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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Plant Pathology Journal
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
Editor in Chief :
Volume & Issues
Volume 16, Issue 4 - Aug 2000
Volume 16, Issue 3 - Jun 2000
Volume 16, Issue 2 - Apr 2000
Volume 16, Issue 1 - Feb 2000
Volume 16, Issue 6 - Jan 2000
Volume 16, Issue 5 - Jan 2000
Selecting the target year
Three Different Viruses Isolated from Typical Weed Plants that Grown Adjacent to Common Crop Fields
Kwon, Sun-Jung ; Choi, Hong-Soo ; Han, Jung-Heon ; La, Yong-Joon ; Kim, Kook-Hyung ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 297~305
Weeds are widely grown in the field and are infected by many viruses. A survey was conducted to identify viruses infecting weeds in Korea. Virus-infected weed samples including Rorippa indica (L.) Hiern, R. islandica (Oed.) Bord, Crepidiastrum denticulatum (Houtt.) Pak & Kawanno, Achyranthes japonica (Miq.) Nakai, and Chrysanthemum boreale (Makino) Makino were collected in Kyonggi Province. These weeds were grown in the greenhouse and were isolated on 10 test plants. Several virus isolates were isolated fron infected tissues and were further studied by host range assay, serological test, electron microscopy (EM), reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Each isolated virus strain was mechanically transmitted to weeds and various hosts including Nicotiana spp., Brassica spp., Vigna unguiculata, Capsicum annuum, and Cucumis sativus and showed systemic mosaic, vein clearing, necrosis, mottle, malformation, chlorosis, and/or death of host plants in some cases. Each virus was then purified using infected leaves and observed by EM. From these results three viruses were isolated and identified as Turnip mosaic virus (TuMV), Broad bean wilt virus (BBWV), and Cucumber mosaic virus (CMV). RT-PCR using virus-specific oligonucleotide primers and the cloning were conducted to determine the nucleotide sequences of coat proteins of the three viruses their amino acid sequence were deduced. The amino acid sequence homologies were about 92.7 to 99.7%, 96.2 to 97.7%, and 93.9 to 98.6% to other reported TuMV, BBWV, and CMV strains, respectively. These results suggest that many weeds may serve as primary inoculum source of diseases caused by TuMV, BBWV, CMV and that the management of these viral diseases can be achieved through weed control.
A Novel Strain of Cucumber mosaic virus Isolated from Lilium longiflorum
Jung, Hye-Jin ; Ueda, Shigenori ; Ryu, Ki-Hyun ; Lee, Sang-Yong ; Choi, Jang-Kyung ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 306~311
A new strain of Cucumber mosaic virus (CMV) from easter lily (Lilium longiflorum), Ly2-CMV, was identified and compared to the well-characterized Mf-CMV (subgroupⅠ) and LS-CMV (subgroupⅡ) by host reaction in several indicator plants, dsRNA analysis, serological property, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein (CP) gene. Remarkable differences in symptoms of Ly2-CMV were found between Mf-CMV or LS-CMV in tobacco plants and Datura stramoinium. Ly2-CMV induced small necrotic ringspots on the inoculated leaves of Nicotiana tabacum cvs. Xanthi nc and Burley 21 and D. stramonium, and failed to infect these species systemically. Of the indicator plants tested, N. benthamiana only reacted with systemic infection by inoculation of Lr2-CMV. In experiments of dsRNA analysis, serology and RT-PCR of CP gene, Ly2-CMV was come within subgroupⅠ CMV. However, restriction enzyme analysis of the PCR products using MspⅠ showed that Ly2-CMV was distinct to Mf-CMV. The CP gene of Ly2-CMV contains 657 nucleotides, and the nucleotide sequence is similar to that of Mf-CMV. There is also a high degree of conservation between their putative gene products in Ly2-CMV and Mf-CMV, with five amino acid changes in the 218 amino acids of the CPs.
Induction of Systemic Resistance in Watermelon to Gummy Stem Rot by Plant Growth-Promoting Rhizobacteria
Lee, Yong-Hoon ; Lee, Wang-Hyu ; Shim, Hyeong-Kwon ; Lee, Du-Ku ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 312~317
The selected five plant growth-promoting rhizobacteria (PGPR) strains, WR8-3 (Pseudomonas fluorescens), WR8-6 (P. putida), WR9-9 (P. fluorescens), WR9-11 (Pseudomonas sp.), and WR9-16 (P. putida) isolated in the rhizosphere of watermelon plants were tested on their growth promotion and control effect against gummy stem rot of watermelon. Strains, WR8-3 and WR9-16 significantly increased stem length of watermelon, and there was a little increase in leaf area, fresh weight and root length when strains, WR8-3, WR9-9 and WR9-16 were treated. Generally, seed treatment was better for plant growth promotion than the soil drench, but there was no significant difference. Seed treatment and soil drench of each bacterial strain also significantly reduced the mean lesion area (MLA) by gummy stem rot, but there was no significant difference between the two treatments. At initial inoculum densities of each strain ranging from 10
cfu/g seed, approximately the same level of disease resistance was induced. But resistance induction was not induced at the initial inoculum density of 10
cfu/g seed. Resistance was induced by treating the strains, WR9-9, WR9-11 and WR9-16, on all of four watermelon varieties tested, and there was no significant difference in the decrease of gummy stem rot among varieties. Populations of the strains treated initially at log 9-10 cfu/g seed, followed with a rapid decrease from planting day to 1 week after planting, but the population density was maintained above log 5.0 cfu/g soil until 4 weeks after planting. Generally no or very weak in vitro antagonism was observed at the strains treated excepting WR9-11. Rifampicin-resistant bacteria which had been inoculated were not detected in the stems or leaves, which suggesting that the bacterium and the pathogens remained spatially separated during the experiment. This is the first report of rsistance induction in watermelon to gummy stem rot by PGPR strains.
