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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Plant Pathology Journal
Journal Basic Information
Journal DOI :
Korean Society of Plant Pathology
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Volume & Issues
Volume 20, Issue 4 - Dec 2004
Volume 20, Issue 3 - Sep 2004
Volume 20, Issue 2 - Jun 2004
Volume 20, Issue 1 - Mar 2004
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Potential Biotypes in Korean Isolates of Bipolaris cactivora Associated with Stem Rot of Cactus
Kim, Jeong-Ho ; Jeoung, Myoung-Il ; Hyun, Ick-Hwa ; Kim, Young-Ho ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 165~171
DOI : 10.5423/PPJ.2004.20.3.165
A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7％ and 95.1％, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.
Rhizobacteria-mediated Induced Systemic Resistance in Cucumber Plants against Anthracnose Disease Caused by Colletotrichum orbiculare
Jeun, Yong-Chull ; Lee, Yun-Jeong ; Bae, Yeoung-Seuk ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 172~176
DOI : 10.5423/PPJ.2004.20.3.172
Bacterial isolates TRL2-3 and TRK2-2 showing anti-fungal activity in vitro test against some plant pathogens were identified as Pseudomonas putida and Micrococcus luteus, respectively. Pre-treatment with both bacterial isolates at the concentration 1.0
cfu/ml in the rhizosphere could trigger induced systemic resistance in the aerial part of cucumber plants against anthracnose caused by Colletotrichum orbiculare. However, the pre-treatment with the higher concentration at 1.0
cfu/ml of both isolates could not induce resistance after challenge inoculation with C. orbiculare. As a positive control, the treatment with DL-3 amino butyric acid caused a remarkable reduction of disease severity whereas the lesions on the leaves of untreated plants developed apparently after the fungal inoculation. From these results, it was recomended that disease control using both bacterial isolates inducing systemic resistance in the field where chemical application is forbid.
In Vitro Anti-Oomycete Activity and In Vivo Control Efficacy of Phenylacetic Acid Against Phytophthora capsici
Lee, Jung-Yeop ; Kim, Hye-Sook ; Kim, Ki-Deok ; Hwang, Byung-Kook ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 177~183
DOI : 10.5423/PPJ.2004.20.3.177
Phenylacetic acid (PAA) was evaluated for in vitro anti-oomycete activity and in vivo control efficacy against Phytophthora capsici. Microscopic observation revealed that the high level of anti-oomycete activity of PAA (10
/ml) against P. capsici is mainly due to the lytic effect on zoospores. Zoospore lysis began in the presence of 5 u
/ml of PAA and most of the zoospores were collapsed at 10
/ml. PAA showed inhibitory activity against the zoospore germination and hyphal growth of P. capsici at the concentration of 50
/ml. In the glasshouse, the protective effect of PAA against Phytophthora blight was high on pepper plants when treated just before inoculation with P. capsici. In the artificially infested field, protection of pepper plants against the Phyto-phthora epidemic was achieved at a considerable level by PAA treatment.
In Vivo Antifungal Activities of 57 Plant Extracts Against Six Plant Pathogenic Fungi
Choi, Gyung-Ja ; Jang, Kyoung-Soo ; Kim, Jin-Seok ; Lee, Seon-Woo ; Cho, Jun-Young ; Cho, Kwang-Yun ; Kim, Jin-Cheol ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 184~191
DOI : 10.5423/PPJ.2004.20.3.184
Methanol extracts of fresh materials of 57 plants were screened for in vivo antifungal activity against Magna-porthe grisea, Corticium sasaki, Botrytis cinerea, Phyto-phthora infestans, Puccinia recondita, and Blumeria graminis f. sp. hordei. Among them, seven plant extracts showed disease-control efficacy of more than 90％ against at least one of six plant diseases. None of the plant extracts was highly active against tomato gray mold. The methanol extracts of Chloranthus japonicus (roots) (CjR) and Paulownia coreana (stems) (PcS) displayed the highest antifungal activity; the CjR extract controlled the development of rice blast, rice sheath blight, and wheat leaf rust more than 90％, and tomato gray mold and tomato late blight more than 80％. The PcS extract displayed control values of more than 90 ％ against rice blast, wheat leaf rust, and barley powdery mildew and more than 80％ against tomato gray mold. The extract of PcS also had a curative activity against rice sheath blight and that of CjR had a little curative activity against rice blast. On the other hand, the extract of Rumex acetocella roots reduced specifically the development of barley powdery mildew. Further studies on the characterization of antifungal substances in antifungal plant extracts are underway and their disease-control efficacy should be examined under greenhouse and field conditions.
