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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 30, Issue 4 - Dec 2007
Volume 30, Issue 3 - Sep 2007
Volume 30, Issue 2 - Jun 2007
Volume 30, Issue 1 - Mar 2007
Selecting the target year
Simultaneous determination of aminoglycoside antibiotics by HPLC/MS
Roh, Young-Sun ; Baek, Gui-Jung ; Kim, Seung-Yong ; Choi, Eun-Young ; Seo, Heyng-Seok ; Hur, Boo-Hong ; Joung, Dong-Suk ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 1~12
A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics (AMGs) using Heptafluorobutyric acid (HFBA) as a ion-pairing reagent. AMGs (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry (ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.
Study on analytical method of fluoroquinolone residues in eggs by LC/MS/MS
Choi, You-Jeong ; Yun, I-Ran ; Nam, Sang-Yun ; Park, Young-Ho ; Kim, Byeong-Hun ; Son, Seong-Gi ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 13~21
An atmospheric pressure chemical ionization (APcI) LC/MS/MS method was developed for the simultaneous analysis of fluoroquinolones (norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin) residues in eggs. The spiked and blank samples were extracted from whole eggs using 50mM phosphate buffer (pH 7.4). The extract was cleaned up by passage though
MAX extraction cartridge for solid-phase extraction followed by elution with 4% formic acid in methanol. The extract of sample was separated on a Waters
reversed-phase column (
) and analyzed by APcI positive mode mass spectrometry. The mobile phase consists of aqueous 0.2% nonafluoropentanoic acid (NFPA) and methanol. Multiple reaction monitoring (MRM) using the precursor to product ion combinations of m/z
were used to quantify norfloxacin (NOR), ciprofloxacin (CIP), enrofloxacin (ENR) and danofloxacin (DAN), respectively. The limits of quantification (LOQ) were 7.8ppb for NOR, 8.5ppb for CIP, 8.9ppb for ENR, and 4.8ppb for DAN. Average recoveries of fortified sample at levels of 0.025 to 0.1 ppm were estimated 71.29% for NOR, 75.27% for CIP, 85.51% for ENR and 81.22% for DAN. These results could be applied for the confirmation and quantification in eggs.
Analysis of important quality-related components in honeys collected in Incheon metropolitan area
Lee, Sung-Mo ; Hong, Jee-Young ; Park, Eun-Jeong ; Kim, Jung-Im ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 23~32
A total of 60 samples (38 domestic honeys and 22 foreign honeys) were collected from October in 2003 to May in 2004 in Incheon metropolitan area, and contents of important quality-related components in honeys were analyzed using methods of Korea Food Code. Most of the honeys were satisfied with Korean limit (21%) in moisture content even though 4 samples brought by Korean travellers and 1 sample in domestic honey (wild flower) were higher than the limit. The lowest mean level of moisture in the honeys was imported foreign honeys (16.95%). All of the samples were below of 0.6% in the analysis of ash contents. The highest content was imported foreign honey (0.19%) while the lowest content was in domestic acacia honey (0.05%). Acidity of domestic honeys (9.49-9.94meq/kg) was approximately half of that in foreign honeys. All samples were satisfied with Korean limit (40.0meq/kg). In the analysis of fructose and glucose, only three samples of foreign honeys were lower than Korean limit (sum of both ; 65%). The content of sucrose in all samples was less than 7%. Mean of Hydroxymethylfurfural (HMF) contents were 12.83-24.3mg/kg in domestic honey, and 31.34-45.58mg/kg in foreign honey. However, three samples brought by Korean travellers were not satisfied with Korean limit (
). In conclusion, quality of domestic honey was better than that of foreign honey. Also, it may be needed that the honeys purchased by Korean travellers in foreign countries should be continuously monitored.
