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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 31, Issue 4 - Nov 2008
Volume 31, Issue 3 - Sep 2008
Volume 31, Issue 2 - Jun 2008
Volume 31, Issue 1 - Mar 2008
Selecting the target year
Detection of the etiological viruses from calves with clinical diarrhea in Gyeongnam south area
Heo, Jung-Ho ; Cho, Myung-Heui ; Lee, Kuk-Cheon ; Park, Mi-Nam ; Cho, Eun-Jeong ; Choi, Man-Su ; Kim, Chung-Hui ; Kang, Joung-Boo ; Kim, Eui-Kyung ; Kim, Jong-Shu ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 265~272
Among calves' diseases, digestive diseases most frequently occur in Korea, and especially viral diarrhea is one of the most important diseases. This study was conducted to get some basic information for the control of the viral diarrhea in calves. The samples were obtained from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006, Viral detection was examined by polymerase chain reaction (PCR), Etiological viruses were detected from 171(53,6%) of 319 calves, and bovine rotavirus (BRV) were 130 (40,8%) and bovine viral diarrhea virus (BVDV) 41 (12,9%), and no coronavirus was confirmed, Statistical difference was found in BRV detection between summer (32.6%) and winter (57.7%). However there was no seasonal difference in BVDV. In detection rate of the calves under 19days, BRV was highest (55.1%), but BVDV lowest (5.1%). No big difference was in rate among herd size. However, BRV was lowest (26.8%) in the group over 51 heads, but BVDV was highest (19.5%) in the same.
Investigation of carprine arthritis-encephalitis from dairy goat in Jeonnam province
Lim, Jong-Soo ; Kim, Hui-Joung ; O, Hyun-Yi ; Lee, Tea-Uk ; Park, Suk-Jun ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 273~281
This survey was conducted to investigate the infection of caprine arthritis encephalitis virus (CAEV). From April to October 2007, we examined a total of 173 goats in 7 dairy goat-breeding farms located in Na-ju, Hae-nam, Young-am, and Young-gwang area of Jeonnam province. The results show that 41 (23.7%) goats are positive for CAEV, confirmed by nested PCR of 173 blood samples. Regional analysis revealed that a positive proportion for CAEV was 50.0% in Young-gwang, 25.6% in Young-am, 25.0% in Hae-nam, and 10.9% in Na-ju. Clinical signs were observed in 17 (9.8%) goats, of which 6 suffered from arthritis, 7 from mastitis, and 4 from pneumonia. Among the examined 173 goats, 8(4.6%) goats are positive for CAEV accompanied with CAE symptoms. There was a tendency to be much higher(p<0.05) levels of natrium(Na), chlorine(Cl) in CAEV-positive than in CAEV-negative goats. However, other serum biochemical values were no statistically significant effect.
Occurrence of highly pathogenic avian influenza from domestic ducks in Jeonbuk province
Chu, Keum-Suk ; Kang, Mi-Seon ; Cho, Bum-Jun ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 283~290
Highly pathogenic avian influenza(HP AI) is one of the most important zoonoses in the around of world. That occurred in the domestic ducks of 34 olds day in Jeonbuk of Korea on April 2008. All of infected ducks were died. We could found several clinical signs such as facial swelling with cloudy eyes, conjunctivitis, and neurological sign. In the autopsy, moderate congestion in the kidney, multifocal hemorrhagic foci in pancreas, and mild swelling in brain were observed. Gliosis in brain and severe congestion in kidney were also shown in histopathological findings. Specific band for H5 antibody was detected in polymerase chain reaction (PCR).
Evaluation of vaccination against Mycoplasma hyopneumoniae in pigs at different vaccination time-points
Lee, Ham-Hee ; Rha, James ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 291~303
This study was performed to evaluate the effect of commercial swine mycoplasma vaccine (M+
, Schering-Plough Animal HealthCo.) at different vaccination time points. Total 139 piglets were moved to experimental farm after weaning and were randomly allocated to 3 treatment groups and 1 control group. Piglets of 3 treatment groups (A, B and C) were vaccinated twice at 1 and 3 weeks (A group), 3 and 5 weeks (B), and 6 and 8 weeks (C), respectively. All vaccinates showed higher antibody titers compared with nonvaccinates, and maintained to finish (P<0.001). Performance and RBC/WBC count showed no significant difference between groups. Vaccinates at 6-8 weeks showed better effect on decrease of clinical sign compared with vaccinates at 1-3 weeks and 3-5 weeks and nonvaccinates. Also, lung lesion assessment showed significant difference between vaccinates at 3-5 weeks and 6-8 weeks, and nonvaccinates and vaccinates at 1-3 weeks (P<0.05). Vaccinates at 3-5 score in comparison with other groups. In conclusion, these results suggested that vaccination at 3-5 weeks or later may be more effective than earlier vaccination.
