ng/mL for chlorpyrifos. Taken together, endosulfans were introduced in the agricultural waters and their fates need continuous monitoring.
Tyrosinase Inhibitory Activities of Safrole from Myristica fragrans Houtt.
Cho, Soo Jeong ; Kwon, Hyun Sook ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 295~301
DOI : 10.3839/jabc.2015.047
Five phenylpropanoids (1-5), a benzofuran neolignan (6), two 8-O-4'-neolignans (7-8), and five tetrahydrofuran lignans (9-13) were isolated from a methanol extract of Myristica fragrans seeds. The structures of 1-13 were determined by
-NMR spectroscopic data analyses and a comparison with the literature data. Compound 3 was isolated for the first time from this plant. All the isolated compounds were evaluated for their inhibitory activity against tyrosinase. Among them, safrole (1) showed significant inhibitions against both the monophenolase (
) and diphenolase (
) activities of tyrosinase. The kinetic analysis shows that safrole (1) is competitive inhibitors for both monophenolase and diphenolase. The apparent inhibition constant (
) for safrole (1) binding with free enzyme was determined to be 16.05 and
for monophenolase and diphenolase, respectively.
Analysis of γ-Aminobutyric Acid Content in Fermented Plant Products by HPLC/UV
Lee, Dong Gu ; Cho, Sunghun ; Lee, Jamin ; Cho, Seon Haeng ; Lee, Sanghyun ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 303~309
DOI : 10.3839/jabc.2015.048
-Aminobutyric acid (GABA) content in fermented plant products and their main plant materials (aerial part of Acanthopanax sessiliflorus, fruit of Crataegus pinnatifida, and whole plant of Morus alba) was determined by high-performance liquid chromatography. GABA was quantified using a reverse-phase column with a gradient elution program (water:acetonitrile =90:10 to 0:100 for 40 min). UV detection was conducted at 280 nm. GABA content was measured in fermented plant products (15.07 mg/g), aerial part of A. sessiliflorus (4.49 mg/g), fruit of C. pinnatifida (10.59 mg/g), and whole plant of M. alba (2.31 mg/g). The presence of GABA in fermented plant products, including A. sessiliflorus, C. pinnatifida, and M. alba is important in industrial application for health supplements.
Substitution Effect of Enzymatically Hydrolyzed Purple Sweet Potato Powder on Skim Milk in Yogurt Preparation
Kim, Dong Chung ; Won, Sun Im ; In, Man-Jin ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 311~316
DOI : 10.3839/jabc.2015.049
Yogurt was prepared with different substitution ratio [10, 30, and 50% (w/w)] of skim milk with enzymatically hydrolyzed purple sweet potato powder (EHPSPP) and fermented at
for 15 h. Fermentation characteristics and antioxidant activities of the yogurt were evaluated in terms of acid production (pH and titratable acidity) and lactic acid bacterial counts and DPPH radical scavenging activity, respectively. After 15 h fermentation, titratable acidity of EHPSPP yogurt was 0.80-0.89% and was lower than that (0.93%) of yogurt made without EHPSPP. The acid production and the number of viable lactic acid bacterial cell decreased with increasing the substitution ratio. The sensory score of EHPSPP yogurt prepared with 30% substitution ratio showed the highest values in taste and overall acceptability among the tested yogurt preparations. DPPH radical scavenging activity increased with increasing the substitution ratio in yogurt fermented for 12 h. The total phenolic content of 30% EHPSPP yogurt was 40% higher than that of skim milk yogurt. These results suggest that EHPSPP can be used as substituent of skim milk and the optimum substitution ratio is around 30%.
Biological Activities of Extracts from Cornus kousa Fruit
Lee, Eun-Ho ; Lee, Seon-Ho ; Cho, Young-Je ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 317~323
DOI : 10.3839/jabc.2015.050
The extracted phenolic compounds from Cornus kousa fruit for biological activities as functional resources were examined. The phenolic compounds which were extracted with water and 40% ethanol from Cornus kousa fruit were
, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of water and ethanol extracts were 84% and 86% at
phenolics, respectively. The 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid radical decolorization activity of water and ethanol extracts were 84 and 95% at
phenolics, respectively. Antioxidant protection factor in water and ethanol extracts at
phenolics were 1.93 and 1.82 PF, respectively. Thiobarbituric acid reactive substance were 69% in water extracts and 89% in ethanol extracts at
phenolics. The inhibition activity on xanthine oxidase in water and ethanol extracts was 34 and 60%, respectively. The inhibition activity on
-glucosidase was 29% in water extracts and 87% in ethanol extracts. The tyrosinase inhibitory activity was 19% in ethanol extracts. The collagenase inhibition activity of anti-wrinkle effect showed an excellent wrinkle improvement effect as 53% in water extracts and 77% in ethanol extracts at
phenolics. The hyaluronidase inhibition activity as antiinflammation effect of water extracts was confirmed to 34% of inhibition at
phenolic. The results can be expected extracts from Cornus kousa fruit to use as functional resource for antioxidant, antigout, inhibitor of carbohydrate degradation, antiwrinkle activity and antiinflammation activity.
