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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Plant Pathology Journal
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Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 18, Issue 6 - Dec 2002
Volume 18, Issue 4 - Aug 2002
Volume 18, Issue 3 - Jun 2002
Volume 18, Issue 2 - Apr 2002
Volume 18, Issue 1 - Feb 2002
Volume 18, Issue 5 - Jan 2002
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Phylogenetic rind Taxonomic Status of the Phytoplasmas Associated with Water Dropwort (Oenanthe javanica DC) Disease in Korea and Japan
Jung, Hee-Young ; Woo, Tae-Ha ; Hibi, Tadaaki ; Namba, Shigetou ; Lee, Joon-Tak ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 109~114
DOI : 10.5423/PPJ.2002.18.3.109
To evaluate the phylogenetic and taxonomic status of the phytoplasmas associated with water dropwort (Oenanthe javanica DC) disease in Korea and Japan, their 16S rDNA was analyzed. DNAs extracted from water dropworts collected in Korea (Kyongnam province) and Japan (Chiba prefecture) affected by witches' broom and yellows were subjected to PCR using phytoplasma-specific primers, which amplified a 1.4-kbp fragment that included the 16S rDNA. Phytoplasmas were characterized by RFLP analysis using AluI, HaeIII, HhaI, KpnI, MseI, and RsaI restriction enzymes and by sequence analysis of the PCR products. The mater dropwort witches'broom (WDWB) and water dropwort yellows (WDY) 16S rDNA sequences were identical and closely related to onion yellows (OY, 99.9% identity), which belong to the aster yellows (AY) 16S-subgroup. However, the KpnI RFLP analyses clearly distinguished the WDY and WDWB phytoplasmas from the OY phytoplasma. The phylogenetic analysis based on 16S rDNA showed that WDWE and WDY phytoplasmas are members of a relatively homogeneous group that evolved from a common ancestor.
Subcellular Responses in Nonhost Plant Infected with Pathogenic and Non-pathogenic Strains of Xanthomonas axonopodis pv. glycines
Jeong, Yong-Ho ; Kim, Jung-Gun ; Chang, Sung-Pae ; Hwang, In-Gyu ; Kim, Young-Ho ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 115~120
DOI : 10.5423/PPJ.2002.18.3.115
Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule of soybean, induces hypersensitive response (HR) in a non-host plant, hot pepper (Capsicum annuum). A wild-type strain (8ra) and its non-patho-genic mutant (8-13) of X. axonopodis pv. glycines were inoculated into the pepper leaf tissues and their subcellular responses to the bacterial infections were examined by electron microscopy. Intrastructural changes related to HR were found in the leaf tissues infected with 8ra from 8 h after inoculation, characterized by separation of plasmalemma from the cell wall, formation of small vacuoles and vesicles, formation of cell wall apposition, and cellular necrosis. No such responses were observed in the tissues infected with the mutant. In 8ra, the bacterial cells were attached to the cell walls, with the cell wall material dissolved into and appearing to encapsulate the bacterial cells. The bacterial cells later became entirely embedded in the cell wall material. On the other hand, in 8-13, the bacterial cells were usually not attached tightly to the plant cell wall, and no or poor encapsulation of the bacteria by the wall material occurred, although these were encircled by rather loose wall materials at the later stages.
First Report of Zucchini yellow mosaic virus on Hollyhock (Althaea rosea)
Park, Won-Mok ; Park, Seung-Kook ; Yoon, Ju-Yeon ; Ryu, Ki-Hyun ; Park, Jang-Kyung ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 121~125
DOI : 10.5423/PPJ.2002.18.3.121
This study was conducted to determine the causal virus that naturally infected hollyhock (Althaea rosea) plant showing mild mosaic symptom in 1999. Flexuous virus particles were found in the cytoplasm of plant tissue from infected hollyhock under transmissible electron microscopy. A virus from the genus Potyvirus under the family Potyviridae was isolated and was maintained on Chenopodium quinoa for three passages. Chlorotic local legions were used to inoculate 20 species of indicator plants. The virus infected all the tested cucurbit plants, but failed to infect Nicotiana benthamiana. Based on the host range test and RT-PCR analysis, the potyvirus was identified as a strain of Zucchini yellow mosaic virus-A (ZYMV-A), one of the major pathogens of cucurbits. Infectivity analysis showed that ZYMV-A induced faster systemic symptom than ZYMV-Cu on squash and other cucurbit plants, suggesting that ZYMV-A was a more severe strain. To better characterize ZYMV-A, Western blot assay was carried rout to the coat protein (CP) of the virus using ZYMV-specific antiserum with ZYMV-Cu and other potyviruses. The CP of the virus reacted strongly with the antiserum against ZYMV, and other tested antisera did not react with the CP of ZYMV-A. Results strongly suggest that the potyvirus infecting hollyhock was a novel strain of ZYMV. This is the first report on ZYMV as the causal virus infecting hollyhock in Korea.
