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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 31, Issue 4 - Nov 2008
Volume 31, Issue 3 - Sep 2008
Volume 31, Issue 2 - Jun 2008
Volume 31, Issue 1 - Mar 2008
Selecting the target year
Serotypes and genotypes of Salmonella isolates from slaughtered pigs
Choi, Won-Zong ; Jung, Ji-Hun ; Won, Ho-Keun ; Kang, Zheng-Wu ; Hahn, Tae-Wook ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 1~16
Salmonella infections cause the disease in pigs but also some zoonotic Salmonella serotypes can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detection of invA gene using Salmonella specific polymerase chain reaction (PCR), the epidemiological characteristics related to Salmonella strains cultured from pig samples in Gangwon areas using serotyping, random amplified polymorphic DNA (RAPD) and pulsed field gel electrophoresis (PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine (TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioMerieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23 (52.3%) were S Eingedi, 10 (22.7%) S Schwarzengrund, 9 (20.5%) S Typhimurium, and 2 (4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distinguishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.
Study on antimicrobial resistance of Escherichia coli isolated from domestic beef on sale
Kim, Hong-Tae ; Lee, Woo-Won ; Jung, Kyung-Tae ; Lee, Seung-Mee ; Son, Eun-Jung ; Lee, Gang-Rok ; Kim, Geum-Hyang ; Lee, Dong-Soo ; Lee, Keun-Woo ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 17~29
In this study, antimicrobial resistance of E coli isolated from domestic beef on sale in Busan and Gyeongnam province was investigated from March to October 2007. A total of 600 beef samples were collected for the monitoring of antimicrobial resistance, and 92 (15.3%) strains of E coli were isolated. Antimicrobial resistance test was carried out by agar disc diffusion method with 17 antimicrobials. In general, E coli isolates showed the highest antimicrobial resistance to doxycycline (73.9%), followed by tetracycline (70.7%) andcefazolin (63.0%). Then they showed higher resistance to several antimicrobials like norfloxacin (48.9%). However, They had low antimicrobial resistance to amikacin (4.3%), colistin (1.1%). Of 92 isolates, 82 (89.1%) were resistant to more than 2 antimicrobials. Among 17 antimicrobials examined, tetracyclines were the most resistant, followed by cephalosporins, quinolone. The resistance was seemed to be correlated to amounts of antimicrobial use. In the result of this study, we suggest that there be need to regulate the abuse of antimicrobial on food-producing animals in Korea because the concern on antimicrobial resistant is gradually increased worldwide.
Study on the seroprevalence of Salmonella Pullorum in boiler farms in Daejeon area
Han, So-Young ; Chung, Nyun-Ki ; Kim, Jong-Ho ; Park, Jong-Min ; Han, Su-Jin ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 31~35
In order to investigate the seroprevalence of Salmonella Pullorum in boilers reared in Daejeon area, 509 samples were collected from 10 boiler farms randomly selected from 38. The survey was carried out during 6 months from April to October in 2007. Out of 509 chickens, 35 (6.9%) were seropositive and average titer was
. The seroprevalence by district was 5.5% (5/90) in Dong-gu, 4.1 % (4/96) in Seo-gu, 7.9% (24/303) in Yuseong-gu, 10.0% (2/20) in Daedeok-gu.
Riemerella anatipestifer infection in domestic ducks
Lee, Jong-Jin ; Kim, Hwan-Hee ; Byun, Chul-Sub ; Park, Jae-Myoung ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 37~42
Riemerellosis, contagious disease of domestic ducks in Korea, occurred in December of 2007 in a farm located in Eumsung, Chungbuk province. The affected ducks were 22 days old and the owner reported that almost one hundred of ducks had died everyday for a few days after infection. Clinical signs were listlessness, ocular and nasal discharge, greenish diarrhea, ataxia, tremor of head and legs, and coma. On necropsy we found fibrinous exudate, which involved serosal surfaces in general, but most evidences were in the pericardial cavity and over the surface of the liver. The causative agent was isolated from the liver of the affected ducks and identified as Riemerella anatipestifer using biochemical tests. Also, the isolate was susceptible to ampicillin, cephalothin, sulfamethazole/trimethoprim, Florfenicol among the 23 species antibiotics (AST Discs, OXOID) used in our laboratory. Further studies should be needed for the more effective control and better epidemiological information such as pathogenicity, serotype, genotype and treatment.