Overexpression of Ice Recrystallization Inhibition Protein (HvIRIP) from Barley Enhances Cold Tolerance in Transgenic rapeseed plants
Roh, Kyung Hee ; Park, Jong-Sug ; Kang, Han-Chul ; Kim, Jong-Bum ; Jang, Young-Suk ; Kim, Kwang-Soo ; Yi, Hankuil ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 325~332
DOI : 10.3839/jabc.2015.051
Rapeseed (Brassica napus) is now the second largest oilseed crop after soybean. Cold temperature tolerance is an important agronomic trait in winter rapeseed that determines the plant's ability to control below freezing temperatures. To improve cold tolerance of rapeseed plants, an expression vector containing an Barley Ice recrystallization inhibition protein (HvIRIP) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into rapeseed plants. Transgenic expression of HvIRIP was proved by southern- and northern-blot analyses. The level of freezing tolerance of transgenic
plants was found to be significantly greater than that of wild-type rapeseed plants by freezing assay. Proline accumulation during cold stress was also highly induced in the transgenic rapeseed plants. The transgenic plants exhibited considerable tolerance against oxidative damage induced by cold stress. Our results indicated that heterologous HvIRIP expression in transgenic rapeseed plants may induce several oxidative-stress responsive genes to protect from cold stress.
Whitening Effect of Salvia miltorrhiza Bunge Water Extract in Human Epidermal Melanocyte
Park, Tae-Soon ; Kim, Dong-Hee ; Son, Jun-Ho ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 333~338
DOI : 10.3839/jabc.2015.052
The objective of this study was to investigate the whitening effect of Salvia miltorrhiza Bunge water extract (SM-W) in human epidermal melanocyte (HEM). Mushroom tyrosinase inhibitory effect of SM-W was approximately 42% at
. The HEM cellular tyrosinase and melanin synthesis inhibition activity were 26 and 25% at
, respectively. Whitening related proteins and mRNAs including tyrosinase, tyrosinase related protein 1 (TRP-1) and TRP-2, and microphthalmia associated transcription factor were reduced by SM-W treatment. In addition, the cAMP expression inhibitory effect of SM-W was decreased by 41% at
concentration. These results indicated that Salvia miltorrhiza Bunge could be used to the possible utilization of functional cosmetic ingredients by confirming whitening activity related with melanin content.
Effects of Extraction Time on the Quality Characteristics of Purple Corn Cob Extract
Jeong, Ji-yun ; Park, Hee-Jin ; Kim, Byung-Hee ; Kim, Sung-Soo ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 339~344
DOI : 10.3839/jabc.2015.053
In this study, the quality characteristics of purple corn cob extract (PCCE) by extraction time were examined. The cob of purple corn, which is mainly cultivated in the Andes region of South America, contains more anthocyanin than the corresponding purple corn seeds. The aim of this study was to determine the optimal conditions for the preparation of water extracts from purple corn cob. PCCE had a soluble solid content of
. The Hunter lightness (
), redness (
), yellowness (
) values of the PCCE were 31.0, 38.4, and 9.0, respectively. The PCCE contained 24.64 mg/g of cyanidin, 0.35 mg/g of pelargonidin, and 17.42 mg/g peonidin, and showed greater antioxidant activity than the other extracts. Therefore, the optimal extracting conditions for preparing PCCE were a temperature of
and an extraction time of 24 h.