Leaf and Stem Blight on Columbine and Bleeding Heart Caused by Streptobotrys caulophylli
Hong, Sung-Kee ; Kim, Wan-Gyu ; Cho, Weon-Dae ; Kim, Hong-Gi ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 192~195
DOI : 10.5423/PPJ.2004.20.3.192
A survey on ornamental diseases in 2000 and 2001 showed frequent occurrence of blight symptoms on leaves and stems of columbine and bleeding heart in botanical gardens of Gangwon and Chungnam provinces in Korea. A total of 65 isolates of Streptobotrys sp. were obtained from the infected leaves and stems of the two plants. All isolates were identified as Streptobotrys caulophylli based on their morphological and cultural characteristics. Blight symptoms were induced on leaves of the two plants by artificial inoculation with the isolates. This is the first report that S. caulophylli causes leaf and stem blight on columbine and bleeding heart in the world.
Ginseng Anthracnose Caused by Colletotrichum dematium
Han, Kee-Don ; Alam, Shahidul ; Lee, Tae-Soo ; Lee, Min-Woong ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 196~199
DOI : 10.5423/PPJ.2004.20.3.196
Ginseng anthracnose was observed in Koyang area, Korea. A fungus was repeatedly isolated from leaves and stems of the infected ginseng plants and identified as Colletotrichum dematium according to the morpho-logical and cultural characteristics. The fungus showed pathogenicity on inoculated ginseng leaves. This is the first report of ginseng anthracnose caused by Colletotri-chum dematium in Korea.
Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus
Seo, Jang-Kyun ; Lim, Won-Seok ; Jeong, Ji-Hye ; Yoo, Young-Bok ; Yie, Se-Won ; Kim, Kook-Hyung ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 200~205
DOI : 10.5423/PPJ.2004.20.3.200
The partial nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus isolates ASI2223 and Suhan were determined and compared with those of mycoviruses belonging to partitiviruses and totiviruses. Partial nucleotide sequences of the purified dsRNA from ASI2223 and Suhan showed RNA-dependent RNA polymerase sequences that are closely related to those of partitiviruses, including Fusarium poae virus 1, Fusarium solani virus, Rhizoctoniasolani virus, Discula destructiva virus 2, and Oyster mushroom isometric virus 2. Specific primers were designed for RT-PCR detection of dsRNA viruses from the P. ostreatus isolate ASI2223 and Suhan. Two virus specific primer sets were found to specifically detect each virus among six sets of designed oligonucleotide primers. Collectively, these results suggest that dsRNA mycoviruses from P. ostreatus isolates ASI2223 and Suhan belong to the family Partitiviridae, although, they are not the same virus species. Our results also suggest that these virus-specific primer sets can be employed for the specific detection of each viral sequence in infected tissues.
Virus Resistant and Susceptible Transgenic Nicotiana benthamiana Plants Expressing Coat Protein Gene of Zucchini green mottle mosaic virus for LMO Safety Assessment
Kim, Min-Jea ; Choi, Sun-Hee ; Kim, Tae-Sung ; Park, Min-Hye ; Lim, Hee-Rae ; Oh, Kyung-Hee ; Kim, Tae-San ; Lee, Min-Hyo ; Ryu, Ki-Hyun ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 206~211
DOI : 10.5423/PPJ.2004.20.3.206
Transgenic Nicotiana benthamiana plants harboring coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for virus-resistant screening and complementation analysis of related viruses for environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N.benthamiana was performed by using Agrobacterium-mediated method and the pZGC-PPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and virus-susceptible N.benthamiana lines, were obtained by screening of challenging homologous virus for Tl generations. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.
