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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of The Korean Society of Grassland and Forage Science
Journal Basic Information
Journal DOI :
The Korean Society of Grassland and Forage Science
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Volume & Issues
Volume 26, Issue 4 - Dec 2006
Volume 26, Issue 3 - Sep 2006
Volume 26, Issue 2 - Jun 2006
Volume 26, Issue 1 - Mar 2006
Selecting the target year
Development of High Quality Forage Grass by Down-regulating Lignin Biosynthetic Gene
Woo Hyun-Sook ; Yun Jung-Woo ; Lee Byung-Hyun ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 1~8
DOI : 10.5333/KGFS.2006.26.1.001
To develop a new variety of orchardgrass with improved digestibility, caffeic acid O-methyltransferase (Dgcomt), which is a methylation enzyme involved in the early stages of lignin biosynthesis, was isolated and characterized. Dgcomt was expressed not only in leaves but also in stems and roots. The expression levels of transcripts were high in stems and roots which are the most lignified tissues, and only moderate levels of transcripts were expressed in leaves. To develop transgenic orchardgrass plants by down-regulating the Dgcomt gene, an RNAi suppression vector with partial Dgcomt DNA fragment was constructed and transferred into the genome of orchardgrass via Agrobacterium-mediated gene transfer method. PCR and Southern blot analyses with genomic DNAs from putative transgenic plants revealed that the T-DNA region containing RNAi construct was successfully integrated into the genome of orchardgrass. Northern blot analysis revealed that the majority of the down-regulated transgenic lines showed significant reduction in Dgcomt gene expression. These RNAi transgenic orchardgrass will be useful for molecular breeding of new variety with improved digestibility by down-regulating lignin biosynthetic enzyme.
A Cold-Tolerant and Early-Heading Italian Ryegrass New Variety, 'Kogreen'
Choi Gi-Jun ; Lim Young-Chul ; Rim Yong-Woo ; Sung Byung-Ryul ; Kim Meing-Jooung ; Kim Ki-Yong ; Seo Sung ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 9~14
DOI : 10.5333/KGFS.2006.26.1.009
This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) at the Grassland and Forage Crops Division, National Livestock Research Institute, RDA at Suwon, Korea, from 1999 to 2005. The new variety 'Kogreen' is a diploid variety with a green leaf color and had semi-erect habit in late autumn and erect growth habit in early spring. The heading date of 'Kogreen' was on May 4, and for 'Florida 80' was May 2, both are considered as early-heading variety. 'Kogreen' was wider by 0.7mm in flag leaf width, longer by 2 cm in flag leaf length and shorter by 1cm in plant length than the control variety, Florida 80. Stem width of 'Kogreen' was similar to that of 'Florida 80' and 'Hwasan 101'. cold tolerance of 'Kogreen' was better than that of 'Florida 80'. Dry matter(DM) yield (12,884kg/ha) of 'Kogreen' was 3% less than that of 'Florida 80'. in vitro dry matter digestibility was 74.7% and total digestible nutrient was 65.1%, which are 3.6% and 1.5% higher than those of 'Florida 80', respectively. Acid detergent fiber and neutral detergent fiber of 'Kogreen' were 30.1% and 54.1% which are 1.9% and 1.3% lower than those of 'Florida 80', respectively.
Determination of Nutrient Contents of Liquid Pig Manure and the Correlation of Components as Fertilizer in Western JeJu Area
Song Sang-Taek ; Kim Mun-Chol ; Hwang Kyoung-Jun ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 15~24
DOI : 10.5333/KGFS.2006.26.1.015
This study was conducted to make a rapid and easy determination for the fertility of liquid pig manure as fertilizer by investigating the contents, and correlation coefficients of various nutrients. Samples were collected from 118 local pig farms in the western area of Jeju in Korea. Electrical conductivity(EC), dry matter(DM),
and minerals were determined and the relationships among them were examined. The collected liquid manure samples from 118 pig farms were classified according to the level of DM contents ;< 3% (92 farms),
(5 farms) and>9% (3 farms), based on the collected data, most of the liquid manure coming from the local pig farms contain small amount of dry matter. The dry matter contents appeared highly correlated(p<0.01) with EC,
, T-P, Ca, Mg and Na, except for K. In addition EC was proportional to
, T-P, Ca, and Na except fer Mg. The fertilizer component ratio of
, P and K in liquid pig manure were not constant, resulting in low efficiency for fertilizer. However, the toxic heavy metals of Cu etc. were below the criteria of organic fertilizer and soil contamination evaluation. Therefore, we concluded that both dry matter content and electrical conductivity could be used as an indicator for evaluating the fertility of liquid pig manure.
