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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 29, Issue 4 - Dec 2006
Volume 29, Issue 3 - Sep 2006
Volume 29, Issue 2 - Jun 2006
Volume 29, Issue 1 - Mar 2006
Selecting the target year
Application of PCR for diagnosis of porcine circovirus type 2
Park Hyo-Sun ; Lee Hyo-Sang ; Na Ki-Bok ; Lee Kwan-Bok ; Kang Su-Jung ; Moon Sun-Hwa ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 1~8
Porcine circovirus (PCV) is a small, nonenveloped virus that contains a single-stranded circular DNA genome of about 1.76 kb and belongs to the family circoviridae. The PCV-2 has been incriminated as the cause of post-weaning multisystemic wasting syndrome (PMWS) , an emerging disease in pigs. In the present study, a PCR assay was applied to detect PCV-2 in tissue samples. The presence of PCV-2 antigen in the porcine tissues was confirmed by indirect immunofluorescence (IIF) with PCV-2 specific monoclonal antibodies. And then DNA extracted from PCV-2 positive tissues was used as a template. One oligonucleotide primer suitable for PCR was selected from a published PCV-2 sequence (Genbank). Amplified PCR product was detected the same fragment lengths of 416 bp as a control. Based on these results, it was suggested that the PCR is a simple and sensitive method for support diagnostic purposes.
Serological survey of avian pneumovirus and reovirus in breeders of Jeonbuk province
Lee Jeoung-Won ; Shon Ku-Rye ; Park Ki-Shung ; Kim Youn-Tae ; Kim Choo-Cheol ; Han Kyu-Sam ; Lee Hee-Mum ; Song Hee-Jong ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 9~18
The ELISA titers to avian pneumovirus (APV) and avian reovirus (ARV) were surveyed to sera of 38 breeder farms (78 blocks, 1,560 hens) in Jeonbuk province during February to October, 2005. In APV, the positive ELISA were detected 36 (94.7%) breeders, 71 (91.0%) blocks, 1,057 (67.8%) hens, and their S/P ratio was 1.940. Regionally, the positivity of 24 breeders farms in the Jeonju, Jeongeup, Namwon, and Jangsu were noted as 100%, whereas 85.7% in Iksan. The positivity to species such as Cobb (20), Ross (13) and Hanhyup-3 (5), all of the breeding farms were detected as positive, 40 (86.9%), 17 (94.4%) and 14 (100.0%) in blocks, 553 (60.1%), 285 (79.2%) and 219 (78.2%) in hens, and their mean S/P ratio were 1.677, 1.769 and 2.254, respectively. The positivity of the breeders vaccinated with ARV, all of the 9 breeder farms (38 blocks) were noted as 100%, but 627 (82.5%) in hens, and its mean S/P ratio was 1.273. Whereas nonvaccinated with ARV were 28 (96.5%) in breeders farms, 38 (95.0%) in blocks, 660 (82.5%) in hens, and the mean S/P ratio was 1.612. In species which were vaccinated with ARV, 11 breeder farms (38 blocks) were noted as 100%, but 82.5% in hens, and their mean S/P ratio were 1.315. Whereas in nonvaccinated with ARV, 25 (92.6%) in farms, 38 (95.0%) in blocks and 660 (82.5%) in hens were positive, and their mean S/P ratio was 1.532.
The minimum inhibitory concentration (MIC) of bee venom against bacteria isolated from pigs and chickens
Kim Soon-Tae ; Hwang Ji-Young ; Sung Myoung-Suk ; Je So-Yang ; Bae Dong-Rok ; Han Sang-Mi ; Lee Sung-Hae ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 19~26
Bee venom is synthesized in the venom gland's of worker and queen bees and stored in their venom sacs. Bee venom is a rich source of enzymes, peptides and biogenic amines. there are at least 18 active components in the venom which have some pharmaceutical properties. This study was performed to evaluate minimum inhibitory concentration(MIC) of bee venom against bacteria isolated from pjgs and chickens with disease. In case of reference strains, the MIC
of Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, and Salmonella typhimurium ATCC 6538 were 64, 64 and 32, respectively. In case of bacteria isolated from pig and chicken, the MIC of Staphylococcus aureus, Staphylococcus hyicus and Staphylococcus chromogenes were 8, 128 and 128, and that of 11 strains of Escherichia coli were 8 to >512 and that of 8 strains of Salmonella sup were >512. Antibacterial resistance test of 22 strains isolated from pig and chicken and 3 reference strains were performed by agar gel diffusion method, using 17 antibacterial drugs including penicillin, cefazolin, tetracycline and quinolone group. The multiple drug resistant patterns were found in most strains isolated from pig and chicken.
