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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Plant Pathology Journal
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 15, Issue 6 - Dec 1999
Volume 15, Issue 5 - Oct 1999
Volume 15, Issue 4 - Aug 1999
Volume 15, Issue 3 - Jun 1999
Volume 15, Issue 2 - Apr 1999
Volume 15, Issue 1 - Feb 1999
Selecting the target year
Toxicity of Tomato Spotted Wilt Virus Glycoprotein Signal Peptide and Promoter Activity of th 5' UTR
Park, Tae-Jin ; Kim, Sun-Chang ; Thomas L. German ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 313~318
Cloning of the 5'untranslated region (5' UTR) and Nterminus of the glycoprotein precursor (G2G1) open reading frame of tomato spotted wilt virus has been problematic, possibly because of the toxicity of a signal peptide at the beginning of th G2G1 protein precursor. The toxicity of the signal peptide to bacterial growth and the reason for the expression of the peptide gene in Escherichia coli were investigated by cloning the 5' UTR and the signal peptide sequence separately. Cells transformed with the plasmid containing both the first 30 amino acids of the glycoprotein and the 5' UTR showed a severe growth inhibition whereas transformants harboring either the plasmid with the signal sequence or the 5'UTR alone did not show any ingibition. An E. coli promoter-like sequence was found in the 5'UTR and tis promoter acivity was confirmed with a promoter-less GUS gene cloned downstream of the 5'UTR. In the cloning of the Tomato spotted wilt virus (TSWV) glycoprotein G2G1 open reading frame all the recovered plasmids contained stop codons in the signal sequence region. However, clones containing no stop codon were recovered when the signal sequence and the 5'UTR were cloned separately.
Ethyl Acetate Extract of Bacillus pumilus SH122 Induces Resistance Against Phytophthora Blight in Pepper Plant
Lee, Seoung-Hee ; Cha, Jae-Soon ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 319~322
In order to obtain bacterial metabolites inducing disease resistance in pepper plant, two hundred bacterial isolates were isolated from the rhizosphere soil of tobacco, cucumber, and pepper plant. Ethyl acetate extract of each bacterial culture was used to screening for induction of resistance against phytophthora blight of pepper plant. Application of ethyl acetate extract of an isolate SH122 culture to pepper plant conferred resistance against phytophthora blight consistently and significantly. According to cellular fatty acid analysis and other characteristics, the SH122 culture were significantly lower than those on control plants treated with ethyl acetate extract of nutrient broth. The B. pumilus SH122 itself of ethyl acetate extract of its culture did not show antifungal activity against phytophthora blight in pepper plants.
Comparison of Pepper Anthracnose Pathogens from Korea and China
Shin, Hyun-Joo ; Chen, Zhu-Jun ; Hwang, Jae-Moon ; Lee, Soon-Gu ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 323~329
Anthracnose of pepper casued by Colletotrichum spphas been a great problems for pepper production in Korea and China. Especially Colletotrichum gloeosporioides was found predominantly over cultivation areas during infection periods and caused severe rots on bath unripe and ripe fruits that resulted in major yield losses. In this study, comparison of Colletrichum spp.isolated from Korea and China in morphology and pathgenicity, and RAPD-PCR analysis were conducted. Based on morphological characteristics, the pathogen isolates, K1 and C1, K2 and C2, and K3 and C3 were identified as Colletotrichum gloeosporioides (G) type, C. gloeosporioides (R) type and C. coccodes, respectively. in pathogenicity test, K1 and C1, and K2 and C2 were found to attack mainly fruits and to be the most virulent among isolates. K3 and C3 were strongly virulent to leaves and seedling. Pathogenicity between Korean and Chinese isolates. K3 and C3 were strongly virulent to leaves and seedling. Pathogenicity between Korean and Chinese isolates did not show any difference. Results of the RAPD-PCR analyses indicate the varying levels of molecular diversity within and between Colletotrichum spp.of Korea and China. The similarities between K1 and C1, K2 and C2, and K3 and C3 were 85.71%, 71.43% and 50.0% respectively.
Pycnidiospore Production and Dispersal from the Warts Produced by Infection of Botryosphaeria dothidea on Apple Stems
Park, Chang-Hee ; Yang, Hee-Jung ; Hyun Woo ; Kim, Dai-Gee ; Uhm, Jae-Youl ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 330~334
Applying the method of quantitative analysis of pycnidiospore from the detached warts produced by the infection of Botryosphaeria dothidea on apple stems, repeated productivity of spores within the detached warts, variations in the amount of spores within the detached warts, variations in the amount of spores by the length of induction time for sporulation, and the effects of temperature and moisture on the sporulation were investigated. In addition to these experiment, the changes in the state of spores within the pycnidia contained in the warts accompanied by the induction of sporulation and dispersal of spores were also investigated. When detached warts were kept in moist conditions, the sporulation and discharge of spores were also investigated. When detached warts were kept in moist conditions, the sporulation and discharge of spores could be repeated several times, and the amount of spores were almost constant after each repeat of sporulation induction and dispersal of spores in a given period. The fact that the pycnidia filled with spores were observed at considerable rates within the warts which were subjected to the shaking in the water to release spores indicated that the spores might never be released until the pycnidia were fully matured. From the high rate of empty pycnidia even in the warts which were kept in moist conditions for induction of sporulation, the pycnidiospores might be produced through the development of new pycnidia. A considerable amount of pycnidiospores were produced at
, and the sporulation was accelerated with the rise of temperature until
. When the warts were supplied with sufficient moisture, sporulation was further accelerated. The results obtained in these experiment will be applied in developing the method for assessing the inhibitory efficacies of fungicides on the sporulation of this fungus, with which a new control measure would be developed.
