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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 20, Issue 4 - Dec 1997
Volume 20, Issue 3 - Nov 1997
Volume 20, Issue 2 - May 1997
Volume 20, Issue 1 - Mar 1997
Selecting the target year
The latent period and anti-epidemic measure of bovine brucellosis
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 1~9
The 37,000 holstein which had been raised in Jeju island were examined for the purpose of brucellosis detection and so on. We investigated the relationship between the farms showing positive outbreak and the number of examination, incubation period, and the outbreak rate of positive cattle. We endeavored to separate germs from brucellosis positive cattles blood and negative bulls semen against brucellosis serum test and concluded as follows. We intend to offer you institutional supplements and preventions of brucellosis for the future. 1. Outbreak of brucellosis could not lead the outbreak rate of positive cattle to decrease through 1-2 examination per year, For the past 4 years, the outbreak rate of brucellosis positive cattle had a tendency to show a gradual decrease through at least 5 examinations per year. 2. As we examined live-in cattle with positive cattle in mass outbreak farms for 9 months, we found out positive cattle every month. In a grazing land, the group of cattle producd enormous positive cattle in 9-12 months. 3. Annual brucellosis positive outbreak rate was 20-25% among live-in cattle with brucellosis positive cattle, but the rate might be 50-100% depending on extent of pollution. 4. 94's brucellosis examination showed that 200 positive cattle of 71,153 cattle and most of them were live-in cattle with confirmed brucellosis positive cattle. 5. 1 head of 200 positive cattle showed positivity against serum of bulls test and the rate of bulls was about 1%. 6. Brucellosis germ was separated from 2 cattle's semen among 52 negative bulls against brucellosis serum test and there was the one brucellosis positive cattle against tube agglutination of semen test, so the positive rate of brucellosis was 5.8%. 7. Brucellosis germ was not separated from 15 brucellosis positive cattle's blood.
Studies on the Edwardsiellosis of cultured flounder, Paralichthys oliuaceus;- Characteristics, pathgenicity and control of Edwardsiella tarda -
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 11~18
Characteristics, pathogenicity and control of the causative organisms isolated front diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF-1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KBF-1) with
cfu/fish were died within 60 hrs, but 0~9 flounders infected group with
cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with
cfu/fish were died within 36 hrs, while 0~7 flounders infected with
cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-1 strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-1 strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.
The variation of serological titers on the chickens infected pullorum disease from Kyongbuk provinces
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 19~26
The present study was conducted to investigate the general epidemiological situations with 18-pullorum infected chickens from Kyongbuk provinces during the period from June 1995 to January 1996. On the Salmonella pullorum isolation tests by rectal swab culture method from infected chickens (386-samples), any Salmonella spp was not isolated from infected live-birds. But 2-S pullorum were isolated of 2-dead chickens(33.3% ) from 6-dead chickens which were positively reacted by serological tests. On the other hand, we could not isolated any Salmonella spp. in any parts of egg-contents ; egg-shell, egg-white and egg-yolks with 25-infected bird eggs. On the tests of antibiogram, 2-S pullorum strains were highly sensitive to GM, AM, SXT, CZ, K, FIM, ENR, C, AN, N, NN, LIN＋SP, CF, TE and PB, respectively and intermediate sensitive to the CB, CFP, CL, S, P and XNL. But 2-strains were resistant to CC, DP, E, L, OX, TLA and TyLO. In the serological tests, pullorum antibody titers of 18-infected birds was from 2.76 to 9.18 with average by the microplate test. During the 6-months, pullorum antibody average titers were not changed generally. To validate the effects of the antimicrobial agent treatments to the serological antibody titers, infected 6-chickens was medicated with 0.5%-futazolidone. The titer of premeditated birds was average 4.26 but after medication with furazolidone, the titers of treated 6-birds was average 4.08.
Investigation of respiratory disorders in slaughtered pigs
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 27~35
An abattoir survey of pneumonia and other lesions in slaughtered pigs from 5 selected herds located In the Western Kyongbuk was carried out during the period from March to December 1995. Pneumonic lungs was attempted bacteriological finding and antibiotic susceptibilities. From 583 slaughtered pigs, 445(76.3%) case was pneumonic lungs, seasonal patterns were Winter, Spring, Autumn, Summer in the order named. Among them, 127(21.8%) case was mycopla-sma pneumonia and 65(11.2%) case was pleuropneumonia. In snout lesion grade from 337 slaughtered pigs, above grade II score was 107(31.8%). In the white spot of liver, grade I was 544(93.3%), grade II32(5.5%) and grade III 7(1.2%). In the gastric ulcer, normal was 350(60.0%), grade I168(28.8%), grade II59(10.1%) and grade III 6(1.1%). Among the pigs(n=271) with pneumonic lesions above 20%, 162 strains werr isolated from 87(32.1%) pigs. The bacteria isolated from pneumonic lesions was Pasteurella sp 61(37.7%), Streptococcus sp 31(19.1%), Actinobacillus sp 3(1.9%), Coliform bacteria 19(11.7%) and the other bacteria 48(29.6%) These isolates were highly susceptible to the antibiotics including ENR 142(87.7%), Cft 138(85.2%) and Cf 126(77.8%).
