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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 32, Issue 4 - Dec 2009
Volume 32, Issue 3 - Sep 2009
Volume 32, Issue 2 - Jun 2009
Volume 32, Issue 1 - Mar 2009
Selecting the target year
Genetic characterization and phylogenetic analysis of porcine circovirus type 2 field strains isolated from Korean pocine circovirus disease (PCVD) pigs
Jin, Wen ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 1~10
In order to obtain the genetic information of the Korean isolates of porcine circovirus 2 (PCV2), complete genomes of five isolates from Korean PCVD weaned pigs with wasting syndromes were sequenced and compared with those of other published PCV2 isolates. Of the five PCV2 isolates, four (1767 nucleotides) were classified into PCV2b, and one (1,768 nucleotides) was PCV2a. Moreover, it appeared that PCV2b is now the dominant genotype circulating in Korea herds. Total complete genomes of four PCV2b isolates shared
nucleotide sequence homology each other, and were only
similar to one PCV2a isolate. ORF2 genome of four PCV2b isolates shared over 99% nucleotide sequence and deduced amino acid sequence identity to each other. Nevertheless, those were much divergent with the PCV2a isolate of this study and ranged from
nucleotide homology and
deduced amino acid sequence homology, respectively. The amino acid sequence alignments of the putative capsid protein identified three major regions of amino acid heterogeneity at residues
. Two of those correspond with dominant immunoreactive areas. Phylogenetic analysis based on the complete genome of PCV2 isolates showed that four PCV2b isolates of this study existed the closest relationship with European strains (Netherland, UK and France). One PCV2a isolate was closely related to Japan and North America strains.
Genetic sequence analysis of Porcine epidemic diarrhea virus (PEDV) detected from postweaning pigs in Korea
Shin, Hyun-Geun ; Kim, Yeong-Hun ; Seo, Tae-Won ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 11~18
Porcine epidemic diarrhea virus (PEDV), an enveloped single stranded RNA virus in the family Coronaviridae, causes acute viral enteric disease in piglets. Recently outbreaks of porcine epidemic diarrhea (PED) have been rare in Europe but frequent in Asia. In Korea, the increase of PED prevalence is showing specially in postweaning pigs. The purpose of this study was to investigate nucleotide sequence of nucleocapsid protein gene of PEDV field isolates from postweaning pigs in Korea and get more information about the viruses. A total of 15 postweaing pigs clinically suspected of PEDV infection by severe watery diarrhea and dehydration were used in this study. Viral RNA was extracted from small intestines and stools of the pigs. The N gene was amplified by nested RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. Three PEDVs were isolated from the suspected postweaning pigs. The N gene of three PEDV field isolates consisted of 483 nucleotides. These PEDV field isolates showed nucleotide sequence homology range from 99.6% to 95% with Chinese strains, from 99.8% to 95.2% with Korean strains, from 97.3% to 95.7% with Japanese strains and from 96.5% to 95.7% with Belgium and British strains. The encoded pritein shared range from 98.8% to 95.6% with Chinese strains, from 99.4% to 95% with Korean strains, from 97.5% to 96.3% with Japanese strains, from 95.6% to 95% with Belgium and British strains. By phylogenetic tree analysis based on nucleotide sequence, three PEDV field isolates were clustered into two groups which were Chinese isolate groups and other Korean isolate groups. These results indicated that some of PEDV field isolates prevailing in Korean postweaning pigs may be associated with those of Chinese strains and other Korean strains.
Expression of ORF6 gene of porcine reproductive and respiratory syndrome (PRRS) virus
Bae, Su-Jung ; Kim, Jin-Won ; Yoon, Young-Sim ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 19~25
Porcine reproductive and respiratory syndrome (PRRS) virus is the etiological agent of diseases characterized by reproductive losses in sows and respiratory disorders in piglets. The PRRS virus is a small enveloped virus containing a positive-sense, single-stranded RNA genome. In the present study, ORF6 gene of Korean PRRS virus isolate, CNV, was cloned and expressed in baculovirus expression system. The ORF6 gene and expressed protein in the recombinant virus were confirmed by PCR/indirect fluorescence antibody (IFA) test and Western blotting, respectively. The recombinant protein with a molecular weight of approximately 24KDa was confirmed by Western blotting using His6 and PRRS virus-specific antiserum. Expressed ORF6 protein was applied for IFA to detect antibody against PRRS virus using field porcine sera. However, the sensitivity and specificity of developed IFA using expressed ORF6 protein were considerably low compared to those of commercial ELISA kit. This results suggest that IFA using expressed ORF6 protein could not be used as a diagnostic test for PRRS virus infection without further improvements.