Outbreak of Fowl Cholera in native chickens
Lee, Jong-Jin ; Kim, Hwan-Hee ; Byun, Chul-Sub ; Lee, Eun-Jeong ; Yuk, Min-Jeong ; Park, Jae-Myoung ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 33~42
Fowl cholera(FC), which has been classified as reportable animal disease in Korea, occurred in the backyard farm located in Chungju, Chungbuk province. The farm was not ordinary domestic poultry farm but backyard farm with main cultivation of fruits. The farmer reported unusual mortality of chickens which were grazed in his own fruit farm without any poultry house. There was a lots of opportunities to contact with wild animals including wild birds freely. Clinical signs observed in the chickens were torticolis, mucous excretion from mouth and greenish diarrhea. On the necropsy we found multifocal necrosis on the surface of liver, hemorrhages in the epicardium and ovary and mucopurulent exudates in the joint. The causative agents was isolated from the liver of chickens collected from the farms and identified as Pasteurella multocida using biochemical tests of bacteria. Also, the isolated bacteria were inoculated into specific pathogen free chickens to evaluate the pathogenicity. High mortality and similar pathological lesions compared to those of chickens died in the farm were observed. We report the first case of fowl cholera of the chickens in Korea and further studies are needed to evaluate the serotype, genotype and pathogenicity of isolated Pasteurella multocida.
Examination of seroprevalence and detection of Avian pneumovirus from layer hens in Gyeongbuk province
Kim, Jung-Eun ; Hwang, Ji-Young ; Bae, Dong-Rok ; Sung, Myoung-Suk ; Kim, Soon-Tae ; Kim, Sang-Yun ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 43~49
Avian pneumovirus(APV), also known as avian rhinotracheitis(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis(TRT). The aim of this study was to establish the presence or absence of antibodies to APV by an enzyme-linked immunosorbent assay(ELISA) and confirm APV by a reverse transcriptase-polymerase chain reaction(RT-PCR). The tested serum and feces were collected from laying hens in Gyeongbuk province. The positive farms with antibody against APV by ELISA were 90(96.7%) of 93 and positive serum samples were 433(93.1%) of 465 different sera. By regional group, sera from Uiseong, Cheongsong and Bonghwa were noted as 100% positive and positive rates of samples from Yeongju, Andong and Yeongyang were 93.3%, 85.7% and 50%, respectively. However, APV was not detected in feces samples by RT-PCR.
Effect of porcine reproductive and respiratory syndrome virus on pigs with pleuropneumonia - Studies on serology and proportion of porcine subpopulation of peripheral blood -
Jeong, Hyun-Kyu ; Park, Yong-Ho ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 51~66
The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A pleuropneumonia. Twenty three 7-week-old commercial pigs were infected intratracheally with PRRS virus and/or A pleuropneumoniae serotype 5. Serum antibody titers were examined by an enzyme-linked immunosorbent assay(ELISA) and proportion of porcine leukocyte subpopulations in peripheral blood was examined by flow cytometry. In this experiment, antibodies against PRRS virus and A pleuropneumoniae were detected at 2 weeks and 1 week postinfection and the number of antibody positive pigs were gradually increased. And in proportion to leukocyte subpopulations in peripheral blood of pigs infected with A pleuropneumoniae compared with pigs administrated with saline, the proportion of PoCD4 and N cells were increased(P<0.1). Furthermore, in proportion to leukocyte subpopulations in peripheral blood of pigs infected with PRRS virus followed by A pleuropneumoniae compared with pigs administrated with saline, the proportion of MHC class II, PoCD4 and B cells were significantly increased(P<0.1). The results indicated that dual infection with PRRS virus and A pleuropneumoniae induced the stronger immune responses associated with macrophages and Th cells in pigs than single infection with PRRS virus or A pleuropneumoniae.
Investigation of antibodies to bovine leukosis virus from Korean indigenous cattle in Jeongeup area
Shon, Ku-Rye ; Lee, Jeoung-Won ; Lee, Hee-Mum ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 67~75
Bovine leukosis is one of the important diseases in Korea because of economic losses, and this study was conducted to provide basic information for the control of the disease. A total of 2,104 sera were obtained from 491 farms and examined by commercial kit. In the seroprevalence of farms and heads, 71 farms out of 491 were seropositive (14.5%) and 119 heads were positive (5.7%) out of 2,104. In the areal distribution of seropositiveness, Soseong-myeon showed the highest rate (39.5%, 15/38 farms)and Yeongwon-myeon was the lowest (2.6%, 1/38 farms). By age, positive rate in the below of 1 year old cattle was much less than that in the over of 1 year old, namely, the former was 1.9% (3/161 farms) and the latter 20.6% (68/330 farms). According to the farm size, there was no significant difference among the size. However, the highest rate was shown in the farms having 50-99 heads, 23.2% (13/56 farms).
