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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 15, Issue 2 - Nov 1992
Volume 15, Issue 1 - May 1992
Selecting the target year
Studies on the Optimal Time for Therapy of Fasciola spp. Infected Cattle in Central Area of Korea
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 1~6
A survey on the dosage optimal time for the bovine fascioliasis was carried out from Jan. 1987 to Dec. 1990. The results were summarized as follows ; 1. The mean infection rate was 27.7% (1,662 heads were infected out of 3,247 heads In 205 herds). A seasonal infection rate was winter (34.6%) >spring(32.5%) >summer(24.7%) > autumn(22.8%) 2. In the density of the fasciola spp. egg of infected bovine feces remarks 79.6% on the range below 10. 3. The density of fasciola spp. egg is not directly proportinal to the infection rate, and remarks on the range from 5 to 20. 4. In the herds dosed vermifuge the appearance of the fasciola spp. egg is as follows ; 1∼3 months show 3.8% and 4∼6 months 8.5% 5. The dosage optimal time for the bovine fasciliasis is as follows ; 1) According to the monthly infection rate the first dosage optimal time is Dec and Feb and the second-Jun and Aug. 2) In the herd dosed vermifuge the dosage optimal time is to dose twice by 60 days interval after 3∼4 months from previous dosage.
Prevalence of Intestinal Parasites Infection of Dogs in Chonbuk Province
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 7~16
In order to detection of the Intestinal parasites, 503 fecal samples were taken from mongorel-and pad-dogs in Chonbuk province. The prevalence and identification of intestinal parasites were determined by the fecal examinations using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows 1. Fifty-nine percent (297 dogs) from 503 fecal samples were detected eggs. In seasonal detection rate of eggs, Summer was 30.3%, Autumn 26.4%, Winter 22.3% and Spring 21.0%, in order, 2. A total of 20 kinds of eggs were isolated from feces, and it was identified 75.7% as Nematoda(320 dogs), 5.6% as Cestoda(24 dogs) and 1.4% as Trematoda(6 dogs), and 17.2% as Protozoa(73 dogs). The isolates were identified as Ancylostoma caninum (30.4%, 153 dogs), Isospora spp. (14.3%, 72 dogs), Toxocara canis(11.1%, 56 dogs), Toxascaris leonina(5.8%, 29 dogs) , Uncineria stenocephala or Physaloptera spp. (5.4%, 27 dogs), Trichuris vulpis(2.4%, 12 dogs) and the others, single or in combination. 3. In mixed infection such as single, double, triple and quadraple was 63.6%, 31.7%, 3.4% and 1.3%, respectively.
Efficacy of Anthelmintic According to Guide for Fasciola spp. Infected Cattle( III )
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 17~25
For evaluation on effects guiding fasciola prevention of which the specimens collected from 6,011 cows rears at 387 farms in inchon were tested to egg count examination for 4 years(1987-1990), and the questionnaire checked up 181 dairy farms for 2 years (1989-1990). The results were summarized as follows. 1. A yearly variation of fasciola infection rate above 31% were 83% In 1987, 58% in 1988, 25% in 1989, noninfection in 1990. 2. The average concentration in feces of infected dairy cattle was 79.4% below 1-10 eggs for 4 years(1987-1990) 3. In the results of the questionnaire for 2 years(1989-1990) the farmers gradually took interest in fasciola prevention. 4. To re-dose anthelmintics was 75.4% in 1989 and 82.5% in 1990 after 5∼7 months from previous dosage.
Studies on Protective Efficacy of Escherichia coli Vaccines
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 26~31
The oil emulsion and alhydrogel vaccines were prepared from a strain of enterotoxigenic Escherichia coli isolated from calves with diarrhea and their protective efficacy and immunogenicity were tested in Guinea-pigs. Enterotoxigenic Escherichia coli, isolated from calves with diarrhea, has K99 and F4l antigen as 46.2％ and 50.9％ with 48 and 53 strains respectively out of 104 strains. The protective efficacy of the gel and oil vaccines were 60% and 80% respectively. Agglutinin titers to sera of Guinea-pigs vaccinated with experimental gel and oil vaccines peaked at 5 and 6 weeks after vaccination.
