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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Plant Pathology Journal
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 17, Issue 6 - Dec 2001
Volume 17, Issue 5 - Oct 2001
Volume 17, Issue 4 - Aug 2001
Volume 17, Issue 3 - Jun 2001
Volume 17, Issue 2 - Apr 2001
Volume 17, Issue 1 - Feb 2001
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Differentiation of Phytoplasmas Infecting Zizyphus jujuba and Paulownia coreana Using PCR-RELP
Han, Mu-Seok ; Noh, Eun-Woon ; Yun, Jeong-Koo ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 189~193
The relationships between the phytoplasmas infecting Zizyphus jujuba and Paulownia coreana were investigated by PCR-RELP. The 16S rRNA genes of the phytoplasmas were analyzed and compared with each other after PCR amplification. The amplified bands 1.4 kb in size were analyzed by both restriction digestion and sequencing after cloning into a plasmid vector. In some cases, two different kinds of inserts were observed in the isolates that originated from a single plant. However, many of them appeared to be the amplification products of chloroplastic 16S rRNA gene of host plants. The phytoplasma gene could be differentiated from the chloroplastic gene by restriction digestion of the plasmids carrying the amplification products. Only the recombinant plasmids carrying phytoplasma 16S rRNA gene produced a 1.4 kb band when digested with the enzyme BanII. Of the 52 recombinant plasmids analyzed, 42 appeared to contain inserts that originated from the chloroplastic 16S rRNA gene of the host plants. No variation was detected among 16S rRNA gene of nine phytoplasma isolates infecting Z. jujuba. However, the phytoplasmas infecting Z. jujuba were different from that infecting P. coreana.
Chrysanthemum stunt viroid in Dendranthema grandiflorum
Chung, Bong-Nam ; Park, Gug-Seoun ; Kim, Hyun-Ran ; Kim, Jeong-Soo ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 194~200
Chrysanthemum stunt viroid (CSVd) ws identified in chrysanthemum cv. Chunkwang showing symptoms of stunt with leaf distortion (K1) and stunt with chlorosis of leaves (K2) collected from the main cultivation area of Masan, Kyongnam province in Korea. The specific RNAs related with the diseased chrysanthemums were detected. Full-length 354 bp CSVd cDNAs were amplified from infected tissue by reverse transcription and polymerase chain reaction using a pair of primers specific for CSVd sequence. The amplified cDNA products were analyzed by agarose gel electrophoresis and the specific cDNAs were cloned. Nucleotide sequences of the two CSVd isolates K1 and K2 varied. Phylogenetic analysis of the nucleotide sequences of CSVd isolates indicated that K1 was closely related with J2 and Am 2 isolates. K1 and K2 were transmitted by grafting to Dendranthema grandiflorum cv. Mistletoe, Gynura aurantiaca, and Lycopersicon esculentum cv. Rutgers. This is the first report of CSVd in D. grandiflorum in Korea.
Ultrastructural Aspects of the Mixed Infections with Turnip mosaic virus and Ribgrass mosaic virus in Oriental Cabbage
Kim, Jeong-Soo ; Cho, Jeom-Deog ; Park, Hong-Soo ; Kim, Kyung-Soo ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 201~204
Ultrastructural observation was conducted for the cells of oriental cabbage, Brassica campestris ssp. pekinensis 'Chungawang', inoculated simultaneously with Turnip mosaic virus (TuMV-ACT2-4vq) and Ribgrass mosaic virus (RMV-Ca1dn2) which were known as major destructive viruses of oriental cabbage in Korea. In cells infected with RMV alone, the virus particles were located as bundle or scattering in cytosols and vacuoles, which were typical ultrastructures of tobamovirus. Vessels of xylem were compacted with RMV particles. The cells infected only with TuMV had the cluster of virus particles scarcely and the typical potyvirus inclusions of scrolls, pinwheels, tubes and laminated aggregates in cytosols. The TuMV particles were jammed lineally between tonoplasts. In double infection, the two unrelated viruses of TuMV-ACT2-4vq and RMV-CA1dn2 were located together in a cell, and typical properties of each virus were also observed. The potyvirus inclusions and the tobamovirus particles were mixed entirely in cytoplasm. The virus particles of RMV wre presented strikingly near and in the center of potyvirus inclusions. In vascular cells, the tobamovirus particles were located abundantly than those in single infection. The potyvirus inclusions were embedded in the cluster of RMV particles in phloem parenchyma cells and the vascular elements were degenerated severely.
