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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Applied Biological Chemistry
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Journal DOI :
The Korean Society for Applied Biological Chemisty
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Volume & Issues
Volume 59, Issue 2 - Jun 2016
Volume 59, Issue 1 - Mar 2016
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Diels-Alder type adducts from the fruits of Morus alba L.
Lee, Yeong-Geun ; Seo, Kyeong-Hwa ; Hong, Eock-Kee ; Kim, Dong-Man ; Kim, Young-Eon ; Baek, Nam-In ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 91~94
DOI : 10.3839/jabc.2016.016
The fruits of Morus alba L. were extracted with 80 % aqueous MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated silica gel (
) and octadecyl silica gel column chromatographies for the EtOAc and n-butyl alcohol fractions led to isolation of two phenolic compounds. The chemical structures of the compounds were determined as Diels-Alder type adducts, mulberrofuran E (1) and chalcomoracin (2) based on spectroscopic data analyses including nuclear magnetic resonance, mass spectrometry, and infrared spectrometry. Compounds 1 and 2 were isolated for the first time from the fruits of M. alba L. in this study.
Phototactic behavior 10: phototactic behavioral effects of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) adults to different light-emitting diodes of seven wavelengths
Park, Jun-Hwan ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 95~98
DOI : 10.3839/jabc.2016.017
Phototactic behavioral responses of the Indian meal moth, Plodia interpunctella (
), adults were determined to different light-emitting diodes (LEDs) of seven wavelengths, and their behavioral responses were compared to that using a commercial luring lamp (BLB) under laboratory conditions. Based on the attractive responses under optimal light conditions (60 lx luminance intensity and 30 min light exposure time), the green LED (
) showed the highest attractive rate (
, 52.2 %), followed by the blue LED (
, 33.9 %), the yellow LED (
, 32.2 %), BLB (28.9 %), UV LED (365 nm, 22.8 %), the red LED (
, 14.5 %), the white LED (450-620 nm, 10.6 %), and IR LED (730 nm, 9.5 %). In addition, the green LED to P. interpunctella adults was approximately 1.81 times more attractive than BLB. These results indicate that the green LED could be most useful for monitoring of P. interpunctella adults.
Phototactic behavior 9: phototactic behavioral response of Tribolium castaneum (Herbst) to light-emitting diodes of seven different wavelengths
Song, Jaeun ; Jeong, Eun-Young ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 99~102
DOI : 10.3839/jabc.2016.018
The phototactic behavioral responses of Tribolium castaneum adults to light-emitting diodes (LEDs) of seven different wavelengths were determined under various conditions (light exposure times, light sources, and luminance intensities) and compared with those of a black light bulb (BLB) under laboratory conditions. Based on the attractive rate (%) of T. castaneum adults under optimal conditions (50 lx and an 48 h exposure time) in the dark, red LED (
) exhibited the highest potential attractive rate (97.8 %), followed by yellow (
, 68.9 %), green (
, 55.6 %), infrared (IR) (730 nm, 54.4 %), white (450-620 nm, 41.1 %), blue (
, 34.4 %), and ultraviolet (UV) (365 nm, 0.06 %) LEDs. In comparison, red LED (97.8 %) was approximately 3.4 times more attractive to T. castaneum adults than the BLB (28.9 %). These results indicate that a red LED trap could be useful to control T. castaneum adults.
Phenolic compounds from the leaves of eggplant (Solanum melongena L.)
Baek, Dong-Ryeol ; Lee, Min-Jee ; Baek, Nam-In ; Seo, Kyeong-Hwa ; Lee, Youn-Hyung ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 103~106
DOI : 10.3839/jabc.2016.019
The leaves of eggplant (Solanum melongena L.) were extracted with 80 % aqueous MeOH, and the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH, and water fractions. From the n-BuOH fraction, five compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies. On the basis of physic-chemical and spectroscopic data including mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance, they were identified to be caffeic acid (1), chlorogenic acid (2), cryptochlorogenic acid (3), panasenoside (4), and (6R,7E,9R)-4,7-megastigmadien-3-one-9-
-glucopyranoside (5). Compounds 3 and 4 were isolated for the first time from the leaves of S. melongena L. in this study.
