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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 24, Issue 4 - Dec 2001
Volume 24, Issue 3 - Oct 2001
Volume 24, Issue 2 - Jun 2001
Volume 24, Issue 1 - Apr 2001
Selecting the target year
Prevalence of Brachyspira hyodysenteriae on selected swine farms in Gyeongbuk province by PCR
Suh, Dong-Kyun ; Do, Yun-Jeong ; Ha, Jong-Su ; Lee, Kyeong-Hyeon ; Cho, Yoo-Jung ; Song, Dong-Jun ; Lee, Chun-Sik ; Bae, Young-Chan ; Park, Won-Pil ; Lee, Keun-Woo ; Song, Jae-Chan ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 331~334
The purpose of this study is to determine the prevalence of Brachyspira hyodysenteriae from 43 swine farms with diarrhea or a history of diarrhea in Gyeongbuk province by PCR. The overall herd prevalence of B hyodysenteriae was 37.2%(16/43), and the average prevalence of B hyodysenteriae among all sampled pigs was 10.8%(50/462). Positive herds for B hyodysenteriae were distributed throughout Gyeongbuk province from 14.3～50%. Herd size had a effect on the frequency of B hyodysenteriae. The frequency of B hyodysenteriae in herds with less than 1,000 pigs was 47.4%, that of herds between 1,001 and 2,000 pigs was 41.7%. Also, the frequency of B hyodysenteriae in herds with more than 2,000 pigs was 16.7%.
Rapid detection of Brachyspira hyodysenteriae in swine intestinal specimens by PCR
Dong-Kyun Suh ; Yun-Jeong Do ; Jong-Su Ha ; Kyeong-Hyeon Lee ; Dong-Jun Song ; Chun-Sik Lee ; Young-Chan Bae ; Suk-Chan Jung ; Won-Pil Choi ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 335~341
Swine dysentery caused by Brachyspira hyodysenteriae, an anaerobic, beta-hemolytic spirochete, is a severe mucohemorrhahic diarrheal disease that primarily affects pigs during the growing and finishing period. The current standard laboratory procedure to culture and identify B hyodysenteriae takes 3 to 7 days. This report present a rapid PCR for detection B hyodysenteriae in a single reaction using DNA from swine intestinal samples. The PCR produced a specific 421bp PCR product with template DNA purified from B hyodysenteriae, and the accuracy for detection of B hyodysenteriae by PCR results compared with those of conventional method was 100% in intestinal specimens. Nonspecific bands were not detected with B innocens, a nonpathogenic common inhabitant spirochete, including other enteric bacterial organisms. This procedure could detect as little as 50 pg of template DNA for B hyodysenteriae.
Detection of antibody to porcine reproductive and respiratory syndrome virus from pig sera collected during the period of January to December 2000
Jung, Hae-Sun ; Kim, Su-Mi ; Kim, Jong-Taik ; Han, Tae-Uk ; Kang, Shien-Young ; Shin, Kwang-Soon ; Kim, Chul-Joong ; Park, Bae-Keun ; Kim, Hyun-Soo ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 343~346
During the period of January to December 2000, a total of 3,505 swine sera was collected from 208 farms, which are located throughout country, for the diagnosis of porcine reproductive and respiratory syndrome(PRRS). The antibody to porcine reproductive and respiratory syndrome virus(PRRS) was tested by indirect immunofluorescent antibody(IFA) test. Of 208 farms tested, at least one or more than one pigs was positive for PRRSV antibody in 188(90.4%) farms. The overall seroprevalence of PRRSV antibody was 45.1% (1581/3505). Most pigs were infected with PRRSV at around 50- to 60-day old. The seroprevalence of antibody varied with age. The highest seroprevalence of PRRSV antibody was observed in the growing pigs at around 80-day old. About one-thirds of adult pigs including boar, gilt and sow were positive to PRRSV antibody. In many farms, the infection of PRRSV was chronic and confined to grower and/or finisher. However, antibody was detected from all production phase in some farms.
