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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 1999
Volume 22, Issue 3 - Oct 1999
Volume 22, Issue 2 - May 1999
Volume 22, Issue 1 - Apr 1999
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Study on etiological agents of sudden death in cattle at the region of Sarari in Korea
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 325~347
This study was conducted to investigate the epidemiological, clinicopathological, microbiological, pathological observations and other tests from sudden death in feedlot cattle at the region of Sarari in Korea during the period from 1994 to 1999. Massive or sporadic occurrence of sudden death has been observed in 101 heads of 47 farmhouse. There were 20.8% in spring, 29.7% in summer, 16.8% in autumn, 32.7% in winter, and 62.3% in reproductive, 27.7% in growing, 5.0% in beef cattle, 5.0% in calf in prevalence of sudden death in cattle. Enterotoxemia(88.0%), pneumonia(3.5%), intestinal diarrhea(3.5%), liver abscess(1.5%) and indigestion(1.5%) were detected from 67 heads of sudden death cattle. In clinical observations, cattle were generally died of sudden recumbency with convulsions followed anorexia, depression, ataxia, muscular tremor, tachycardia and dyspnea without any premonitory symptoms. Epidemiological surveys showed no evidence that other factors such as pesticide, insecticide, fertilizer, chemical drug3 and those of others caused sudden death. Macroscopically, there were coagulation disorders of blood, congestion, edema and haemorrhage of lung, congestion and haemorrhages, watery and blood-tinged contents of small intestine. Histopathologically, we observed pulmonary congestion and haemorrhage, necrotic intestinal mucosa accompanied with haemorrhage and congestion, and also increased globule leukocytes between bronchial epithelia with mild pneumonia. Clinicopathologically, only elevation of blood glucose and aspartate aminotransferase(AST) was detected. Magnesium and calcium deficiency were not detected, but parasites were detected highly in normal and dead cattles. Microbiologically, Clostridium(Cl) pefringens were detected from small intestinal contents of 94% (63/67) of sudden death cattle and 51%(51/101) of slaughter cattle, and the population were
after 16~32 hours. Consequently, it was proved that the cause of death in cattle was enterotoxemia. Pathogenic test of mouse and goat inoculated with Cl perfringens type A toxin has been demonstrated as similar observation to natural cases. In antimicrobial susceptibility test, ampicillin, bacitracin, polymycin, cephalothin, penicillin, choramphenicol, erythromycin, tetracycline were highly susceptible, and amikacin, gentamicin, kanamycin, neomycin, streptomycin, sulfamethoxine, sulfamethazine were resistant. Cl perfringens were resisted for 4 hours in 3% formalin, 20 minutes in 4% phenol, 20 minutes in 0.5% mercuric chloride and 40 minutes in 0.1% sodium hydroxide, respectively. The useful method to prevent from occurrance of enterotoxemia in feedlot cattle was a dietary administration of antibiotics and miyari acid.
A case report of clostridium botulinum in cattle
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 349~356
Type C botulism was broken out in cattle at Pochun country, Kyonggi in Korea. The main cause was on the feed of fermented food contaminated with Clostridium botulinum. It was supposed to be contaminated with C botulinum during producing the fermented food because of either using contaminated food residues or adding contaminated additive materials like sausage. One hundred and twenty out of 642 cattle in 11 farms showed typical botulism symptoms and 95 among them died. The incubation period was from 2 to 14 days and the average incubation period was 8 days. The mortality in this case was 18.3%.
The study for the effect of biofilter and ultra-violet disinfector in fish-breeding place using seawater
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 357~361
The most harmful nitrogenous compounds in fish-breeding place using are ammonia and nitrate. Excessively high total nitrogen concentration is the signal of unbalance for breeding fishes in seawater and may result overfeeding or overstocking without seawater treatment system. The failure of elimination for the organic ingredients or nitrogen compounds can also cause the consequence of inadequate oxygen concentration in seawater, either. The study shows the effect of biofilter and W disinfector of seawater in the fish- breeding place. In the results, these tools had ammonia, nitrite, nitrate and decreased 71.8%, 27.6% and 1%, respectively, and the total number of microorganisms decreased up to 81.9%.
