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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 32, Issue 4 - Dec 2009
Volume 32, Issue 3 - Sep 2009
Volume 32, Issue 2 - Jun 2009
Volume 32, Issue 1 - Mar 2009
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Detection of influenza A viruses by RT-PCR with single primer of nonstructural gene
Moon, Hyeong-Sun ; Bae, Yoon-Yeong ; Jin, Ji-Dong ; Kang, Zheng-Wu ; Hahn, Tae-Wook ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 103~109
Influeza type A virus have been worldwide problematic in animals as well as in humans. In this study, the use of reverse-transcriptase polymerase chain reaction (RT-PCR) was described for detecting influenza virus type A. The primer of RT-PCR was designed from an nonstructural (NS) gene of Influenza A virus. By RT-PCR, a product with the size of 189 bp was detected only when influenza virus type A was used as template. No products could be detected with Influenza virus type B as well as other respiratory pathogens. The detection limit of the RT-PCR was up to
which is comparable to the sensitivity of cell culture method. The RT-PCR could detect the influenza A virus from nasal turbinates of the ferrets infected with influenza virus type A not type B.
Prevalence of antibodies against bovine viral infectious diseases in farmed deer and wild water deer in Jeonbuk province
Jo, Young-Suk ; Chu, Keum-Suk ; Lee, Jeong-Won ; Camer, Gerry A ; Chekarova, Irina ; Seol, Min-Suk ; Park, Hyun-Jong ; Kim, Bum-Seok ; Lim, Chae-Woong ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 111~117
Farmed deer could be susceptible carrier to bovine viral infectious disease. But unfortunately, there has not been an overall study over this subject in Korea so far. Therefore, a study was conducted to see serum antibodies to bovine leukosis, food and mouth disease, bovine viral diarrhea and infectious bovine rhinotracheitis in deer using the sera of farmed deer. As a result, two deer in a farms showed positive in bovine leukosis antibodies, using ELISA. For wild water deer, no antibodies were found for those diseases. As a result, it can be assumed that deer were relatively low rate of exposure to highly contagious disease such as viral bovine infectious disease in Korea. As this study was conducted over limited in number of subject and regions, continued study should be carried out in order to prevent and control the interspecies transmission in the future.
Epidemiological studies on bovine tuberculosis in mass outbreak region
Cho, Bum-Jun ; Chu, Keum-Suk ; Cho, Young-Suk ; Kang, Mi-Seon ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 119~124
The epidemiological survey of mass outbreak region of bovine tuberculosis from January of 2007 through May in 2009. The results were enumerated as follows. The results of tuberculin skin test are: 7 (0.4%) out of 1,697 in 2007, 61 (2.8%) out of 2,163 in 2008, 80 (4.9%) out of 1,639 in 2009. The sex and age distribution among the incidence of positive: 135 (91.8%) out of 147 in female, 12 (8.2%) in male. Among female, age 1: 6.1%, age 2: 30.6%, 3: 38.8%, 4: 14.2%, 5: 0.7% and 6: 1.4%. Among male, age 1: 4.1%, 2: 1.4%, 3: 2.7% and more frequent occurrence in age 3, 38.8% in female and 2.7% in male. The rate of recurrence by farms: recurrence 1: 6 (35.3%), 2: 9 (52.9%), 3: 1 (5.9%), 6: 1 (5.9%), The recurrence rate of 2 or more was 64.7%. The ELISA test result among 114 heads over 14 farms: 75 (65.8%) showed positive and 39 (34.2%) negative. Geographical distribution of recurrence is characterized as concentrated along the major traffic and stream crossing the village, and spread from the high elevation to downward area.
Studies on the diagnosis of purified protein derivatives (PPD) tuberculin intradermal tuberculin test and ELISA to antibodies of Mycobacterium bovis
Chu, Keum-Suk ; Cho, Bum-Jun ; Cho, Young-Suk ; Kang, Mi-Seon ; Oh, Jin-Sik ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 125~129
SBovine tuberculosis is a chronic bacterial disease of animals and humans caused by Mycobacterium bovis. Besides the classical intradermal tuberculin test, a number of blood and serum tests have been used. The purpose of this study was to establish seroprevalence of M. bovis. The sera were screened using the ELISA technique. A total seroprevalence of 65.8% in positive cattle, suspect 36.0%, negative 5.9% in TB-infected herds by PPD and dairy cattle is 3.0%, Hanwoo is 1.6% in TB-free herds. The deer of seroprevalence is 55.0% in TB-infected herd and 7.7% in TB-free herds.
Prevalence of antibodies to Toxoplasma gondii in cattle and pigs reared in eastern areas of Gyeongbuk province
Seo, Min-Goo ; Jang, Young-Sul ; Lee, Eun-Mi ; Park, No-Chan ; Kwak, Dong-Mi ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 131~137
This study was conducted to determine the prevalence of antibodies to Toxoplasma gondii (TG) in cattle and pigs reared in eastern areas of Gyeongbuk province by ELISA. Among 368 sera collected from 119 cattle farms, 76 (20.7%) sera from 34 (28.6%) farms had antibodies to TG. Fifty (27.2%) out of 184 cattle in Uljin-gun and 26 (14.1%) out of 184 cattle in Yeongdeok-gun were positive. Pyeonghae (50.0%) in Uljin-gun and Dalsan (33.3%) in Yeongdeok-gun had the highest TG antibodies in cattle compared to other areas. Prevalence of TG antibodies in cattle was increased with age. Among 368 sera collected from 43 pig farms, 62 (16.8%) sera from 16 (37.2%) farms had antibodies to TG. Forty (21.7%) out of 184 pigs in Uljin-gun and 22 (12.0%) out of 184 pigs in Yeongdeok-gun were positive. Uljin and Puk (40.0%) in Uljin-gun and Yeonghae (33.3%) in Yeongdeok-gun had the highest TG antibodies in pigs compared to other areas. Prevalence of TG antibodies in sows was higher than that in fattening pigs. Seasonally, prevalence of TG antibodies in pigs was highest in summer (23.4%) and lowest in winter (12.5%). Based on these observations, data indicate that infection by the protozoan parasite TG is widely prevalent in cattle and pigs reared in eastern areas of Gyeongbuk province.