Detection of Antibody to Infectious Laryngotracheitis Virus by Enzyme Linked Immunosorbent Assay
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 32~45
In order to establish and enzyme-linked immunosorbent assay to ILTV, field virus strain of ILTV was propagated in chorioallantoic membrane of the embryonated eggs. purified and used as antigen. The antisera selected from the field samples and immunized chickens based on serum neutralization test were used as the standard positive and negative sera in all tests. It was found that optimal antigen concentration was
of protein per well and a 1 : 100 dilution of standard serum showed low background optical density with negative serum and high P/N values of positive sera. A 1 : 500 dilution of the rabbit anti-chicken IgG peroxidase conjugate produced a high P/N values and thirty minutes was chosen as suitable time to read the optical density of the enzyme substrate reaction and optical density was consistent during the 16 hours after stopper was treated. When coated antigen was kept on microplate for varying time up to 16 hours at
no significant difference was observed between the treatment. The coated antigen could be kept without change of antigenicity for at least one month at
and room temperature. When blocking buffer contanining bovine serum albumin was mixed directly with conjugate and serum at 10% level induced higher P/N values compared to blocking antigen coated microplate with the same blocking buffer. The coefficience of correlation between ELISA and SN test was 0.577. When antibody response of chickens, vaccinated with ILTV, was examined by ELISA and SN test, antibody rising and decay pattern between the two test was similar until 11 weeks of age. However 12 weeks onward antibody titer checked on by SN test was slightly lower than that tested by ELISA.
Prevalence of Leptospiral Antibodies in Pigs
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 46~50
This experiment was to investigate the Leptospiral antibody in the pigs with the serological test by the microscopic-agglutination-test (MAT) from November 1991. to Januaury 1992. Antigen(living antigen) was used L. icterohemorrhagiae, L. pomona, L. canicola, L. hardjo, L. ballum, L. australis, L. autumnalis, L. grippotyphosa, L. tarassovi, L. pyrogenes, L. bataviae, L. hebdomadis for the serolgical test in the pigs. The result obtained are summarized as follows of the total 202 serum samples examined, 1. Among the serum samples of 202 heads, 19 heads of the pigs(9.4%) were positive. 2. Among the positive samples of 19 heads, The detected were L. icterohemorhagiae 10 heads(5.0%), L. pomona 3 heads(1.5%), L. canicola 6 heads(3.0%). 3. Antibody titers of positive sera were ranging from 1：100 to 1 : 400. Serotiters appeared to be very low, 4. The seroprevalence of Leptospira in Chechon was higher than that other districts (5. 4% -5.8%), but the lower than Chung-nam, Kyonggi(13.7-15.9%)
Survey on Antibodies Against Bovine Leukemia Virus in Chung Buk Province
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 51~57
A serological survey was carried out for the detection of antibody of Bovine Leukemia Virus (BLV) in nothern parts of Chung Buk area. The results were summarized as followed. 1. The overall positive rate was revealed as high as 15% with 48 positive cases out of 319 heads examined. 2. According to age, cattle of 4 to 7 ages showed relatively higher positive rate of 15% than other ages. 3. Seasonal differences of positive rate were not recognized. 4. BLV antibody titer of scales of cattles that from 5 to 15 heads farm were the highest. 5. With the result of blood test that of BLV positive cattle, the number of WBC was slightly Increased, but other records were normal.