Rearing managements of pig farms and survey on pneumonia of slaughtered pigs
Chu Keum-Suk ; Yuk Hyun-Su ; Chon Hee-Woong ; Song Hee-Jong ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 27~36
This studies were carried out to investigate the rearing managements of pig farms and survey on pneumonia of the slaughtered pigs from 5 selected herds located in Jangsu, Jeonbuk. Isolated aerobic microorganisms from pneumonic lung were examined antibiotic susceptibility and tested serological antibody titers of the herd base. Prevalence rate of pneumonia were examined according to rearing and health management conditions of pig farms. Prevalence rate of pneumonia were detected in 78.8% and enzotic pneumonia. In 47.7%, pleuropneurnonia in 31.1%. In serological antibody titers showed the positive reaction with 54.4% in Actinobacillus pleuropneumonia serotype 5, 44.8% in Pasteurella multocida, 36.8% in A pleuropneurnonia serotype 2, 13.6% in Mycoplasmal pneumonia. Isolated aerobic microorganisms were examined antibiotic susceptibility and showed the high activity in gentamicin (58.3%), enrofloxacin (53.3%), norfloxacin (51.6%), cephalothin (41.7%) and low activity in amoxycillin (98.3%), oxytetracycline (98.3%), penicillin G (90.0 %), tetracycline (88.4%), ampicillin (88.3%). Farm managements were deficient effect of humidity in swine house but ammonia gas all appeared the 10 ppm that were recommendation density, below.
The antimicrobial susceptibility and plasmid profile of E coli isolates from wild bird
Kwak Hyun-Jeong ; Lee Woo-Won ; Kim Jam-Hwan ; Chung Kyung-Tae ; Woo Byung-Gil ; Lee Gang-Rok ; Lee Dong-Soo ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 37~46
The present study was conducted to investigate serologic characteristics, antimicrobial resistance and plasmid profile of 79 E. coli isolated from faecal sample of the wild bird in Busan province (Gangseo, Saha) during the period from November 2004 to February 2005. Many strains were positive for rafiinose (63.3%), sorbitol (96.2%), sucrose (73.4%) and few strains were positive for esculin, arginine dihydrolase, ornithine decarboxylase. Among 79 isolates, O serotypes were O55, O158, O18 and 47 strains (59.5%) were resistant to antimicrobial drugs such as tetracycline (40.5%), carbenicillin (27.8%). 15 strains showed 14 different plasmid bands composed of 1 to 6 bands. They were ranged from 1.6 to 43 kb and harbored 43 kb (11.4%), 5.3 kb (6.3%) and 11.4 kb (5.1%). Plasmid profile of 15 strains were 43 profile (2.5%), 2.4, 43 profile (2.5%) and 5.3, 11.4, 43 profile (2.5%).
Two cases of antibiotic therapy in dog infected with Brucella canis
Kim Seong-Guk ; Kim Yeong-Hwan ; Park In-Hwa ; Jang Seong-Jun ; Jo Gwang-Hyun ; Lee Yang-Soo ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 47~53
For examination of antibiotic therapeutic efficacy in canine brucellosis, this examination was carried out two female bitches infected with Brucella canis in Gyeongbuk province, and used combicillin, baytril and doxycycline in susceptible antibiotics at B canis. During 18 month after the termination of antibiotic therapy, blood sample of the two bitches were examined for B canis antibody and antigen. The antibody of one bitch was disappeared at 5 month after antibiotic therapy and the other was continued at 18 month, but two bitches were not detected antigen by blood culture and PCR. Examination of blood chemical value (AST, ALT, urea, creatinine) of two bitches was increased in AST value during antibiotic therapy.