Effects of Urea and Ammonium Sulfate Application on Yield and Nutritive Value of Whole Crop Barley in Reclaimed Tideland
Shin Jae-Soon ; Kim Won-Ho ; Lee Seung-Heon ; Lim Young-Chul ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 25~30
DOI : 10.5333/KGFS.2006.26.1.025
This experiment was conducted to compare the fresh yield and dry matter yield, and nutritive values of whole crop barley treated with Urea (200 kg/ha, T2) and ammonium sulfate fertilizer as 200 kg/ha (T3), 300 kg/ha (T4) and 400 kg/ha (T5) at the Bae-Ho reclaimed tideland. Korea from 2003 to 2005. Salt contents of soil in the ammonium sulfate plots (T3, T4, T5) were lower than those of T2. The fresh yields of ammonium sulfate plots (T3, T4, T5) were higher than those of T2 as 62% (p>0.05), 41% (p>0.05) and 23% (p<0.05), respectively. The dry matter yield of T3, T4 and T5 (ammonium sulfate) was significantly (p<0.05) higher at 5,080 kg/ha, 4,667 ka/ha, 4,040 kg/ha, respectively. The total digestible nutrients (TDN) yield tends to have a similar result. Crude protein (CP) content was highest in T3 and CP trends to decrease as the level of ammonium sulfate was increased. Total digestible nutrient (TDN) were high in T3 and T4. The sodium content of T3 and T4 were lower than T2. Based on the study, it was more desirable to use ammonium sulfate (200 kg N/ha) rather than Urea as fertilizer on reclaimed land in terms of forage production and nutritive value.
Forage Performance Evaluation of Rye Cultivars with Different Sowing Dates
Kim Su-Gon ; Kim Jong-Duk ; Kwon Chan-Ho ; Ha Jong-Kyu ; Kim Dong-Am ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 31~38
DOI : 10.5333/KGFS.2006.26.1.031
This experiment was carried out to determine the effects of different sowing dates and cultivars on the forage yield and quality of winter rye (Secale cereale L.). The experiment was conducted in a split plot design with three replications. The main plots consisted of two sowing dates such as early (15 September) and late (30 September). The subplots consisted of rye cultivars of five different maturity groups such as 'Kodiak' (Late), 'Koolgrazer' (Early), 'Danko' (Late), 'Homil22' (Medium) and 'Olhomi1' (Early). Dry matter (DM) content at early sowing (19.7%) was higher than those at late sowing (17.8%), while crude protein (CP) and total digestible nutrients (TDN) contents at early sowing (16.6% and 61.6%) were lower than those at late sowing (16.8% and 63.1%). Although DM content of early maturing cultivars (Koolgrazer and Olhomi1) was higher than the other cultivars, CP and TDN contents of those were lower compared to other cultivars. The contents of fiber components (ADF, NDF and cellulose) at early sowing were higher than those at late sowing, while ADL and hemicellulose at early sowing were lower than those at late sowing. ADF and cellulose of early maturing cultivars were higher than those of the other cultivars, while ADL and hemicellulose of Danko were higher than that of the other cultivars. DM, CP, In vitro dry matter digestibility (IVDMD) and TDN yields at early sowing were higher than those at late sowing, and early maturing cultivars were higher than those of the other cultivars. Results of this study indicate that forage production technology in combination with early sowing and use of early maturing rye cultivar could enhance both production and quality of rye.