Discrimination of Geographic Origin by Trace Elements Contents in Rehmannia Radix Preparat using X-ray Fluorescence Analysis
Bae, Hey-Ree ; Lee, Si-Kyung ; Whang, In-Jae ; Kang, Jeong-Mi ; Lee, Jin-Ho ; Kim, Jeong-Han ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 345~348
DOI : 10.3839/jabc.2015.054
Dried Rehmannia Radix Preparat, produced in different geographic origins of Korea and China, were investigated for the trace element contents using X-ray fluorescence spectrometry. When the estimated data for 35 kind of elements including K, P, S, Cl, Si, Al, Fe, Sn, etc on 143 samples were analyzed by canonical discrimination technique, the average classification correct rate was 92.3% and the significance was less than 0.0001. In case of reducing the number of elements for statistic analysis from 35 to 8 and to 3, the correct rates were also reduced to 88.1% and to 84.6%. The correct rate for Chinese samples ranged from 94.6-96.0%, while those for Korean ranged from 72.5-89.9%.
Various Pathogenic Pseudomonas Strains that Cause Brown Blotch Disease in Cultivated Mushrooms
Mu, Lin-Lin ; Yun, Yeong-Bae ; Park, Soo-Jin ; Cha, Jae-Soon ; Kim, Young-Kee ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 349~354
DOI : 10.3839/jabc.2015.055
Brown blotch disease in cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes a lipodepsipeptide, tolaasin. Tolaasin is a pore-forming toxin in the cell membranes, thus destroying the fruiting body structure of mushroom. In this study, we isolated pathogenic bacteria from mushrooms that had symptoms of brown blotch disease. In order to identify these bacteria, their 16S rRNA genes were sequenced and analyzed. Pathogenic bacteria identified as Pseudomonas species were thirty five and classified into five subgroups: P1 to P5. Each subgroup showed different metabolic profile measured by API 20NE kit. Fifty percent of the bacteria were identified as P. tolaasii (P1 subgroup). All five subgroups caused the formation of brown blotches on mushroom tissues and the optimum temperature was 25oC, indicating that they may be able to secrete causal factors, such as tolaasin and similar peptide toxins. These results show that there are at least five different pathogenic Pseudomonas species as blotch-causing bacteria and, therefore, strains from the P2 to P5 subgroups should be also considered and studied as pathogens in order to improve the quality and yield of mushroom production.
Development of Protein Biomarkers for the Authentication of Organic Rice
Lee, Ju-Young ; Lim, Jinkyu ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 355~361
DOI : 10.3839/jabc.2015.056
The rice protein profiles of Oryza sativa L (Koshihikari) grown under organic and conventional cultivation regimes were compared on 2-D gels to develop diagnostic marker proteins for organic rice. The selected proteins, differentially expressed between organic and conventional rice, were compared with the differentially expressed proteins of another organic and conventional rice pairing, produced at a different location. In the first comparison among conventional, no-chemical, and organic rice grown in the same region, Korea, 13 proteins exhibiting differential expression in organic and conventionally grown plants were selected. Eight of the 13 proteins were down-regulated, and the 5 remaining proteins were up-regulated from conventional to organic rice. The second comparison pairing from Kyungju, revealed 12 differentially expressed proteins, with 8 down-regulated and 4 up-regulated proteins. Ten of the differentially expressed proteins that overlapped between the two comparison sets could not be clustered into any functional group using a functional annotation clustering tool. Further comparisons using another set of conventional and organic rice, belonging to a different variety of Oryza sativa L and produced in Sanchung, revealed 8 differentially expressed proteins, 5 of which were down-regulated and 3 of which were upregulated in the organic rice. Overall, 3 differentially expressed proteins were commonly found in all three organic rice crops. These 3 proteins, along with other overlapping differentially expressed proteins, can provide a good starting point for the development of signature proteins that can be used for the authentication of organic rice with a follow-up studies with more comparison sets.
Pharmacokinetic Study of Florfenicol in Healthy and Vibriosis-infected Pseudosciaena crocea after Oral Administration
Wang, Li ; Han, Yan-nan ; Jin, Shan ; Ma, Yin ; Wang, Guo-liang ; Zhao, Qing-song ; Chen, Yin-er ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 363~368
DOI : 10.3839/jabc.2015.057
The pharmacokinetics of florfenicol were studied in healthy and vibriosis-infected large yellow croaker (Pseudosciaena crocea) following administration of a single oral dose of
. After oral administration, florfenicol levels in tissues (liver, kidney, muscle, serum, and skin) were analyzed using high-performance liquid chromatography. A two-compartment open model was used to describe the pharmacokinetics of florfenicol following oral administration. Compared to the healthy group, the absorption rate of vibriosis-infected fish significantly decreased, peak-time (
) delayed, maximum concentration (
) declined, total body clearance decreased, the elimination half-life (
) was extended, and the area under the curve increased. These results indicate that a
oral dose of florfenicol administered once daily continuously for 4 or 5 days can be used for the treatment of Vibrio alginolyticus infection in large yellow croaker (Pseudosciaena crocea).
