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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of The Korean Society of Grassland and Forage Science
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Journal DOI :
The Korean Society of Grassland and Forage Science
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Volume & Issues
Volume 31, Issue 4 - Dec 2011
Volume 31, Issue 3 - Sep 2011
Volume 31, Issue 2 - Jun 2011
Volume 31, Issue 1 - Mar 2011
Selecting the target year
Factors Influencing Efficient Agrobacterium-mediated Transformation of Panicum spp.
Seo, Mi-Suk ; Takahara, Manabu ; Takamizo, Tadashi ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 1~8
DOI : 10.5333/KGFS.2011.31.1.1
Molecular techniques such as genetic transformation are powerful tools that can be used for the genetic modification of warm-season grasses. The P. meyerianum with high regeneration ability was used for establishing an Agrobacterium-mediated transformation system. We investigated various factors affecting Agrobacterium infection by examining GUS gene expression of pCAMBIA1304 vector. Among various concentration of acetosyringone and betaine tested for inoculation and co-cultivation, 10 mg/L acetosyringone and 60 mg/L betaine resulted in the highest transformation frequency in terms of GUS expression. The calli of 4 species of Panicum spp. with excellent tissue culture response were inoculated with Agrobacterium under the optimal infection conditions. The high activity of GUS gene was observed in all species and hygromycin-resistant calli expressing GFP were obtained in P. meyerianum, P. longijubatum, P. stapfianum and guineagrass Noh-PL1. Co-cultivated calli were transferred onto the selection medium containing hygromycin, and the hygromycin resistant calli were selected after 3 months. Hygromycin-resistant plantlets were then successfully regenerated from the calli and grown in a greenhouse. We confirmed stable insertion of hpt gene among the hygromycin-resistant plantlets of P. meyerianum by PCR analysis.
A Very Early-Maturing Italian Ryegrass (Lolium multiflorum Lam.) New Variety, `Green Farm` for Double Cropping System
Ji, Hee-Chung ; Lee, Sang-Hyun ; Yoon, Sei-Hyung ; Kim, Ki-Yong ; Choi, Gi-Jun ; Park, Hyung-Soo ; Park, Nam-Gun ; Lim, Young-Chul ; Lee, Eun-Sup ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 9~14
DOI : 10.5333/KGFS.2011.31.1.9
This experiment was carried out to breed a very early maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 2009 to 2010. A new variety, `Green farm` is a diploid variety with green in leaf color and has erect and semi-erect growth habit in late autumn and early spring, respectively. `Green farm` was on 28 April in heading date as a early-maturing variety. `Green farm` was also wider by 0.4 mm in flag leaf width, longer by 0.8 cm in flag leaf length and shorter by 5 cm in plant height than those of control variety, `Florida 80`, respectively. `Green farm` was thicker in stem thickness and stronger in winter hardness than those of the `Florida 80`. Dry matter (DM) yield (11,790 kg/ha) of `Green farm` was similar to that of `Florida 80`. In vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN) and crude protein (CP) of `Green farm` were 68.7, 63.3 and 10.3% which are 1.9, 1.7 and 0.6% higher than those of `Florida 80`, respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of `Green farm` were 32.5 and 54.6% which are 2.2 and 4.3% lower than those of the `Florida 80`, respectively.
Glutathione Concentration as Affected by Sulfate Supply Level and its Relationship with Sulfate Uptake and Assimilatory Enzymes Activity in Rape Plants
Li, Lu-Shen ; Kim, Tae-Hwan ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 15~24
DOI : 10.5333/KGFS.2011.31.1.15
The glutathione (GSH) concentration in leaves of different maturities and roots of forage rape (Brassica napus L.) supplied with four levels of external
(0, 0.1, 1.0 and 2.0 mM) supply were measured. The relationships of GSH concentration with
uptake, ATP sulfurylase (ATPS) and O-acteylserine (thiol) lyase (OASTL) activity were also assessed. The
uptake increased in parallel with the external
supply, while protein concentration was not significantly changed. The ATPS activity increased continuously with decreasing
supply from 2.0 to 0 mM, while the OASTL activity decreased significantly only at S-deficient conditions (0 and 0.1 mM). The GSH concentration in the young leaves, middle leaves and roots continuously increased (except for between 1.0 and 2.0 mM in the middle leaves and roots) as the external S supply was increased, but no significant changes occurred in the old leaves. The increased endogenous GSH concentration, affected by the
supply level, was significantly related with the decrease in ATPS activity in both leaves and roots, and the decrease in OASTL activity only in leaves..