Seroprevalence of selected porcine respiratory pathogens in the pig herds in Chungcheong and Gyeongsang provinces in Korea
Chu, Jia-Qi ; Hu, Xu-Min ; Kim, Myung-Cheol ; Park, Chang-Sik ; Jun, Moo-Hyung ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 27~32
We studied the seroprevalence of four respiratory pathogens in Korean swine farms located in Chungnam, Chungbuk, Gyeongnam and Gyeongbuk provinces during the period of spring of 2007 to winter of 2008. Serological tests were performed using commercial ELISA kits. A total of 530 serum samples were tested for the antibodies against porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), Mycoplasma hyopneumoniae (M. hyo) and Actinobacillus pleuropneumoniae (APP). Seroprevalence for four respiratory pathogens were estimated by ELISA-positive rates of the submitted samples. The overall seropositive rates of PRRSV, APP, M. hyo and PCV2 were 32.6%, 10.6%, 38.4% and 88.5%, respectively. By production stage, the seropositive rate for PRRSV was highest in nursery pig populations (46.2%). In contrast, the highest seropositive rates of APP and M. hyo were observed in sow and growing pigs. However, the seroprevalence of PCV2 was ranged from 85.7% to 89.6%, showing no significant difference among the production stages. In the seroprevalence by season, PRRSV, APP and M. hyo infections revealed typical seasonal patterns that the peaks of the seropositive rates were observed between early winter and late spring. In case of PCV2, no particular seasonal patterns were noticed. The pig herds in Gyeongbuk province where PMWS was endemic during the period of survey showed the highest seropositive rates for PRRSV (44.6%), M. hyo (47.5%), and PCV2 (92.7%). Seropositive rates for APP of four provinces were approximately 10%. These results might be valuable for control and prevention of the respiratory diseases and helpful to define strategies related to vaccine applications.
In vitro selection of lactic acid bacteria for probiotic use in pig
Ryu, Ji-Sook ; Han, Sun-Kyung ; Shin, Myeong-Soo ; Lee, Wan-Kyu ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 33~41
In order to develop probiotic strain for pigs, Lactobacillus spp. (527 isolates), Streptococcus spp. (95 isolates) and Bifidobacterium spp. (25 isolates) were isolated from the feces of 35 pigs. These isolates were tested through in vitro experiment such as acid tolerance at pH 2.0 (Lactobacillus spp. and Streptococcus spp.) or pH 3.0 (Bifidobacterium spp.), bile tolerance in MRS broth containing 0.3% (w/v) Oxgall, heat resistance at
for 5 min, antibiotic resistance, antimicrobial activity against pathogenic bacteria and Caco-2 cell adherence assay. Finally ten most superior strain (5 Lactobacillus spp. strain, 3 Bifidobacterium spp. strain and 2 Streptococcus spp. strain) were selected as potential candidate for probiotic use in pig industry. It could be used as an alternative to antibiotics in feed additives.
Biochemical characteristics, antimicrobial susceptibility of Salmonella Gallinarum detection in chickens and rapid diagnosis by polymerase chain reaction
Chu, Keurn-Suk ; Lee, Jeong-Won ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 43~48
Salmonella enterica serovar Gallinarum is the causative agent of fowl typhoid (FT) and Salmonella enterica serovar Pullorum is pullorum disease (PD), a severe systemic disease of chick and it has the same antigenic fomula, the close relation but distinct pathogen. The traditional bacteriologic and serologic methods routinely used but tedious, time consuming. some of biochemical differences are helpful in differentiating the two organisms, however variation in the characteristics of some strains can be observed. During 2006 to 2008, there was isolated 30 strains. The biochemical characteristics of S. Gallinarum was nonmotile, fermentation of dulcitol, maltose but positive arginine (6.6%), lysine (83.3%) and arabinose (20.0%). The antimicrobial susceptibility test showed 100% sensitive to amikacin, ampicillin, amoxicillin/clavulanic acid and florfenicol, but resistant to penicillin (100%) and erythromycin (60.0%). This PCR method can be applied in the diagnosis between S. Gallinarum and S. Pullorum.