Determination of pectolinarin in Cirsium spp. using HPLC/UV analysis
Cho, Sunghun ; Lee, Jaemin ; Lee, Yoon Kyoung ; Chung, Mi Ja ; Kwon, Ki Han ; Lee, Sanghyun ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 107~112
DOI : 10.3839/jabc.2016.020
Pectolinarin was isolated from the ethyl acetate fraction of Cirsium setidens using open column chromatography and was analyzed using spectrometry. Pectolinarin content in Cirsium spp. was determined using HPLC/UV. Pectolinarin content in the aerial part of Cirsium spp. was higher than that in the root and pappus. Pectolinarin content was highest in the aerial part of C. chlorolepis (110.65mg/g extract). Consequently, the aerial part of C. chlorolepis has potential for use in new natural medicinal products, health supplements, and beverages.
Preventive effect of fermented red ginseng on cisplatin-induced nephrotoxicity mouse
Hyun, Ja-Kyoung ; Kwon, O Jun ; Lee, Joo Young ; Roh, Seong-Soo ; Seo, Young-Bae ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 113~124
DOI : 10.3839/jabc.2016.021
Red ginseng is known to have many beneficial effects. Cisplatin, an effective antineoplastic drug, can cause many side effects like irreversible sensorineural hearing loss and serious tinnitus in humans. This study is aimed to reduce a cisplatin's side effect, nephrotoxicity by fermentated korean red ginseng. Korea ginseng was produced by steaming and dring and fermentation. And mice were divided into 4 groups- (A) normal mice, (B) Vehicle treated cisplatin mice, (C) RG0F0-treated cisplatin mice, (D) RG8F3-treated cisplatin mice. C and D groups were feed each material 200 mg/kg/day during 4 days. And cisplatin 20 mg/kg injected to B, C, and D groups as abdominal injection. After 24 h, blood sample was collected. The kidneys were harvested for histological, immuno histochemical and western blot analysis. 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity was depended on steaming hours. RG0F0 and RG8F3 (ginseng-8 h steamed and fermented by Saccharomyces cerevisiae) were showed antioxidants effect in DPPH and ABTS radical scavenging activity. Component amounts according to steaming hours. 8 h steamed red ginseng had the most ingredients of ginsenoside. Treatments with RG8F3 reduced cisplatin-induced nephrotoxicity in the mice resulting in increase of GSH and decrease of ROS, BUN, creatinine, and inflammatory mediators. This result seems to be involved with the restriction of the inflammation in the kidney. Therefore, fermented red ginseng might have therapeutic efficacy in reduce kidney injury induced by cisplatin treatment.
Acaricidal activity and chemical composition of essential oil derived from the Albizziae julibrissin barks
Park, Jun-Hwan ; Lee, Sang-Guei ; Kim, Jeong-Moon ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 125~128
DOI : 10.3839/jabc.2016.022
The chemical compositions of the essential oil extracted from Albizziae julibrissin barks were analyzed by Gas chromatography-Mass spectrometry spectrometry. Fourteen components were identified, representing 89.23 % of the total oil composition. The analysis of the essential oil revealed that the essential oil contains 14 compounds, accounting for 89.23 % of the total oil. Hexanoic acid was the principal component (41.43 %) of the essential oil, followed by 4,4,6-trimethyl-cyclohex-2-en-1-ol (11.16 %), palmitic acid (9.00 %), 2-pentylfuran (5.66 %), 2-butyl-2-octenal (4.12 %), linoleic acid (3.10%), amyl hexanoate (3.01%), (E,E)-2,4-decadienal (2.49 %), 2-hexylthiophene (2.47 %), caprylic acid (2.13 %),
(1.52 %), heptanoic acid (1.27 %), 3,5-octadien-2-ol (0.99 %), and 2-octenal (0.88 %). The acaricidal activity of the A. julibrissin oil was tested against Dermatophagoides farina, D. pteronyssinus and Tyrophagus putrescentiae by the fumigant bioassay. Based on the
values, the essential oil exhibited strong acaricidal activities against D. farinae (
), D. pteronyssinus (
), and T. putrescentiae (
). These results indicate that A. julibrissin oil could be a source of acaricidal agents for mite control.