Comparisons of Characteristics, Yield and Feed Quality of Oat Varieties Sown in Spring and Autumn
Ju, Jung-Il ; Lee, Dong-Hee ; Han, Ouk-Kyu ; Song, Tae-Hwa ; Kim, Chang-Ho ; Lee, Hee-Bong ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 25~32
DOI : 10.5333/KGFS.2011.31.1.25
Oats are an important crop for forage production with good palatability of livestock. Compared with other winter cereals crops for forage, oats may be widely sowing, autumn (October), spring (early of March) and summer (late of August). The objectives of this study were to compare the effects of sowing dates and varieties on the growth, forage yield and feed quality. Oat were sown in 14 October and 10 March, and harvested 20 days after heading in middle region of Korea. Varieties used were the 3 winter oat cultivars with cold tolerant and 3 cultivars for summer sowing. Heading was delayed about 12 days in spring sowing than in autumn sowing and the difference among varieties was 8 days. The rate of spike and leaf above aerial parts reduced and the rate of culm increased in spring sowing than in autumn. The variety `Donghan` was higher the rate of spike and lower the rate of culm than that of other varieties. In spring sowing than in autumn, acid detergent fiber (ADF) content was higher, and neutral detergent fiber (NDF) and digestible dry matter (DDM) content was lower. In DDM content surveyed on 20 days after heading, `Donghan` was higher in autumn sowing and `Samhan` in spring sowing than that of other varieties. The oat variety `Samhan`, `Donghan` and `Chohan` were higher fresh yield in autumn than in spring, but similar dry matter yield. The variety `Swan`, `Darkhorse` and `Hispeed` were higher fresh and dry matter yield in spring than in autumn. The variety `Donghan` can supplement high quality forage production in middle region at October and March because of the high-tillering and rate of spike per aerial part.
Dry Matter Yield and Forage Quality at Mixture of Annual Legumes and Italian ryegrass on Paddy Field
Kim, Won-Ho ; Kim, Ki-Young ; Jung, Min-Woong ; Ji, Hee-Chung ; Lim, Young-Chul ; Seo, Sung ; Kim, Jong-Duk ; Yoon, Bong-Ki ; Lee, Hyo-Won ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 33~38
DOI : 10.5333/KGFS.2011.31.1.33
This experiment was conducted to compare the dry matter yield and forage quality according to the seed mixture of annual legumes and Italian ryegrass (Lolium multiflorum, IRG) on paddy field at the experimental field of Jeollanam-Do Agricultural Research and Extension Services from 2007 to 2008. The five treatments used in this experiment were IRG single (cv. Kowinearly), IRG+Chinese milk vetch mixture, IRG + Crimson clover (cv. Linkarus) mixture, IRG + Austrian winter pea (Pisum sativum subsp. arvense cv. forage Pea) mixture and IRG + Hairy vetch (Vicia villosa. cv. Oregon Common) mixture. And the mixing ratio of Italian ryegrass + annual legumes were 70 : 30 as based mono seed rate. The dry matter (DM) percentage at harvest was 23.6~26.8%. The DM percentage among IRG single and mixture treatments were similar. The yields of fresh and CP were high in IRG + Forage pea mixtures as a 40,100 kg and 625 kg per ha, respectively (p<0.05). The yields of dry matter were high in IRG + Forage pea and IRG + hairy vetch pea mixtures as a 9,470 kg and 9,500 kg per ha, respectively (p<0.05). But the forage quality did not show difference between Italian ryegrass mono-culture and annual legumes mixture. The Av.
in IRG+Forage pea mixture was 78 mg/kg and concentration of K were 0.52~0.88. In conclusion, the mixture of IRG and Forage pea, dry matter yield, protein yield increased, and was effective in enhancing the stability of cultivation.
