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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of The Korean Society of Grassland and Forage Science
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Journal DOI :
The Korean Society of Grassland and Forage Science
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Volume & Issues
Volume 31, Issue 4 - Dec 2011
Volume 31, Issue 3 - Sep 2011
Volume 31, Issue 2 - Jun 2011
Volume 31, Issue 1 - Mar 2011
Selecting the target year
Plant Regeneration from Seed Derived Callus of four Cultivars of Timothy
Lee, Ki-Won ; Choi, Gi-Jun ; Kim, Ki-Yong ; Ji, Hee-Chung ; Jung, Min-Wong ; Kim, Kyung-Hee ; Lee, Byung-Hyun ; Lee, Sang-Hoon ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 211~216
DOI : 10.5333/KGFS.2011.31.3.211
The present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature seed derived callus of four cultivars of Timothy. In order to investigate the effects of genetic variations of timothy in tissue culture, calli were induced from mature seeds of four varieties, `Colt`, `Chair`, `Richmond` and `Hokuo` and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype `Colt` consistently performed best in the callus subculture and plant regeneration. The complete plantlets were thereafter transplanted to grow under greenhouse condition. Regenerated timothy plants were morphologically uniform with normal leaf and growth pattern.
In Vitro Plant Regeneration of Siberian Wildrye Grass from Mature Seed-derived Callus
Lee, Ki-Won ; Chinzorig, Ochirbat ; Choi, Gi-Jun ; Yoon, Sei-Hyung ; Kim, Ki-Yong ; Ji, Hee-Chung ; Lee, Sang-Hoon ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 217~222
DOI : 10.5333/KGFS.2011.31.3.217
Success in molecular breeding for better adapted varieties to environmental stresses depend upon the concerted efforts by various research including tissue culture, transformation, genetics and breeding. In order to optimize tissue culture conditions of Siberian wildrye grass, the effects of plant growth regulators on callus induction and plant regeneration were investigated with mature seeds. The highest callus induction frequency was observed when the mature seeds were cultured on MS medium supplemented with 5 mg/L 2,4-D. The highest plant regeneration frequency was observed when callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of Siberian wildrye grass by the production of transgenic plant.
Plant Regeneration from Mature Seed-Derived Callus in Bermudagrass (Cynodon dactylon)
Lee, Ki-Won ; Park, Hyung-Soo ; Choi, Gi-Jun ; Kim, Ki-Yong ; Ji, Hee-Chung ; Kim, Kyung-Hee ; Lee, Byung-Hyun ; Lee, Sang-Hoon ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 223~228
DOI : 10.5333/KGFS.2011.31.3.223
The present study was conducted to determine the optimum in vitro culture condition for callus induction and plant regeneration from mature seeds of bermudagrass (Cynodon dactylon cv. Common). It was revealed that mature seeds cultured on MS medium supplemented with 2 mg/L 2,4-D, 0.5 g/L proline, 0.5 g/L casamino acid and 3 g/L Gelrite under light condition produced the highest percentage of callus formation (39.2%). The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 0.5 mg/L 2,4-D, 2 mg/L BA, 0.5 g/L proline, 0.5 g/L casamino acid 3 g/L Gelrite which induced the highest percentage of calli forming shoots (57.7%). The frequency of callus induction and plant regeneration were the highest on sucrose, followed by maltose. The shoots were rooted at the highest rate (100%) when transferred onto 1/2 MS medium. Regenerated plants were morphologically uniform with normal growth pattern.
Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Seed Culture of Reed
Kim, Yong-Goo ; Kim, Kyung-Hee ; Lee, Byung-Hyun ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 229~234
DOI : 10.5333/KGFS.2011.31.3.229
In order to develop an efficient, reliable and reproducible tissue culture system for reed (Phragmites communis Trinius), an efficient plant regeneration system via callus induction was established using mature seeds as explants. MS medium containing 1 mg/L 2,4-D and 0.5 mg/L BA was optimal for callus induction from mature seeds. The highest frequency (88.7%) of callus formation was obtained in 1.0 mg/L 2,4-D. The highest plant regeneration frequency (59.6%) was found when the embryogenic calli were subcultured on MS medium supplemented with 100 mg/L myo-inositol, whereas, adding of plant growth regulators was not so promising in this case. Our result would be useful for development of transgenic reed plants.
