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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 31, Issue 4 - Nov 2008
Volume 31, Issue 3 - Sep 2008
Volume 31, Issue 2 - Jun 2008
Volume 31, Issue 1 - Mar 2008
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Differentiation of mouse embryonic stem cell into smooth muscle cells by DBcAMP and retinoic acid
Park, Sung-Soo ; Kang, Ju-Won ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 449~456
The differentiation of mouse embryonic stem(ES) cell into smooth muscle cells(SMC) may play a major role in cardiovascular development and under pathophysiological conditions. Therefore, in the present study, we have examined the differentiation of ES cells and its related gene expression. SMC differentiation was indicated by cellular morphology and time-dependent induction of dibutyryl adenosine 3,5-cyclic monophosphate(DBcAMP)and retinoic acid(RA) on smooth muscle
), smooth muscle myosin heavy chain(SMMHC) gene expression. The control was undifferentiated ES cells(protein expressions represent 50-60kDaOct-4). The results of this study show that morphology of embryoid body and confirmation of
expression by immunocytochemistry. Moreover, SMMHC and desmin expression was significantly increased by time dependent manner(5, 7, 15 days), in contrast to
expression was slightly decreased on 15days. In conclusion, DBcAMP and RA stimulate mouse ES cells differentiation into SMC and enhanced
, SMMHC and desmin expression.
Effect of estradiol-
on proliferation in primary cultured chicken hepatocytes
Baek, Gyul ; Kang, Ju-Won ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 457~463
The sex steroid hormone estradiol-
mediate their biological effects on development, differentiation and maintenance of reproductive tract and other target tissue through gene regulation by nuclear steroid receptors. Although the importance of
in many physiological process has been reported, but little is known about the effects of
on primary cultured chicken hepatocyte. therefore, in the present study, we have examined the effect of
on cell proliferation and it's related signal cascades.
-thymidine incorporation in time-(
) and dose-(
)dependent manner and treatment of
increased the phosphorylation of p44/43 MAPKs(p44/42 mitogen-activated protein kinase) and JNK(c-Jun N-terminal kinase) in a time dependent manner. In addition, PD98059(p44/42 blocker,
), SP600125(JNK blocker,
) blocked the estrogen-induced increase in
-thymidine incorporation. In conclusion,
stimulates the proliferation of primary cultured chicken hepatocytes and this action is mediated by p44/42 MAPKs and JNK signal transduction pathway.
Sarcocystosis in a 30 month old Hanwoo(Bos taurus oreanae)
Ku, Kyung-Nyer ; Kim, Kyung-Sook ; Yang, Il ; Lee, Ho-Seung ; Woo, Jong-Tae ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 465~469
Unusual yellowish-green intramuscular granulomatous lesions were found in a carcass of Hanwoo(slaughtered, 30 month old). Those were 1-3 mm in diameter, oval shaped, and paralleled with muscle fibers. Histologically, severe inflammation, eosinophilic granulomas and necrosis were observed in the muscle tissue. We also observed sarcocysts in the muscle cells. In a polymerase chain reaction, we identified 900bp length, sarcocystis specific fragment. It would be diagnosed as sarcocystosis in Hanwoo.
Spinal cord abscess in pork carcasses
Seo, Ji-Yon ; Ryu, Jae-Yoon ; Park, Jae-Myoung ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 471~473
This is a case report of spinal cord abscess in pork carcasses. We have detected 7 cases with spinal cord abscesses in abattoir from January to October 2008. It may occur from either direct extension or hematogenos infection. Four cases were presumed by hematogenous infection because of no external wound, and 3 may be caused by direct extension. The infection is a result of external wound in 3 pigs and is a result of hematogenous spread in 4 pigs. The incidence rate of spinal cord abscess in sows is higher than that in pigs.