Isolation and identification of porcine reproductive and respiratory syndrome virus from serum samples collected from swine farms
Kim, Hyun-Soo ; Kong, Sin-Koog ;
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 363~370
Isolation of PRRSV was attempted from 646 swine sera collected from swine farms. The MARC-145 cell, which is highly permissive to PRRSV, was used for virus isolation, propagation, IFA test, and VN test. Total 36 cytopathic viruses to MARC-145 cells were isolated. The virus isolates were identified as a PRRSV by the IFA test and VN test using the reference sera prepared by experimental infection of reference PRRSV CNV-1 into 30 day-old pig. In addition to serological conformation, ORF5 of genomic RNA of 6 selected cytopathic viruses were amplified by the RT-PCR. The resulting PCR products were examined by electrophoresis in 1.2% agarose gel. An appropriate bands of about 680bp including the flanking sequence of total 80bp were seen on agarose gel.
Detection of antibody to porcine reproductive and respiratory syndrome virus from pig sera collected from pig farms
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 371~375
Total 1,434 sera collected from 72 pig farms were tested for the detection of porcine reproductive and respiratory syndrome (PRRS) virus antibodies. The overall seroprevalence of PRRS virus antibodies was 49.3% (707/727). Of 72 farms tested 59 (81.9%) farms had at least one or more than one pigs with PRRS virus antibodies. The seroprevalence of PRRS virus antibody varied with age. Seroprevalence of PRRS virus antibody in 1 to 30-day-old, 31 to 40-day-old, 41 to 50-day-old, 51 to 60-day-old, and over 61-day-old pig were 27.4%, 52.3%, 57.9%, 52.7%, and 68.2%, respectively. Gilt showed relatively higher seroprevalence (61.2%) than sow (29.2%) and boar (38.3%). In most farms, the infection of PRRS virus was chronic and confined to grower or finisher. This pattern of infection suggests that partial depopulation of the infected herds appears be one of the measures to eradicate the PRRS virus infection. High seroprevalence of the PRRS virus antibody in gilts and boars indicates that the infected gilts and boars in the breeding farms are the major source of the PRRS virus infection, and also play an important role in spreading the PRRS virus between fan mates or herds.
Functional studies of granulocytes in ruminants 1. Rapid separation of polymorphonuclear leucocytes from circulating blood in bovine
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 377~383
Polymorphonuclear (PMN) leucocytes are fundamental importance to the body's defense mechanism and play a major role in the local and systemic reactions to infectious disease. Investigation of the physiological and pathological role of the various leucocyte subtypes in host defence mechanisms is dependent upon the isolation of adequate numbers of viable, pure leucocyte fractions. This report describes the separate frequency of PMN leucocytes both from buffy coat layer and from packed RBC layer when bovine peripheral blood was treated with various anti-coagulants such as acid-citrate-dextrose(ACD), ethyldiaminetetraacetic acid(EDTA), sodium citrate and heparin. The separate frequencies of PMN leucocytes from buffy coat layer was 60.4
11.8%(sodium citrate), 30.6
14.1%(ACD) and 6.2
3.7%(EDTA), in order. Those from packed RBC layer monitored with EDTA, ACD, sodium citrate and heparin was 85.0
6.5% and 76.3
7.7%, respectively. The Ficoll-hypaque(FH) density gradient method was used to remove a small part of lymphocytes and/or monocytes from leucocytes in packed RBC layer. With the result that it increased separate frequency of PMN leucocytes from EDTA(89.9
3.6%), and sodium citrate(83.6
10.3%) than heparin(68.4
13.9%). These results indicate that the use of EDTA and ACD as anticoagulant Is suitable for the separation of PMN leucocytes from bovine peripheral blood, and that the FH density gradient method is able to increase the separate frequency of PMN leucocytes from packed RBC layer.