Plant Regeneration From Mature Seed of Domestic Italian Ryegrass Cultivar
Kim, Kyung-Hee ; Kim, Yong-Goo ; Heo, Sung-Hyun ; Lee, Ki-Won ; Lee, Byung-Hyun ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 235~242
DOI : 10.5333/KGFS.2011.31.3.235
In order to improve forage characteristics of Italian ryegrass by genetic transformation, an efficient callus induction from mature seed and optimal plant regeneration system were established using a domestic cultivar `Kospeed`. Addition of 5 mg/L of 2,4-D showed highest frequency of embryogenic callus induction from mature seeds. N6 medium showed higher frequency of both callus induction and plant regeneration as compared with MS and SH medium. The highest plant regeneration frequency 67% was obtained when embryogenic calli were transferred to N6 medium containing 1 mg/L 2,4-D and 5 mg/L BA. Supplementation of regeneration medium with sucrose at 30 g/L level maximized regeneration frequency as compared to the other concentrations. These data would be very helpful for molecular breeding of domestic Italian ryegrass cultivar through genetic transformation.
Performance Trial for Developing High - Yielding and Early Maturing Hairy Vetch (Vicia villosa Roth) Cultivar under Double Cropping System
Shin, Chung-Nam ; Ko, Ki-Hwan ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 243~250
DOI : 10.5333/KGFS.2011.31.3.243
This experiment was conducted to evaluate agronomic characteristics, forage quality and dry matter (DM) yield of early-maturing hairy vetch `Cold green`, `Haymaker plus`, `Cappello` and medium-late maturing cultivar `Cheong pa`, `Challenger`, `Hungvillosa` and `Latigo` at Seongju in the Kyeongbuk from 2009 to 2011. The experiment was arranged in randomized complete block design with three replications. Hairy vetchs were seeded on Sep. 26 and Oct. 11 in 2009 and 2010. Vetch plants were harvested on 30 Apr. in 2010 and 2011. Cold tolerance for Haymaker plus and Cappello were a little lower than other cultivars. Fifty percent-flowering dates of Cold green had earlier than others and Haymaker plus and Cappello were earlier than Cheong pa, Challenger, Hungvillosa and Latigo. Cheong pa and Challenger were ealirer than Hungvillosa and Latigo. The DM yield of Cold green was the highest (p<0.05) of the cultivar except Haymaker plus. Haymaker plus was higher (p<0.05) than others and Cappello, Cheong pa, Challenger were higher (p<0.05) than Hungvillosa and Latigo in 2010. In 2011, the DM yield of Cold green was the highest (p<0.05) of the cultivar and Haymaker plus was higher (p<0.05) than others. Cappello was higher (p<0.05) than Hungvillosa and Latigo. Cheong pa and Challenger were higher (p<0.05) than Latigo. Vetch cultivar was high in crude protein (CP) and low in Acid detergent fiber (ADF). According to the results obtained from this study, it is suggested that early-maturing cultivar would be recommendable for fall sown vetch for DM production.
Response of Growth Characteristics and Yield of Silage Corns at Different Regions
Son, Beom-Young ; Kim, Jung-Tae ; Lee, Jin-Seok ; Baek, Seong-Bum ; Kim, Wook-Han ; Kim, Jong-Duk ; Ko, Ki-Hwan ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 251~260
DOI : 10.5333/KGFS.2011.31.3.251
This study was carried out to compare growth characteristics and yield of silage corn hybrids at four different regions (Hwaseong, Dangjin, Gochang and Pyeongchang). Two domestic corn hybrids, `Kwangpyeongok` and `Gangdaok`, and two imported corn hybrids, `P3394` and `32P75` were used in this study. Plant height and ear height ratio were not significantly different among corn hybrids. `Kwangpyeongok` was the strongest of all corn hybrids for lodging. The others except `Kwangpyeongok` were similar to lodging. All corn hybrids were excellent to stay-green. Incidence of southern corn leaf blight (Bipolaris maydis) was low at all corn hybrids. `32P75` was 10.1%, the most of all corn hybrids for incidence of BSDV (black streaked dwarf virus). `Kwangpyeongok` and `Gangdaok` were stronger than `32P75` for BSDV. Fresh yield of `Kwangpyeongok` and `Gangdaok` were the most of all corn hybrids as 74.85 and 76.68 ton/ha and `P3394` was the least of all corn hybrids as 64.97 ton/ha. Dry matter yield as 18.83~21.92ton/ha and TDN yield as 12.73~14.52 ton/ha were not significantly different among corn hybrids.