Studies on Campylobacter jejuni and Campylobacter coli contamination on broiler carcasses in slaughterhouse
Na, Ho-Myung ; Koh, Ba-Ra-Da ; Park, Seong-Do ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 77~84
The present study was carried out to investigate the incidence of Campylobacter spp. from the chicken carcasses in slaughterhouse. A total of 9 strains were primarily isolated from enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Nine of Gram-negative, catalase-positive and oxidase-positive strains were further isolated by the determination of biochemical characteristics and finally identified as Campylobacter jejuni with HIP 400F and HIP l134R primers. Therefore, this PCR method proved to be useful as a routine diagnostic test for the Campylobacter detection and confirmation of C. jejuni and C. coli in naturally contaminated poultry samples.
Investigation of post - weaning atrophic pig diseases in swine breeding complex in Jeonbuk - Iksan
Chu, Keum-Suk ; Jo, Young-Suk ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 85~93
The purpose of this study was to investigate the infection situation of several diseases (post-weaning atrophic pigs) such as porcine reproductive and respiratory syndrome (PRRS) in swine breeding complex in Jeonbuk-Iksan. From February to October in 2006, a total of 28 swine samples (6-10 week old) were collected from 6 farms and examined by polymerase chain reaction(PCR) and clinical signs. In the rate of single infection, pneumonia was top (32.1%), followed by salmonellosis (14.2%)and Glasser's disease (10.7%) and double infection pneumonia/Glasser's disease (17.8%) was detected. PCR was detected of PCV 2 from 28 (100.0%) and PPV 6 (21.4%), PRRS PORF6 10 (35.7%) and POR7 11 (39.2%), but HC and AD was not detected. The results suggest that PCV 2 is complex infection PRRS, PPV and bacterial disease.
Seroprevalence of infection with Neospora caninum, Mycobacterium paratuberculosis, bovine leukosis and Brucella abortus of dairy cattle in Jeonbuk-Iksan area
Chu, Keum-Suk ; Hyong, Sang-Gi ; Im, Jeong-Cheol ; Seo, Lee-Weon ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 95~102
The purpose of this study was to investigate the seroprevalence of infection with the production-limiting diseases in dairy cattle in Jeonbuk-Iksan area. The blood samples were collected from 260 dairy cows in 52 herds, and examined. The antibody rates against N caninum, M paratuberculosis, and bovine leukemia virus were 34.6%, 13.5% and 89.6%, respectively. All samples for bovine brucellosis were negative.
The trend of antimicrobial resistance of Escherichia coli isolated from healthy volunteers of community and chicken butchers in Incheon
Hwang, Kyoung-Wha ; Kim, Hye-Young ; Lee, Mi-Yeon ; Koh, Yeon-Ja ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 103~113
We monitored antibiotic resistance of Escherichia coli isolates from healthy volunteers of community and chicken butchers from February to July in 2006. From disc diffusion test on 473 E coli isolates from healthy volunteers of the community, the resistance rates of tetracycline, ampicillin, and ticarcillin were 44.8%, 40.8%, and 37.4%, respectively. In the disc diffusion test on 18 E coli isolates from chicken butchers, the resistance rates of tetracycline, nalidixic, streptomycin, and ampicillin were 94.4%, 61.1%, 55.6%, and 50.0%, respectively.