Recently epidemiological survey of the viral diseases of broiler chickens in Jeonbuk province from 2005 to 2007
Park, Jong-Beom ; Cha, Se-Yeoun ; Park, Young-Myoung ; Zhao, Dan-Dan ; Song, Hee-Jong ; Jang, Hyung-Kwan ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 43~55
Recently, the major viral diseases, Newcastle disease (ND), infectious bronchitis (IB), low pathogenic avian influenza (LPAI), avian pneumovirus infection (APV), Marek's disease (MD) and infectious bursal disease (IBD), have led to huge economic losses in chicken industry of Korea. To evaluate prevalence of the major viral disease infections in broiler breeder and broiler farms, epidemiological survey has been conducted in Jeonbuk province from 2005 to 2007 by serological ELISA test for APV, PCR for MD, and RT-PCR for ND, IB, LPAI and IBD, respectively. A total of 424 cases was submitted to our laboratory for diagnosis of the major viral disease from broiler breeder and broiler farms in the above period. The diagnosed results were analysed for the detection rate of infections on basis of years, seasons and ages, respectively. This study was showed that the detection rates of ND and APV were considerably high for every years regardless of seasons and ages in both broiler breeder and commercial broiler. In comparison with detection rates of ND and APV, IB and LPAI were lower but detected around 10% for every years. Especially, detection rate of IB was significantly high in commercial broiler than in broiler breeder. Therefore, to minimize economic losses for broiler breeder and broiler farms, it will need for effective countermeasures to decrease detection rate of the viral respiratory diseases. Although the detection rates of MD and IBD were gradually decreased from 2005 to 2007 in both broiler breeder and commercial broiler, it will continually make an effort about disease control for increasing productivity in chicken industry.
Effects of the modified live vaccines against Bordetella bronchiseptica and canine parainfluenza virus
Park, Young-Il ; Roh, In-Soon ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 57~70
The purpose of this study was to investigate the protective effects of the modified live vaccines against canine Bordetella bronchiseptica (Bb) and canine parainfluenza virus (CPIV) in puppies by nasal administration. A total of 24 puppies were classified as 3 groups consisting of 8, and each one was divided into two subgroups; vaccinated (n=4) and unvaccinated (n=4). Group I, group II and group III were challenged with Bb, CPIV, and Bb+CPIV, respectively. In group I vaccinated puppies (n=4) were experimentally challenged with Bb 2 weeks after vaccination and unvaccinated puppies (n=4) were experimentally challenged with Bb alone. The same methods of the above were applied for group II and group III. We carried out several studies including serum tests, isolation, and histopathological examination. The vaccinated puppies showed higher antibody titers than unvaccinated puppies and the titer sustained during the experimental period. In the isolation test, recovery period was shorter in the vaccinated subgroup than in the other. In clinical signs, the unvaccinated puppies showed the typical signs of tracheobronchitis (coughing, nasal and occular discharge), but another subgroup showed delayed incidence and mild clinical signs. The typical gross lesions and histopathological findings were observed in the unvaccinated puppies. In immunohistochemical findings, the vaccinated puppies showed little intensive in reaction for CPIV antigen than the other. It could be concluded that intranasal vaccination of modified live Bb and CPIV vaccine to puppies is effective to prevent canine infectious tracheobronchitis.