Serotype and antimicrobial susceptibility of Salmonella spp. isolated from pigs and cattle
Lee, Woo-Won ; Jung, Byeong-Yeal ; Lee, Gang-Rok ; Lee, Dong-Soo ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 49~59
At the present study, it was aimed to explore the states of antimicrobial resistant Salmonella spp. isolates from 3,850 pigs (2,732 ileocecocolic lymphnodes and 1,118 cecal contents) and 1,764 cattle (965 cecal lymphnodes and 799 cecal contents) slaughtered in Busan province from December 2000 to November 2001. Among 5,614 samples, 457 of Salmonella spp. were isolated from pig lymphnodes (13.5%), pig cecal contents (4.4%), cattle lymphnodes (3.5%) and cattle cecal contents (0.5%). Salmonella spp. were showed different isolation ratio, that was 10.8% in summer, 9.0% in autumn, 8.4% in spring and 5.0% in winter. As a result of serotyping, B group (65.4%) were identified as the most common in pigs and cattle, in order of
(4.2%) and L (3.5%). 34 serotypes were found, among them, Salmonella Typhimurium (S. Typhimurium) (21.0%) was the most common serotype from pigs and cattle. The major serotypes were in order of S. Derby (15.3%), S. Schwarzengrund (14.7%), S. Typhimurium var Copenhagen (9.2%), S. Mbandaka (5.7%), S. Enteritidis (5.5%) and S. Ruiru (3.5%). The most common serotype was S. Typhimurium in pigs, and S. Ruiru in cattle. S. Ruiru was firstly isolated from pigs and cattle in Korea. In antimicrobial susceptibility test, all the isolates were demonstrated susceptibility to norfloxacin and ofloxacin. But the isolates were showed resistance other antibiotics in order of doxycycline (68.3%), tetracycline (67.8%), penicillin (54.5%) and streptomycin (52.5%). S. Typhimurium were exhibited resistance to ampicillin (34.8%), chloramphenicol (36.2%), streptomycin (94.9%), sulfamethoxazole/trimethoprim (34.8%) and tetracycline (97.8%). There were 53 strains (38.4%) which had multi drug resistant (MDR) isolates, resistant to more than 6 antimicrobial agents. The most common resistance patterns of MDR isolates were ampicillin, chloramphenicol, carbenicillin, doxycycline, nalidixic acid, penicillin, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACCbDNaPSSuT).
Molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolated from pigs and cattle
Lee, Woo-Won ; Jung, Byeong-Yeal ; Lee, Gang-Rok ; Lee, Dong-Soo ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 61~76
At the present study, it was aimed to explore the molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolates from pigs and cattle. A total of 138 Salmonella Typhimurium (S. Typhimurium) isolates were typed with phage, among them, 83.3% of S. Typhimurium tested could divide into a 10 phage types. Definitive type 193 (DT193) (25.4%) and DT195 (24.6%) were exhibited as the dominant types. DT104 and U302 were found from pigs and cattle. On the other hand, S. Enteritidis had 6 phage types, of them, phage type 21 (PT21) and PT11b were the popular types. In the plasmid profiles, 135 of S. Typhimurium isolates were exhibited 1 to 6 plasmid bands which molecular weight ranged from 90 to 2kb. 35 isolates (25.4%) harbored a 90kb plasmid which is thought to be the serotype specific virulence plasmid. Two of twenty five S. Enteritidis had common plasmids at 2 and 1.5kb. With multiplex polymerase chain reaction, virulence genes (invA and spvC) were detected from all Salmonella spp. from 167 of S. Typhimurium, S. Enteritidis and chloramphenicol resistant S. Schwarzengrund, but some drug resistant genes, such as PSE-1, cml/tetR and flo were not determined but other drug resistant genes, for example TEM and int were found. The detection rates of spvC, TEM and int gene was 35.3%, 29.3% and 72.5%, respectively. The TEM gene was highly popular in S. Typhimurium, which was detected from ampicillin and amoxicillin resistant strains as 95.9%. int gene was able to detect from all the isolates identified as multidrug resistsnt (MDR), particularly DT193 was thought as the most prevalent virulence and multidrug resistance isolate. The major plasmid profile and drug resistance pattern of DT193 were 90, 40, 10.5, 6.3, 3.0kb and ACCbDNaPSSuT, respectively. MDR was commonly found in other phage types, particularly DT104, U302 and DT203.