An epidemiological study on the calves with clinical diarrhea in southern Gyeongnam
Heo, Jung-Ho ; Cho, Myung-Heui ; Lee, Kuk-Cheon ; Park, Mi-Nam ; Cho, Eun-Jeong ; Choi, Man-Su ; Kim, Chung-Hui ; Kang, Joung-Boo ; Kim, Eui-Kyung ; Kim, Jong-Shu ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 305~313
The present study was conducted to investigate the epidemiological characteristics such as isolation rate and antimicrobial drug susceptibility of etiological agents. The specimens (stool or intestine) were collected from 319 calves with clinical diarrhea from 195 farms in Gyeongnam south area (Gosung, Tongyung, Hadong) from June 2005 to August 2006. The isolation rate of Salmonella spp was higher in summer (8.4%) than in winter (4.8%) and the average was 7.2% (23/319 head). Some of Salmonella spp isolated were resistant to penicillin, oxytetracycline, tetracycline, and cephalexin (>90%), but some of them were susceptible to norfloxacin, ciprofloxacin, sulfamethoxazole/trimethoprim and amikacin(>30%). There was no statistical difference in the isolation rate of Eimeria spp between summer(48.9%) and winter(42.3%). For the evaluation of infection level of Eimeria spp oocyst per gram of feces (OPG) was examined, and severe, moderate and light infection level were 11.9%, 12.5% and 22.3%, respectively. In the isolation rates of Eimeria spp the calves under 19 days was lowerthan those over 60 days, but there was not different among herd size.
A comparison of agglutination tests and enzyme-linked immunosorbent assay for the bovine brucellosis
Kim, Cheol-Ho ; Bang, Sang-Young ; Jeon, Jae-Hyung ; Bhak, Jong-Sik ; Lee, Min-Kwon ; Shin, Jung-Sub ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 315~329
A total of 710 bovine serum samples which are composed 532 bovine serum samples showed negative reaction and 178 bovine serum samples showed positive reaction with tube agglutination test (TAT) from North area of Gyeong-nam, Korea were tested using all the 3 assays which are Rose-Bengal test(RBT), tube agglutination test (TAT) and enzyme-linked immunosorbent assay (ELISA, two types) and analyzed for evaluation of specificity, sensitivity, reproducibility and predictive value. In the comparison of serum antibody titer agglutination test, RBT showed almost agreement with TAT. In the comparison of TAT and two types of ELISA method, they showed difference in specificity and sensitivity about 5%. But there is no significant difference in detecting sensitivity between two types of ELISA method and TAT. In serologic tests for bovine brucellosis, the new assay ELISA would be a good candidate for serologic survey for bovine brucellosis in Korea because it is efficient in detecting many test samples quickly. But the serum agglutination tests (RBT, TAT) are more economical and easy assay for detection. In the test of comparison of antibody titer between first day of finding and 10 days after finding by TAT, there was no change in 55% (76/139) of positive cattle.
Comparative serology of human brucellosis in Korea
Lee, Sung-Il ; Choi, Min-Jung ; Hur, Jin ; Islam, Md Ariful ; Khatun, Mst Minara ; Baek, Byeong-Kirl ; Lee, Chang-Seup ; Kakoma, Ibulaimu ; Bolye, Stephen M ; Srirangananathan, Nammalwar ; Young, Edward John ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 331~338
The study was carried out to evaluate the diagnostic efficacy of the tube agglutination test (TAT), enzyme-linked immunosorbent assay (ELISA) and the 2-Mercaptoethanol agglutination test (2-MAT) to detect human brucellosis patients in Korea. We examined 87 serum samples of people in the rural farm areas where bovine brucellosis had been reported. People in this study were divided into seven groups- farmers and their families, veterinarians, veterinary quarantine workers, livestock health control officers, artificial inseminators, livestock traders and healthy control individuals. Among 87 people, 65 were males and 22 were females ranging in age from 13 to 72 years. Of 87 serum samples, ELISA detected 21.84%, TAT detected 11.50% and 2-MAT detected 8.05% Brucella positive sera. Brucella specific IgG ELISA antibody titer was recorder higher in the individuals between the ages of 50 and 65 years. The highest prevalence rate of brucellosis(29.4%) was recorded in the cattle farmers and their family members followed by quarantine veterinary office workers (25%) and practicing veterinarians 01.1%). The majority of the Brucella sero-positive individuals in this study had a history of direct contact with animals.