Detection of porcine circovirus 2, porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae from swine lungs with lesions by PCR
Chu, Keum-Suk ; Kang, Mi-Seon ; Jo, Young-Suk ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 71~77
Today swine respiratory disease is one of the most important diseases because of its economic losses and severe infection nationwide, and swine society as well as veterinary service are trying to prevent the diseases in Korea. This study would like to obtain some information useful for the control of the diseases. A total of 174 lung specimens with lesion consisted of 3 sorts; 60 were collected from nursey pigs requested for diagnostic service from March of 2006 to October of 2007, 58 finishing pigs and 56 sows were selected from slaughterhouse from September to November 2007. In the detection test of pathogens by PCR, porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae were positive in 95.4%, 31.6%, and 20.1%, respectively. Double infection rate with PCV2 and PRRS was 30.4%, PCV2 and M hyopneumoniae was 19.5%, triple infection with PCV2, PRRS and M hyopneumoniae was 5.7%, respectively.
Epidemiological study for Neospora caninum in HanWoo and Holstein-beef cattle in Namwon areas
Kwon, Mee-Soon ; Jeong, Jae-Myong ; Lee, Ji-Yoog ; Bae, Jong-Jun ; Yoon, Yeo-Baik ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 79~86
In this study two stage investigation was used for seroprevalence of Neospora caninum in cattle between January, 2005 and November, 2007; first stage was to examine positiveness of the farms selected randomly, second was to test all individual cow in positive farms. A total of 850 sera were collected from 170 farms for the first stage, and positive rate of farm and head were 23.5% (40/170farms) and 7.5% (64/850heads), respectively. Seroprevalence of Holstein cattle was much higher than that of Hanwoo. In second stage positive rate of cow was 18.9% (246/1,303head), but seroprevalence of farm was very variable (1 - 90.5%). It was supposed that 3 farms with high positive rate had some risk factors to be infected; raising dogs in the same farm, location of hill-side where is easy to contact with wild animals.
Investigation of swine toxoplasmosis by Latex agglutination and polymerase chain reaction(PCR)
Shim, Hang-Sub ; Choi, Gyeong-Muk ; Jeon, Oh-Sook ; Lee, Su-Jin ; Woo, Jong-Tae ; Ro, Ki-Woan ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 87~91
Between March and October 2007, a total of 516 blood samples from pigs in the Gyeonggi province were examined for seroprevalence against toxoplasmosis by latex agglutination test (LAT) and the detection of antigenic particles among seropositive samples by PCR. In the LAT, 118 (22.8%) were positive, and the unadjusted percentage of seroprevalence rates of breeding and fattening pigs were significant difference. Positive rate (14.1%) in the breeding pigs was much lower than that (27.8%) of the fattening pigs (p<0.001, Pearson's Chi-square test). The antibody detection rate of sows was lower than fattening pigs, i.e., 15.8% (25/158) and 26% (93/358), respectively (P=0.011, Pearson's Chi-square test). Among 118 seropositive samples by LAT, 68 (57.6%) were positive in PCR for the detection of the toxoplasma specific-DNA. There was a statistical difference in the positive PCR reaction between the raising pigs(63/93 67.7%) and sows (5/25, 20%) (P<0.01).
Effect of ivermectin and doramectin for larval(Anisakis spp) migratory inhibition in rats
Lee, Seung-Hwan ; Jee, Cha-Ho ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 93~100
This experiment has been investigated in order to examine the effect of ivermectin and doramectin for laval migratory inhibition in rats. Anisakis spp larvae were obtained from the mackerels in fish market in Cheongju. A total of 225 rats were classified with 3 groups with the same number (
rats); group I was control divided into 3 subgroups including 25 rats in each one, others (test groups) were treated subcutaneously with ivermectin and doramectin by 3 kinds of concentrations (
, 400, 800), respectively. At 15 minutes after treating, 10 larvae were taken orally into each rat of 6 subgroups in test, and 5 rats were necropsied at 0.5, 1, 2, 4 and 8 hours after oral administration. The recovered larvae were used in larval migration inhibition (LMI). Ivermectin is significantly more effective than doramectin. In rats, the inhibitory effect of ivermectin and doramectin is slightly less than in vitro.