A survey on the incidence rate of foot diseases in dairy cattle
Lee, Cheong-San ; Ryu, Dae-Yeol ; Kwak, Hak-Koo ; Hyun, Gong-Yul ; Park, Kyung-Jae ; Cho, Woo-Young ; Lee, Jong-In ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 347~352
This study was carried out to investigate the status of hoof diseases and to develope treatment method for cattle with hoof diseases during the period of January to December in 2000. Out of 435 heads, 34 heads(7.8%) had hoof diseases. In respect to season, incidence rate was higher in August to September than that of other seasons. The incidence rates of hoof rot, pododermatitis verrucosae, laminitis, other cases and trauma were 14 cases(41％), 10 cases (29%), 7 cases(21%), 2 cases(6%) and 1 case(3％), respectively. In respect to age and milk production, Incidence rate was higher in the cattle with high milk production. Incidence rate of hoof diseases on the hind-limbs was higher than that of the fore-limbs. E. coli(8 strains), Clostridium spp(8 strains), Staphylococcus spp(6 strains), Fusobacterium spp(6 strains), and Bacteroides spp(6 strains) were isolated from the hoof lesions. All isolates were sensitive to composite preparation made of mainly formalin with picric acid and phenol. (Treatment against each strains isolated was sensitive to composite preparation made out of the main constitutions of formalin with picric acid and phenol).
Survey on the sorbic acid concentrations in cooked meat produced from meat processing plants
Do, Jae-Cheul ; Cho, Jong-Suk ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 353~358
Sorbic acid is one of preservatives that is mostly used in general food. This study was carried out to measure the amount of the sorbic acid in cooked meat produced from meat processing plants in Gyeongbuk province. One hundred and fifty-three samples of cooked meat(42 spices added meats, 56 grinding cooked meats, 2 bacons, 5 jerked meats, 18 sausages, 30 hams) were collected from meat processing plants and analyzed for the concentration of preservative sorbic acid and its salts by using of high performance liquid chromatography(HPLC). Sorbic acid was not detected in spices added meats, grinding cooked meats, bacons, and jerked meats except on ham and sausage. But the concentration range of sorbic acid in sausage was 0.00～1.31g/kg, average 0.44g/kg, and in ham was 0.00～1.31g/kg, average 0.329/kg. There was no sample that sorbate concentration exceeded the legal permitted level 2.0g/kg in cooked meat.
Serologlcal survey of infections agents in domesticated boars
Cho, Kwang-Hyun ; Park, In-Hwa ; Kim, Young-Hoan ; Kim, Soon-Tae ; Kim, Sung-Kook ; Park, No-Chan ; Son, Jae-Kwon ; Jyeong, Jong-Sik ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 359~367
A serological survey was performed to establish basic data for the prevalence of antibodies to some major diseases of domesticated boar serum samples from January to December 2000. Sera collected in breeding farms in Gyeongbuk province were tested for Aujeszky's disease virus(ADV), Porcine reproductive and respiratory syndrome virus(PRRSV), Porcine parvovirus(PPV), Japanese encephalitis virus (JEV), Bordetella bronchiseptica(B bronchiseptica), Mycoplasma ; APP), Toxoplasma, and Brucella. There was no antibody to ADV in domesticated boars serum samples detected by Anti-ADV-gpI assay kit. Sero-positive samples to PRRS by IFA were 0.9%(3/330) The HI titers to PPV ranged variously from less than 10 to over 1,280. Two hundred ninety-four out of 330 tested sera showed HI titer of less than 10. In HI test to JEV, 90.3% of the sera (298/330) were below 10. The majority of the serum samples had low prevalence of the antibody B bronchiseptica. ELISA titers to M hyopneumoniae ranged variously from
1,280. Antibody titers to A pleuropneumoniae type 2(APP2) and type 5(APP5) were 58.2% and 52.7%, respectively, and the tested samples showing ELISA antibody titers of less than 20. There was no significant geographical difference between APP2 and APP5 in this study. In the antibody test of Toxoplasma, 11.5%(38/330) were positive and samples were all negative in sera test of Brucella.