A study on the prevalence of Riemerella anatipestifer from domestic ducks in Cheonan-Asan city
Kim, Sue-Jung ; Kang, Su-Jung ; Yook, Sim-Yong ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 339~345
This survey was carried out to investigate the infection rate of Riemerella anatipestifer from domestic ducks by isolation. A total of 145 samples (nasal swabs and carcasses) were collected from farms to examine the biochemical properties and antimicrobial susceptibility test from November 2006 to February 2008. Riemerella anatipestifer was isolated from 13 of 145 sample and isolation rate was higher in the ducks below 25 days (76%, 10/13 isolates) than those over 26 days (23%, 3/13). However, there was no significant difference in isolation rate between region. The biochemical properties of isolate were Gram negative (-), non-fermentative rod that grows fastidiously on routine media. In the test of antimicrobial drug susceptibility the isolates were susceptible to tetracycline(100%), enrofloxacin (77%), ampicillin (70%).
on protection against PMWS with
Ahn, Na-Kyoung ; Seo, Tae-Won ; Jeong, Hyun-Kyu ; Yoon, Byung-Il ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 347~356
The purpose of this study was to evaluate the efficacy and cross-protection of serovar 12 against serovar 4 or 5 of H parasuis with M+
. A total of 141 piglets from 2(A and B) farms were used and divided into experimental group and control group in each farm. Farm A has been detected H parasuis serovar 12, whereas farm B has been detected H parasuis serovar 4 or 5 from post-weaned pigs with PMWS. The piglets were vaccinated intramuscularly with 2.0ml of M+
in experimental group or normal saline in control group at 1 week of age. A same booster dose was given at 3 weeks of age. In order to value the antibody titer to H parasuis using by tube agglutination test, blood samples were collected from piglets at the aged of 1 week, 6 and 14 weeks. In experimental group and control group, the average antibody titers were
at the aged of 1 week, 6 and 14 weeks, respectively. The cumulative clinical sign indexes by standard guideline of Schering-Plough Animal Health Corp were 486 and 1,069, respectively. The average daily gains and feed conversion rates were
, and 1.99 and 2.24, respectively. The average gross lesion scores were
, respectively. According to these results, the M+
containing H parasuis serovar 12 may be induce circulating antibodies that cross-react with serovar 4 or 5 and have a protection of PMWS with H parasuis.
Infection rate of parasites from feces of Korean indigenous goats in northern areas of Gyeongbuk province
Cho, Kwang-Hyun ; Lee, Ji-Hong ; Kim, Dae-Jung ; Kim, Seung-Joon ; Kwon, Oh-Deog ; Kwak, Dong-Mi ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 357~362
While studies have been carried out on endoparasite infection from feces of Korean indigenous goats in multiple areas around the nation, there is no report in northern areas of Gyeongbuk province. Thus, this study was conducted to examine the rate of parasite infection from the fecal samples of goats raised on grass. From March to July in 2008, 108 fecal samples were taken from goats. Eggs of parasites were identified using flotation or sedimentation methods followed by microscopic examination. Overall infection rate of parasites from feces of goats were 99% (107 out of 108). The infection rates of nematode, cestode, trematode and protozoa were 37%, 23%, 0% and 99%, respectively. Among parasite eggs detected, nematodes included threadworms (20%), strongyles (16%) and whipworms (1%). The only cestode and protozoa detected were Moniezia expansa and Eimeria spp, respectively. In the rates of mixed infection, single was 61%, double 32%, and triple 6%.
Investigation of Neospora caninum infection in Korean indigenous Cattle in Uljin
Chon, Ryoung-Hoon ; Jang, Young-Sul ; Lee, Eun-Mi ; Choi, Jung-Hye ; Park, No-Chan ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 363~367
This study was carried out to investigate an epidemiological state of neosporosis in Korean indigenous cattle in Uljin. Bloody samples were collected from 552 female cattle (337 farms), more than 5 years old, in 10 districts of Uljin, Antibody to N caninum were examined by enzyme linked immunosorbent assay (ELISA). Seroprevalence of individual and farm were 7.6% (42/552) and 8.6% (29/337), respectively. Positive rates by districts was variable
, and it was seemed to be a tendency that positive farms gathered with in a small zone. In seroprevalence by herd size farms having between 5-9 heads was top (37%), but there was no significant difference among herd size.