Serological studies of avian rotaviruses in chicken sera using indirect immunofluorescence assay
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 385~392
A total of 420 chicken sera from various regions were tested for the presence of antibodies to avian rotavirus using indirect immunofluorescence assay (IFA). In broiler farms, rotavirus antibodies were detected from 20 farms among 30 farms tested and the positive rates were above 50% in 9 farms. In parent stock farms, rotavirus antibodies were detected from 5 farms among 14 farms tested. From sera collected in 7 layer farms rotavirus antibodies were not detected.
Acute carbohydrate engorgement in the Korean indigenous cattle, a case report
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 393~398
This case occurred following accidental consumptions of toxic levels of feedstuffs in the Korean indigenous cattle which were pregnant for 3-6 months. Grossly, poorly defined bluish coloration in the ventral sac of the rumen, reticulum and omasum was visible on the surface of the stomach. The severely affected ruminal walls were perforated and blood-tinged exudate was observed and death followed due to acute diffuse peritonitis. There were copious amount of porridgelike rumen contents, which has a distinct fermentative odor. The epithelia were easily wiped off and hyperemic in patch. Histopathologically, chemical rumenitis with neutrophilic infiltration and opportunistic fungal infection was evident in the mucosa and submucosa. Multifocal necrotic foci with fungal hyphae on the liver and severe congestion on the renal medulla were noted. There was no evidence of heavy metal and aflatoxin Bl in the suspected feedstuffs. This case was diagnosed as acute carbohydate engorgement in cattle.
A survey on the prevalence of parasites in quail of Chonbuk area
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 399~404
In order to monitor the parasites, 635 fecal samples were taken from quail in Chonbuk area. The isolation and identification of the parasites were determined by the fecal examination using the floatation and microscopical examination, respectively. The detection rate of the eggs and unsporulated oocysts from the intestine and feces were 37.8%(240 heads). In the concerns of complicated infection, infection with single, double and triple were 43.4%(217 heads), 3.4%(22 heads) and 0.1%(1 heads), respectively. The parasites isolated were identified as eimeria spp from 205 heads, strongyloides avium from 36 heads and demanyssus spp from 23 heads.
Histopathological study of uremia in a juvenile shepherd dog
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 405~410
A 7 month old male shepherd was presented with anorexia, diarrhea, and salivation, and euthanized due to no clinical improvement. Grossly, ulcers were seen on ventral surface of tongue and stomach. The mineralization was obvious in the intercostal space, lung and inner surface of aorta. Kidney was pale, firm and irregular. Histopathology confirmed uremic pneumonitis, end stage kidney and hyperplasia of parathyroid. This case was the typical uremic condition of juvenile dog, which was characterized the diffuse soft tissue mineralization due to hyperparathyroidism secondary to chronic renal failure.
Efficacy of chlorine and lactic acid for reducing pathogenic and spoilage microorganisms on chicken skin
Korean Journal of Veterinary Service, volume 22, issue 4, 1999, Pages 411~418
In this studies, the ability of chlorine and lactic acid to reduce bacterial population of the pathogenic microorganisms were examined on artificially inoculated chicken skin. About 10
cells of staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and escherichia coli O157:H7 were inoculated in chicken skin. The contaminated samples were washed for 1 min with sodium hypochlorite solutions that contained 2, 5, 10, 20 and 50mg/
available chlorine and counted number of the agents. Viable population were no significantly difference (p
0.05) between concentration of chlorine and strains of the pathogens. In the samples inoculated with pathogens were washed in 20mg/
chlorine and then stored at
for up to 10 days, the initial counts of psychrotrophs and aerobic plate counts were 4.02 to 4.36 log cfu/
and increased slightly in course of time. But 10 days after, the pathogens were a little reduced from 3.66~4.91 log cfu/
to 2.54~4.66 log cfu/
. In the case of washed skin with solution of 20mg/
chlorine and 0.5% lactic acid then store at
for up to 10 days, population of psychrotrophs and aerobic plate counts on chicken skin were markedly reduced immediately after treatment, but the numbers of contaminants were slightly increased after 6 and 8 days. Specifically, numbers of St aureus, S enteritidis, L monocytogenes and E coli O157:H7 were reduced to 0.5, 0.4, 0.3 and 1.15 log cfu/
after 10 days of storage, respectively, on aerobic plate counts.