Observations of the incubation of imported ostrich (Struthio camulus) eggs in a farm
Park, Su-Youn ; Lee, Hee-Mun ; Kiku Matsuda ; Lim, Chae-Woong ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 369~374
This study was conducted to investigate the hatchability and infertility of the ostrich eggs. Seven batches of ostrich eggs were imported from Australia into Korea in winter season under quarantine restrictions. Single stage incubation was carried out and the eggs were weighed before incubation. The weight of imported ostrich eggs was varied from 1,074 to over 1,650g and the average egg weights for batches were similar. The hatchability of all eggs batch was reached between 11 and 31%. The infertility of seven batches was varied from 36 to 63%. The first batch of eggs showed relatively low infertility(39％) and high hatchability (31％). On the contrary, the last batch of eggs had low Infertility(36%), and it had the lowest hatchability(11%). The result of this study suggests that hatchability of ostrich eggs imported in late laying season is significantly low because the number of infertile eggs is increased.
Rectal stricture in a finishing swine : Case
Seol, Min-Sug ; Hur, Boo-Hong ; Kiku Matsuda ; Lim, Chae-Woong ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 375~377
Rectal stricture occurred in 2 finishing pigs submitted for necropsy from Moguchon, the meat processing plant, chonbuk. Grossly, the wall of the rectum was harden and thickened by fibrous tissue. Anteriro to the stricture, the descending colon was dilated up to 30cm in diameter, filled with gas and pasty green fluidal feces. Histologically, the epithelia of rectal mucosa were necrotized. The mucosa and submucosa of rectum were infiltrated by macrophages, eosinophils and lymphocytes. This infiltration was the most extensive in the deeper layer of submucosa and intensive fibrosis was observed in deeper submucosa layer. This case is report for rectal stricture of finishing pig.
Immunization of broiler chicks deprived food and water with live Newcastle disease vaccine(LaSota strain) by drinking water
Kwak, Kil-Han ; Seo, Suk-Yul ; Park, In-Bang ; Ryu, Kyeong-Seon ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 24, issue 4, 2001, Pages 379~382
To confirm the effect of food and water deprivation prior to Newcastle disease(ND) virus vaccination, three hundred chicks were divided into five groups with three replications. ND vaccine were sprayed to at 1 -day old chicks at commercial hatchery. Secondary and third vaccination was conducted at 2-week old and 24-day old chicks by LaSota strain. Control was conventionally vaccinated without withdrawing the food and water before or after vaccination. In group 2(G2) and 3(G3), LaSota strain was vaccinated to chicks before and after fasting the food and water for 3 and 2 hours, respectively. Group 4(G4) has the same fasting time of group 2, but supplemented the skim milk in vaccin dilution water. In group 5(G5), skim milk was added into group 3. Weight gain, feed intake and feed conversion were weekly measured for 5 weeks. Blood was collected from wing vein at 24 and 35 days of age. Each serum antibody level were measured by hemagglutination inhibition(HI) test. The average weight gain, feed intake, feed conversion of all group were not significantly different. Weight gain of each groups was 1910.30(control), 1875.28(G2), 1952.12(G4) and 1896.05(G5), respectively. Feed intake of all group was recorded at 3160.67(control), 3167.07(G2), 3189.48(G3), 3157.85(G4) and 3178.16(G5), respectively. The feed conversion of each groups was 1.655(control), 1.688(G2), 1.633(G3), 1.699(G4) and 1.676(G5), respectively. The HI titer of G4 was
and significantly higher than the other groups (p<0.05)(control :
, G2 :
, G3 :
, G :
at 35 days old. The results of this experiment indicated that two or three hours of fasting time before or after vaccination would be able to show the higher antibody level against ND virus.