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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 15, Issue 2 - Nov 1992
Volume 15, Issue 1 - May 1992
Selecting the target year
Detection of Antibiotic Residues in Meats and Internal Organs of Cattle and Pigs
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 93~100
The purpose of the present survey was to evaluate the antibiotic residues in meats and internal organs such as muscle, liver, heart, kidney and spleen of cattle (n=59) and pigs (n=115). The EEC-4-plate-method were employed. The results were obtained as follows ; 1. In BS 6.0, BS 7.2 and BS 8.0 used as media to detect antibiotic residues, the zone(
cm) of bacterial growth inhibition was narrow(
) in meats, whereas the zone was wide(
in cattle and pigs, respectively) in internal organs. But in SL 8.0, it was difficult to detect the zones (
) of both meats and internal organs. 2. Residues of antibiotic in beef and pork were rarely detected in BS 6.0, BS 7.2 and BS 8.0 (range 1.7-11.9% and 2.6-4.3%, respectively), whereas residual percentages of internal organs were relatively higher(range 69.5-96.6% and 43.5-84.3%, respectively). But in SL 8.0, it was not detected in both beef or pork, whereas they were 0-13.6% and 0-4.3% in interanal organs.
A Study on Effect of Sulfamethazine and Trimethoprim on T.T.C.(2,3,5-Triphenyl Tetrazolium Chloride) Test in Raw milk
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 101~108
This study was carried out to investigate the effect of sulfamethazine and trimethoprim on TTC test in raw milk. Ten raw milks which does not contain any antibiotics were collected from milk farms and one hundred twenty raw milks which collected from three raw milk receiving stations and those milk samples were mixed up sulfamethazine and trimethoprim for TTC test. The results were as follows ; 1. Positive reactions of TTC test were shown above the concentration of 3, 000ppm for sulfamethazine and 10ppm for trimethoprim. 2. When the TTC test was conducted with trimethoprim and sulfamethazine mixture, a pair of the concentration of trimethoprim and sulfamethazine shown to positive reaction were 0.1-2, 000ppm, 0.5-1, 000ppm, 1.0-250ppm and 2.0-100ppm, respectively. 3. Of 120 raw milk samples tested, 16 samples(13%) were shown quasi-positive reaction when the trimethoprim at the concetration of 2ppm was added in the samples.
A Survey of Sarcocystis Infections in the Slaughtered Cattle and Identification of Sarcocystis cruzi
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 109~120
330 Samples of the slaughtered cattle heart muscle were collected from the abattoirs of five regions in Kangwon - do to reveal the frequency of sarcocystis infections during January through December in 1991. The samples were inspected for bradyzoites by the trypsin digestion technique and the possitive samples were fed to dogs and cats for the detection of sporocysts shed in the feces. The results obtained were summarized as follows : 1. The infection rate of bovine Sarcocystis investigated from 330 samples was 43.6%. 2. It revealed that the infection rate of Sarrocystis increased gradully with the advance in the age, 14.5% in below two years, 26.1% in the three years, 30% in four years, 54.7% in five years, 74.4% in six years, 90% in seven years and 100% in older than eight years. 3. The cyst walls detected out from the heart muscles were less than l
in thickness and the size of bradyzoites were
in average. 4. The size of sporocysts shed in the feces of dogs were
in average and the prepatent periods ranged from 12 to 16days. 5. Sarcorystis found in the bovine heart muscles were identified as Sascocystis cruzi ( Hasselman, 1923) , wenyon, 1926.
A Survey of Swine Internal Parasites at the Cement-floored and Sawdust Fermentative Pigsty
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 121~127
To investigate the prevalence of swine internal parasitic infection, the fecal examinations were carried out between July. 1991. and June. 1992. The samples of 3, 126 swine feces and sawdust were collected from 6 sawdust fermentative and 6 cement-floored pigsties in Chonnam area. The results obtained were as follows. 1. The rate of swine internal parasitic infection, 38.5％ from sawdust fermentative pigsty was higher than 22.2％ from cement-floored pigsty. 2. Of the swine internal parasites detected, Balantidium cole, 21.4% from growing pig of sawdust fermentative pigsty, and 21.3％ from sow of cement-floored pigsty was the must commonly encountered. The infection rate of another parasites including Trichuris suis in sawdust fermentative pigsty was also higher than cement-floored pigsty. 3. Larvae of Strongyloides ransomi was the highest infection rate (40.0%) at sawdust of the sawdust fermentative pigsty.
Effects of Time Elapse of Serum Separation on the Examination of Bovine Blood Chemical Values
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 128~133
To obtain the basic data for blood chemistry, the effects of the time elapse of serum separation on bovine blood chemical values were investigated. The results obtained are summerized as follows : 1. The constant fluctuation tendency and significance was not detected in GOT, GPT, BUN, Creatinine, ALP, CPX, Cholesterol, Ca, Mg and Pi. 2. Glucose showed the decrease tendency according to the time elapse of serum separation. As the values at 12 and 24 hours showed significant lower values than those at 1 and 2 hours (p＜0.01) it was thougth that separation time of serum should be focussed for the glucose determination.