Analysis of Melanocortin receptor 1 (MC1R) gene differential test for beef species between Hanwoo and Holstein using polmerase chain reaction -restriction fragment length polymorphism (PCR-RFLP)
Suh, Dong-Kyun ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 369~374
The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.
Discrimination of cows from heifers by antemortem and postmortem inspection in slaughterhouses (II)
Choi, Im-Yong ; Choi, Tae-Suk ; Lee, Dal-Ju ; Lee, Hae-Yeong ; Kim, Yeon-Ha ; Lee, Kyung-Hye ; Rho, Chang-Sik ; Kim, Kyu-Hiun ; Kim, Eun ; Sin, Bang-Woo ; Lee, Jung-Hark ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 375~384
This study was performed to establish the discrimination of the corpus albicans that were formed after normal parturition and abortion in Holstein, The corpus albicans were obtained from 504 cows and 506 heifers slaughtered in the Agricultural Cooperative's Livestock Products Joint Market of Seoul City from March 2007 to October 2007. The difference in a gross examination (morphology, fibrogenesis, deposit state, color, and size) among the fixtures that exist in their ovaries was investigated. All of the cows had corpus albicans in their ovaries: 423 cows (83.9%) had less than three corpus albicans, 81 (16.1%) had more than four. In the heifers, however, 463 (91.05%) had no corpus albicans, 39 (7.7%) had one, and four (0.8%) had more than two. In the types of corpus albicans 73.2% of the cows had oval-type, 14.4% had circular-shaped, 12.4% had irregular-type. In the aborted heifers, however, oval-type appeared in 88.6% and circular-shaped appeared in 11.4%, and no irregular-type was found. There was no significant difference in the fibrogenesis and size of corpus albicans between cows and aborted heifers. However, slight difference in the color of the corpus albicans was observed between two types. In conclusion, it is not easy to discriminate by gross examination of ovary between cows and aborted heifers in the slaughter house.
A study on the residues of fluoroquinolones in hen's eggs consumed in Incheon Metropolitan city
Park, Eun-Jeong ; Lim, Ji-Huen ; Lee, Sung-Mo ; Im, Jeong-Soo ; Oh, Dae-Kyu ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 385~395
This study was carried out to investigate the residue level of fluoroquinolones in hen's general eggs and specific eggs by microbiological assay method and high performance liquid chromatography (HPLC) method. HPLC separation was carried out by reversed phase chromatography on a Symmetry
particle size) with a phase composed of distilled water (containing 0.4% triethylamine and phosphoric acid) : Methanol (780 : 220, v/v), pumped isocratically at a flow rate of 1.0ml/min. A fluorescence detector was utilized with an excitation wavelength of 278nm and an emission wavelength of 456nm. The calibration curves were linear
over a concentration range of
. Average recoveries of the five fluoroquinolones in whole eggs at fortified levels of
were ranged mean
and low coefficient of variation was less than 10% for all analysed samples. The limits of detection and limits of quantification for whole eggs were
, respectively. Only one hen's general eggfrom chicken farm in Incheon was detected with the residual fluoroquinolones (Microbiological assay method; 1 of 47 general eggs) ; the range of residual concentration enrofloxacin was 0.12ppm. Those in food stores were detected with the residual fluoroquinolones (Microbiological assay method; 4 of 88 general eggs) ; the ranges of residual concentration enrofloxacin were
, and hen's specific eggs (40) in food stores were not detected. For the microbiological assay method of fluoroquinolones in hen's eggs, as the results of comparative analysis, the disc diffusion method with E coli may be a little highly detected for the residual fluoroquinolones.