Occurrence, clinical signs, postmortem lesions and etiology of enterotoxaemia in Black Bengal goats
Islam, K.B.M. Saiful ; Rahman, Md. Siddiqur ; Ershaduzzaman, Md. ; Taimur, M.J.F.A. ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 475~484
A year round study was carried out to investigate the etiology, clinical signs, postmortem lesions and occurrence of naturally occurring enterotoxaemia in Black Bengal goats. Sixteen goats of different age and sex died in different seasons with sigh associated with enterotoxaemia made the materials of this study. Accidental access to large amount of concentrate was noted as one of the predisposing factors although few cases were reported to occur without known diet change. Younger animals(50%) and males(62.50%) were found more prone to the disease and it was likely to be more prevalent during winter(50%) followed by at rainy season(31.25%) and summer(18.75%). Diarrhoea(81.25%), dullness(56.25%), drooping of the ears(50%), anorexia(43.75%) were recorded as major clinical signs whereas enterocolitis(100%), lung edema(87.50%), fluid filled intestines(87.50%), enlarged mesenteric lymph nodes(56.52%) etc. were most common post mortem lesions found. A few cases showed lesions on heart(31.25%), brain(25%) and/or liver/spleen(18.75%) but no lesion was found on kidney. Thus the so called 'pulpy kidney' lesion was absent. Intestinal contents were subjected to conventional bacteriological culture based methods to identify the causal agents. Based on the morphological, cultural and biochemical properties the causal agent was identified as Clostridium perfringens. Despite the study was carried out at certain area it showed a clear picture of goat enterotoxaemia in terms of etiology, clinical signs, postmortem lesions and occurrence of goat enterotoxaemia in Bangladesh.
Comparison of two diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR to discriminate between Salmonella enterica serovars Gallinarum and S Pullorum
Lee, Se-Mi ; Seo, Ja-Young ; Lee, Jae-Il ; Kim, Tae-Jung ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 485~492
Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR over 2 single nucleotide polymorphisms (
). In both methods,
allele was found to be a good target for differential diagnosis, while
allele produced some non-specific reactions.
Tube agglutination test is superior than other serological tests for diagnosis of brucellosis in small ruminants
Rahman, Md. Siddiqur ; Jahan, Nusrat ; Hossain, Mohammad Arif ; Uddin, M.J. ; Shil, Niraj Kanti ; Islam, KBM Saiful ; Ahasan, Md. Shamim ; Rahman, A.K.M. Anisur ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 493~496
Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants(300 goats and 62 sheep) were examined. Approximately 3-5 ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.
Prevalence and risk factors of subclinical bovine mastitis in some dairy farms of Sylhet district of Bangladesh
Kahir, Md. Abdul ; Islam, Md. Mazharul ; Rahman, A.K.M. Anisur ; Nahar, A. ; Rahman, Md. Siddiqur ; Son, Hee-Jong ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 497~504
A cross-sectional study was undertaken to report prevalence and to identify risk factors of subclinical mastitis of dairy cattle in Sylhet district of Bangladesh. Among 325 dairy farms of the district 12 farms(3.7%) were selected conveniently for this study. All the dairy cows of the 12 farms were selected for sample collection. Fresh milk samples from each of the selected dairy cows were collected aseptically in separate sterilized test tube as RF, RH, LF and LH quarter of the udder. Rapid modified White Side Test(WST) was used to detect subclinical mastitis(SCM). Results of WST and data derived from filled in questionnaire were entered in Microsoft Excel 2003 and transferred to
, version 8.0/Intercooled(Stata Corporation, Texas, USA, 2003). The overall prevalence of SCM and its distribution in different categories of variables in cow and their exact binomial 95% confidence intervals were calculated in
. Simple bivariable associations among independent variables were investigated by
. Multiple logistic regression analysis with backward elimination method was used to identify risk factors of SCM. To identify significant variation in quarter SCM, linear regression analysis was performed after arcsine transformation of the data. The overall prevalence of SCM found in this study is 54%. Dairy cows with teat lesions had significantly increased SCM(OR=12342, P value=0.000, 95% CI=762, 199798) than others without teat lesions. The Holstein Friesian X Jersey X Sahiwal breed has significantly decreased(OR=0.18, p=0.03, 95% CI 0.04, 0.85) SCM than other breeds. The prevalence of SCM found in this study is in agreement with others. The injury in the teat increases the probability of getting infected with microbes and thereby mastitis. If the prevalence of teat lesion can be decreased the probability of subclinical mastitis will also be decreased. The negatively associated Holstein Friesian X Jersey X Sahiwall breed may help in planning mastitis control program if this finding can be validated by a more powerful case-control or cohort study design.