Comparison of fatty acid composition of Staphylococcus sp isolated from bovine mastitis milk
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 37~45
The result of API staph-ident system was compared with cellular fatty acid composition for the identification of Staphylococcus species isolated from cattle. Isolated strains from cattle were correctly identified to S aureus, S intermedius, S hyicus, S simulans, S saprophyticus, S epidemis, S sciuri and S xylosus by API staph-ident system. The correlation between bacterial cellular fatty acid profile and Staphylococcus species isolated to API STAPH-IDENT system were. In conclusion, the result presented indicate that Staphylococci can be indentified to the species level by the cellular fatty acid profiles. Moreover, computerized fatty acid profile correlative anaylsis can be applied for determining identify of Staphylococcus species.
Prevalence of canine heartworm infection among dogs in Kwangju and Chonnam area
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 47~53
We have investigated the prevalence of canine heartworm (Dirofilaria immitis) infection among 100 mixed-breed dogs raised in Chonnam Province (Koheung, Posung, Changheung, and Hwasoon) and 100 dogs randomly selected from visiting dogs to the College of Veterinary Medicine, Chonnam National University for routine physical examination. Blood samples taken from dogs were examined for the presence of Dirofilaria immitis microfilaria by the modified Knott's test, and an antigen test which was based on the sandwich ELISA(DiroCHEK(equation omitted), Synbiotics, Inc., San Diego, California, USA) was used to detect the adult heartworm-specific antigen from serum samples. The results were then compared with those of the modified Knott's test. Five(2.5%) of the 200 examined dogs were microfilaria-positive, while 8 dogs(4%) were anti-gen-positive, which suggested that the antigen test was more sensitive than the microfilarial test in detecting heartworm infection. All dogs that were microfilaria-positive were also antigen-positive. The place of origin of the heartworm-positive dogs were of Naju (4/26, 15.4%), Koheung(3/32, 9.4%), and Hwasoon (1/19, 5.3%), while none of dogs raised in Kwangju, Posung, and Changheung areas were infected with the parasite. Although not significant, more infected dogs were observed in older ages, while the difference in infection rate between male (4/98, 4.1%) and female (4/102, 3.9%) was not recognized. Our results indicated that a relatively low infection rate was observed in dogs raised in Kwangju and Chonnam area compared to the infection rate reported previously in other areas of South Korea.
Development on the lungs of fetuses and neonates in Korean native goats
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 55~67
The morphological development of lungs in fetuses of 60, 90 and 120 days of gestation and neonates of Korean native goats was investigated by light, scanning and transmission electron mictroscope. The results were summarized as follows ; 1. Gross findings ; In the 60-days-old fetus, the lung was developed and differentiated into six lobes. 2. Light microscopic findings : The gland-like bronchioles were formed in loose mesenchyme at 60 days of gestation and the bronchial wall contained smooth muscles. The loose mesenchyme had been replaced by compact parenchymal tissue at 90 days of gestation and the cartilage plates appeared in bronchial wall which contained blood vessels, submucosal glands and smooth muscles. The lung parenchyma consisted of a fine network of alveoli at 120 days of gestation and the bronchial wall contained well-developed blood vessels, submucosal glands, cartilage plates and smooth muscles. In neonates, the lung tissue was similar to the mature lung tissue and the bronchial wall contained well developed cartilage plates. 3. Scanning electron microscopic findings : The epithelial cells lining the tubules were composed of cuboidal or columnar at 60 days of gestation and the epithelial cells lining the large airways were often ciliated : some were covered with stubby microvilli. The epithelial cells lining the canals were cuboidal at 90 days of gestation and the epithelial cells lining the bronchioles were ciliated cells or nonciliated(clara) cells, The clara cells contained row microvilli. The alvealor development of this stage was rapidly progressed ; the subdivision of canals by alveolar crests and assosiated wall attenuation resulted alveoli at 120 days of gestation and the respiratory bronchioles were lined by ciliated or nonciliated epithelial cells. In neonates, the epithelial cells lining the alveolar walls were mainly covered with pneumocyte type I ; Some were covered with pneu-mocyte type II. 4. Transmission electron microscopic findings : The epithelial cells lining the tubules were adhered with tight junction at apical borders of the adjacent cells at 60 days of gestation, which contained few organells and glycogen. The epithelial cells lining the canals were composed mostly of cuboidal cell at 90 days of gestation and the epithelial cells lining of the bronchioles were ciliated of nonciliated cell, which contained few organelles and abundant glycogen. The epithelial cells lining the alveolar walls were composed of pneumocyte type I and a few pneumocyte type II at 120 days of gestation. The epithelial cells lining of the bronchioles were ciliated or nonciliated cells. In neonates, pneumocyte type I was observed as flat and thin cytoplasmic extension in shape. Otherwise, pneumocyte type II was observed as cuboidal type with apical microvilli and contained osmiophillic lamellar inclusion bodies. Putting these various experiment results together, the lung development was slowly progressed at early stage, which was rapidly progressed in the late stage of gestation.