Effect of LED trap on controlling Sitophilus zeamais and Tribolium castaneum in granary
Song, Ja-Eun ; Lee, Sang-Guei ; Lee, Hoi-Seon ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 129~132
DOI : 10.3839/jabc.2016.023
This study was conducted to evaluate the attraction effects of Sitophilus zeamais and Tribolium castaneum to light emitting diode (LED) trap in granary and compared with the black light bulb (BLB) trap, which is typical used in commercial trap. The red LED trap showed more attractive to S. zeamais and T. castaneum than that of the BLB. Moreover, the external condition of granary was about 1.5 times more attractive to S. zeamais and T. castaneum than the internal condition of granary. These results suggested that red LED trap could be useful to control S. zeamais and T. castaneum in granary.
Anti-oxidative and anti-proliferative activities of acetone extract of the cortex of Ulmus pumila L.
In, Man-Jin ; Kim, Dong Chung ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 133~136
DOI : 10.3839/jabc.2016.024
An acetone extract in the cortex of Ulmus pumila L. was prepared to evaluate its anti-oxidative and anti-proliferative activities. The free radical scavenging activity (
) and reducing power (
) proportionally increased according to the extract concentration. The acetone extract possessed a potent anti-proliferative activity against human non-small cell lung cancer (A549,
) and human colon cancer (SNU-C4,
) cells in a dose-dependent manner, but was less effective with human normal cells (L132, human embryonic lung epithelial cell).
In vivo and In vitro hair growth promotion effects of extract from Glycine soja Siebold et Zucc
Yang, Jae Chan ; Kim, Bo Ae ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 137~143
DOI : 10.3839/jabc.2016.025
Hair is a dermal adjunctive organ that protects the body from external physical and chemical stimuli; hair undergoes anagen, catagen, and telogen phases, with hair-loss occurring during the telogen phase. Alopecia is a condition wherein a person undergoes hair-loss far exceeding the normal amount, owing to diverse external factors. Wild beans are rich in isoflavone and amino acids known to prevent hair-loss; compared to cultivated beans, many wild bean species have higher protein content. This study aimed to develop a hair growth promoting solution, with superior hair growth promoting effects and fewer side effects, using naturally obtained Glycine soja Siebold et Zucc (GSSZ) extracts. Seven-week-old C57BL/6N male mice were classified into different experimental groups. Hair growth was observed in GSSZ-treated mice, and compared against that seen in 3 % minoxidil (MXD, positive control)-treated mice. Visual observations revealed a greater reduction in hair-loss in MXD and GSSZ application groups, compared to that in TXN group (hair loss induction using 1 % testosterone). Evaluation using an image analysis software revealed that compared to the positive control, TXN + GSSZ group showed the highest hair growth. TXN + MXD and control groups exhibited similar follicular cell growth, while the hair growth promotion patterns were similar in the negative control (normal), TXN + GSSZ, and TXN groups, as observed via histological analysis. GSSZ did not induce cytotoxicity (even at 2 mg/mL) in keratinocytes and dermal papilla cells; alternately, dermal papilla cell proliferation was activated in a (GSSZ) concentration-dependent manner. Therefore, the GSSZ extract promoted hair growth and increased hair growth-related cell activity, and could therefore be utilized in alopecia treatment.
Evaluation of the cytotoxicity of gold nanoparticle-quercetin complex and its potential as a drug delivery vesicle
Pak, Pyo June ; Go, Eun Byeol ; Hwang, Min Hee ; Lee, Dong Gun ; Cho, Mi Ju ; Joo, Yong Hoon ; Chung, Namhyun ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 145~147
DOI : 10.3839/jabc.2016.026
Recently, conjugates of medicinal herb-derived bioflavonoids, such as quercetin, and gold nanoparticles (GNPs) have gained attention as targeted drug delivery systems. In the present study, because quercetin is an important flavonoid with anti-cancer, anti-inflammatory, and anti-oxidant properties, GNP-quercetin complexes (GNPQs) were synthesized to investigate possible adverse effects such as cytotoxicity. We found that while quercetin was cytotoxic, GNPQs were not cytotoxic towards the RAW 264.7 and THP-1 cell lines. Therefore, GNPQs may serve as a potential drug delivery system for cancer treatment.
Endophytic Bacillus subtilis MJMP2 from Kimchi inhibits Xanthomonas oryzae pv. oryzae, the pathogen of Rice bacterial blight disease
Cheng, Jinhua ; Jaiswal, Kumar Sagar ; Yang, Seung Hwan ; Suh, Joo-Won ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 149~154
DOI : 10.3839/jabc.2016.027
An endophytic bacterial strain was isolated from kimchi, a Korean traditional fermented Brassica campestris and identified as Bacillus subtilis MJMP2 based on the 16S rRNA sequence. This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease, as well as activity against some other rice phytopathogenic fungi. The active compound was purified through size-exclusion chromatography and preparative High-performance liquid chromatography. The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A. Furthermore, a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay. The crude extract also enhanced the length of roots of Arabidopsis thaliana. These results suggest that the strain Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease.