Effect of Early Harvest on the Forage Yield, Quality and Regrowth of Italian Ryegrass and Barley Sown in Early Spring
Seo, Sung ; Kim, Won-Ho ; Kim, Ki-Yong ; Jung, Min-Woong ; Choi, Gi-Jun ; Park, Hyung-Soo ; Lee, Joung-Kyong ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 39~46
DOI : 10.5333/KGFS.2011.31.1.39
This study was carried out to determine the forage yield, quality, and regrowth of Italian ryegrass (IRG) and barley sown on 2 March 2009 in Suwon. The five treatments were two IRG cultivars (Kowinearly with early maturity and Kowinmaster with medium maturity), one barley cultivar (Yuyeon), and two mixtures (Kowinearly + Yuyeon and Kowinmaster + Yuyeon). The first harvest date was 26 May, which was at late heading, heading and early dough stage of Kowinearly, Kowinmaster and barley, respectively. Regrowth yield was investigated on 29 June. The heading dates of Kowinearly and Kowinmaster were 16 May and 22 May, respectively, and that of barley was 13 May. The dry matter (DM) percentage were 13.0~18.4% at first harvest, and 22.5~24.8% at regrowth in all treatments. The forage yield of barley and Kowinmaster + Yuyeon mixture at first harvest was higher than that of IRG (p<0.05), but higher regrowth yield was observed in IRG, and then IRG + barley mixtures (p<0.05). The crude protein (CP) content and in vitro DM digestibility (IVDMD) of IRG at first harvest were 16.7~17.1% and 78.3~80.4%, respectively, which were higher than those of barley (CP 12.2% and IVDMD 72.6%) and IRG + barley mixtures. The total yields of DM, CP and digestible DM were high in Kowinmaster + Yuyeon mixture as 11,628 kg, 1,669 kg and 8,457 kg per ha, respectively. In conclusion, spring seeding of IRG + barley mixtures and/or barley were recommended when early harvest. Regrowth of IRG sown in early spring was vigorous. Mixture cultivation of IRG and barley was effective, because of forage yield and stable production, and harvest at June instead of May was desirable for forage productivity of spring sown IRG and barley.
Effect of Drought Conditions on Growth, Forage Production and Quality of Silage Corn at Paddy Field
Ji, Hee-Chung ; Cho, Jung-Ho ; Lee, Sang-Hoon ; Kim, Won-Ho ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 47~54
DOI : 10.5333/KGFS.2011.31.1.47
This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field of Jeonnam and Cheonan region. The growth, forage production and quality of silage corn in normal condition showed much better than drought condition at paddy field. Among the growth characteristics, `Kangdaok` hybrids was somewhat strong for drought stress, then and good at stem diameter, drought stress, sugar content, stay green, disease and insect resistance. Fresh yield of `Kwangpyongok` and `Kangdaok` hybrid at drought paddy field were the highest as 9,714kg and 9,126 kg/ha per ha among corn hybrids. Among the ten hybrids, dry yield of `Kangdaok` hybrid at drought paddy field was the highest as 5,548 kg per ha. The result of this study showed that `Kangdaok` hybrid had good growth characters and forage productivity at drought condition and dry matter yield and TDN yield also were 21.6% and 19.3% level compared with normal paddy field.
Effects of Forage Cropping System and Cattle Slurry Application on Productivity of Rice and Forage Crops and Nutrient Movement in Paddy Land
Yook, Wan-Bang ; Choi, Ki-Choon ; Lee, Kyeong-Bo ; Kang, Yo-Sep ; Yoon, Chang ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 55~64
DOI : 10.5333/KGFS.2011.31.1.55
This study was conducted to investigate the effects of green manure crops and cattle slurry application on rice productivity and nutrient movement in paddy land. Cropping systems used in this study were consisted of five designs, such as mono-cropping rice applied with standard fertilizer (MRS), double-cropping whole crop barley following rice applied with cattle slurry (DWBRC), double-cropping whole crop rye following rice applied with cattle slurry (DWRRC), mono-cropping rice applied with following milk vetch (MRMV) and mono-cropping rice applied with following hairy vetch (MRHV). The field experiments were conducted on the clay loam at Backsanmyun, Kimje, Chunlabukdo province in Korea for three years (May 2006 to Apr. 2009). This study was arranged in completely randomized design with three replicates. Culm and panicle length of rice were higher in MRS than other treatments. Ripened grain ratio was higher in MRS than other treatments. Yield of Grain showed high in MRS, DWBRC and DWRRC than MRMV and MRHV. The yield of dry matter (DM) of whole crop barley in DWBRC increased significantly as compared with that of rye in DWRRC. The pH, and contents of T-N,
and organic matter (OM) and exchangeable cation (Ca, Na, Mg and K) in soil samples collected at the end of the experiment were remarkably higher than those at the beginning of the experiment. The concentrations of
in discharge water in DWBRC and DWRRC were higher than MRS.
