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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 16, Issue 2 - Nov 1993
Volume 16, Issue 1 - May 1993
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Comparison of Fossomatic and Coulter Counter Methods for Somatic Cell Count in Raw Milk
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 1~10
Samples of bulk herd milk, foremilk, last milk (stripping) and individual cow sample were collected and their somatic cell number were counted with Fossomatic counter (FCC), Coulter counter(CC), direct microscopic somatic cell count(DMSCC) and Califormia mastitis test (CMT), The results were compared and summarized as follows : 1. Mean somatic cell counts of 120 bulk herd milk samples obtained by DMSCC, FCC and CC were 433,203, 481,213 and 676,245 respectively. 2. Mean somatic cell counts of 116 foremilk samples obtained by DMSCC, FCC and CC were 515,035, 611,845 and 725,051 respectively 3. Mean somatic cell counts of 87 last milk samples obtained by DMSCC, FCC and CC were 718,506, 839,874 and 1,041,160 respectively. 4. Mean somatic cell counts of 57 individual cow samples obtained by DMSCC, FCC and CC were 449,258, 491,018 and 521,315 respectively. 5. Mean somatic cell counts of all samples increased with the increasing CMT score, and the cell counts were higher by CC than by FCC. 6. The correlation coefficients between the somatic cell counts by CMT and CC were 0.926 in bulk herd milk, 0.707 in foremilk 0.688 in last milk and 0.675 in individual cow sample, respectively 7. The correlation coefficients between the somatic cell counts by CMT and FCC were 0. 945 in bulk herd milk, 0.705 in foremilk 0.694 in last milk and 0.727 in individual cow sample, respectively. 8. The correlation coefficients between the somatic cell counts by CC and FCC were 0.978 in bulk herd milk, 0.997 in foremilk 0.983 in last milk and 0.985 in individual cow sample, respectively.
A Study on Detection Method of Sulfonamides Residues in Raw Milk by HPLC
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 11~19
This experiment was carried out to detect the residues of sulfonamides in raw milk. Raw milks which does not contain sulfonamides was collected from one of the farm and fortified with 5 sulfonamides (sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine, sulfaqinoxaline). The sulfonamides in the fortified sample were extracted and detected by High Performance Liquid Chromatography. UV /vis detector was used in this experiment. The results obtained were summarized as follows : 1. Chloroform was good as a extracting solution. 2. 15.5% methanol in PDP as a mobile phase solution was best detective condition for SMR, SMT, SMM. But for SDM and SQN the best condition was 23% methanol. 3. The detectable limits of SMR, SMT, SMM were 2ppb. but SDM and SQN were 20ppb because of delayed retention time and relatively low recovery rate. 4. The peaks of SMR, SMT, SMM and SDM were erected at baseline and the apexes were sharp but SQN was round shape.
Effects of Preservatives on Inhibition and Survival of Listeria Monocytogenes
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 20~33
The studies were conducted to determine the effects of preservatives such as sodium chloride, sodium nitrite, sodium benzoate and sorbic acid on the survival of L. monocytogenes with regard to interaction of temperature, heat and pH of the medium. Inactivation of L. monocytogenes Scott A was more predominent by combination of sodium chloride and the other preservatives than sodium chloride alone, and inactivation was more exhilarated at
The organism was not inactivated when sodium chloride, sodium nitrite, sodium benzoate and sorbic acid were added to 3%, 100ppm, 0.1, or lower, respectively, but was inactivated in the concentration increased twice. In TSB(tryptic soy broth) at pH 5.0 or lower, the organism did not grow regadless of the kinds of preservatives, and inactivation effect particularly was prominent in the presence of sodium nitrite and sorbic acid. On the other hand, at pH 6.0 or higher L. monocytogenes gradually increased in numbers and the effects of inhibition was higher in the presence of sorbic acid than in the other preservatives. When the preservatives were added to the concentration commonly used, incubation in TSB at
gradually resulted in growth of the bacterium and the organism rapidly decreased in numbers at
after incubation for 1 week. When L. monocytogenes was inoculated in TSB containing various preservatives and heated at
for 30minutes, the organism decreased in numbers at all preservatives. Particularly, viability rate of the organism was the lowest as 0.07% in the presence of sorbic acid.