Effects of organic acids on prevention against S. Typhimurium in weaning pigs
Yoon, Hyun-Keun ; Kim, Yeong-Hun ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 77~82
Salmonella (S.) Typhimurium infection in pigs is a major source of human foodborne salmonellosis. Recently, the swine industry in Korea has been suffered from salmonellosis causing severe economic loss to farms. Organic acids have antibacterial activity which prevents bacteria from multiplying by reducing the pH in the gastrointestinal tract. The aim of the present study is to evaluate whether
mixed with organic acids is able to have influence on growth performance and whether it prevents against S. Typhimurium in weaning pigs. Four experimental treatments were examined: general diet added with 0.3% of
after S. Typhimurium (
) challenge, group A; commercial feed after Salmonella (
) challenge, group B; commercial feed and
, group C; commercial feed, Group D. At the end of the study, no significant differences in daily body weight gain and feed intake were observed between groups treated with
and groups treated without, whereas feed conversion ratio (FCR) tended to be improved in groups treated with
between days 1 to 14. Serological and microbiological evolution of the infection were examined by ELISA and microbiological culture from serums and fecal samples, respectively. In groups that challenged by S. Typhimuriums, the group without
showed higher prevalence and bacterial shedding compared to the groups treated with
. Our results suggest that the administration of
could be used to promote feed efficiency and to reduce the prevalence of salmonellosis in weaners.
Characteristics of bovine pulmonary parasites in Bangladesh
Rahman, A. K. M. Anisur ; Begum, Nurjahan ; Rahman, Md Siddiqur ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 83~85
In a cross-sectional abattoir survey of bovine pulmonary diseases in Mymensingh, during September 2001 to April 2002, subclinical Dictyocaulus viviparous infection was found in mesoendemic level in Bangladeshi adult zebu cattle. The number of mature lung worms varied from
in individual lungs. One to five mature lung worms were most frequent (74.2%) followed by 6 to 10(19.3%) and
(6.5%). Of the total 123 mature worms collected from 31 lungs, there were 50 (40.7%) male and 73 (59.3%) female worms. The female lungworms (mean length 14.41mm) were significantly (P<0.01) larger than the male lungworms (mean length 11.28mm). The number of hydatid cysts varied from
in individual lungs. One to five (
) cysts were recorded most commonly (76.0%) followed by
(4%). The size (diameter) of the hydatid cysts ranged from
. Of the total 203 hydatid cysts collected from 31 lungs 45 (22.2%) cysts were fertile and 158 (77.8%) cyst were sterile.
Repellent effect of essential oils from coniferous trees against the house dust mites (Dermatophagoides farinae and D. pteronyssinus)
Hong, Mo-Se ; Jee, Cha-Ho ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 87~92
The avoidance of the allergen of the house dust mite is one of the challenges to reduce and treat the symptoms of allergic diseases. Accordingly, various acaricidal agents are being used to kill the mite, but just killing it leaves the remains of it, which still act as the allergen. Therefore expelling the mite is thought as best policy to avoid the mite allergen. For this, some materials have been applied to repellent agents against the mite. Among them, a material with natural origin, known as the phytoncide, is being used for its repellent activity, as well as for its benefits for health. In this experiment, essential oils extracted from Korean white pine (Pinus koreaiensis S. et Z.) and hinoki cypress (Chamaecyparis obtusa), which are widely used as the source of phytoncide products, are studied for demonstration of the repellent effect against the house dust mites, Dermatophagoides farinae and D. pteronyssinus. Direct contact method was used to evaluate the repellent effect (%). And the results suggest the oils have a significant effect enough to be used as a source of repellent agent. For the repellent effect, the most effective concentration was
at 45 minutes both in the white pine and the hinoki oil.
Study on antimicrobial resistance of Escherichia coli isolated from domestic beef on sale (2)
Kim, Hong-Tae ; Jung, Kyung-Tae ; Lee, Dong-Soo ; Lee, Keun-Woo ;
Korean Journal of Veterinary Service, volume 32, issue 1, 2009, Pages 93~102
In this study, antimicrobial resistance of E. coli isolated from domestic beef on sale in Busan and Gyeongnam province was investigated from March to October 2008. A total of 400 beef samples were collected for the monitoring of antimicrobial resistance, and 39 (9.8%) strains of E. coli were isolated. Antimicrobial resistance test was carried out by agar disc diffusion method with 17 antimicrobials. In general, E. coli isolates showed the highest antimicrobial resistance to tetracycline (85.3%), followed by doxycycline (76.5%), streptomycin (61.8%) and sulfamethoxazole/trimethoprim (61.8%). Then they showed higher resistance to several antimicrobials like kanamycin and neomycin (55.9%). However, They had low antimicrobial resistance to amikacin (8.8%), amoxicillin/clavulanic acid (2.9%). Of 39 isolates, 31 (79.5%) were resistant to more than 2 antimicrobials. Among 17 antimicrobials examined, tetracyclines were the most resistant, followed by aminoglycosides, sulfonamides. The resistance was seemed to be correlated to amounts of antimicrobial use. In the result of this study, we suggest that there be need to regulate the abuse of antimicrobial on food-producing animals in Korea because the concern on antimicrobial resistant is gradually increased worldwide.