Symptom Determinant as RNA3 of Lily Isolates of Cucumber mosaic virus on Zucchini Squash
Cho, Seung-Kook ; Ahn, Hong-Il ; Kim, Min-Jea ; Choi, Jang-Kyung ; Ryu, Ki-Hyun ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 212~219
DOI : 10.5423/PPJ.2004.20.3.212
Three isolates of Cucumber mosaic virus (CMV) from lily plants showing mosaic and distortion symptoms were detected by reverse-transcriptase polymerase chain reaction (RT-PCR) using primers specific to Cucumovirus genus namely, LK-CMV, LK4-CMV, and LKS-CMV. Restriction enzymes patterns of the RT-PCR products revealed that the lily isolates belonged to subgroup IA of CMV. In terms of biological properties, the lily isolates have highly similar but distinct pathogenicity as reported in other lily strains and ordinary strains of CMV. To characterize the molecular properties, cDNAs containing coat protein (CP) gene and 3' non-coding region (NCR) of RNA3 for the isolates were cloned and their nucleotide sequences were determined. The CP similarity (218 amino acids) was highly homologous (>97％) with that of subgroup I CMV strains. However, an additional 20-nulcleotide long segment was only present in 3' NCR of lily isolates, which form an additional stem-loop RNA structure. By using chimeric construct exchange cDNA containing 3'NCR of LK-CMV into the full-length cDNA clone of RNA3 of Fny-CMV, this additional segment may prove to be significant in the identification and fitness of the virus in lily plants. The pathology of zucchini squash infected by F1F2L3-CMV, a pseudorecombinant virus was showed to change drastically the severe mosaic and stunting symptom into a mild chlorotic spot on systemic leave, compared with Fny-CMV. To delimit the sequence of RNA3 affected the pathology, various RNA3 chimeras were constructed between two strains of CMV. The symptom determinants of F1F2L3-CMV were mapped to the positions amino acid 234, 239, and 250 in 3a movement protein (MP). RNA3 chimeras changed the sequences encoding three amino acids were resulted in alteration of systemic symptom.
First Report on Poinsettia mosaic virus in Korea
Chung, B.N. ; Lee, E.K. ; Jeong, M.I. ; Kim, H.R. ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 220~223
DOI : 10.5423/PPJ.2004.20.3.220
Most plants of commercial poinsettia cultivars grown from cuttings develop mosaic and chlorotic dot symptoms on leaves. Reverse transcription-polymerase chain reaction (RT-PCR) test showed that they were infected with Poinsettia mosaic virus (PnMV). In a survey of commercially grown poinsettias conducted in Korea, PnMV was detected in ten of ten poinsettia cultivars sampled and in 100％ of 178 samples tested. The virus has isometric particles and about 29 nm in diameter. Crystalline virus particles were observed in cytoplasm of cells of diseased plants by transmission electron microscopy. Nucleotide sequence of coat protein gene of PnMV- Kl showed 97.3％ homology with that of a German isolate. This is the first report on PnMV in Korea.
First Report on Carnation vein mottle virus in Dianthus barbatus in Korea
Chung, B.N. ; Kim, B.D. ; Choi, G.S. ; Kim, J.S. ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 224~228
DOI : 10.5423/PPJ.2004.20.3.224
A potyvirus causing chlorotic mottle and yellow spots on leaves of Dianthus barbatus was isolated and identified as an isolate of Carnation vein mottle virus (CVMV). Purified preparations of Chenopodium quinoa infected with CVMV-K showed filamentous particles between 695 and 785 om long. Many cytoplasmic inclusions were observed, and these consisted of pinwheels, dense bands, loops, and circles. The coat protein of CVMV-K was about 32 KDa in western blot analysis using a CVMV antibody. The nucleotide sequence of coat protein gene showed 97.6％ homology with a Japanese isolate. The genome size of CVMV-K was about 9.0 kb by dsRNA analysis. These results indicate that the virus is an isolate of CVMV. This is the first report on CVMV in Korea.
Insertional Transposon Mutagenesis of Xanthomonas oryzae pv. oryzae KXO85 by Electroporation
Lee, Byoung-Moo ; Park, Young-Jin ; Park, Dong-Suk ; Kang, Hee-Wan ; Lee, Gil-Bok ; Hahn, Jang-Ho ;
The Plant Pathology Journal, volume 20, issue 3, 2004, Pages 229~233
DOI : 10.5423/PPJ.2004.20.3.229
The bacterial leaf blight, which is caused by Xantho-monas oryzae pv. oryzae, is the most damaging and intractable disease of rice. To identify the genes involved in the virulence mechanism of transposon TnS complex, which possesses a linearized transposon and transposase, was successfully introduced into X. oryzae pv. oryzae by electroporation. The transposon mutants were selected and confirm the presence of transposition in X. oryzae pv. oryzae by the PCR amplification of transposon fragments and the Southern hybridization using these mutants. Furthermore, transposon insertion sites in the mutant bacterial chromosome were deter-mined by direct genomic DNA sequencing using transposon-specific primers with ABI 3100 Genetic Analyzer. Efficiency of transposition was influenced mostly by the competence status of X. oryzae pv. oryzae cells and the conditions of electroporation. These results indicated that the insertion mutagenesis strategy could be applied to define function of uncharacterized genes in X. oryzae pv. oryzae.