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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 59, Issue 2 - Jun 2016
Volume 59, Issue 1 - Mar 2016
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Comparison of Allergy-Inducible Wheat Protein Contents among Imported and Domestic Wheat Flours in Korea
Kim, Ju Hee ; Pak, Pyo June ; Kim, Jung-Gon ; Cheong, Young-Keun ; Kang, Chon-Sik ; Lee, Nam Taek ; Chung, Namhyun ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 1~3
DOI : 10.3839/jabc.2016.001
Wheat is a staple food in the Korean diet, which is increasingly becoming westernized. Because most domestic wheat consumption relies on imported wheat, we aimed to evaluate the allergy-inducible protein contents of commercial flours from imported and domestic wheat. Analysis of the protein contents by densitometry suggested that domestic wheat flours contain lower levels of high molecular weight glutenin and omega-gliadin (50 and 34% lower, respectively) than imported wheat flours. Therefore, domestic wheat flours are less likely to cause allergic reactions than imported wheat flours are. Based on the findings of our study, were commend increased consumption of domestic wheat flours to those who are sensitive to allergy.
Isolation and Identification of Major Component from Roots of Potentilla chinensis
Jung, Hae Soo ; Kim, Hyoung Shik ; Lee, Jeong Hun ; Moh, Seo Jin ; Yeo, Jin Hui ; Park, Gi won ; Moh, Sang Hyun ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 5~7
DOI : 10.3839/jabc.2016.002
One of oriental medicinal plants, Potentilla chinensis, has been used for anti-inflammation, hemostatic, decryption, and antipyretic. Especially, a root of Potentilla chinensis was used as important material for oriental medication. Although several kinds of bioactive component of Potentilla chinensis extract from stems and leaves were identified, the major component of Potentilla chinensis from roots is not well established. In this study, the root of Potentilla chinensis was extracted in different solvent system and analyzed by high performance liquid chromatography (HPLC). According to HPLC analysis, a major component was isolated and its physicochemical properties were evaluated by mass spectrometry and nuclear magnetic resonance. Based on these results, isolated compound was identified as 2,3,8-Tri-O-methylellagic acid. And quantification of 2,3,8-Tri-O-methylellagic acid with different extraction solvent system was performed for industrial application.
Characterization of Biological Degradation Cypermethrin by Bacillus amyloliquefaciens AP01
Lee, Yong-Suk ; Lee, Je-Hoon ; Hwang, Eun-Jung ; Lee, Hyo-Jung ; Kim, Jae-Hoon ; Heo, Jae Bok ; Choi, Yong-Lark ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 9~12
DOI : 10.3839/jabc.2016.003
Strain AP01 was isolated for the biological cypermethrin degradation from soil and sediment in Busan. This strain was identified on the basis of phylogenetic analysis of the 16s rDNA sequence and assigned as Bacillus amyloliquefaciens AP01. AP01 could degrade about 45% of cypermethrin in the mineral medium at
and 180 rpm for 5 days. Furthermore when 2% glucose was added in the medium, the degradation rate of cypermethrin by strain AP01 was increased upto about 60%. Therefore, AP01 may serve as a promising strain in the bioremediation of soil polluted with cypermethrin.
The Effect of Roots Extract from Potentilla chinensis as Cosmeceutical Material
You, Jae Chon ; Jung, Hae Soo ; Kim, Hyoung Shik ; Lee, Jeong Hun ; Moh, Sang Hyun ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 13~17
DOI : 10.3839/jabc.2016.004
As natural plant-based industry has been expanded, the use of oriental medicinal plants as cosmeceutical material received a lot of attentions in the cosmetic industry. Among various medicinal plants, Potentilla chinensis have drawn interests for its biological effects. Although several attempts were tried to study its biological effect as medicinal plant, only limited results were reported to evaluate its biological effect as cosmeceutical material. In this study, we examined the possibility of root extract from Potentilla chinensis as a cosmeceutical material because the root part has been reported to have several kinds of health promoting effects. After extraction of roots, biological evaluation including anti-inflammation, anti-wrinkle, whitening effect and moisturizing effect was performed. As a result, the root extract showed remarkable biological activities through stimulating synthesis of elastin and aquaporin-3, and inhibiting melanin synthesis, cyclooxygenase-2 expression and expression of metallopeptidase-1.