Forage Productivity and Quality of Domestic Italian Ryegrass and Barley Varieties
Seo, Sung ; Kim, Won-Ho ; Kim, Ki-Yong ; Choi, Gi-Jun ; Ji, Hee-Chung ; Lee, Sang-Hoon ; Lee, Ki-Won ; Kim, Meing-Jooung ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 261~268
DOI : 10.5333/KGFS.2011.31.3.261
This study was carried out to determine the forage production and quality of Italian ryegrass (IRG) and forage barley developed by Korea in Suwon, 2009~`10. The nine treatments were two IRG varieties (Kowinearly with early maturity and Kowinmaster with medium maturity), five barley varieties (Youngyang, Wooho, Yuyeon, Dami and Youho), and two mixtures (Kowinearly + Yuyeon and Kowinmaster + Yuyeon). The heading dates of Kowinearly and Kowinmaster were 14 May and 18 May, respectively. The growth stage of barley investigated at 22 May were late milk in Youngyang and Wooho, early dough in Dami and early to medium dough in Yuyeon and Youho. Plant length of IRG in IRG + barley mixtures was 117~118 cm, which was longer than those of IRG monoculture of 98~101 cm, and no lodging was found in mixtures. The dry matter (DM) percentage at harvest was 20.7~25.4% in all treatments. The botanical composition of IRG in mixtures was 43.1%. The percentage of spike per barley plant was become high according to progressed maturity, as a 35.7%, 44.1%, 54.8% and 57.2% in late milk, dough, yellowish and full ripeness stage, respectively, and the spike percentages of Youngyang and Wooho were tends to high. The crude protein (CP) content of IRG as 9.0~10.0% was higher than that of barley (7.0~8.5%), and the contents of NDF and ADF of barley were lower than those of IRG, and in vitro DM digestibility were 64.4% in Kowinearly, 64.1% in Kowinmaster, 64.5% in mixture, and 60.2% (Youho) to 66.4% (Wooho) in barley. The yields of DM, CP and in vitro digestible DM were high in Kowinmaster+barley mixture as a 11,508 kg, 1,046 kg and 7,422 kg per ha, respectively (p<0.05). However, no significant differences in forage yield were observed among cultivar of IRG, and barley, although Wooho was tends to high in digestibility and forage yield among five barley varieties. In conclusion, the mixture cultivation of IRG Kowinmaster + forage barley was recommended, because of preventing of IRG lodging, higher plant length of IRG, increasing of forage yield, and stable production. Selection of suitable winter forage species and variety for district, climate environment, and utilization type of farm was also important.
Mycorrhizal Colonization Effects on Some Physiological Parameters Related to Drought Stress in White Clover
Park, Sang-Hyun ; Zhang, Qian ; Kim, Tae-Hwan ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 269~276
DOI : 10.5333/KGFS.2011.31.3.269
To investigate the physiological effects of mycorrhizal colonization on drought stress tolerance in white clover, the responses of leaf water potential (
), relative water content (RWC), leaf dry mass, photosynthesis rate, transpiration, stomatal conductance, proline and ammonia were assessed periodically during 7 days in non-AM and AM plants under wellwatered or drought-stressed conditions. Under well-watered conditions, the examined parameters were not significantly changed or very little affected by AM symbiosis. Drought decreased water potential, relative water content, photosynthesis rate, transpiration and stomatal con ductance by 68.6%, 22.7%, 97.7%, 83.9% and 84.9%, respectively, in non-AM plants, meanwhile 46.8%, 13.4%, 50.3%, 44.8% and 54.7%, respectively, in AM plants. In addition, drought increased ammonia and proline by 31.8% and 162%, respectively, in non-AM plants, while 20.9% and 76.9%, respectively, in AM plants. These results clearly showed that mycorrhizal colonization significantly relieved the responses of physiological parameters to drought stress in white clover.
Mycorrhizae Effects on N Uptake and Assimilation Estimated by
N Tracing in White Clover under Water-Stressed Conditions
Zhang, Qian ; Park, Sang-Hyun ; Kim, Tae-Hwan ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 277~284
DOI : 10.5333/KGFS.2011.31.3.277
To investigate the effects of arbuscular mycorrhizal (AM) symbiosis on N uptake and its assimilation under drought-stressed conditions in white clover, total
amount incorporated into
, amino acids and soluble proteins were quantified by
tracing during 7 days of water treatment. Under well-watered conditions, there were no significant effects of AM symbiosis on all parameters analyzed in this study. Drought stress decreased total
amount both in AM and non-AM plants, with a lower rate in AM plants (-13.8%) relative to non-AM plants (-28.5%) at day 7. Drought significantly increased
amount in non-AM plants. The amount of
-amino acids was 1.26-fold and 1.33-fold higher, respectively, in leaves and roots of AM plants compared to those of non-AM ones. Drought decreased the amount of
-soluble proteins in leaves at day 7, with a higher rate in non-AM plants than in AM ones. These results clearly indicate that AM colonization effectively alleviating the decrease in N uptake, amino acids and proteins synthesis caused by drought stress.