Isolation of Listeria monocytogenes from animal carcasses and environmental specimens in slaughter house level
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 69~77
To invastigate the epidemiological trait of listeriosis, Listeria monocytogenes were isolated from the carcasses of pigs and cattle, and environmental specimens in slaughter house. Also serotype of isolates were classified by rapid slide agglutination test. In the carcasses of pigs, Listeria sp were isolated from the carcasses after bleeding(62%), after dismemberment(60.0%) and before shipping(76.0%), and L monocytogenes were present in 8% of the carcasses after dismemberment and in 14% of the carcasses before shipping. However, few Listeria sp were isolated from the living body skin and the carcasses after scalding. In the carcasses of cattle, Listeria sp were isolated from the carcasses after bleeding(10%), after dismemberment(36.7%) and before shipping(63.3%), L monocytogenes were present in 3.3% of the carcasses after dismemberment and in 10% of the carcasses before shipping. Overall, L monocytogenes, L innocua, L welshimen, L grayi, and L murrayi were present In 4.8, 40, 2.3, 2.6 and 0.3% of all the carcasses, respectively. Prevalence of Listeria sp in environmental specimens were found to be 80% in slaughter house floors and 100% in sewage, and L monocytogenes were present in 15% of sewage. However, few Listeria sp were isolated from chilled water and from scalding water. Overall, L monocytogenes, L innocua, and L welshimeri were present in 3.8, 45 and 6.3% of all the environmental specimens, respectively. A total 27 strains of L monocytogenes were isolated from samples tested and all of the strains were classified into serotype 1.
An experimental study on the comparison of trace amount of sulfonamides detection method in raw milk.
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 79~93
The sulfonamide is one of potentiative antimicrobial agents which is being used widely in veterinary medicine for control of several animal diseases such as mastitis as well as for promotion of growth. However, the misusages of sulfonamides in food producing animals, especially cattle produce several considerable problems in human health caused from residues of this antibiotic in milk product. To determine the most effective analytical methods for residual sulfonamides in raw milk, this study was performed comparatively using by some applicable screening detecting method such as TTC, Charm II test (sulfonamides), and Lactek tests (sulfamethazine kit). The positive result from screening tests was confirmed by HPLC method. Milk samples (540 raw milks) were collected from dairy farms. Results of this study are summariezed as follorrs ; 1. All samples (540 raw milks) showed negative response from TTC test, however, 18 raw milks of those samples responded positively to Charm II test. 2. By Lactek test, residual sulfamethazine was detected from 4 raw milks. Fifteen raw milks of 18 samples which were classified as positive one by Charm II test, showed positive response 3. Retention time of sulfonamides added at the level of 100ppb into sklm milk was ranged from 1.55 minute to 23.3 minute. Recovery rates of sulfonamides were variable from 6.7% upto 94.2% depended on the types of sulfonamlde. 4. Single type of sulfonamides was detected from 10 raw milk samples, 2 types of sulfonamides from 3 samples and 3 types from 2 raw milks by HPLC. 5. Sulfonamides was detected in this study were 5 types : 11 samples for sulfisomidine, 5 samples for sulfamethazine, 3 samples for sulfadlmethoxine, 2 samples for sulfathiazole and 1 sample for sulfadiazine. 6. The highest levels of residual sulfonamides was 210.3 ppb of sulfamethazine but the lowest concentration of residue was 2.2 ppb of sulfamethazine and sulfisomidine, respectively. Number of samples detected positively in this experiment were belows : above 100 ppb for 1 sample (4.5%) (sulfamethazine), 50~100 ppb for 4 samples (18.1%) (each 2 samples for sulfamethazine and sulfisomidine, respectively), 25~50 ppb for 6 samples (27.1%) (2 sulfisomidine, each 1 sample for sulfadiazine, sulfadimethoxine, sulfamethazine and sulfathiazole, respectively), 10~25ppb for 3 samples (13.7%) (3 sulfisomidine), and below 10ppb for 8 samples (36.4%) (4 sulfisomidine, 2 sulfadimethoxine and each 1 for sulfamethazine and sulfathiazole).