Protective effects of IgY against diarrhea in suckling piglets -I. Serological result, FA test and RT-PCR-
Jin, Wen ; Yoon, Byung-Il ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 101~111
The purpose of this study was to investigate the protective effects against porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) in suckling piglets by oral administration of IgY. Twenty piglets were divided into two groups with the same number: group I (treated with IgY) and group II (not treated). Group I was administerd orally with IgY for three days from one-day-old and experimentally challenged with PEDV and TGEV at four-day-old. The other was administered with saline solution and challenged with PEDV and TGEV at four-day-old. Serum antibody titers against PEDV and TGEV were examined by enzyme-linked immunosorbent assay (ELISA) and the detection of PEDV or TGEV antigen from feces and small intestines was performed by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescence (IFA). The antibody titers of the group I was higher than that of the other, and lasted at the end of experiment. In the detection tests of both virus from feces and small intestine, the rate of the group I was lower. Based on these results, oral administration of IgY may be effective to prevent the diarrhea caused by PEDV and TGEV.
Protective effects of IgY against diarrhea in suckling piglets -II. Clinical sign, histopathological lesion and immunohistochemical finding-
Jin, Wen ; Yoon, Byung-Il ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 113~128
The purpose of this study was to investigate the protective effects against porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) in suckling piglets by oral administration of the IgY. The piglets were divided into two groups: test and control group. The former (n=10) were administered orally with IgY for three days from one-day-old and experimentally challenged with PEDV and TGEV at four-day-old. The latter (n=10) were administered with saline solution and challenged with same methods. Several tests were studied and summarized as follows; In clinical signs, the piglets of the control group showed the typical signs such as severe watery diarrhea, depression and anorexia but those of the test group recovered progressively. Control group showed 20% in mortality, but there were no death in the other. The gross lesions in the test were milder than those in the control, and there were typical findings as like congestion and distension of lumen in the control group. In histopathological study, the piglets of the control group had shortened and fused intestinal villi and a marked loss of epithelium, whereas the others showed milder changes. It could be concluded that oral administration of IgY, specific yolk-antibody against PEDV and TGEV is effective to prevent PEDV and TGEV infection in suckling piglets.
Analysis of antimicrobial residues in meat samples from slaughtered livestock in 2006
Suh, Dong-Kyun ; Park, Dae-Hyun ; Hong, Ju-Yeon ; Lim, Hyun-Sook ; Kim, Kyung-Hee ; Lee, Chun-Sik ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 129~135
A total of 3,943 samples (beef 698, pork 2,178 and chicken 1,067)were collected from slaughter houses in Daegu from January to December 2006 for analysis of antimicrobial residues. The results were also comparatively analyzed with those carried out from 16 veterinary service laboratories nationwide as a part of national residue program in 2006. The samples from 11 farms (cattle 2, pig 9) were found to exceed the legal limit. Among them 5 were classified as tetracyclines, 3 as
-lactams, 2 as sulfonamides and 1 as quinolone. Nationally a total of 222 farms (241 cases) were found to violate the residual limits, of which 47.3% were detected by screening test, followed by surveillance (38.3%). Failure to observe withholding periods of antimicrobial drugs after treatment was the most significant cause of the non-compliance.
Hematological and biochemical analysis of Korean indigenous cattle according to the ages
Cho, Hyun-Ung ; Ko, Won-Seuk ; Son, Hyong-Won ; Lee, Mi-Jin ; Song, Hee-Jong ; Park, Jin-Ho ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 137~147
Investigations for hematologic and biochemical values and the differential count of WBC for Korean indigenous cattle (KIC)is rarely performed. Therefore, when the index of complete blood counts (CBC) and biochemical analysis of KIC was requested, it had many difficulties to make the results for blood condition since the standard hematologic and biochemical values of KIC are lacking. In the present study, we have investigated the hematologic and biochemical values and the differential count of WBC for blood of total 252 KIC and 17 striped cattle (SC) as a control. As a result, the mean values of RBC and platelet of KIC were significantly decreased by age (P<0.05). The mean values of RBC, HCT, MCV and MCHC between KIC and SC of the same age (2 - 3 years) showed the statistical significance (P<0.05). Also, in the WBC of KIC, the mean values were decreased according to the age from
under 1 year to
over 5 years. In the differential count of WBC of KIC, it showed generally the rates of 45% lymphocyte and 35% segmented neutrophil. In the biochemical analysis, the mean values of BUN and globulin were significantly increased according to the age (P<0.05). Additionally, in comparative analysis between pregnant and non-pregnant group of KIC, the mean values of Hg and HCT in pregnant group were significantly decreased (P<0.05). In conclusion, data obtained from this study may be valuable as a standard for interpretation of the results in hematologic and biochemical analysis of KIC.