Comparison of diagnostic methods on failure of passive immunoglobulin transfer to Korean-indigenous calves
Lee, Byoung-Seok ; Kang, Mun-Il ; Chung, Yong-Un ; Lee, Chai-Yong ; Han, Dong-Un ; Wee, Sung-Hwan ; Yoon, So-Rah ; Cho, Jae-Jin ; Kang, Ju-Won ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 505~519
For screening the appropriate field diagnostic techniques to failure of passive immunoglobulin transfer(FPT) in Korean-indigenous calves, 258 sera was examined by spectinophotometry for total protein(TP) and globulin(Glo), sodium sulfate precipitation test(SSPT), zinc sulfate turbidity test(ZSTT), and single radial immunodiffusion test(sRID). All calves aged within 6-week old. Morbidity and mortality to various diseases, mainly including enteric and respiratory disorders, were 18.9%(49) and 4.2%(11), respectively. FPT was 27,9%(72/258) when the cutoff point of TP was
and among them the morbidity and mortality were 27.9% and 6.9%, respectively. FPT was 29.1%(75/258) when the cutoff point of Glo was
and among them the morbidity and mortality were 29.0% and 6.9%, respectively. FPT was 13.1%(34/258) when the cutoff point of SSPT was 1+ and among them the morbidity and mortality were 67.6% and 23.5%, respectively. FPT was 19.7%(51/258) when the cutoff point of IgG with sRID was
and among them the morbidity and mortality were 41.1% and 11.7%, respectively. In addition, mean concentration of IgG with sRID tested was
at 3-day old but
at 9-days with
. The results of the study were suggested that SSPT for FPT was the relatively reliable and convinient method for evaluating the immune status of calves(P<0.05).
Study on disease prevalence to Holstein calves reared in Chonnam area
Lee, Sang-Hoon ; Kang, Ju-Won ; Chung, Yong-Un ; Lee, Chai-Yong ; Han, Dong-Un ; Wee, Sung-Hwan ; Yoon, So-Rah ; Cho, Jae-Jin ; Kang, Mun-Il ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 521~532
The prevalence of major calf disease was investigated in 117 Holstein dairy calves in Chonnam area. All of them were moved in the College experimental farm which is operated in intensive units. clinical signs were daily examined throughout two months after the introduction of the College farm. Among calves, 92 cases(78.6%) died in the two months after the introduction in it. Outbreaks of respiratory and alimentary diseases were their main causes of their fatality. The incidence of respiratory disorders during the full period of the experiment was up to 42.8%, and the alimentary diseases were occurred 35.9% of the herd. Most of the mortality was related with respiratory(59.9%) and alimentary(52.1%) pathogens. Also calf mortality by combined infection claimed 6.6% among 100 morbidity cases. Principle pathogens to cause mortality were Pasteurella spp(44.4%), E coli(29.9%), bovine viral diarrhea virus(16.2%), IBRV(12.0%), respectively. Viruses also played as an important role in increasing calf morbidity to secondary respiratory bacterial pathogens. Pasteurella infection combined with infectious bovine rhinotracheitis virus(11 cases), parainfluenza virus type-3(9 cases), or bovine respiratory syncytial virus(7 cases) was appeared as major pattern to mortality. colibacillosis in causing enteritis was concurrently infected with BVD(19 cases), bovine coronavirus infection(14 cases), salmonellosis(5 cases), coccidiosis(5 cases) and clostridial infection(4 cases). Ninty-two cases to death were appeared to have 100% neutralizing antibodies to BCV; Among them, 73.8% had the neutralizing antibody level higher than 64. Calves with neutralizing antibodies higher than 16 to BVDV were 50%. The cases with neutralizing antibody level lower than 8 to BEFV were 89.4% that means the necessity of appropriate vaccination.