A survey on the prevalence of internal parasitism in dog of Inchon area.
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 95~102
Author investigated internal parasitism for the feces of dog's training center, breeding-dog farm, dairy farm, home by 120 indoor breeding dog and 566 outdoor one in Inchon area. This survey was done from February in 1994 to December in 1995. 1. As a result of total 686 samples, positives were 373(54.4%). Among them, indoor and outdoor breeding dogs were 21(3.1%) 352(51.3%), respectively. 2. According to breeding, it was manifested that 21samples (17.5%) of 120 indoor breeding dogs were positive, and 352 samples(62.2%) of outdoor breeding were positive. 3. The infection rate of dogs for food in dairy farm, breeding dogs in the farm, dog of training center and dog of house is high in order. 4. Infection rate of parasites in 24 dogs breeds, Mongrel dogs were 81.3%, Shepherds were 80.0%, Tosas were 78.4%, Akida and Siberian huskys were 76.2%, Jindos were 55.5%, Pointers were 50.0%, although Afghan hound, Spanial, Shin-tzu, Maltis and Buldog were examined as aparasites negative. 5. After administration with vermicide parasites infection rate were 43.0% in two months. In four months, it were 66.7%, and dogs without vermicide were 87.0%. It seemed like that further research about dosage of vermicide is needed. 6. The rate of single-infection was 37.6% and that of mixed-infection was 16.8%. Among classified 13 types, Ancylostoma caninum 35.6%, Toxocara cams 11.2%, Isospora sp 9.3%, Toxascaris leonina 5.1%, Trychuris vulpis 4.4% were investigated.
Studies on biochemical and immunological properties of Aetinobacillus pleuropneumoniae Isolated from the slaughter pigs with respiratory lesions in chungnam province
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 103~125
An epidemiologic study on pleuropneumonia in the slaughter pigs(Chonan and Asan area, Chungnam province, Korea) during the period of January 1994 through December 1995 was conducted. Isolation of A pleuropneumoniae was attempted in 425 pigs with pneumonic lesions. Biochemical properties, antimicrobial susceptibility, serotypes and pathogenicity of isolated A pleuropneumoniae were investigated. In addition, outer membrane protein(OMP) of the Isolates were extracted to determine its properties and immunogenicity in both mice and piglets The results obtained through this study were summarized as followed ; 1. Of 3, 395 slaughter pigs, pleuropneumonia was observed in 425 pigs(10.6%). A pleuropneumoniae was isolated from 22 pigs(5.2%) out of 425 pigs with pneumonic lesions. The biochemical properties of all isolates were same as those of reference A pleuropneumoniae strain. Among 22 isolates, 9, 1 and 12 isolates were serovar 2, 3 and 5, respectively. 2. The results of antimicrobial susceptibility test revealed that the isolates showed high susceptibility to ciprofloxacin and cephalothin, moderate susceptibility to amikacin, gentamicin, kanamycin and streptomycin, and low susceptibility to erythromycin, tylosin and sulfadimethoxin. 3. The isolates were varied in pathogenicity to mice. Median lethal dose of LE9402(serovar 2) and LE9511(serovar 5) were
CFU, respectively. Specific pneumonic lesions were observed from the infected mice with clinical signs. Bacteria recovery rate was high in the lung, but low In heart blood and tracheas. 4. Serovar 2 was found to be more pathogenic than serovar 5 in guinea pig. Mortality on guinea pigs inoculated with serovar 2(
CFU) and serovar 5(
CFU) was 20~40% and 40~80%, respectively. A severe hemorrhagic lesions and focal pneumonic lesions were observed from dead guinea pigs. Bacteria recovery rate was relatively higher in the lung than that of other organs. 5. In the SDS-PAGE analysis, OMP-enriched fractions of both isolates and reference strains contain common peptide bands equivalent to molecular weight of 17, 27, 42, 52 and 95Kd. In addition to common peptide bands, the bands which are specific to each isolate were also observed. The profiles of Sephadex G25 fractions showed 3 major peaks. The common peptide bands which were observed by SDS-PAGE of the crude OMPs were found in the peaks 1 and 2. 6. The OMPs extracted from serovar 2(LE9402) and serovar 5(LE9511) provided high level of protection in mice(70~80%) and pigs(100%). All animals inoculated with OMPs were seroconverted, showing micro-agglutination titer of 640 to 1280.
Serum antibody titer test on the deformity of cattle
Korean Journal of Veterinary Service, volume 20, issue 1, 1997, Pages 127~129
The cattle serum was investigated many cases of deformity calf in kyung-ju area. The serum antibody titer of Aino virus, which was not reported in domestic, was showed 3 cattle to