Detection of Citrus Tristeza Virus by RT-PCR and Status of CTV Infection among Citrus Trees in Cheju Island
Oh, Hyun-Jeong ; Park, Sung-Hugh ; Lee, Se-Yong ; Jeon, Gyeong-Lyong ; Riu, Key-Zung ; U, Zanh-Kual ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 335~339
Citrus tristeza virus(CTV), an aphid-borne closterovirus, is one of the most destructive pathogens of citrus. It has caused rapid decline in growth, stem pitting and death in citrus trees. A reverse transcriptase polymerase chain reaction (RT-PCR) assay was developed for detection of CTV and investigation of the CTV infection status of citrus and its related cultivars in Cheju island. For RT-PCR based CTV detection, primers were designed to amplify 670bp of coat protein gene. A screening test for CTV in citrus cultivars was conducted from March to July in 1999. Seventy individual citrus trees representing 9 species of 3 genera were tested. The infection rates of CTV for leaves from the years or older trees of late maturing citrus varieties such as Yuzu (C. junos Sieb. ex Tanaka), Navel orange (C.sinensis Osbeck), Kiyomitanger (C. unshiu x C. sinensis), and Shiranuhi ((C. unshiu x C. sinensis) x C. reticulata) were 100%, 80%, 60%, and 60% respectively. The CTV infection rates in Early satsuma mandarins such as 'Miyagawa Early' Satsuma mandarins (C. unshiu Marc. var. Miyagawa) and 'Okitsu Early' Satsuma mandarins (C. unshiu Marc. var. Okitsu) were 100%, and 60%, respectively. CTV was not detected in Cheju native Dangyooja (C. unshiu Marc. var. Osbeck), Trifoliate orange (Poncirus trifoliata) and Kumquat (Fortunella margarita Swingle). In conclusion, RT-PCR assay can be successfully applied to the detection of CTV in citrus trees.
Biological Characteristics of Benzimidazole-Resistant and-Senstive Isolates of Monilinia fructicola from Peach Fruits in Korea
Lim, Tae-Heon ; Chang, Tae-Hyun ; Byeongjin Cha ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 340~344
Fungicide-resistant isolates of Monilinia fructicola grew readily on media amended with 0.1, 1.0, 10, 100 and
a.i./ml of carendazim, benomyl, or thiophanate-methyl. However, sensitive isolates did not grow on media amended even with
a.i./ml of carbendazim,
a.i./ml of benomyl or thiophanate-methyl. The fitness compositions including mycelial growth on fungicide-free medium, sporulation on fungicide-free medium and pear, and virulence on pear were not different between resistant and sensitive isolates. The resistant isolates persisted carbendazim resistance during multiple subdulturing and long term storage. The competitive ability of resistant isolates obtained from peach orchards in Korea was similar to those of sensitive isolates.
Occurrence of Eggplant Scab Caused by Cladosporium cucumerinum in Korea
Kwon, Jin-Hyeuk ; Kang, Soo-Woong ; Park, Chang-Seuk ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 345~347
A scab disease on eggplant (Solanum melongena L. cv. Chukyang) in plastic film houses around Kimhae area in Korea during the winter season of 0998-1999. The disease started on leaves with small dark brown spots which were gradually expanded to 1 to 3 mm diameter lesions. Later, the central parts of the lesions became collapsed and detached to make holes. Dark brown mold was grown out of the lesions on the lower side of leaf. Numerous conidia were produced on the lower side of leaf. Numerous conidia were produced on the diseased leaves and appeared to be readily dispersed in the air. A fungus was isolated from the diseased leaves, and tested for Koch's postulates to prove the causal agent of the desease. The isolated fungus grew on potato dextrose agar, forming greenish black to pale brown colonies. Conidia were ellipsoidal, fusiform or subspherical, mostly one-celled but occasionally septated, and formed in long branched chains on the erected conidiophores which were pale olevaceous brown and variable in length between 12.4 and
. The fungus was identified as Cladosporium cucumerinum Ellis Arthur based on the above morphological characteristics examined. The optimum temperature for mycelial growth and conidial formation was about 20 to
. In addition to cucumber, the fungus was also pathogenic to watermelon, pumpkin and oriental melon. This is the first report on the scab disease of eggplant in Korea.
Gray Leaf Spot of Tomato Caused by Stephylium solani
Kim, Byung-Soo ; Cho, Hyun-Jung ; Hwang, Hee-Suk ; Cha, Yu-Sun ;
The Plant Pathology Journal, volume 15, issue 6, 1999, Pages 348~350
Gray leaf spot of tomato was observed on tomato plants growing in a middle of a pepper field where pepper plants were badly infected with gray leaf spot. Both Stemphylium solani and S. lycopersici were isolated from the lesion. Both species were highly pathogenic on tomato and pepper. This is the first report in Korea of occurrence of gray leaf spot of tomato caused by Stemphylium solani Weber.