Studies on immunotherapy to calves with failure of colostrum passive transfer using bovine immune sera
Lee, Jung-Sun ; Kang, Mun-Il ; Chung, Yong-Un ; Lee, Chai-Yong ; Han, Dong-Un ; Wee, Sung-Hwan ; Yoon, So-Rah ; Cho, Jae-Jin ; Kang, Ju-Won ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 533~546
The efficacy of bovine immune sera to correct the calves with failure of passive transfer(FPT) was evaluated. Immune sera were produced from 14 one-year-old Holstein cattle which were inoculated commercial combined viral vaccine, administered by the challenge of some main enteric or respiratory viruses, aseptically filtered and stored at
before used. After the treatment of bovine immune sera, Mean transfer factor(
, of IgG administered/kg of body weight) was
in K-IP, H-IP and K-IV group, respectively. The corrective effect of bovine immune sera to FPT calf without any clinical signs showed that intravenous route was more effective than intraperitoneal administration(P<0.01). FPT calves with severe signs were not effective response to the immunotherapy used and consequently died within 10 days after the treatment. Ten percentage of controls appeared the clinical signs including diarrhea. On the contrary, there were not any clinical signs in K-IP and H-IV group. There was significant increase of the neutralizing titer against bovine viral diarrhea virus and bovine coronavirus as well as of cell population including CD2, CD4, and monocyte in K-IP and H-IV group after the immunotherapy(P<0.05). Also, K-IP and H-IV group showed the successful correction to FPT within one week after the immunotherapy, but controls had kept the FPT two-four weeks even after the same treatment. Consequently, the results were suggesting that the bovine immune sera could be used the corrective tool to young calves with FPT.
Identification of bacterial contaminants in porcine semen and its removal
Park, Choon-Keun ; Hong, Ki-Hun ; Lee, Yong-Seung ; Hahn, Tae-Wook ; Son, Su-Jung ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 547~554
Bacteriospermia is a frequent finding in fresh boar semen and can result in detrimental effects on semen quality and longevity. The objectives of this study was to evaluate types of bacterial contaminants in porcine fresh semen and the reducing effect of antibiotic and density gradient with percoll on the bacterial contaminants. Fresh semen was collected by gloved-hand method into a pre-warmed(
) thermostable bottle, and was inoculated onto blood agar and MacConkey agar, respectively. After incubated for 48 hour, 7.5%
, bacterial colonies were selected and identified by Gram staining, oxidase test, catalase test and finally identified using API kits and Vitek system. Aerobic culture yielded a variety of bacteria from different genera. The most prevalent contaminant of fresh semen were Leclecia adecarboxylata, Acineobacter banmanni, Staphylococcus epidermidis, Staphylococcus cohni spp urealyticus, Proteus mirabilis. Most of identified bacteria were Gram(-) and non-pathogenic bacteria. It seems that bacterial contaminants in fresh semen were seem originated from multiple sources at the stud/farm, and were from animal and non-animal origins. Gentamicin treatment did not eliminate the bacterial contaminants completely but 3 step-density gradient with percoll completely removed the bacterial contaminants in fresh semen. Therefore, future study is necessary to prove that density gradient method with percoll can eliminate bacteria in fresh semen without significantly affecting sperm viability or function.
Effects of feeding medicinal herbs on growth performance and carcass quality in finishing pigs
Kim, Sang-Uk ; Jung, Ji-Yeong ; Park, Sang-Guk ; Jo, Sang-Sin ;
Korean Journal of Veterinary Service, volume 31, issue 4, 2008, Pages 555~566
This study was performed to evaluate the effects of medicinal herbs on characteristics and qualities of pork for 6 months using 102 post-weaning piglets, To improve the bod gain and feed efficiency rate, post-weaning piglets were fed with medicinal herbs from 4 to 10 weeks after birth, while grower pigs for 60 days before slaughter. Daily body gain of piglets of experimental group was higher(493.1g) than control(447.8g), with feed demand index 1.49 versus 1.6 times in control, indicating. The more pigs were fed with herbs, the higher body gain was observed. Among carcass characteristics, dressing percentage and backfat thickness were higher in experimental group(68.26% and 14.52mm) than control(67.39% and 14.36mm). Experimental group(0.5% medicinal herb-fed) produced more A class carcass. In fatty acid analysis, meats of experimental group contained less saturated(more unsaturated) fatty acids than control. Especially, palmitic acid, a precursor of cholesterol, was less and oleic acid was more contained than control. In amino acid analysis, essential amino acids(methionine and isoleucine) were increased in meats of experimental group. In concolusion, feeding medicinal herbs has effects such as increased body gain, improved feed demand, and carcass class on piglets and improved meat quality on growers.