First Report of Sweet potato latent virus and Sweet potato chlorotic stunt virus Isolated from Sweet Potato in Korea
Yun, W.S. ; Lee, Y.H. ; Kim, K.H. ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 126~129
DOI : 10.5423/PPJ.2002.18.3.126
Infected sweet potato (Ipomoea batatas) showing symptoms of sunken veins, stunting, mosaic, and mottling were collected from Gimje, Cochang, Iksan, and Haenam provinces in Korea. Electron microscopic (EM) observation of the infected tissue revealed rod and filamentous rod type virus particles of various lengths. Western blot analysis of the protein samples extracted from infected sweet potato and partially purified virus identified the isolates as Sweet potato feathery motile virus (SPFMV), Sweet potato latent virus (SwPLV), and Sweet potato chlorotic stint virus (SPCSV). Sweet potatoes were occasionally infected with more than one of these viruses. This is the first report of SwPLV and SPCSV in Korea.
Variation of Potato virus Y Isolated from Potato, Tobacco, Pea and Weeds in Korea on the C-terminal Region of Coat Protein Gene and 3'Non-translated Region
Yun, W.S. ; Jung, H.W. ; Oh, M.H. ; Hahm, Y.I. ; Kim, K.H. ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 130~137
DOI : 10.5423/PPJ.2002.18.3.130
Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV
, and PV
. In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.
Serratia plymuthica Strain A2l-4: A Potential Biocontrol Agent Against Phytophthora Blight of Pepper
Shen, Shun-Shan ; Kim, Jin-Woo ; Park, Chang-Seuk ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 138~141
DOI : 10.5423/PPJ.2002.18.3.138
A promising biocontrol agent, A2l-4, against Phytophthora blight of pepper was selected from 351 bacterial isolates collected from rhizosphere soils and roots of onion (Allium fistulosum L.). The isolate A21-4 was identified as Serratia plymuthica based on its 16S rRNA sequence and key characteristics as compared with that of an authentic culture of S. plymuthica (ATCC No. 6109D01). The isolate readily colonized on roots of various crops including pepper when inoculated on seed and not. Strain A2l-4 showed narrow spectrum of antibiotic activity, as revealed in its strong inhibitory activity to the genera Pythium and Phytophthora, but not to Fuasrium and Rhizoctonia. In pot experiments, none of the pepper seedlings treated with A2l-4 were infected by Phytophthora capsici, while 86% of the control plants were killed by the pathogen.
Effect of Potassium Phosphonate on the Control of Phytophthora Root Rot of Lettuce in Hydroponics
Jee, Hyeong-Jin ; Cho, Weon-Dae ; Kim, Choong-Hoe ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 142~146
DOI : 10.5423/PPJ.2002.18.3.142
The effect of potassium phosphonate (
) on the control of Phytophthora root rot of lettuce was evaluated in a liquid hydroponic culture. Phosphonate 100 ppm strongly inhibited mycelial growth of Phytophthora species in vitro but did not affect normal growth of lettuce in a greenhouse test. Application of the chemical before infection showed over 94% control value, while it was less than 35% when applied after infection. In a field trial, phosphonate 100 ppm, which was directly supplemented into the nutrient solution, satisfactorily controlled the disease as it did not develop until 28 days after transplanting and remained at less than 2% infection rate at the end of cultivation. Meanwhile, in the control plot, the disease initiated at 7 days after transplanting and developed rapidly reaching over 70% infection rate at 28 days. Population density of the causal pathogen, R drechsleri, in a heavily infested farm was 22.0-25.0 cfu/100 ml of nutrient solution. However, it decreased to 1.3-2.0 cfu/100 ml at 7 days after treatment with phosphonate 200 ppm.
Effects of Fungicide Control of Downy Mildew (Pseudoperonospora cubensis) on Yield and Disease Management of Ridge Gourd (Luffa acutangula)
Deadman, M.L. ; Kagadi, S.R. ; Pawar, D.R. ; Gadre, U.A. ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 147~151
DOI : 10.5423/PPJ.2002.18.3.147
Seven fungicides were compared for the control of downy mildew on midge gourd. All treatments had significantly lower rates of disease progress curves and disease severity levels than that of the control. The highest yields were obtained from crops treated with metalaxyl + mancozeb, fosetyl-Al, and chlorothalonil. These treatments also proved to be the most economical considering the treatment costs.