Effects of Wilting Days on the Quality of Round Baled Grass Silage
Kim Jong-Geun ; Chung Eui-Soo ; Seo Sung ; Ham Jun-Sang ; Kim Maeong-Joong ; Lee Jong-Kyeong ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 39~44
DOI : 10.5333/KGFS.2006.26.1.039
This experiment was conducted to determine the effect of management practices on the qualify of round baled grass silage at experimental field of grassland and Forage Crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consisted of three different wilting days : 0 (unwitted), 0.5 and 1 day with three replications. Crude fiber content was increased with prolonged wilting period, but other components did not show any trends. There was no significant differences in acid detergent fiber (ADF) and neutral detergent fiber (NDF) content between wilting periods, but there was significant difference in IVDMD (in vitro dry matter digestibility) between one-day wilted and unwitted silage. Dry matter (DM) content changed from 23.7% to 40.5% in relation to prolonged wilting period. Wilting treatment caused a decrease in each organic acid content of round baled grass silage and an increase in the silage quality. Wilting also could reduce the DM loss. This study demonstrated that one-day wilting would be a recommendable method for making high quality round baled grass silage.
Prediction of the Digestibility and Energy Value of Corn Silage by Near Infrared Reflectance Spectroscopy
Park Hyung-Soo ; Lee Jong-Kyung ; Lee Hyo-Won ; Kim Su-Gon ; Ha Jong-Kyu ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 45~52
DOI : 10.5333/KGFS.2006.26.1.045
This study was carried out to explore the accuracy of Near Infrared Reflectance Spectroscopy (NIRS) fer the prediction of digestibility and energy value of corn silages. The spectral data were regressed against a range of digestibility and energy parameters using modified partial least squares(MPLS) multivariate analysis in conjunction with first and second order derivatization, with scatter correction procedure(SNV-Detrend) to reduce the effect of extraneous noise. Calibration models for NIRS measurements gave multivariate correlation coefficients of determination
and standard errors of cross validation of 0.92(SECV 1.73), 0.91(SECV 1.13) and 0.93(SECV 1.74) for in vitro dry matter digestibility(IVDMD), in vitro true digestibility(IVTD), and cellulase dry matter digestibility(CDMD), respectively. The standard error of prediction(SEP) and the multiple correlation coefficient of validation
on the validation set(n=39) was used in comparing the prediction accuracy. The SEP value was 0.30(TDN), 0.01(NEL), and 0.01(ME). The relative ability of NIRS to predict digestibility and energy value was very good for CDMD, total digestible nutrients(TDN), net energy fer lactation(NEL) and metabolizable energy(ME). This paper shows the potential of NIRS to predict the digestibility and energy value of con silage as a routine method in feeding programmes and for giving advice to farmers.
Effect of Sample Preparations on Prediction of Chemical Composition for Corn Silage by Near Infrared Reflectance Spectroscopy
Park Hyung-Soo ; Lee Jong-Kyung ; Lee Hyo-Won ; Hwang Kyung-Jun ; Jung Ha-Yeon ; Ko Moon-Suck ;
Journal of The Korean Society of Grassland and Forage Science, volume 26, issue 1, 2006, Pages 53~62
DOI : 10.5333/KGFS.2006.26.1.053
Near infrared reflectance spectroscopy (NIRS) has been increasingly used as a rapid, accurate method of evaluating some chemical compositions in forages. Analysis of forage quality by NIRS usually involves dry ground samples. Costs might be reduced if samples could be analyzed without drying or grinding. The objective of this study was to investigate effect of sample preparations and spectral math treatments on prediction ability of chemical composition for corn silage by NIRS. A population of 112 corn silage representing a wide range in chemical parameters were used in this investigation. Samples of com silage were scanned at 2nm intervals over the wavelength range 400-2500nm and the optical data recorded as log l/Reflectance(log l/R) and scanned in overt-dried grinding(ODG), liquid nitrogen grinding(LNG) or intact fresh(IF) condition. Samples were analysed for neutral detergent fiber(NDF), acid detergent fiber(ADF), acid detergent lignin(ADL), crude protein(CP) and crude ash content were expressed on a dry-matter(DM) basis. The spectral data were regressed against a range of chemical parameters using modified partial least squares(MPLS) multivariate analysis in conjunction with four spectral math treatments to reduce the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation(SECV). The results of this study show that NIRS predicted the chemical parameters with very high degree of accuracy(the correlation coefficient of cross validation
) in ODG. The optimum equations were selected on the basis of minimizing the standard error of prediction(SEP). The Optimum sample preparation methods and spectral math treatment were for ADF, the ODG method using 2,10,5 math treatment(SEP = 0.99,
), and for CP, the ODG method using 1,4,4 math treatment(SEP = 0.29.