Survey on Atrophic Rhinitis Caused by Bordetella Bronchiseptica from Slaughtered Pig in Kyeonggi Province
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 34~40
The present study was conducted to investigate the isolation frequency, biochemical prop erties and antimicrobial susceptibility of B. bronchiseptica isolated from slaughtered pigs during the period from March to December, 1992. In Kyeonggi province. A serological survey for antibody of B. bronchiseptica in 200 slaughtered pigs was carried out by agglutination and tetrazolium reduction methods. The results were summarized as follows ; 1. From 80 slaughtered pigs, 27(33.8%) case were isolated and all isolate strains were resistant to Penicillin, Streptomycin, Chloramphenicol, Tetracycline and Ampicllin, while the majority of them were susceptible to Gentamicin, Cloxacin, Colistin, Neomycin, and Kanamycin. 2. Incidence of B. bronchiseptica antibody in 200 slaughtered pigs were measured by agglutination and tetrazolium reduction methods. Agglutination method was shown 38 (19%) of 200 with a titer of below 1：20 and 20(10%) of 200 with a titer of above 1：640. Tetrazolium reduction method was observed 33(16.5%) of 200 with a titer of below 1 : 20 and 32(15%) of 200 with a titer of above 1：640. 3. LSD analysis indicated that the difference of the responses between agglutination test and tetrazolium reduction test was not significant.
A Study on Bioserotype and Drug Resistance of Salmonella and Escherichia coli Isolated from Feces in Zoological Animals
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 41~50
This study was undertaken the bioserotype and drug resistance of Salmonella and Escherichia coli isolated from feces for the prevention and treatment of salmonellosis and colibacillosis in zoological animals. The results obtained from the research were as follows 1. Salmonella were isolated 19, or 4.7% from 408 samples and E. coli were isolated 12, or 40.0% from 30 diarrheal samples. 2. The biotypes in 19 Salmonella were Subspecies 1. 3. The serogroups of Salmonella isolated were 47.4% in B group, 31.6% in C, 5.3% in D and 15.8% in other, and serotype of E. coli was 100% in 0127a. 4. The antibiotic resistance of Salmonella and E. coli isolated were 13, or 68.4% and 7, or 58.3% strains, respectively 5. The multiple resistant patterns of antibiotics in Salmonella were 2drugs- and 3 drugs-resistance 30.8%, respectively, and those in E. coli were mono drug-, 2 drugs- and 7 drugs-resistance 28.6%, respectively. 6. The transferred rate of resistance to recipients (E. coli ML 1410 NA
) in Salmonella was 38.5%, but that in E. coli was 71.4%.
A Study on the Characteristics and Incidences of T. hyodysenteriae on Pigs.
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 51~56
Feces were collected from pigs of various ages on slaughter house and swinery in the southern area of the kangwon-do. Treponema hyodysenteriae were isolated from the feces by the method of Jenkinson. and the effects of temperature on the survival of these organism were investigated. The results abtained are summerized as follows ; 1. T. hyodysenteriae were isolated from 22 fecal samples of 1, 296 samples, and showed higher incidence at 4-8week age groups than other age groups. 2. The organisms were isolated from 17 samples of 329 diarrhea samples and 5 samples of 967 non-diarrhea samples. 3. T hyodysenteriae survived long at
but survived short at
Liver Cirrhosis of Korean Native Goat in Korea : A Case Report
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 57~64
This survey was performed to report rare outbreak of liver cirrhosis in Korean native goat (KNG) which was died of Yangpyeong's goat farm on Feb. 1992. The examination for the KNG was carried out by clinical signs, necropsy and various lab-oratory test including parasitic, bacterial and histological test. The KNG looked jaundice, ascite, hemorrhage of lumen, abomasum and intestine, and brownish smooth cirrhotic liver at necropsy. Histological examination for liver revealed considerable proliferation of connective tissue and piecemeal necrosis which was caused by chronic active inflammation in interlobules and intralobules. There were atrophic micro and macro nodules which were sur-rounded by connective tissue. The lobular structure lack almost all central vein. The portal areas appearred proliferation of bile ducts, blood vessels and connective tissues. These connective tissue infiltrated heavily with plasma cells, Iymphocytes and histocytes. Histological examination for brain proved to be hepatic encephalopathy by virture of congestion and edema in cerebral medullary. From these results were demonstrated miked nodular, active, postnecrotic liver cirrhosis.