Inhibitory Activity against Helicobacter pylori of Isolated Compounds from Pinus koraiensis Siebold et Zucc Leaves
Jo, Bun-Sung ; Cho, Young-Je ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 19~23
DOI : 10.3839/jabc.2016.005
A phenol substance was extracted from Pinus koraiensis Siebold et Zucc leaf extracts and its biological efficacy was measured. The highest content of the phenol substance contained in Pinus koraiensis Siebold et Zucc leaves was 13.5 mg/g, which was obtained when it was extracted with 80% ethanol. At a concentration of 200 mg/mL, the phenolic substances extracted with 80% ethanol and water showed antimicrobial activities against Helicobacter pylori, producing clear zones of 10 and 12 mm diameter, respectively. Pinus koraiensis Siebold et Zucc. leaf extracts were separated using a Sephadex LH-20 column and 4 fractions were obtained (fractions A-D). Fractions C and D showed the greatest inhibitory activity against Helicobacter pylori producing 10.1 and 12.3 mm clear zones, respectively. These two fractions were purified using a Sephadex LH-20 and MCI-gel column (
ethanol). Purified compounds A and B were identified as syringic acid and compound C was identified as p-coumaric acid based on
-nuclear magnetic resonance (NMR),
-NMR, and fast atom bombardment mass spectrometry spectra. When two or more purified compounds were mixed, a synergistic effect of anti-Helicobacter pylori activity was evident. This result indicates that extracts of Pinus koraiensis Siebold et Zucc leaves could be considered a functional food because of their high antimicrobial properties.
The hepatitis B virus X protein induced fibrosis in Huh7 cells
Son, Moa ; Park, Sanggyu ; Cho, Moonjae ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 25~29
DOI : 10.3839/jabc.2016.006
Hepatitis B virus infection can cause hepatic fibrosis leading to cirrhosis and hepatocellular carcinoma. However the mechanism remains poorly understood. In this study, we found that Hepatitis B virus X-protein (HBx) increases vimentin, fibronectin, slug, snail and NOX4 expression. Because NOX4-mediated reactive oxygen species can increase slug and snail, which can induce fibrosis, HBx may be a key regulator of hepatic fibrosis development via NOX4 induction.
Anticancer and Antiviral Activity of Chlorine Dioxide by Its Induction of the Reactive Oxygen Species
Kim, Yonggyun ; Kumar, Sunil ; Cheon, Wonsu ; Eo, Hyunji ; Kwon, Hyeok ; Jeon, Yongho ; Jung, Jinboo ; Kim, Wook ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 31~36
DOI : 10.3839/jabc.2016.007
Chlorine dioxide has been used for a disinfectant by exhibiting antimicrobial activity and is also potent to kill insect pests infesting stored grains. This study aimed to extend the usefulness of chlorine dioxide with respect to anticancer and antiviral activities. Cytotoxicity of chlorine dioxide was assessed against five different human cancer cell lines. Chlorine dioxide exhibited significant cytotoxicity against two breast cancer cell lines (MCF-7, MDA-MB-231) and three colorectal cancer cell lines (LoVo, HCT-116, SW-480). This cytotoxicity appeared to be associated with the capacity of chlorine dioxide to induce the production of reactive oxygen species (ROS). Compared to control insect cell lines, the cancer cell lines possessed much higher levels of ROS. On the other hand, a treatment of an antioxidant, vitamin E, significantly reduced the cytotoxicity, suggesting that the cytotoxicity was induced by high levels of ROS production. Chlorine dioxide exhibited antiviral activity against different viruses. A baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), is a dsDNA insect virus and lost its viral activity to form polyhedral viral particles in response to chlorine dioxide. The antiviral activity against AcNPV was dependent on the incubation time with chlorine dioxide. Tobacco mosaic virus is a ssRNA plant virus and was reduced in its population after exposure to chlorine dioxide along with significant decrease of viral symptoms. These results indicate that chlorine dioxide possesses anticancer and antiviral activities probably due to its inducing activity of ROS production.