Antibiotic Resistance of Pathogenic Escherichia coli Isolated from Piglets with Diarrhea
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 134~143
The present study was conducted to investigate the biochemical characteristics and anti-biotic resistance of Escherichia coli(E. coli) isolated from piglets with diarrhea in Kyongbuk province during the Period from February to November 1991. 368 E. coli strains were isolated from 382 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. Tetracycline and sulfadimethoxine were found to be highly ineffective at in vitro inhibition of the E. coli of piglets origin. The majority of E. coli were susceptible to amikacin, chloramphenicol and gentamicine. 89 (89.0%) of 100 strains of E. coil were resistant to one or more drugs. The organisms resistant to 20 or 3 drugs were 54(60.6%) of 89 strains, whereas 16(17.9%) strains were found to be resistant to one drug. 55(61.8%) out of 89 drug resistance strains carried R factors(
) which were transfer-able to the recipients by conjugation.
Comparison Osmotic Fragility of Erythrocyte Domestic Chicken, Duck, Quail, Dog, and Pig
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 144~149
The study was attempted to scrutinize the normal osmotic fragility of erythrocytes in domestic poultry such as chicken, quail and duck making a comparison with that in domestic mammalia such as dog and pig. Osmotic fragility of erythrocytes was determined on blood samples from 10 healthy adult animal in each species. Optical initial hemolysis of erytyrocytes occurred at
Nacl for chicken,
for dog and
for pig. Optical complete hemolysis of erytyrocytes occurred at
Nacl for chicken,
for dog and
for p. In other words, erythrocytes of poultry have stronger resistance to osmotic Iysis than that of mammalia, showing the strongest resistance In chicken among the tested poultry.
A Study on the Effects of Bovine Mastitis Control Using SCC, CMT and Pathogens
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 150~165
The study for a effect of monitoring on bovine mastitis was conduced for a year from Dec, 1989 to Nov, 1990, Sampling the bulk milk of 350 herds in Inchon city and out of 10 herds among them were carried out herds guidance, CMT, SCC, isolation of pathogens and antibiotic sensitivity tests. The results obtained were summarized as follows 1. Annual mean SCC of 1213 herds was 558, 000 cell /ml 2. The number of SCC below 500, 000 cell /ml to quarters for herds guidance was at 1st 77. 0%, End 84.8% and 3rd 80.4%. The is shown that milk quality was steadly improved. 3. The most number of isolated pathogens of bovine mastitis was Staphylococcus SPP - 402(47.2%) Streptococcus SPP - 80(18.7% ) 4. The highest rate of antibiotic sensitivity test was Stapylococcus SPP - cephalothin(76.7%) Streptococcus SPP - ampicillin(77.5%) Gram negative bacilli - tetracyclin(76.0%) 5. The effect of monitoring on bovine mastitis was improved showing that at 1st 49.0% to 3rd 72.0%
Effect of Deoxynivalenol on Immunoglobulin in the Mouse
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 166~173
Mice were fed semi-purified diets containing 0, 2, 10 and 25 ppm(mg/kg) deoxynivalenol over 8 weeks and were assessed for effects on bodyweight gain, serum immunolglobulin levels and surface immunoglobulin bearing lymphocyte ratio. 1. The rate of body-weight gain was significantly reduced (p＜0.05) at the 10 and 25 ppm of DON, whereas the mice ingesting the diet containing 2 ppm DON was not. 2. IgA in serum immunolglobulin was significantly increased (P＜0.05) at the 10 and 25 ppm of DON, but IgG, IgM were decreased, whereas exposure to 2 ppm DON was not change. 3. Concentration of IgA from Peyer's patch of mice fed DON exhibited increased at 10, 25 ppm. 4. Lymphocytes surface marker studies revealed that IgA, IgG and IgM were 2.2%, 0.4% and 1.5％ respectively. These results suggest that dietary exposure to DON alters regulation of IgA production
Survey on the Distrributions of Swine Toxoplasma Antibodies by Latex Agglutination Test in Gyeongnam Central Area
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 174~183
This study was conducted to determine the serum antibodies against toxoplasma in swine from breeding-pig farm, pig farm and abattoir by latex agglutination(LA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical co.). The results obtained were summerized as follows : 1. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum dilution of 1 ; 32. 2. positive rates of toxoplasma antibodies in 823 swine sera were 17.0%(140 cases) by LA test. 3. The toxoplasma antibody detection rates against 194 swine sera in breeding-pig farm, 273 swine sera in pig farm and 356 swine in abattoir were 46.9%(91 cases), 8.4%(23 cases) and 7.3% (26 cases) , respectively. 4. In LA test serum antibody titers in 823 test sera were shown as 51 cases (36.4%) in 1 : 32, 40(28.6%) in 1；64, 17(12.1%) in 1:128, 14(10.0%) in 1：256, 10(7.1%) in 1:512, 5(3.6%) in 1：1,024, and 3(2.1%) in 1 : 2,048. 5. Positive rates of toxoplasma antibodies in swine sera from each breeding-pig farm were 20.0∼61.9%.