Biochemical characteristics of Brucella abortus isolated from cattle in Gyungbuk province
Kim, Seong-Guk ; Kim, Young-Hoan ; Cho, Min-Hee ; Lee, Young-Ju ; Park, Cheong-Kyu ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 139~146
Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H
S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.
Identification of Salmonella Enteritidis and S. Typhimurium by multiplex polymerase chain reaction
Lee, Woo-Won ; Lee, Seung-Mi ; Lee, Gang-Rok ; Lee, Dong-Soo ; Park, Ho-Kuk ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 147~153
Salmonella species are the most important etiologic agents of food-borne acute gastroenteritis. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the polymerase chain reaction (PCR) has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a multiplex PCR (m-PCR) was used to detect S. Typhimurium and S. Enteritidis. We selected m-PCR target genes, which were the spv (virulence plasmid specific for S. Enteritidis) and sefA (S. Enteritidis fimbrial antigen) genes, fliC (H1-i antigen specific for S. Typhimurium) and a randomly cloned sequence specific for the genus Salmonella. With m-PCR, random sequence was detected from all strains of Salmonella spp, spv and sefA were detected from all strains of S. Enteritidis (100%), and fliC was detected from all strains of S. Typhimurium (100%). This assay indicate that the specificity of the m-PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.
Antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of Salmonella Gallinarum isolated from chicken
Bae, Jong-Chul ; Kim, Seong-Guk ; Kim, Young-Hoan ; Jo, Min-Hee ; Lee, Young-Ju ; Park, Cheong-Kyu ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 155~163
Fowl typhoid (FT) is a septicemic disease caused by Salmonella Gallinarum. The purpose of this study was to investigate the antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of S. Gallinarum isolated from chicken. During 1999 to 2004, there was isolated a total of 100 strains in liver and spleen. The biochemical characteristics of S. Gallinarum isolates was nonmotile, no production of H
S, glucose gas, non-fermented rhamnose, indole-negative, fermentation of dulcitol, mannitol, maltose, and ornithine decarboxylase. At antimicrobial susceptibility, all of isolates were susceptible to amoxicillin/clavulanic acid, amikacin, neomycin, kanamycin, norfloxacin and enrofloxacin. One hundred isolates were divided into 54 resistant patterns and 37 strains was 6-multi drug resistance. PFGE of Xba I restriction fragments of S. Gallinarum isolates was 20 patterns.
Coincident finding of bronchopneumonia by Pasteurella multocida, Bordetella bronchiseptica and Klebsiellosis in muskrat
Park, Mi-Young ; Jeon, Oh-Sook ; Cho, Yun-Kyoung ; Choi, Kyung-Mook ; Woo, Jong-Tae ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 165~169
Suppuratives bronchopneumonia was found in a 3-month old domestic muskrat (Ondatra zibethicus). Dead muskrat showed hemorrhagic nasal discharge, severe hemorrhage and consolidation were observed in the lungs in necropsy. Histologically, severe polymorphic neutrophils and alveolar macrophages were infiltrated in the bronchus, bronchioles, alveoli. P. multocida and B. bronchiseptica were identified from the lungs, Klebsiella was isolated from the cecum. We demonstrated those organisms by biochemical test and confirmed P. multocida capsular type A by means of polymerase chain reaction (PCR).
Seroprevalence of paratuberculosis of dairy cattle and Korean cattle in Eastern-Gyeongbuk area
Lee, Seon-Mi ; Kim, Mee-Sug ; Jang, Young-Sul ; Chon, Ryoung-Hoon ; Park, No-Chan ;
Korean Journal of Veterinary Service, volume 32, issue 2, 2009, Pages 171~176
Johne's disease is a chronic inflammatory bowel disease of cattle, sheep, goats and other ruminants, and Mycobacterium paratuberculosis is the etiologic agent of this disease. Many studies have been carried out on paratuberculosis from daily cattle and Korean native cattle in multiple areas around nation, but there is no report in Eastern-Gyeongbuk area. The purpose of this study is to investigate the seroprevalence of bovine paratuberculosis in Eastern-Gyeongbuk area. From July to December in 2007, blood samples were collected from 363 dairy cattle of 27 farms and 281 Korean cattle of 114 farms and the ELISA was conducted. 25 (6.9%) dairy cattle of 6 (22%) farms and 19 (6.8%) Korean cattle of 8 (7.0%) farms were positive in ELISA. In regional analysis, 25 (8.3%) out of 300 dairy cattle in Gyeungju were positive and Pohang were negative in this research. 12 (16.4%) out of 73 Korean cattle in Gyeungju and 7 (9.6%) out of 73 Korean cattle in Uljin were positive. Pohang and Youngdeok of Korean cattle were negative in this research. According to raising scale of dairy cattle, 4 (66.7%) farms out of 6 farms were raising 30 below and 2 (33.3%) farms out of were raising 30
70. And there were negative raising scale more than 70. In Korean cattle, 6 (75%) farms out of 8 were raising below 10 and 2 (25%) farms were raising 10
30. And there were negative raising scale more than 30. The rate of seropositive of paratuberculosis dairy cattle and Korean cattle were similar and the positive rate of Eastern-Gyeongbuk area is reported lower than that of any other region.