Analysis of animal disease control system in Gyeong-In region
Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 149~159
A survey was performed to analyse the current animal disease control system by questionnaires. A questionnaire was administered to the livestock-related experts(n = 116) residing in Gyeong-In region and the livestock farmers(n = 108) residing in Incheon from June to July in 2007. The data was analyzed statistically by SPSS 12.0 version. First, the offices related to the animal disease control were dispersed and their cooperation was not effective. Second, the scale of local disease centers was weaker than that of central one in both man-power(eg. veterinarians), budget and so on, and was not enough to control the outbreak of the main animal diseases. Third, there were also insufficient incentives for livestock farmers to report notifiable animal disease. Finally, there was limitation in bury area and incineration facilities. To develop domestic animal industry, control of the disease and rearing of the environmentally friendly livestock farm are the main directions in Gyeong-In region, Production of high quality and safe livestock products is the most important factor to expand the livestock industry. Conclusively, local animal disease center with comparison to central one should be expanded to control the disease and develop the livestock industry in Incheon metropolitan city.
Degenerative myopathy of the supracoracoideus (DMS) in turkeys and broiler chickens, Review
Song, Hee-Jong ; Lee, Myung-Woo ; Ryu, Kyeong-Sun ; Jang, Hyung-Kwan ;
Korean Journal of Veterinary Service, volume 31, issue 1, 2008, Pages 161~166
Deep pectoral myopathy (DPM), also known as Oregon muscle disease or green muscle disease, was first described in 1968 by Dickinson et al as "degenerative myopathy" in turkeys. Even though this condition was first recognized in adult meat-type turkey and chicken breeders, it is becoming more and more common in meat-type growing birds. DPM occurs exclusively in birds that have been specially selected for breast muscle development. It is generally recognized that DPM is an ischemic necrosis that develops in the deep pectoral muscle (supracoracoideus or pectoralis minor muscle) mainly because this muscle is surrounded by inelastic fascia and the sternum, which do not allow the muscle mass to swell in response to the physiological changes occurring when muscle are exercised, as in wing flapping. The lesion does not impair the general health of birds and is generally found during cut-up and deboning, moreover, it can be both unilateral or bilateral, affecting just one or both pectoralis minor muscle, respectively. No public health significance is associated to DPM, but it is aesthetically undesirable. The fillet should be removed, whereas the rest of the carcass is still fit for human consumption. However, the required trimming operations determine the downgrading of the products and produce an economic loss for the industry, especially because it affects the more valuable part of the carcass. The incidence of DPM increases with market weight in broilers, with more cases reported in higher-yielding strains and in males. Increased bird activity (flock nervousness, flightiness, struggle, and wing flapping) induced by factors such as feed or water outages, lighting programs and intensity, human activity, and excessive noises in and around chicken houses should be looked at as a trigger for the development of DPM in broiler. However, most of the studies conducted to evaluate the incidence of DPM in poultry are concerned with parental commercial breeding stocks under experimental conditions (Bianchi et al. 2006. Poult Sci 85 : 1843-1846). There is a possible genetic relationship between the selection for large-breasted birds and this condition. Management procedures that discourage excessive wing flapping would reduce the incidence (Jordan and Pattison. 1998. Poultry diseases. 398-399).