Fitness of Dicarboximide-Resistant and Sensitive Monilinia fructicola Isolated from Peach in Korea
Lim, Tae-Heon ; Yi, Jae-Choon ; Chang, Tae-Hyun ; Byeongjin Cha ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 205~209
Dicarboximide-resistant isolates of Monilinia fructicola grew readily on media amended with dicarbosimide fungicides, and showed cross-resistance to pentachlo-ronitrobenzene (PCNB). The fitness of resistant isolates was inferior to that of sensitive isolates. Mycelial growth on fungicide-free medium was not significantly different between the dicarboximide-resistant and sensitive isolates. The originally high
values of the resistant isolate decreased after storage for 16 weeks at
. After inoculation with the mixture of spore suspensions of resistant and sensitive isolates, the re-isolation rate of the resistant spores was significantly reduced regardless of the mixing ratio. From the results, it could be concluded that the competitive ability of the resistant isolates is inferior to the sensitive ones.
Mycelial Melanization of Rhizoctonia solani AG1 Affecting Pathogenicity in Rice
Kim, Heung-Tae ; Chung, Young-Ryun ; Cho, Kwang-Yun ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 210~215
The phenotype of Rhizoctonia solani KR-13 was randomly segregated to both melanin-producing (M+) and non-producing (M-) types through successive cultures on PDA. M+type with dark melanin showed strong pathogenicity to rice and self-anastomosis. Meanwhile, M- type with white or less-melanized mycelia showed very weak pathogenicity and non-self-anastomosis. Melanin production of R. solani was affected by incubation temperature in both M+ and M- types, but not by light treatment. The application of tricyclazole, an inhibitor of fungal melanin biosynthesis, showed no controlling effect on R. solani causing rice sheath blight. Results of this study showed that melanization of mycelia of R. solani is an important pathogenicity factor in rice.
Comparison of Cenangium Dieback Fungus Isolated from Three Different Species of Pine
Jung, Joo-Hae ; Lee, Sang-Yong ; Lee, Jong-Kyu ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 216~221
Dieback of pine branches or twigs with brown needles occurs most commonly on Pinus species after severe winter in Korea. In this study, Cenangium ferruginosum was isolated from infected stems, branches, and twigs of Pinus koraiensis (C1), P. densiflora (C2), and P. thunbergii (C3). Morphological and cultural characteristics of the isolates were than compared. There were no significant differences in the morphological characteristics of conidia and ascospores produced by the three isolates. However, cultural differences were observed among the isolates. Optimum temperatures for mycelial growth of C1, C2, and C3 were 15, 20, and
, respectively. C1 produced a few conidia and no ascospores, while C2 and C3 produced abundant ascospores and conidia. While optimum temperatures for mycelial growth ranged from 15 to
, mycelial growth was also relatively good at lower temperatures of 5-
. Conidiomata and conidia were produced on MSA (malt extract soya peptone agar) after 25-30 days of incubation in the dark at
. Apothecia were produced by altering culture condition from 15 to
, and incubating for 35-60 more days. Optimum temperature for ascospore and conidium germination was
. RAPD analysis revealed that there was high similarity of 0.78 between C2 and C3, and low similarity of 0.31 between C2 or C3 and C1.
Identification of Verticillium dahliae and V. albo-atrum Causing Wilt of Tomato in Korea
Kim, Jong-Tae ; Park, In-Hee ; Lee, Hyang-Burm ; Hahm, Young-Il ; Yu, Seung-Hum ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 222~226
In 1997, 103 isolates of Verticillium were obtained from roots and stems of tomato plants showing wilt symptoms in greenhouses in eight areas of Korea. Out of these isolates, 75 were edintified as V. dahliae while 28 were identified as V. albo-atrum based on their morpho-logical and cultural characteristics. Both Verticillium species produced colonies with conidiophores, which were more or less erect, hyaline, with verticillate branches, and with 3-4 phialides at each node. V. dahliae produced microsclerotia, while V. albo-atrum produced resting dark mycelium. Optimum temperatures for mycelial growth of V. dahliae and V. albo-atrum on PDA were 22 and
, respectively. Mycelial growth of V. albo-atrum was slower than that of V. dahliae. Pathoge-nicity tests revealed that tomato cvs. Zuikoh No. 102, Kyoryokubeiju No. 2, Zuiken, Kagimuza, and Momotaro were susceptible to V. albo-atrum, while cvs. Zuikoh No. 102 and Kyoryokubeiju No.2 were susceptible to V. dahliae.