Effect of Seeding Rate (Sheep fescue 70% + Wildflowers 30%) on the Growth Characteristics, Seasonal Anthesis Distribution and Botanical Composition in Wildflower Pastures
Lee, Byong-Chul ; Lee, In-Duk ; Lee, Hyung-Suk ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 285~294
DOI : 10.5333/KGFS.2011.31.3.285
This study was carried out in the Chungnam National University grassland experimental field from October, 2007 to December, 2009 in order to find out the growth characteristics of the wildflowers, the seasonal anthesis distribution of the wildflowers, and the botanical composition of the wildflowers which were altogether composed of 70% sheep fescue and 30% wildflowers. The experimental species contained 34 species in total to which sheep fescue (fundamental turfgrass), 4 native wildflowers, and 29 introduced wildflowers belonged. At the time of the anthesis of the wildflowers, the average length was 29.9 cm one year later and 38.4 cm two years later. They bloomed out into nine colors, but into one or three colors during August, September, and October. The blooming season leant toward May and June and from August there was lots of difficulty in the wildflower blooming and the maintenance of their consistency. Especially, at the age of two (2009) the color, seasonal distribution, and consistency emerged as problems owing to the reduction of annual wildflowers after winterization. The annual ration of wildflowers, sheep fescue, and weeds came to 25%, 61%, and 14% each one year later (2008) and 22%, 65%, and 13% each two years later (2009). Based on this result, cultivating wildflower grassland in the ratio of 70% (sheep fescue) to 30% (wildflowers) is the proper method not only for maintaining green space by sheep fescue, but also for commanding a fine view of wild flowers.
Effects of Harvest Stages and Ensiling Method on Nutritive Values and Quality of Sorghum × Sorghum Hybrid Silage
Choi, Ki-Choon ; Song, Chae-Eun ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 295~304
DOI : 10.5333/KGFS.2011.31.3.295
This study was carried out to investigate the effect of harvest stage of Sorghum
Sorghum Hybrid (SSH) and manufacture methods of SSH silage on nutritive values and quality of SSH silage manufactured with SSH grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA. SSH "SS405" was harvested at two different growth stages (heading and ripen stage) and ensiled at each harvest stages. The content of crude protein in both square baled SSH silage (SBSS) and bag silage (BS) increased with delayed harvest maturity, but the contents of ADF (acid detergent fiber), NDF (neutral detergent fiber) decreased. The contents of ADF and NDF was not influenced by the inoculation of lactic bacteria. The contents of TDN (total digestible nutrient) in both stage and in vitro dry matter digestibility (IVDMD) in heading stage was not influenced by the harvest stage of SSH. The pH in all SSH silage ranged from 3.8 to 4.4 at two different harvest stages, and pH in heading stage was higher than that of ripen stage (P<0.05). The content of lactic acid of all SSH silage increased with delayed harvest maturity (P<0.05), but the content of acetic acid decreased (P<0.05). The contents of lactic acid and acetic acid in ripen stage were not influenced by manufacture method of silage. The content of lactic acid in both SBSS was higher than that of both BS and RBSS in heading and ripen stage (P<0.05), but the content of acetic acid decreased (P<0.05). The contents of lactic acid and acetic acid in BS were similar as compared to that of RBSS. Therefore, we suggest that the quality of SSH silage in both heading and ripen stage can be improved by manufacture methods of SBSS and BS.
Study on Manufacture of Corn Silage Using Gunny Bag
Choi, Ki-Choon ; Jo, Nam-Chul ; Jung, Min-Woong ; Lee, Kyung-Dong ; Lim, Young-Chul ; Kim, Won-Ho ; Baek, Kwang-Soo ; Kim, Maeng-Jung ; Seo, Sung ; Kim, Cheon-Man ; Lee, Soo-Sung ; Park, Kwon-Sun ;
Journal of The Korean Society of Grassland and Forage Science, volume 31, issue 3, 2011, Pages 305~314
DOI : 10.5333/KGFS.2011.31.3.305
This study was conducted to investigate the effect of harvest stage of corn on the quality of gunny bag silage manufactured with corn grown in paddy land of Department of Animal Resources Development, National Institute of Animal Science, RDA from 2009 to 2010. Corn "Kwangpyungok" was harvested at three different growth stages (milk, yellow ripen and ripen stage) and ensiled at each harvest stages. The content of crude protein (CP) of corn in gunny bag silage decreased with delayed harvest maturity, but the contents of ADF (acid detergent fiber), NDF (neutral detergent fiber), TDN (total digestible nutrient) and in vitro dry matter digestibility (IVDMD) were not changed with delayed harvest maturity. The contents of moisture, pH, and the nutritive values at three different harvest stages were not influenced by the method of silage manufacture and inoculant. The content of lactate in corn bag silage at milk stage was significantly increased (P<0.05), as compared with that of round baled corn silage. However, The contents of lactate in corn bag silage at yellow ripen stage and ripen stage were significantly decreased (P<0.05). Flieg`s score in corn bag silage manufactured at milk stage increased as compared with that of round baled corn silage, and Flieg`s score was hardly influenced by inoculant. Therefore, we suggest that manufacture method of bag silage can be new silage technique to improve the fermentation of corn silage and that smallscale stock farmer could be substituted bag silage for roll bale silage on small farm land.