Effect of Harvest Stage of Corn on Nutritive Values and Quality of Roll Baled Corn Silage Manufactured with Corn Grown in Paddy Land
Choi, Ki-Choon ; Jo, Nam-Chul ; Jung, Min-Woong ; Lee, Kyung-Dong ; Kim, Jong-Geun ; Lim, Young-Chul ; Kim, Won-Ho ; Oh, Yung-Keun ; Choi, Jin-Hyuk ; Kim, Cheon-Man ; Jung, Du-Keun ; Choi, Jong-Man ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 65~74
DOI : 10.5333/KGFS.2011.31.1.65
This study was carried out to examine the effect of harvest stage of corn on nutritive values and quality of round baled corn silage manufactured with corn grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA from 2009 to 2010. Corn "Kwangpyungok" was harvested at three different growth times (milk, yellow ripen and ripen stage) and ensiled at each harvest stages. Crude protein (CP) and TDN (total digestible nutrient) contents of round baled corn silage was decreased and in vitro dry matter digestibility (IVDMD) was not changed with delayed harvest maturity. However, contents of ADF (acid detergent fiber) and NDF(neutral detergent fiber) decreased with delayed harvest maturity. The pH at three different harvest stages ranged from 3.8 to 4.0. The content of lactic acid increased with delayed harvest maturity, but the content of acetic acid decreased. And then, flieg`s score reveals that there is an increase in order, ripen stage > yellow ripen stage > milk stage. Therefore, this study suggest that round baled corn silage manufactured at yellow ripen stage can improve the silage quality.
Study on Manufacture of Square Baled Corn Silage Using Square Silage Wrapping Compressor
Choi, Ki-Choon ; Cho, Nam-Chul ; Jung, Min-Woong ; Kim, Jong-Geun ; Shin, Jae-Soon ; Lee, Kyung-Dong ; Lim, Young-Chul ; Kim, Won-Ho ; Oh, Young-Keun ; Kim, Cheon-Man ; Kim, Hyuk-Gi ; Han, Dong-Bae ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 1, 2011, Pages 75~84
DOI : 10.5333/KGFS.2011.31.1.75
This study was conducted to investigate the effect of harvest stage of corn on the quality of square baled corn silage manufactured with corn grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA from 2009 to 2010. Corn "Kwangpyungok" was harvested at three different growth stages (milk, yellow ripen and ripen stage) and ensiled at each harvest time. Square baled corn silage was manufactured by use of square silage wrapping compressor. Each treatment was replicated three times. The content of crude protein (CP) of corn in square baled corn silage decreased with delayed maturity, but the content of ADF (acid detergent fiber), NDF (neutral detergent fiber), TDN (total digestible nutrient) and in vitro dry matter digestibility (IVDMD) were not changed. The content of moisture, pH and the nutritive values at three different harvest stages were not influenced by the method of silage manufacture and inoculant. The content of lactate of square baled corn silage harvested in milk stage of corn was significantly increased, as compared with that of round baled corn silage (P<0.05), but in stage of yellow ripen was significantly decreased (P<0.05). The content of acetate in square baled corn silage significantly decreased with delayed harvest maturity, as compared with that of round baled corn silage (P<0.05). Flieg`s score of square baled corn silage harvested in milk stage of corn was slightly higher than that of round baled corn silage, but Flieg`s scores in yellow ripen stage and ripen stage were not influenced by the method of silage manufacture. Flieg`s score with delayed maturity was not influenced by the method of silage manufacture and inoculant. The manufacture of square baled corn silage was proved to be suitable for the fermentation of corn silage. Therefore, this study suggest that square baled corn silage can be a way of new silage manufacture technique.