Effects of acute LeeKwaDoo administration on liver regeneration induced by partial hepatectomy in rats
Cho Jin-Youn ; Song Ji-Yae ; Ahn Jae-Bum ; Kim Hyun-Seok ; Kim Min-Su ; Im Hyun ; Lim Jun-Sung ; Shin Dong-Seok ; Kim Hyun-Chul ; Jung Ki-Soo ; Shin Myung-Kyun ; Lee Min-Jae ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 55~63
Alcoholism and alcohol abuse are major public health concerns. This is linked to the injury of many organs, especially liver. Experiments were peformed to know the acute effects of LeeKwaDoo (LKD) induced by two-third partial hepatectomy (PH) in rats. In liver samples, regeneration parameters and histological assessment were performed. For the blood biochemical study, the blood were assayed with AST, ALT. The portal branch of liver lobes was ligated in the male Sprague-Dowley rats, two-thirds partial hepatectomies were also performed. It was estimated bodyweight and relative liver weight for the index of liver mass. For the marker of blood chemistry, we investigate the serum sample of rats and demonstrated the level of AST, ALT. Remaining tissues of liver developed as microscopic structures. Resection of the lobes in PH+LKD group resulted in a marked change of liver weight, blood chemistry and histological changes. The initiation of the proliferative response in PH group stimulated as well as reduction of the liver mass. On the other hands, the Initiation of the proliferative response in PH+LKD group delayed. Eventually, both PH group and PH+LKD group was restored relative liver weigh after 7 day. In conclusion, the acute adminstration of LKD seems to inhibit the initial response of liver regeneration through alcohol effects.
Prevalence of parasite infection of chickens in Jeonbuk province
Choi Eun-Young ; Jang Sae-Gun ; Jo Young-Suk ; Kim Ji-Young ; Im Jeong-Cheol ; Chon Hee-Woong ; Yang Hong-Ji ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 65~70
In order to detection of the intestinal parasites, fecal samples were taken from broiler (n=290), parent stock (n=168) and laying hen (n=114) in Jeonbuk province. The prevalence and identification of intestinal parasites were determined by the fecal examination using the floatation method and microscopical examination, respectively. The detection rate of parasite-eggs from 3 flocks (total=572) was 44.9%. In the breed and type of breeding, infection rate of parasite-eggs was detected 65.5% as broiler (floor breeding, 290 chickens), 20.2% as parent stock (floor breeding, 168 chickens) and 28.9% as laying hen (cage breeding, 114 chickens), in order. In the concern of mixed infection such as single and double, the rate were 40.7% and 4.2%, respectively. Six kinds of infective eggs were isolated 257 fecal sample from 3 flock. They were classified 74.7% as Eimeria spp, 18.1% as Ascaridia galli and 6.0% as Capillaria spp and 0.4% as Heteratkis gallinarum, Railleina spp or Trichostrongylus spp, single or in combination.
Preferences and animal welfare of mice for type of bedding material
Lim Jun-Sung ; Shin Dong-Seok ; Cho Jin-Youn ; Kim Hyun-Seok ; Kim Min-Su ; Im Hyun ; Ahn Jae-Bum ; Song Ji-Yae ; Kim Hyeon-Cheol ; Jung Ki-Soo ; Lee Mi-Sook ; Park Young-Jae ; Shin Myung-Kyun ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 71~77
The type of bedding material has been reported affect the environment and animal well-being. Therefore, it has an impact on the health. So, bedding material is the most Important factor in mouse environments. If it is not properly treated, experimental results are unreliable. In this study, various types of bedding material were evaluated in terms of physical characteristics and preference. It was found that bedding material consisting of large fibrous particles and wide inter-particular gap were preferred. The characteristics of bedding material were further investigated by scanning the size and shape of particles. The results show that physical characteristics such as shape, ammonia absorption, particle size effect on experimental data.
Accidental case of large colon impaction in a horse
Berzina Dace ; Zeeshan Muhamad ; Chekarova Irina ; Park Hee-Jin ; Yoon Hyun-Sang ; Lee Seung-Yeon ; Oh Myong-Ho ; Kim Bum-Seok ; Lim Chae-Woong ;
Korean Journal of Veterinary Service, volume 29, issue 1, 2006, Pages 79~82
A 5 years old gelding (Thoroughbred, Equus caballus) had shown severe abdominal pain, colic, after overeating of hay in the feed storage. following through treatment, it subsequently died. Grossly, the large colon was impacted with firm mass of food and congestion of blood vessel in the intestinal wall. There were mild peritonitis and pleuropneumonia. This case demonstrates typical large colon impaction with hard consistence ingesta due to improper management of horse.