Anti-inflammatory Activity of Extracts from Ultra-Fine Ground Saururus chinensis Leaves in Lipopolysaccharide-Stimulated Raw 264.7 Cells
Kim, Dong-Hee ; Cho, Jun-Hyo ; Cho, Young-Je ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 37~43
DOI : 10.3839/jabc.2016.008
Bioactive components of ultra-fine ground Saururus, the extraction yield increases when the leaves are ultra-fine ground. Comparison of normal-ground and ultra-fine ground Saururus chinensis leaves showed that the solid content and antiinflammatory activity of ultra-fine ground extracts was higher than that of normal-ground extracts. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations of Saururus chinensis extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 2 times more NO than cells that were not treated with LPS. Moreover, the NO production in cells treated with Saururus chinensis extract was inhibited in a concentration-dependent manner. Because the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we measured the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. We found that the amount of iNOS decreased dose-dependently. It was reduced by 53% at a Saururus chinensis extract concentration of
. The protein expression of cyclooxygenase-2 (COX-2) in LPS-treated Raw 264.7 cells was inhibited by 31% at
of Saururus chinensis extract. Gel shift of the nuclear factor kappa B-DNA complex occurred in LPS-treated cells and the intensity of the band decreased gradually in a concentration-dependent manner. Ultra-fine ground Saururus chinensis extract had a concentration-dependent inhibitory effect on the production of prostaglandin
, tumor necrosis factor
), IL-6, and IL-8 in LPS-treated Raw 264.7 cells, i.e., at
of Saururus chinensis extract, their levels were decreased by 53, 67, 52, 37, and 21% respectively.
Some Monascus purpureus Genomes Lack the Monacolin K Biosynthesis Locus
Kwon, Hyung-Jin ; Balakrishnan, Bijinu ; Kim, Yeon-Ki ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 45~47
DOI : 10.3839/jabc.2016.009
Two Monascus purpureus genomes lack the monacolin K biosynthesis locus (mok), while Monascus species are generally assumed to be monacolin K producers. These M. purpureus harbor a fusion of mokA and mokB orthologues. This finding suggests that an ancestral mok locus underwent a deletion event in the M. purpureus genome.
Cissus quadrangularis Extracts Decreases Body Fat Through Regulation of Fatty acid Synthesis in High-fat Diet-induced Obese Mice
Lee, Hae Jin ; Lee, Dong-Ryung ; Choi, Bong-Keun ; Park, Sung-Bum ; Jin, Ying-Yu ; Yang, Seung Hwan ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 49~56
DOI : 10.3839/jabc.2016.010
The current study investigated the anti-obesity effect of Cissus quadrangularsis extracts (CQR-300) and its molecular action mechanism on obese mice induced high-fat diet (HFD). To induce the obesity, mice were fed a HFD for 6 weeks and then fed HFD only or HFD with CQR-300 at 50 and 200 mg/kg. Then, body weight gain and white adipose tissue weights were measured. We investigated the reduction in body fat and the regulation of fatty acid synthesis was measured by dual energy X-ray absorptiometry and real-time PCR with Western blot, respectively. In vitro study, CQR-300 inhibited pancreatic lipase activity. The CQR-300 treatment was significantly decreased the body weight gain and adipocytes size as well as white adipose tissues weights in HFD-induced obese mice. Furthermore, CQR-300 reduced the body fat and fat mass with regulating of adipose tissue hormones as leptin. Treatment with 50 mg/kg CQR-300 showed effectively lower expression levels of adipogenesis/lipogenesis related genes and proteins such as CCAAT/enhancer binding protein
), peroxisome proliferator-activated receptor
), Sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) in white adipose tissue (WAT) as compared with the HFD fed only mice. These results suggest that the CQR-300 has an anti-obesity effect via inhibition of lipase activity, decrease the body fat mass by regulating the adipogenesis and lipogenesis related genes and proteins in epididymal adipose tissue with evaluate body fat reduce in the HFD-induced obese mice.