Triterpenoids from the fruits of Prunus davidiana
Lee, Min-Jee ; Kim, Ji-Hye ; Cha, Byeong-Ju ; Seo, Kyeong-Hwa ; Baek, Nam-In ; Lee, Youn-Hyung ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 155~158
DOI : 10.3839/jabc.2016.028
The fruits of Prunus davidiana were extracted with 80 % aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-butyl alcohol, and water fractions. From the EtOAc fraction, three triterpenoids were isolated through the repeated silica gel (
) and octadecyl
column chromatographies. Based on physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the compounds were identified to be ursolic acid (1), corosolic acid (2), and
Benzaldehyde as a new class plant growth regulator on Brassica campestris
Choi, Geun-Hyoung ; Ro, Jin-Ho ; Park, Byoung-Jun ; Lee, Deuk-Yeong ; Cheong, Mi-Sun ; Lee, Dong-Yeol ; Seo, Woo-Duck ; Kim, Jin Hyo ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 159~164
DOI : 10.3839/jabc.2016.029
Plant growth regulator is an essential pesticide to date while the available active ingredient is not well understood unlike fungicide, insecticide and herbicide. This study was aimed to evaluate a new chemical class of plant growth regulator, and the total of 92 benzene derivatives were screened for their germination and early stage of the root growth regulation on Brassica campestris. Thirty benzaldehydes, nine acids, one amide, and one ester showed potent root growth inhibitory activity (>70 % inhibition) while only salicylaldehyde showed potent germination inhibition (
) suggesting that benzaldehyde was a key module candidate for the growth inhibition. Benzaldehydes were further evaluated for root growth inhibition. 2,3-Dihydroxybenzaldehyde and salicylaldehyde showed
values of 8.0 and 83.9 mg/L, respectively. On the other hand, salicylaldehyde, and 2,4,5-trihydroxybenzaldehyde were found to have root growth promotion effects less than 10 mg/L. This result suggests that the benzaldehyde is a new class candidate for plant growth regulator.
Quantitative analyses of ricinoleic acid and ricinine in Ricinus communis extracts and its biopesticides
Choi, Geun Hyoung ; Kim, Leesun ; Lee, Deuk Yeong ; Jin, Cho long ; Lim, Sung-Jin ; Park, Byung Jun ; Cho, Nam-Jun ; Kim, Jin-Hyo ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 165~169
DOI : 10.3839/jabc.2016.030
The quantitative analytical method for the bioactive substance, 3-cyano-4-methoxy-N-methyl-2-pyridone (ricinine) and an index compound, ricinoleic acid in castor plant (Ricinus communis) extract or oil was developed. For the determination of a pyridone alkaloid compound, ricinine, successive cartridge cleanup method combined with ultra-performance liquid chromatography was set up with
(0.5 g) and
SPE cartridges. Accuracy and precision were evaluated through fortification studies of one biopesticide (PE) at 10 and
. Mean recoveries of ricinine were 98.7 and 96.0 % associated with less than 10 % RSD, respectively. For the determination of ricinoleic acid in castor extract and oil, saponification and methylation were optimized using gas chromatography-time of flight mass spectrometry. Recovery was more than 84.8 % associated with 6.2 % RSD after derivatization procedure. Both methodologies developed were applied to analyze real samples including three castor oil products and six commercially available biopesticides containing R. communis, collected at Korean market. The contents of ricinine and ricinoleic acid in most commercial biopesticides were less than the oil or extract contents indicated by label.
Erratum to: Anticancer Activities of the Methanolic Extract from Lemon Leaves inHuman Breast Cancer Stem Cells
Moon, Jeong Yong ; Nguyen, Linh Thi Thao ; Hyun, Ho Bong ; Osman, Ahmed ; Ahn, Kwang Seok ; Kim Cho, Somi ;
Journal of Applied Biological Chemistry, volume 59, issue 2, 2016, Pages 171~171
DOI : 10.3839/jabc.2016.031