Demonstration of Newcastle Disease Virus Antigens in Paraffin Embedded Tissues of Experimentally Infected Chickens Using Peroxidase-antiperoxidase(PAP) Technique
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 184~194
This study was done to identify Newcastle disease virus(NDV) antigens in paraffin sections of various organs from experimentally NDV-infected chicken using peroxidase-antiperoxidase(PAP) technique. Sections were Incubated with rabbit anti-NDV polyclonal as first antibody, followed by incubation with goat anti-rabbit IgG conjugate and peroxidase anti-peroxidase ( PAP ). Positive reactions were often detected in the epithelim of trachea and in the lymphocyte of spleen at 24 hours after virus inoculation. The viral antigen was localized mainly in the cytoplasm of infected cells. The method approved to be highly specific for the indetification of NDV and allowed a precise localization of the viral antigens in infected cells.
Pathological Study on Balantidiosis of Pigs
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 195~202
Fecal Examination for survey of natural prevalence of Balantidium coli was performed on 1,080 healthy bred pigs in Korea, and the positive rate was 56.2%. In order to observe if Balantidium coli might be a secondary invader after certain initiation of the Intestinal lesions, the piglet groups preinfected with the protozoa experimentally were treated with salmonella cholerasuis, Trichuris, cold stress, HCI, and immunosuppressive drug respectively, but no relatios was found between them. Also the protozoa were not deteceted from the intestinal lesions spontaneously formed of 107 pigs which were checked in the slaughter house. According to the above results, Balantidium coli is not directly associated with the formation of any lesions in the pig's intestine.
Investigation for Pollution of Livestock Waste in Daechong Reservoir Area
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 203~214
Livestock waste causes ground and surface water pollution, eutrophication of reservoir as well as adverse affects living environment of those who dwell nearby. In order to investigate the pollution load from livestock waste, physical and chemical characteristics of the waste were determinded in the survey of livestock farms. Once pollution load is obtained as a function of the origin units of livestock, the influence of livestock waste on the Daecheong reservoir was studied. 1. For Daecheong reservoir inflow area, the liverstock manure productions of beef cattle swine, dairy cattle and chicken were 1,135.6t/day, 480t/day, 241.3t/day, 48t/day respectively； Beef cattle was the mai or source of the pollution. Pollution loading productions due to the livestock waste around the were found to be 53.31t/day for BOD, 222.49t /day for COD, 261.99t/day for T-S, 9.64t/day for T-N, 6.54t/day for T-P 2. Bocheong stream turnd out to be the major contribution for pollution loading production to Daecheong reservoir with 10,748kg /day of BOD, 47,157kg /day of COD, 1,946kg /day of T-N, 1,271kg /day of T-p. 3. Actual pollution loadings from livestock wastes for the area of Daecheong reservoir were estimated as 1,997kg /day(BOD), 8,546kg /day(COD), 364kg /day(T-N), 243kg /day (T-P), respectively Therefore, advanced treatment for livestock waste is recommended for Daectleong reservoir inflow area to eliminate the nutrients which are major sources of eutrophication of the reservoir.
Observations on Livestock Wastewater in Taegu Area
Korean Journal of Veterinary Service, volume 15, issue 2, 1992, Pages 215~225
To estimate pollution status of livestock wastewater on four piggeries and one abattoir in Taegu area, physicochemical water analysis such as pH, suspended solid(SS), biochemical oxygen demand(BOD) and chemical oxygen demand(COD), and bacteriological examinations such as number of total viable cells and number of coliform with or without antibiotic resistance were carried out. The results obtained were as follows : The pH values of raw sewage ranged from 9.0 to 7.2 that of the effluent treated was lowed to 5.6~7.7. The SS values of raw sewage ranged from 5,275ppm to 120ppm and those of the efflunet decreased to 162~30ppm. The BOD values of raw sewage ranged 6,200ppm to 120ppm and those of the effluent treated decreased to 111 ~80ppm. The COD values of raw sewage ranged from 5,725ppm to 298ppm and those of the effluent decreased to 137~76ppm. The total viable cells of raw sewage ranged from
/ml, those of the effluent decreased to
The total coliforms of raw sewage ranged from
/ml, those of the effluent decreased to
/ml. The incidence of streptomycin resistant coliforms was the highest(1.8~66.7%), and followed by tetracycline(1.7~64%), kanamycin(9.3~50.l%), ampicillin(0.06~45.5%) and chloramphenicol(14.3~33.5%) to total coliforms of raw sewage. The incidence of antibiotic resistant coliforms of raw sewage in farms ranged from 3.4~66.7% and that of abattoir's was 0.06% to 14.3%. Antibiotic resistant coliform counts of raw sewage ranged from 1.3
/ml to 3.9
/ml, those of the effluent decreased to