Comparison of Fecal Microbial Communities between White and Black Pigs
Guevarra, Robin B. ; Kim, Jungman ; Nguyen, Son G. ; Unno, Tatsuya ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 369~375
DOI : 10.3839/jabc.2015.058
Meat from black pigs (BP) is in high demand compared with that from modern white pig (WP) breeds such as Landrace pigs owing to its high quality. However, the growth rate of black pigs is slower than that of white pig breeds. We investigated differences in the fecal microbial composition between white and black pigs to explore whether these breeds differed in the composition of their gut microbial communities. The swine gut microbiota was investigated using Illumina's MiSeq-based sequencing technology by targeting the V4 region of the 16S rRNA gene. Our results showed that the composition of the gut microbiota was significantly different between the two pig breeds. While the composition of the WP microbiota shifted according to the growth stage, fewer shifts in composition were observed for the BP gut microbiota. In addition, the WP gut microbiota showed a higher Firmicutes/Bacteroidetes ratio compared with that of BP. A high ratio between these phyla was previously reported as an obesity-linked microbiota composition. Moreover, the WP microbiota contained a significantly higher abundance of cellulolytic bacteria, suggesting a possibility of higher fiber digestion efficiency in WP compared to BP. These findings may be important factors affecting growth performance and energy-harvesting capacities in pigs. Our findings of differences in the gut microbiota composition between the two breeds may provide new leads to understand growth rate variation across pig breeds.
Analysis of the 6-gingerol Content in Zingiber spp. and their Commercial Foods using HPLC
Cho, Sunghun ; Lee, Dong Gu ; Lee, Sullim ; Chae, Sungwook ; Lee, Sanghyun ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 377~381
DOI : 10.3839/jabc.2015.059
The content analysis of 6-gingerol, which is an active compound, in Zingiber spp. (Z. officinale and Z. mioga) and their commercial foods (ginger teas and powders) was conducted using high-performance liquid chromatography. A reverse phase system was used, with a gradient solvent system of water and acetonitrile. The 6-gingerol content was highest in the methanol extract of Z. officinale root (17.09 mg/g extract) and ginger powder B (15.92 mg/g extract). The results demonstrated that this method was simple and reliable for the quality control of Zingiber commercial foods.
Development of Duplex PCR Method for Simultaneous Detection of Rabbit (Oryctolagus cuniculus) and Cat (Felis catus) Meats
Hong, Yeun ; Kim, Mi-Ju ; Yang, Seung-Min ; Yoo, In-Suk ; Kim, Hae-Yeong ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 383~387
DOI : 10.3839/jabc.2015.060
A duplex polymerase chain reaction (PCR) detection method was developed to authenticate the use of cat and rabbit in food and to prevent unlawful distribution of illegally butchered meat in both domestic and imported food market. Species-specific primers were designed targeting mitochondrial cytochrome b gene. The sizes of PCR products were 191 bp for cat and 101 bp for rabbit, which were relatively small for better application of the detection method on processed foods. Specificities of primers were verified using 21 animal species including cat and rabbit. Limit of detection was examined by serial dilution of the sample DNA and confirmed as 0.005 ng for rabbit and 0.0005 ng for cat using Bioanalyzer. The developed duplex PCR method showed specificity and sensitivity in the identification of two target species.
Erratum to: Anticancer Activities of the Methanolic Extract from Lemon Leaves in Human Breast Cancer Stem Cells
Moon, Jeong Yong ; Nguyen, Linh Thi Thao ; Hyun, Ho Bong ; Osman, Ahmed ; Ahn, Kwang Seok ; Kim Cho, Somi ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 389~389
DOI : 10.3839/jabc.2015.062
Erratum to: Alkyl Glycosides from the Flowers of Magnolia obovata
Oh, Eun-Ji ; Seo, Kyeong-Hwa ; Kwon, Jung-Hwa ; Lee, Dae-Young ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 58, issue 4, 2015, Pages 391~391
DOI : 10.3839/jabc.2015.063