Silver Scurf of Potato Caused by Helminthosporium solani
Ryu, Kyoung-Yul ; Hahm, Young-Il ; Kim, Jeom-Soon ; Park, Chun-Soo ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 318~320
Potate tubers with slver scurf lesions were collected from the cold storage at Pyungchang, Kangwon province in Korea. The causal agent of the silver scurf was identified as Helminthosporium solani by mycological characteristics of conidia and conidiophores. Pathogenicity of the fungus was confirmed by artificial inoculation on the potato tuber. This is the first report of potato silver scarf by Helminthosporium solani in Korea.
Occurrence of Rhizopus Soft Rot on Squash (Cucurbita moschata) Caused by Rhizopus stolonifer in Korea
Kwon, Jin-Hyeuk ; Kang, Soo-Woong ; Park, Chang-Seuk ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 321~324
season of 2000. The disease infection usually started from flower, peduncle and young fruits, then moved to flower stalk, stem and leaves. At first, the lesions started with water-soaked, rapidly softened, and then the area gradually expanded. In severely affected film house, the rate of infected fruits reached to 28.6%. Numerous sporangiospores were formed on the diseased fruits, flower stalk, stem and leaves. Most of the sporangiospores were appeare to be rapidly dispersed in the air. The mycelia grew on the surface of host and formed stolons. Colonies on potato dextrose agar were cottony at first brownish black at maturity. Sporangia were 125.3
m. globose or sub-globose with somewhat flattened base. White at first the black, many spored, and are never overhanging. Sporangiophores were 2.7-6.8
m, smooth-walled, non-septate, light brown, simple, long, arising in groups of 3-5 from stolons opposite rhizoids. Sporangiophores were 8.6-21.1
m, irregular, round, oval, elongate, angular and brownish-black streaked. Columella were 63.8
m. brownish gray, umberella-shaped when dehisced. The causal organism was identified as Rhizopus stolonifer Lind on the basis of the morphological characteristics of the fungus. Rhizopus soft rot on squash (Cucurbita moschata) caused by the fungi has not been previously reported in Korea.
Leaf Spot of Rye Caused by Bipolaris sorokiniana in Korea
Chang, Seog-Won ; Hwang, Byung-Kook ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 325~327
A leaf spot of rye (Secale cereale L.) was observed during the summer 1999 in Korea. A fungus associated with the disease was identified as Bipolaris sorokiniana (Sacc.) Shoem., based on the morphological characteristics of conidia and conidiophores. Pathogenicity of the fungus was proven by artificial inoculation on rye plants. This is the first record of leaf spot on rye caused by B. sorokiniana in Korea.
Occurrence of Target Leaf Spot of Red and White Clovers Caused by Stemphylium sarciniforme in Korea
Cho, Hye-Sun ; Yu, Seung-Hun ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 328~330
A terget leaf spot of red and white clovers was observed during 1998-2000 growing seasons in several fields of Chungnam and Chungbuk provinces in Korea. Lesions were circular to oval in outline, brown to dark brown, ranging from a pinpoint to 3-4 mm in diameter, often concentrically zonate and presenting a target effect. A fungus associated with the disease was identified as Stemphylium sarciniforme based on the morphological characteristics of the conidiophores and conidia. The fungus was pathogenic on red and white clovers in the inoculation test. This is the first record of a terget leaf spot of red and white clovers in Korea.
Three Alternaria Species Pathogenic to Sunflower
Cho, Hye-Sun ; Yu, Seung-Hun ;
The Plant Pathology Journal, volume 16, issue 6, 2000, Pages 331~334
Alternaria helianthi and two unreported species of Alternaria in Korea were isolated from lesions of Alternaria leaf spot disease of sunflower. The unrecorded species of Alternaria were identified as A. helianthinficients and A. protenta based on the morphological characteristics of conidiophores and conidia. A. helianthi was the dominant species, although all the three species were associated with the disease. A. helianthi, A. helianthinficiens and A. protenta produced similar symptoms on detached sunflower leaves. This is the first report of A. helianthinficiens and A. protenta pathogenic on sunflower in Korea.