Selection of Resistant Hybrids of Atractylis Against Phytophthora drechsleri
Kim, Dong-Kil ; Shim, Chang-Ki ; Kim, Hee-Kyu ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 227~230
Bioassay techniques using young leaves and roots were developed to screen resistance of Atractylis spp. against Phytophthora drechsleri. Among 638 plants collected from various regions of Korea from 1994 to 1996, 67 were pre-screened in fields naturally infested with P. drechsleri, which is the causal pathogen of rhizome rot of Atractylis. Among the pre-screened sources, 18 (ca. 26.8%) were highly resistant to the pathogen in leaf inoculation. In the root inoculation test, abundant sporangia were formed in susceptible plant roots, while only a few or no sporangia were produced on the roots which were found resistant in the leaf inoculation test. Among the selected resistant plants, A. japonica 96066 and 96104 were used to cross with another species, A. macrocephala 96362 that showed high yield with good quality of rhizome but susceptible to the pathogen. The F
hybrids designated as HA03 turned out to be resistant to the pathogen, indicating that resistant gene(s) was inherited. Among intra-species hybrids of A. japonica, HA07 and HA09 were resistant to the pathogen in leaf inoculation and moderate in root inoculation. However, HA08 was susceptible in both inoculation tests. This result suggests that the parent material might be genetically heterogeneous. Further genetic study should be carried out to verify this phenomenon.
Outbreak of Phytophthora Rot on Pear Under Environmental Conditions Favorable to the Disease
Jee, Hyeong-Jin ; Cho, Weon-Dae ; Nam, Ki-Woong ; Park, Young-Seob ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 231~235
From April to May 1998, Phytophthora rot on pear, which has not been reported in Korea before, became an epidemic in the southeast part of the country under abnormally higher temperature and prolonged rainy days. Average temperature was about
higher than in normal years, and 29 days were rainy during the 2 months in the areas surveyed. Over 1,000 orchards estimated at about 270 ha in 19 cultivation areas were infected by the disease, which occurred on all parts of the tree such as leaves, shoots, branches, stems, and flower clusters. Among 43 isolates collected from various locations and plant parts, 41 were identified as Phytophthora cactorum while 2 were identified as P. cambivora based on their mycological characteristics. The representative isolates revealed strong pathogenicity not only to pear but also to apple and peach. Among 23 pear cultivars tested, 7 were estimated as susceptible, 4 were moderate, and 11 were resistant to the pathogen. Results suggest that Phytophthora disease on pear is a potential threat to pear cultivation when environmental factors are favorable to disease development.
Anthracnose of Perilla Caused by Colletotrichum spp. and Glomerella cingulata
Kim, Wan-Gyu ; Lee, Byung-Dae ; Cho, Weon-Dae ; Shin, Dong-Bum ;
The Plant Pathology Journal, volume 17, issue 4, 2001, Pages 236~241
Serve outbreaks of anthracnose were observed on perilla plants grown in greenhouses and open fields in several locations in Korea during the disease survey from 1997 to 2000. A total of 53 isolates of Colletotrichum spp. and Glomerella sp. was obtained from diseased perilla plants and identified based on their morphological and cultural characteristics. Forty isolates were identified as Colletotrichum gloeosporioides, three isolates as C. coccodes, five isolates as C. dematium, and the other five isolates as Glomerella cingulata, the teleomorph of C. gloeosporioides. All isolates of C. gloeosporioides tested by artificial inoculation were strongly virulent on perilla plants, but isolates of the other species were weakly or not virulent. Anthracnose symptoms induced on the perilla plants by artificial inoculation with the isolates of C. gloeosporioides were similar to those observed in the fields. This study revealed that C. gloeosporioides is the main causal fungus of perilla anthracnose.