Effect of Fermentation on the Antioxidant Activity of Rice Bran by Monascus pilosus KCCM60084
Cheng, Jinhua ; Choi, Bong-Keun ; Yang, Seung Hwan ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 57~62
DOI : 10.3839/jabc.2016.011
In this study, we optimized fermentation conditions for the solid state fermentation of rice bran with Monascus pilosus KCCM60084, and the antioxidant activities were investigated. Optimal fermentation conditions were determined by the production of Monacolin K, a functional secondary metabolites with cholesterol lowering activity. The highest Monacolin K production were 2.88 mg/g observed on day 10 with 45% moisture content in the substrate when inoculated with 5% inoculum (w/w). Reducing power, iron chelating activity and
radical scavenging activity were significantly enhanced after fermentation by 60, 80, and 38% respectively. Furthermore, the content of total flavonoid were found to be increased by 4.58 fold. Based on these results, Monascus-fermented rice bran showed strong possibility to be used as a natural antioxidant agent due to its enhanced antioxidant activity.
Analysis of Foodborne Pathogens in Brassica campestris var. narinosa microgreen from Harvesting and Processing Steps
Oh, Tae Young ; Baek, Seung-Youb ; Choi, Jeong Hee ; Jeong, Moon Cheol ; Koo, Ok Kyung ; Kim, Seung Min ; Kim, Hyun Jung ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 63~68
DOI : 10.3839/jabc.2016.012
This study was performed to assess the microbiological quality of Brassica campestris var. narinosa microgreen from harvesting and processing steps. The samples were analyzed for total viable cell counts (TVC), coliforms, Enterobacteriaceae, Escherichia coli, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus, and Staphylococcus aureus. The total viable counts of microgreen (whole leaves) and environment samples from harvesting steps were higher than 6.8 log CFU/g and the contamination level of coliforms in the samples were 3.2 log CFU/g and 3.5 log CFU/g of microgreen and soil, respectively. In case of microgreen samples collected from processing steps, the contamination level of TVC and coliforms were higher in raw materials than samples obtained from later stages of processing, i.e. washing, drain, and final products. The contamination levels of B. cereus in raw materials and environments decreased approximately 1.4 log CFU/g in final products. S. aureus was detected in soil samples but Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus and pathogenic E. coli was not detected. In order to identify the sources of contamination for microgreen, the genetic similarity of B. cereus isolates obtained from harvesting and processing steps were compared using the repetitive-sequence-based polymerase chain reaction method. B. cereus isolates obtained from harvesting environments and microgreen were clustered with a similarity greater than 95%. In case of B. cereus isolates obtained from microgreen and environmental samples at processing steps showed low genetic similarity.
Combined Treatment of Fumaric Acid with Mild Heat to Inactivate Microorganisms on Fresh Spinach during Storage
Son, Hyeon-Jeong ; Kang, Ji-Hoon ; Oh, Deog-Hwan ; Min, Sea Cheol ; Song, Kyung Bin ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 69~74
DOI : 10.3839/jabc.2016.013
The objective of this study was to examine the combined effect of fumaric acid with mild heat on the inactivation of microorganisms on spinach. Spinach leaves were inoculated with Escherichia coli O157:H7 and Listeria monocytogenes. Based on the results of single treatment of fumaric acid (0.1, 0.3, and 0.5%) or mild heat (40, 50, and
) regarding the inactivation of the inoculated bacteria, the optimal condition for the combined treatment was suggested to be 0.5% fumaric acid and mild heat treatment at
for 5 min. The combined treatment of fumaric acid with mild heat caused 2.53 and 2.62 log reductions of the populations of L. monocytogenes and E. coli O157:H7, respectively. In addition, during storage of fresh spinach at
for 12 d, the combined treatment reduced initially the populations of total aerobic bacteria by 2.77 log CFU/g compared with the control. In particular, after 12 d of storage, the population of total aerobic bacteria for the combined treatment sample was 4.84 log CFU/g, whereas the control sample had 6.66 log CFU/g. Color and vitamin C content of spinach samples were not altered significantly by the combined treatment during storage. These results indicate that the combined treatment of fumaric acid with mild heat is an effective method to control microorganisms on spinach during storage.