Prevalence of Leptospiral Antibodies in Sows
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 65~69
A serological survey was conducted to detect the type of Leptospirosis in sows. Antigen(live antigen) tested were L.icterohemorrhagiae, L.pomonal, L.canicola, L.Hardjo, L.australis, L.autumnalis, L.grippotyphosa, L.pyrogens, L.bataviae, L.hebdomadis. The survey was performed from J one 1992 to December 1992 by microscopic-agglutination test. The results were as follows 1, Among the serum samples of 92 heads of the sows, 6 heads of the sow(6.5%) were positive. 2. Among the positive samples of 6 heads, L.icterihemorrhagiae were 4 heads(4.3%) and L. pomona 2 heads (2.2%), respectively. 3. Antibody titers of positive sera were ranging from 1：200 to 1：1600. 4. The seroprevalence of leptospira in Chechon city, Chechon county, Danyang county that Chechon county(3.3%) was higher than that of other districts(1% -2.2%).
Studies on the Prevalence of Bovine Rotavirus Infection
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 70~75
To determine the prevalence of bovine rotavirus Infection in Chungbuk area, fecal specimen were collected from calves nth diarrhea and tested using ELISA. The positive rates were 53.8%(1 to 30days old), 19.0%(31 to 60days old), and 3.2%(over 60days old). Electrophoretic migration patterns of genomic RNA from field isolates were similar to that of NCDV strain, prototype of bovine rotavirus. Bovine rotavirus field isolate showed characteristic morphology of rotavirus particle with 80nm in diameter, using EM.
Studies on the Efficacy of Infectious Laryngotracheitis Vaccine
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 76~81
To evaluate the pathogenesis of live infectious laryngotracheitis vaccine, virus, virus and E.coli were inoculated in 4-weeks old intraorbitally and intraorally. Their pathology and serology were studied. The results were as follows. 1. thicks Inoculated with ILT vaccine alone showed no clinical sign. 2. Some of chicks inoculated with ILT vaccine followed by E.coli after 1day showed nasal discharge and cough. 3. Some of chicks inoculated with E. coli followed by ILT vaccine after 1 day and inoculated ILT vaccine and E.coli concurrently, showed nasal discharge. 4. Serum neutralization titers of chicks inoculated with ILT vaccine increased 3 weeks after Inoculation, peaked at 7 to 8 weeks and decreased. 5. Antibiotic sensitivity of E.coli were higher in Enrofloxacin (Baytril) and chloramphenical.
Prevalence of Parasite Infection of Fowls in Chonbuk Province
Korean Journal of Veterinary Service, volume 16, issue 1, 1993, Pages 82~89
In order to detection of the intestinal parasites, fecal samples were taken from broiler (n=262), laying hen(n=244), parent stock(n=207) and native stock(n=287) in Chonbuk province. The prevalence and identification of intestinal parasites were determined by the fecal examination using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows : 1. The detection rate of parasite-eggs from 4 flocks(total=1,000) was 65.7%. 2. In the breed and type of breeding, infection rate of parasite-eggs was detected 84.0％ as native stock (floor breeding, 241 chicken), 79.7％ as parent stock (floor breeding, 165 chickens), 73.3% as broiler(floor breeding, 192 chicken) and 24.2% as laying hen(cage breeding, 59 chicken), in order. 3. In the concern of mixed infection such as single, double and triple, the rate was 55.1%, 8.7% and 1.9%, respectively. 4. Ten kinds of infective eggs were isolated from 657 fecal sample of 4 flock. They were classified 51.l% as Eimeria spp., 12.7% as Ascaridia galli, 5.1% as Capillaria spp., 4.1% as Strongyloides avium, 2.3% as Heterakis gallinarum, 0.5% as Hymenolepis spp., 0.3% as Railleina spp. and 0.2% as Syngamus spp., Trichostrongilus spp., or Choanoteania spp., single or in combination.