Therapeutic effects of honeybee (Apis Mellifera L.) venom injection on bovine mastitis
Han, Sang-Mi ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Kweon, Hae-Yong ; Woo, Soon-Ok ; Oh, Baeg-Young ; Lee, Yun-Geun ; Kim, Bong-Soon ; Baek, Ha-Ju ; Kim, Soon-Tae ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 115~123
The therapeutic effect of honeybee venom collected using be venom collector on bovine mastitis was investigated. Mastitic cows from four farms were selected in the Yang-pyeong areas. Chronic mastitic cows were injected with the various concentrations of honey-bee venom per day. There was a significant difference in the reduction rates of somatic cell counts (SCC) according to treatment concentration and method of bee venom. The milk SCC were significantly decreased in all concentrations of bee venom 3 days after treatment. The reduction rates of SCC in treatment of 3, 6, 12 and 24mg honeybee venom were 20, 43, 63.3 and 65.8% respectively. Honeybee venom treatment consisted of two methods, a syringeful and a Bovivet Spenstift. The treatment with Bovivet Spenstift was more effective in the reduction rates of SCC compared with the syringeful. Thirty two out of 53 quarters were cured by Bovivet Spenstift with 12mg bee venom per day for 14 days. The venom cure rates of bovine mastitis by Escherichia coli, Stapylococcus aureus, Gram positive bacteria and Gram negative bacteria were 33.3, 75, 75 and 43.8% respectively. These results suggested that bee venom treatment (by Bovivet Spenstift with 12mg) might be effective for treatment of bovine mastitis.
Identification of the causes of anemia and differential diagnosis of immune-mediated hemolytic anemia in anemic patient dogs
Lee, Youn-Kyung ; Lee, Chai-Yong ; Nam, Hyang-Mi ; Kang, Mun-Il ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 125~132
Anemia is a common problem in sick dogs, and immune-mediated hemolytic anemia (IMHA) is one of the most common causes of anemia in dogs. Since death can occur rapidly in dogs with IMHA even with appropriate treatment, it is important to differentiate IMHA from other causes of anemia in its first stages. To diagnose underlying diseases in anemic dogs and differentiate IMHA cases from others, 29 patient dogs suffering from severe anemia that had been referred to Veterinary Medical Teaching Hospital at a National University from June 2004 to April 2005 were examined. The most common cause of anemia in the patient dogs was found to be liver disease accounting for 31.0% (9/29) of all, and the second most common was IMHA with 13.7% (4/29). Four dogs confirmed as IMHA cases all reacted positive to direct anti-globulin test and showed spherocytes and polychromatic erythrocytes in the blood smear. Most of the IMHA cases (3/4) were female aged 2 to 7 years and were in a severe state of anemia with less than 20% of PCV.
A Study of the correlation between breeding density and the azimuth of cattle sheds affected calf diarrhea disease
Lee, Yun-Lyul ; Lee, Hak-Rim ; Ahn, Jae-Bum ; Song, Ji-Ye ; Jang, Jae-Jin ; Lee, Min-Jae ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 133~144
This study was conducted to determine the effective environmental control measures in preventing calf diarrheal disease in farms. It was found that the high numbers of calves in a cow house was directly related with the high incidence rate of diarrheal diseases. When the number of calves was increased in a cow house, it was difficult to reduce the diarrheal diseases of calves. It was revealed that the incidence rates of calf diarrheal disease were dramatically reduced when the delivery house which faced south. The periodical changes of bedding materials seemed beneficial good to control the possible resident infectious agents, including pathogenic viruses and bacteria. The control of the delivery time was important for reduction of calf diarrhea. It was revealed that September and October were the best time as the delivery period. In order to use old cow house as a delivery house, it should be sterilized by the disinfectant and the flame. When the diarrheal feces were removed as soon as possible, the transmission of diarrheal diseases among calves In the same house was reduced. When the diarrheal feces were not disposed, the incidence rate of diarrhea reached up to 50%. When the morbid calves were not quarantined, the incidence rate of diarrhea was 100%. In contrast, when the diseased calves were quarantined from other calves, the incidence rate of diarrhea was reduced, remarkably. The mean recovery time from the diarrhea of the treated calves in the southern cow house was much faster than that in the north cow house. The treatment of calves in a clean and well-ventilated cow house with dry bedding was more effective than that in a cold, wet, and dark house. It could be postulated that the most important environmental factor in the reduction of diarrheal diseases of calves is the blocking of the entry of the infectious agents. The complete sanitation and control of the delivery house in the using farm is more important than building a new cow house, for prevention of calf diarrheal diseases.