Distribution and Changes in Occurrence of Fingerprint Stem Blight of Eleocharis kuroguwai Caused by Epicoccosorus nematosporus in Korea
Hong, Yeon-Kyu ; Ryu, Kil-Lim ; Hyun, Jong-Nae ; Uhm, Jae-Youl ; Kim, Soon-Chul ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 152~155
DOI : 10.5423/PPJ.2002.18.3.152
Epicoccosorus nematosporus was detected in all 22 counties surveyed, but the frequency of occurrence of fingerprint stem blight disease (FSBD) on Eleocharis kuroguwai caused by the fungus varied with fields and regions. The disease occurred more frequently in mountain areas than in plain areas. E. nematosporus was detected in less than 20% of plain areas such as Cimjae and Milyang, whereas, it occurred in 40-60% of mountain areas such as Sangiu and Jangsoo. In Milyang, mean temperature in July to August 1993 ranged from 17 to
with 14.3 h of dew period. Meanwhile, in the mountain area such as Sangiu, Gyeongbook, temperature ranged from 17 to
with 16.1 h of dew period. Plant mortality was 61% in Milyang and 82% in Sangju. Underground tuber formation was highly suppressed at 16 and 33 tubers per plot in Sangju and Milyang, respectively. In 1992 and 1995, plants infected ranged from 40 to 78% in July to September. This sharply decreased to an average of 15% in 1999.
Occurrence of Dry Rot on Cymbidium Orchids Caused by Fusarium spry. in Korea
Kim, Wan-Gyu ; Lee, Byung-Dae ; Cho, Weong-Dae ; Sung, Jae-Mo ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 156~160
DOI : 10.5423/PPJ.2002.18.3.156
Cymbidium orchids with blight and rot symptoms were collected, and a total of 63 isolates of Fusarium app. was obtained from pseudobulbs, roots, and leaves of the diseased plants. The isolates were identified based on their morphological characteristics. Out of the 63 isolates of Fusatium sup., 51 isolates were identified as F. oxysporum, 10 isolates as F. solani, and the rest as F. proliferatum. F. oxysporum was isolated from all the Cymbidium spp., while F. solani and F. proliferatum were isolated only from Cymbidium ensifolium and C. ginatum, respectively. Isolates of the three Fusarium spp. were tested for pathogenicity to their hosts by artificial inoculation. The strongly pathogenic isolates of Fusarium spp. induced severe dry rot of pseudobulbs and roots of the host plants. The symptoms progressed up to the basal part of the leaves, which later caused blight of the entire plant. The dry root symptoms induced on the plants by artificial inoculation with the isolates of Fusarium app. were similar to those observed in the growers'greenhouses. This is the first report of dry rot of Cymbidium spp. caused by F. oxysporum, F. solani, and F. proliferatum in Korea.
Isolation and Identification of Colletorichum musae from Imported Bananas
Lim, Jin-Young ; Lim, Tae-Heon ; Cha, Byeong-Jin ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 161~164
DOI : 10.5423/PPJ.2002.18.3.161
Colletotrichum musae was isolated from dark-brown anthracnose lesions on commercial banana (Musa sapientum L.) to establish the causal agent of the symptom. The fungus grew fast and produced white aerial mycelium on PDA. Acervuli developed abundantly on culture plates after incubation for 10 days at
. Pinkish conidial masses were produced on the acervuli, which mostly coalesced together, Conidia were aseptate, hyaline, straight, ellipsoid to globose, and 14.5
in size. Black, clavate, round, or irregular-shaped appressoria measuring 8.8
were readily formed from germ tubes. Setae-like structures were not found either on the lesion or on the cultures. Sclerotia were also absent. Among the media, PDA medium was the best for mycelial growth. The optimum temperature for mycelial growth was
, while the optimum pH ranged from pH 5.5 to 6.5. The isolates of C musae caused black necrotic lesions on banana fruits by needle-wound inoculation, and orange-colored spore masses were produced on the lesions. The fungus also caused discoloration on apple fruits inoculated.
Chemical Control of Leaf Spot of Date Palm (Phoenix dactylifera) in Sultanate of Oman
Livingston, Sam ; Mufargi, Khamis-Al ; Sunkeli, Mehmood-Al ;
The Plant Pathology Journal, volume 18, issue 3, 2002, Pages 165~167
DOI : 10.5423/PPJ.2002.18.3.165
Date palm (Phoenix dactylifera) is an important fruit and cash crop in Sultanate of Oman, occupying nearly 60% of the total cultivated area. However, leaf spots caused by Mycosphaerella tassiana, Alternaria spp., and Dreshcleri sp. have become a threat to date palm's cultivation in recent years. In this study, a field experiment was conducted to find out a suitable chemical spray program to control the disease. A prophylactic spray schedule with mancozeb (Dithane M45), copper oxychloride (Champion), and mancozeb+copper (Trimiltox) effectively controlled the disease when applied at a time when the disease severity index (DSI) was low, ranging from 0 to 1.68. Meanwhile, the disease did not decrease, but instead increased gradually, when the fungicide combination was applied when DSI was high, ranging from 1.78 to 5.37. It was concluded that fungicides should be applied at the early stage or before disease initiation in order to control the disease effectively.