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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 30, Issue 4 - Dec 2007
Volume 30, Issue 3 - Sep 2007
Volume 30, Issue 2 - Jun 2007
Volume 30, Issue 1 - Mar 2007
Selecting the target year
Development of oligonucleotide microarray system for differential diagnosis of enteric viruses in diarrheic fecal samples in pigs
Park, Nam-Yong ; Kim, Yong-Hwan ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 489~496
An oligonucleotide microarray system was developed for the simultaneous detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, porcine enteric calicivirus, porcine group A and C rotavirus. RNAs of the reference viruses and porcine diarrhea samples were extracted and amplified using one-step multiplex RT-PCR in the presence of cyanine 5-dCTP and hybridized on the microarray chip that spotted the virus-specific oligonucleotides. This system were approximately 10-to 100-fold higher in sensitivity than conventional RT-PCR, and the assay time was less than 3 hours. The relative sensitivity and specificity were 92% and 72.2%, respectively, based on 102 porcine diarrhea samples using RT-PCR as gold standard. These results suggested that the oligonucleotide microarray system in this study be probably more reliable and reproducible means for detecting porcine enteric viruses and that it could be of substantial use in routine diagnostic laboratories.
Immune response and efficacy of pigeon pox virus vaccine and fowl pox virus vaccine in chickens
Shil, Niraj Kanti ; Rahman, Md Siddique ; Hossain, M.T. ; Islam, M.T. ; Rahman, M.M. ; Islam, K.B.M. Saiful ; Cha, Se-Yeoun ; Jang, Hyung-Kwan ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 497~503
The humoral immune response of chicken vaccinated with fowl and pigeon pox virus vaccines was determined with the protective potentiality of the two vaccines in field condition of Bangladesh. Different aged Fayoumi chicks were subjected for the study. To assess the relationship with better immune response among experimental groups, the average percentage of 'take reaction' was examined and recorded to 97.77% in group A, 93.33% in group B and 100.0% in group C. The level of immune status induced by different vaccinated group was measured by passive hemagglutination (PHA) microplate test method. The mean PHA titer levels after primary vaccination were
in group A,
in group B, and
in group C. Following booster vaccination, the mean PHA titer levels in prior of challenge were increased to
in groups A and C, and
in group B. The recorded PHA titer levels of each group at two weeks after challenge were significantly increased to
, respectively. The PHA titer levels after vaccination and challenge were significantly increased compared to pre-vaccination titer levels (P<0.01). Although the PHA titer levels among three groups administrated different vaccine combinations in prior of challenge were significantly varied (P<0.01), it was observed that all of the vaccinated chicks were highly protected against challenge infection.
Detection of beta-lactam antibiotic resistant genes in Escherichia coli from porcine fecal samples using DNA chip
Park, Nam-Yong ; Na, Sung-Ho ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 505~510
This study was conducted to detect
-lactam antibiotic-resistant genes in the 400 E coli isolates from porcine fecal samples in Korea by a DNA chip. The DNA chip contains the specific probe DNAs of the
-lactam antibiotic-resistant genes that had been labeled with a mixture of primer set designed to amplify specific genes (PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY and AmpC) using a multiplex polymerase chain reaction (PCR). Of 400 isolates 339 contained at least one
-lactamases gene. Resistance to
-lactamases was mediated mainly by AmpC (n = 339, 100%), and followed by TEM (n = 200, 59.0%), CMY (n = 101, 29.8%), PSE (n = 30, 8.9%) and both OXA and SHV genes (n = 20, 5.9%), while the FOX, MEN and OXY genes were not detected. The other sixty-one did not contain any
-lactamase genes even though they were resistant to antimicrobial drugs. In conclusion, the DNA chip system can be used as a rapid and reliable method for detecting of
-lactamases genes, which will help veterinarians select the antibiotics for monitoring and treating of animal diseases.
Seroprevalence of brucellosis in small ruminants in selected area of Bangladash
Uddin, Mohammad Jasim ; Rahman, Md Siddiqur ; Akter, Sayeda Hasina ; Hossain, Mohammad Arif ; Islam, Md Taohidul ; Islam, Md Ariful ; Park, Jin-Ho ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 511~525
A seroprevalence study of small ruminant brucellosis was conducted in sheep and goat rearing selected areas of Mymensingh district and Dhaka district, Bangladesh, from March, 2005 to May, 2006. Sera from 62 sheep and 300 goats were tested by rose bengal plate test (RBPT), plate agglutination test (PAT), tube agglutination test (TAT) and mercaptoethanol test (MET). Out of the 62 sera tested 3.25% (n = 2) were positive to RBT, PAT and TAT and 4.84% (n = 3) were positive MET. In case of 300 goats, 1.67% (n = 5) were positive to RBT and PAT, 2% (n = 6) were positive to TAT and 2.33% (n = 7) were positive to MET. This investigation is the first of its type to be performed in small ruminants in Bangladesh. Higher prevalence rate (8.0 %) was found in BAU nutrition farm in case of sheep and 10 % in Bangladesh Agricultural University (BAU) Veterinary Clinic in case of goat while lower prevalence (0.0 %) was recorded in Pharmacology project and BAU adjacent villages in case of sheep and (0.0 %) in Dhamrai upazila in case of goats respectively. Brucella antibodies were more prevalent in sheep (8.84 %) than in goat (2.33 %).
Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo
Shin, Jong-Bong ; Park, Nam-Yong ; Kim, Yong-Hwan ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 527~532
In present study, we described the reliability of the dual priming oligonucleotide (DPO) multiplex polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis complex (MTC) and non-Mycobacterium tuberculosis (NMT) in blood samples of the Korea native cattle, Hanwoo. Among 340 samples 22 (6.5%) were positive in using DPO multiplex PCR, 21 (6.2%) were positive in PCR. The relative agreement between 2 tests was 99.7%, and the agreement quotient (kappa), was 0.95 (excellent). In these results, we demonstrated the successful application of DPO multiplex PCR for the diagnosis of bovine tuberculosis in Hanwoo. Multiplex PCR, using DPO primers, can be useful for the simple diagnosis of bovine tuberculosis in bovine blood samples.
Histopathologic and electron microscopic findings of canine malignant melanoma from the lower limb and digit
Woo, Gye-Hyeong ; Kim, Ha-Young ; Park, Jung-Won ; Bak, Eun-Jung ; Kim, Jin-Young ; Choi, Cheong-Up ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 533~538
A 16-year-old female mixed dog was submitted for examination at the pathology division of national veterinary research and quarantine service (NVRQS). Grossly, white or grayish spherical, multinodular, firm to friable masses were present in the tibiofibula to the pharenge area of the right limb, and dysphagia, breathing difficulties and tachypnea were shown. Various-sized white or grayish black masses were scattered in lungs and diaphragm and one mass was observed in the trachea and in the jejunum, respectively. Histopathologically, the neoplastic cells were composed of polygonal or spindle shaped cells with various sized round to oval nuclei and abundant cytoplasm. These cells formed lobules or nests separated by fine connective tissue and contained little amount of melanin pigments. Melanin pigments were stained dark gray or black with Fontana-Masson method. Melanosomes were also ultrastructurally demonstrable by electron microscopy. Based on above results, the present case was diagnosed as canine malignant melanoma originating from the lower limb and digit.
Canine hemangiopericytoma in a Golden Retriever: A case report
Kim, Ha-Young ; Woo, Gye-Hyeong ; Hwang, Bum-Tae ; Park, Jung-Won ; Choi, Cheong-Up ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 539~544
A 7-year-old intact female Golden Retriever was presented for examination. The dog had large irregular subcutaneous masses in the abdomen which were ruptured or encapsulated. Those were removed surgically. Histopathologically, the masses consisted of spindle cells that often formed distinct whorls around a central capillary. Immuno-histochemical analysis revealed that the neoplastic cells were strong diffuse cyto-plasmic immunolabelling for vimentin and focal immunoreactivity for smooth muscle actin, whereas not immunoreactive for cytokeratin, desmin, von Willebrand factor, glial fibrillary acidic protein, or S-100. The neoplastic cells ultrastructurally had processes attached by desmosome-like structures, swollen mitochondria and dilated rough endoplasmic reticulum. Based on the above results, this case was diagnosed as a canine hemangiopericytoma in the abdominal subcutis of a Golden Retriever.
Monocrotophos poisoning in wild mallards (Anas platyrhynchos)
Shim, Hang-Sub ; Kim, Hae-Sung ; Woo, Jong-Tae ; Kim, In-Seop ; Jung, Hae-Sun ; Song, Eun-Ah ; Bark, Jun-Jo ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 545~550
The toxicity of organophosphate arises from disruption of the nervous system due to the inhibition of cholinesterase enzymes, leading to death. Six dead mallards were found at Ansung where is one of the most popular wintering sites for migratory birds in Korea, and requested for diagnosis to Gyeonggi Veterinary Service on January of 2007. Some examinations including polymerase chain reaction (PCR) could not find any evidence of specific disease condition. However, the contents of gastrointestinal tracts of the birds contained residues of monocrotophos ranged from 31.3ppm to 294.3ppm by gas chromatography and mass spectrometry. It can be supposed that monocroptophos was responsible for the death of mallards by this results.
Bovine leukosis in a slaughtered cow
Lee, Sang-Myung ; Choi, Hae-Yeun ; Kwak, Hak-Koo ; Jung, Chan-Woo ; Han, Seong-Tae ; Park, Sun-Jun ; Yoon, Ho-Gyu ; Jang, Rae-Hun ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 551~555
A slaughtered 3 years old cow was diagnosed as bovine leukosis by postmortem inspection, agar gel immunodifusion (AGID) test, and polymerase chain reaction (PCR). It showed no special clinical signs except emaciation. Grossly, we observed enlargement of mediastinal and pelvic lymph nodes, and tubercle -like mass on pleura and lung surface, and masses on right atrium and ventricle.
Application of the melanocortin 1 receptor (MC1R) gene for discrimination of Hanwoo from Holstein beef using real-time polymerase chain reaction (PCR)
Ra, Do-Kyung ; Lee, Sung-Mo ; Park, Eun-Jeong ; Lee, Jung-Goo ;
Korean Journal of Veterinary Service, volume 30, issue 4, 2007, Pages 557~562
This study was carried out to discriminate Hanwoo from the milking and hybrid cattle by detection of MC1R gene related to bovine hair color. One hundred sixty six samples were collected from the abattoir (n = 106) and local market (n = 60). The beef from abattoir were originated from Hanwoo (n=27), Holstein (n=29), Hybrid (n=45) and imported cattle (n=5), respectively. The beef from market consisted of Hanwoo (n=36), Holstein (n=7) and imported ones (n=17). Commercialized screening kit (Kogenebiotec, Korea) was used for MC1R gene analysis. As a result, Hanwoo was discriminated from Holstein. However, 9 of 45 hybrid and 11 of 22 imported beef samples were indistinguishable from Hanwoo. It could be explained by second generation of crossing of Hanwoo with Holstein or the cattle with silver or yellow hair. This results suggest that additional tests as well as MC1R gene detection be needed to confirm Hanwoo beef among cattle beef.