Evaluation of the criteria to distinguish heifer from cow of Holstein cattle in abattoir
Kim, Kyoung-Ho ; Lee, Jung-Goo ; Ra, Do-Kyung ; Kim, Cheol-Wan ; Byun, Jae-Won ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 145~154
In Korea, it is the one of controversial problems to distinguish heifer from cow in slaughtered Holstein cattle. This study was conducted to evaluate the several criterions which could be used to discriminate heifer from cow. Some criterions have shown significant differences between heifer and cow in ante and post-mortem inspections(p<0.01). Firstly, the numbers of milk teeth of heifer and cow were
respectively. The teat diameter(D) and length(L) of cow were
respectively. However, those of heifer were significantly smaller (D:
) than those of cow. The size of udder was
in Holstein cow and
in heifer. Secondly, the uterus size of cow was significantly bigger than that of heifer and caruncle in mucosa of uterus could be easy to be confirmed by necropsy inspection. It was also obvious that the folding and length of uterus body were significantly remarkable in cow. Lastly, the pelvic cavity was
in heifer and
in cow. The ossifying maturation of heifer was
in cow. As the results, this study can be helpful for meat inspectors to discriminate the non-delivery heifer from delivery cow in Holstein cattle.
Investigation of canine dirofilariasis and brucellosis in free roaming dogs from public animal shelters in Gwangju area
Koh, Ba-Ra-Da ; Na, Ho-Myung ; Jang, Mi-Sun ; Kim, Ji-Yeon ; Park, Seong-Do ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 155~164
This study was conducted to investigate the prevalence of canine heartworm infections, canine brucellosis and hematologic values from 153 free roaming dogs in the area of Gwangju city from March to November 2006. Nineteen (12.4%) of 153 samples tested with modified Knott's technique showed positive reaction for microfilariae. Polymerase chain reaction using specific primers for D immitis amplified the expected product from all samples of 19 microfilaremic canine blood samples as determined by the modified Knott's test for microfilariae. The seasonal infection rates of microfilariae were higher in the spring season (10/19, 52.6%) than in the other seasons. The major hematological findings in microfilaremic dogs were mild leukocytosis and mild monocytosis. A total of 100 dogs randomly selected from 153 free roaming dogs were negative for canine brucellosis by serological test using immunochromatographic antibody test kit.
Contamination of Toxocara canis in soil of playground in a detached dwelling area of Daejeon city
Chung, Nyun-Ki ; Lee, Seok-Ju ; Han, So-Young ; Park, Jong-Min ; Ha, Sook-Hee ; Jang, Seung-Ik ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 165~173
In order to survey the prevalence of Toxocara cams in soil of playground in a detached dwelling area, 150 samples were taken from playground 50 unit. The survey was carried out during 3 months from July to September in 2006. The Egg of Toxocara canis were detected in 6 samples (4.0%) from 150 cases of playground. The prevalence of T canis by month was 6.0% in July, 4.0% in August, 2.0% in September, respectively. This study results suggested that the prevalence of T canis were decreased in 2006 compared to 18.5% (30 positive/162 samples) in 2000.
Prevalence of internal parasites in housed cattle farms in Gangwon-do
Cheong, Ki-Soo ; Kim, Ji-Tae ; Lee, Min-Jae ; Jung, Bae-Dong ; Ahn, Dong-Choon ; Kim, Jong-Taek ; Kim, Hyeon-Cheol ;
Korean Journal of Veterinary Service, volume 30, issue 1, 2007, Pages 175~181
Parasites cause great economic loss in livestock in Korea, and can be categorized as either or indirect losses. In order to monitor the parasites from October 2005 to January 2006, 401 samples were randomly collected from cattle farms and examined the prevalence of gastro-intestinal (GI) parasites by using the sucrose flotation method and formalin-ether centrifugation method. The overall infection rate of GI parasites was about 43.4%. Of these parasites, nematodes were identified as Capillaria bovis (5.5%), Strongyloides papillosus (2.5%), Trichuris discolor (1.2%). Cestoda was observed only one species, Moniezia benedeni (2.2%). The rate of Eimeria spp was 39.2%. A total of five species of Eimeria was identified; among the species identified as above E bovis and E zuernii appeared with relatively higher infection rates. The combined infection of parasites was 35.2% in single species, 29.0% in double and 2.0% in triple infections. No cases of clinical symptoms were observed in this survey. The EPG levels of each species of parasites were very low in all infected cases.