A study on the early pregnancy diagnosis by changing of plasma progesterone concentration and morphology of ovary in pregnancy and non -pregnancy cows
Kim, Cheol-Ho ; Bhak, Jong-Sik ; Shin, Jung-Sub ; Kang, Chung-Bo ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 397~414
In order to evaluate conception rate of Hanwoo in northwestern region of Gyeongsang-nam-do, we investigated conception rate and reduction of reproductive disorder rate after artificial insemination (AI) in 1,000 heads of breeding cows, This study showed that 80.9% of cows were classified as fertility after 1st and 2nd AI. For a accurate pregnancy diagnosis with practicing ovariectomy and histeotomy, we comparatively investigated each of 80 slaughtered cows, including 30 of non-pregnancy, and used enzyme-linked immunosorbent assay (ELISA) for estimation of plasma progesterone concentration and serum luteal hormone. The mean diameter of non-pregnant corpus luteum is
and that of pregnant corpus luteum is
. This indicates that corpus luteum is more developed in the ovary of pregnant than non-pregnant cows (P<0.05). The diameter of pregnant corpus luteum according to the stage of pregnancy showed
in early stage (1-3 month),
in middle stage (4-6 month) and
, in last stage (7-9 month). This indicates that corpus luteum in middle and last stage is more significantly developed than that of early stage(P<0.05). The mean plasma progesterone concentration of cows showing size of non-pregnant corpus luteum was
and that of pregnant corpus luteum
. Thus, it was more significantly increased in pregnant corpus luteum(P<0.02).. However, it was low to
. in estrus (corpus albicans). The plasma progesterone concentration according to gestation period was high in proportion to the degree of development in corpus luteum and more significantly increased (P<0.05) and maintained in middle and last state than early state. The concentration was sharply decreased to
at parturition. As a consequence, we can practice the early pregnancy diagnosis by confirming non-pregnancy when the mean plasma progesterone concentration is below 1ng/ml 19 to 22 days after AI and this can be available to diagnose reproductive disorder.
Effects of noise stress on growth performance and serum profile of Korean indigenous goats
Cho, Kwang-Hyun ; Baek, Yong-Jin ; Kim, Byung-Ki ; Lee, Ji-Hong ; Kim, Yeong-Hwan ; Kim, Sung-Kuk ; Kwon, Oh-Deog ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 415~423
This study examined the effects of the daily noise stress on body weight, feed intake and clinicopathological findings in Korean indigenous goats. A total of 14 male goats was divided into 2 groups (test and control) with same number (n=7), and exam was started 2 weeks later. This study composed 2 stages. First was noise stage i.e., noise (80-100 dB) inflicted on the test for 70 minutes each time (5 times/day), and have continued for 2 months. Second was no noise for 2 months. Body weight and daily gain in stress group was decreased compared with control group after imposing noise (first stage). However, these results were reversed after ceasing noise (second stage). Average daily feed intake was not significant different between two groups throughout the experimental periods. But, feed conversion ratio was lowerin control group than another. Clinicopathological findings including total protein, albumin, globulin, total bilirubin, total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, amylase, blood urea nitrogen (BUN), cortisol, total leucocytes and hemoglobin value showed no significant different between stress and control group.
Efficient management of zoonoses
Park, Jae-Myoung ; Ryu, Jae-Yoon ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 425~431
A strategic national plan to control of zoonoses is currently somewhat insufficient. Government should extensively assign all case reports of zoonotic disease outbreaks and needs to control them in the context of other decrees. Additionally capacity of veterinarian needs to he increased to control zoonotic diseases efficiently. Namely, veterinarian is responsible for reporting to health authorities if any of zoonotic diseases are diagnosed. Furthermore, government should deploy the veterinarian's resources for prompt epidemiological investigation and for taking countermeasures against zoonotic diseases.
Prevalence of brucellosis and its association with reproductive problems in goats in Bangladesh
Rahman, Md Siddiqur ; Uddin, Muhammad Jasim ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 31, issue 3, 2008, Pages 433~438
Brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. Brucella species infection is endemic in human beings and livestock in Mediterranean countries, and it is also present in Asia, sub-Saharan Africa and Latin America. The importance of brucellosis is not known precisely, but it can have a considerable impact on both human and animal health, as well as having socioeconomic effects, especially in areas where rural income relies largely on livestock breeding and dairy products. Brucellosis in human beings is caused by exposure to livestock and livestock products. Infection can result from direct contact with infected animals and can also be transmitted to consumers through raw milk and milk products. In human beings, the symptoms of disease are weakness, joint and muscle pain, headache, undulant fever, hepatomegaly, splenomegaly and night sweats. Recently, it has been reported that brucellosis can affect the central and peripheral nervous system. In animals, brucellosis mainly affects reproduction and fertility, reduces the survival of newborns and reduces milk yield. The disease does not cause significant mortality in adult animals. In Bangladesh, approximately 80 percent of people live in villages, and rural income is largely dependent on livestock; the people are in close contact with livestock on a daily basis. There are about 33.55 million goats