Changes in Glucose Concentrations and Activities of Cholinesterase in Serum, Brain and Spinal cord in Mice following Orally Administration of Parathion
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 58~66
The insecticide p-nitropheny diethyl thiophospate is alse known by the symbol E.605 and a legion of trade names including “parathion”. The insecticide is widely used in agriculture, but it is highly toxic and now clear that parathion behaves like a cholinergic drug by inhibiting the enzyme cholinesterase. In order to know acute toxicity and the changes of glucose concentrations and activity according to time lapsed in female mice given orally single with the half dose to
of parathion, glucose contents and cholinesterase activities in serum as well as cholinesterase activities in whole brain and spinal cord were investigated, otherwise median lethal dose (
) of parathion given orally against female mice was determined. The results obtained were summerized as follows ; 1.
value of parathion given orally to female mice was 7.1mg/kg(95% confidence limits, 3.8-13.1mg/kg) 2. The inhibition rate of cholinesterase activities in serum of parathion-administrated mice according to time lapsed were peakly decreased to 61% after 30 minutes in comparison to control group, but activities were completely recovered after 48 hours. 3. The inhibition rate of cholinesterase activities in whole brain of parathion-administrated mice according to time lapsed were peakly decreased to 49% after 2 hours in completely recovered after 24 hours. 4. The inhibition rate of cholinesterase activities in spinal cord of parathion-administrated mice according to time lapsed were peakly decreased to 57% after 2 hours in comparison to control group, but activities were completely recovered after 48 hours. 5. The changes of glucose contents in serum of parathion-administrated mice according to time lapsed and in directly after death due to parathion poisoning were no significantly difference.
Studies on Properties of Avian Reovirus Isolated in Korea
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 67~80
Avian reoviruses have been implicated in respiratory disease enteric conditions including Cloacal pasting in young thicks, pericarditis, hydropericardium, anaemia with swollen spleen and liver and petechiation of skeletal muscle and viral arthritis. This study was conducted to examine properties of reovirus field 3 strains isolated from affected broiler from several farms. An infectious agent was isolated from leg tendons and intestine of broiler with clinical tenosynovitis. The agent grew well on the chicken embryo kideny cells(CEK). One of them produced cytopathic effects(CPE) of round type and formed intranuclear inclusions, and the other was characterized by CPE of syncytical type and cytoplasmic inclusion. The properties and serological classification of field strains were examined by hemagglutin test, virus neutralization test, agar gel precipitin, electropherotype. They showed no hemagglutination reactions and not well neutralization and to possess common antigens detectable by AGP test. RNA electropherotype presented 10 segment band as the previous report. These data suggest that the field strains and standard strains (1133, 1733) may be the same group.
Isolation, Identification and Drug Susceptibility of Bacteria from Genital Organs of Slaughter Sows
Korean Journal of Veterinary Service, volume 15, issue 1, 1992, Pages 81~88
This study was conducted to determine the microorganisms inhabitating in sow genital organs and their anti-microbial drug susceptibility During the period between February, 1991 and November 1991, 128 sow genital organs were sampled at six abattoirs. Gross pathological examination and bacterial isolation and identification were performed from the genital organ. In addition, antimicrobial drug susceptibility for the major organisms isolated were examined. 1. Among the bateria isolated from normal genital organs, E. coli(30.7%) Stahylococcus spp.(29.4%), Corynebarterium pyogenes(C. pyogenes) (14.7%), Streptococcus spp.(13.3%) were most freqently isolated, whereas the genera of Klebsiella, Actinobacillus, and Serratia were detected less freqently. 2. Among the bacteria isolated from abnormal genital organs, C. pyogenes,(37.7%), Stahylococcus spp.(30.2%), Proteus spp. (26.4%) , Pasteurella spp. (18.9%) , Steptococcus spp. (9.4%) were most freqently isolated whereas the genera of Pseudomonas, Serratia and Klebsiella were detected less freqently. 3. From sow genital organs showing lesion of endometritis and purulent endometritis C. pyogenes were most freqently isolated, the isolation rate being 67.7％ and followed by Stahylococcus spp., E. coli, Proteus spp., Steptococcus spp. and Pasteurella spp. in the order. 4. Antimicrobial drug susceptibility of the major organisms showed that all the isolates were susceptible to cephalothin, ampicillin, chloramphenicol and sulfamethoxazole / trimethoprim, but resistant to penicillin and streptomycin.