Immobilization of α-amylase from Exiguobacterium sp. DAU5 on Chitosan and Chitosan-carbon Bead: Its Properties
Fang, Shujun ; Chang, Jie ; Lee, Yong-Suk ; Hwang, Eun-Jung ; Heo, Jae Bok ; Choi, Yong-Lark ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 75~81
DOI : 10.3839/jabc.2016.014
Glutaraldehyde was used as a cross-linking agent for immobilization of purified
-amylase from Exiguobacterium sp. DAU5. Befitting concentration of glutaradehyde and cross-linking time is the key to preparation of cross-linking chitosan beads. Based on optimized immobilization condition for
-amylase, an overall yield of 56% with specific activity of 2,240 U/g on chitosan beads and 58% with specific activity of 2,320 U/g on chitosan-carbon beads was obtained. The optimal temperature and pH of each immobilized enzyme activity were
and 50 mM glycine-NaOH buffer pH 8.5, respectively. Those retained more than 75 and 90% of its maximal enzyme activity at pH 7.0-9.5 and after incubation at
for 1 h, respectively. In addition, the immobilization product showed higher organic-solvent tolerance than free enzymes. The mode of hydrolyzing soluble starch revealed that the
-amylase possessed high hydrolyzing activity. These results indicate that chitosan is good support and has broad application prospects of enzyme immobilization.
Heavy Metal Contamination in Surface Water Used for Irrigation: Functional Assessment of the Turag River in Bangladesh
Arefin, M. Taufique ; Rahman, M. Mokhlesur ; Wahid-U-Zzaman, M. ; Kim, Jang-Eok ;
Journal of Applied Biological Chemistry, volume 59, issue 1, 2016, Pages 83~90
DOI : 10.3839/jabc.2016.015
The aim of the present study was to evaluate the degree of metal contamination of the Turag River water and its suitability for irrigation. Twenty water samples were analyzed for physicochemical parameters and metals viz., calcium, magnesium, potassium (K), sodium, copper (Cu), zinc (Zn), iron (Fe), manganese (Mn), lead (Pb), cadmium (Cd), chromium (Cr), and nickel (Ni). All water samples were slightly alkaline to alkaline. Regarding electrical conductivity (EC), all samples were suitable for crop in soils with moderate permeability and leaching. Water samples were medium salinity and low alkalinity hazard classes. In terms of total dissolved solids (TDS), all samples were classified as freshwater. As per sodium adsorption ratio (SAR) and soluble sodium percentage (SSP), all samples were classified as excellent. No residual sodium carbonate (RSC) was detected in any of the samples, indicating suitability for irrigation; and all samples were considered very hard. Cr and Mn contents in all samples were above FAO guideline values and, therefore, these metals were considered toxic. Zn, Cu, Pb, Cd, and Ni concentrations were below acceptable limit for irrigation and do not pose a threat to soil environment. Significant relationships were found between EC and TDS, SAR and SSP, SAR and RSC, and SSP and RSC. The combinations of ions such as K-Zn, K-Fe, K-Cu, K-Mn, K-Pb, Zn-Fe, Zn-Cu, Zn-Mn, Fe-Mn, Cu-Mn, Cu-Pb and Mn-Pb exhibited significant correlation. This study revealed that Turag River water samples are contaminated with Cr and Mn. This fact should not be ignored because water contamination by metals may pose a threat to human health through food chain.