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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
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Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 33, Issue 4 - Dec 2010
Volume 33, Issue 3 - Sep 2010
Volume 33, Issue 2 - Jun 2010
Volume 33, Issue 1 - Mar 2010
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Monoclonal antibody-based enzyme immuno-slide assay (EISA) in the rapid diagnosis of Peste des petits ruminants of goats
Das, Kamol K. ; Rahman, M.B. ; Shil, N.K. ; Rahman, Md Siddiqur ; Jang, Hyung-Kwan ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 1~6
Monoclonal antibody (mAb)-based enzyme immune-slide assay (EISA) was used for the detection of Peste des petits ruminants (PPR) virus from field samples collected from a natural outbreak. The clinicopathological study was undertaken to diagnose the case primarily of PPR. Antigen was detected from discharges and faeces of infected goats and swabs of postmortem lesions prepared on glass slide or glass plate using acetone fixation. Nasal discharge collected at the early stage of disease course or lung is an appropriate ante- or postmortem sample for this technique, respectively. Convalescent polyclonal sera collected from recovered animals which were diagnosed as PPR by EISA showed high antibody titer against PPR by C-ELISA, demonstrating the satisfactory specificity of the test. Therefore, EISA is a sensitive and specific assay to confirm PPR infection both in field and laboratory conditions and especially suitable for developing country.
Coinfected cases with adenovirus, chicken infectious anemia virus and Newcastle disease in broiler chickens
Chu, Keum-Suk ; Kang, Mi-Seon ; Rim, Sang-Hyun ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 7~12
There are several immunosuppressive viral diseases in chickens such as avian adenovirus (AAV), chicken anemia virus (CAV), infectious bursal disease (IBD) and Marek's disease (MD). In this study, we have investigated two broiler chicken farms suffered from high mortality in Jeonbuk in July to August 2009. Clinically high fever and growth retardation were observed in the diseased chicken. In necropsy, the hemorrhages in thigh leg and thymus, hemorrhages and enlargement of liver, kidney and proventriculus, and yellowish fluid in heart were seen. Histologically, necrotic foci and basophilic intranuclear inclusion bodies of hepatocytes, hemorrhages and infiltrated lymphocytes in kidney and proventriculus were observed. By using polymerase chain reaction (PCR), the genes of avian adenovirus, CAV and ND virus were detected in specimens. We suggested that these coinfection cases with high mortality were due to primarily infection of immunosuppressive diseases such as avian adenovirus, CAV, followed by secondary infection of Newcastle disease (ND) virus.
Investigation of infection rate and genetic sequence analysis of chicken infectious anemia virus
Chu, Keum-Suk ; Kang, Mi-Seon ; Song, Hee-Jong ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 13~21
Chicken anemia virus (CAV) has been recognized as an immunosuppressive agent and plays role as an etiological agent of multifactorial diseases in chicken. In this study, we investigated distribution of CAV antibody by ELISA and the virus gene by PCR in poultry farms in Jeongeup, Jeonbuk province. In the test using ELISA kit, 41 (95.3%) of 43 flocks and 88.6% of the individual chickens were positive, respectively. By PCR, 90.9% of the broiler breeders and 75.0% of White-semi breeders were found positive, respectively. All hatchery was negative by PCR. Of the clinical cases from 49 poultry flocks, 87.5% of flocks and 54.7% for each samples were found positive by ELISA, respectively. By PCR test, 21 (42.9%) of 49 flocks were positive. Major clinical signs of the infected flocks were growth retardation, femoral subcutaneous bleeding, depression, limping, and continuing selection. The genetic analysis of separate N genes of CAV showed highly homologous each other. The nucleotide sequence of field isolates had homology ranged from 99.9% to 97.5% with Chinese strains, and 99.9% to 99.6% with Japanese strain. Phylogenetic analysis based on the N gene of CAV isolates showed the closely relation with Chinese strains. The results of this survey could be used as basic data for development of vaccine.
Investigation of Brucella canis infection in public animal shelters and kennels in Incheon
Hong, Ji-Young ; Park, Jin-Su ; Han, Tae-Ho ; Hwang, Hyun-Soon ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 23~27
This survey was performed to investigate the seroprevalence of antibodies to Brucella canis in dogs from public animal shelters and breeding kennels in Incheon. A total of 402 dogs selected randomly were tested serologically by using immunochromatographic antibody test kit. None of 289 dogs in public animal shelters were sero-positive and 10 (8.9%) of 113 dogs in breeding kennels were sero-positive. 5 (4.4%) strains of Brucella canis were isolated from 10 sero-positive dogs' blood. Antimicrobial susceptibility test was carried out by Disk diffusion method. They were susceptible to tetracyclines, quinolones, aminoglycosides and combination amoxicillin with clavulanic acid.
Efficacy of Brucella abortus strain RB51 vaccine in Korean mongrel dogs against virulent strains of B. abortus biotype 1 and B. canis
Hur, Jin ; Baek, Byeong-Kirl ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 29~35
This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with
CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis (
CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 (
CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.
Detection of Clostridium perfringens and its toxinotypes by enzyme linked immunosorbent assay from enterotoxaemic goats in Bangladesh
Islam, K.B.M.S. ; Rahman, M.S. ; Ershaduzzaman, Md. ; Taimur, M.J.F.A. ; Jang, Hyung-Kwan ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 37~44
An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.
Seroprevalence of Mycoplasma gallisepticum and M. synoviae in chickens
Jang, Seok-Hyun ; Kang, Zheng-Wu ; Jung, Chan-Eee ; Yoon, Jong-Ung ; Hahn, Tae-Wook ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 45~50
Mycoplasma gallisepticum (MG) is major cause of chronic respiratory disease in chickens. M. synoviae (MS) most frequently occurs a subclinical upper respiratory infection but may result in airsacculitis and synovitis in chickens and turkeys. Both mycoplasmas induce economic losses by triggering chronic respiratory signs, airsacculitis and decreased egg production. For prevention of the infections, live attenuated andinactivated vaccines are commercially used for prevention of MG but not MS in Korea. Serum plate agglutination (SPA) and enzyme-linked immunosorbent assay (ELISA) have been commonly used for serological diagnosis for MG and MS. Recently, it is believed that MS spread in chickens is very seriously in Korea and respiratory infection with MS causes substantial loss in poultry farms. In this study, we investigated the serological prevalence of MG and MS in unvaccinated chickens between 2008 and 2009. The overall seroprevalence of MG was 24% of 2,094 for individual chickens and 24% of 189 farms. The overall seroprevalence of MS was 36% in 2,095 chickens and 39% in 198 farms. The results show that seropositive ratio of MS is higher than MG. The geographical prevalence of MG has been estimated in following sequence; Gangwon, Jeolla, Gyeonggi, Gyeongsang, and Chungcheong. The geographical prevalence of MS has been estimated as follows; Gangwon, Gyeonggi, Gyeongsang, Chungcheong, and Jeolla. Seasonal seroprevalencewas also examined, and it found that seroprevalence in spring, fall and winter was higher than that in summer in MG, but not in MS. No significant difference was shown in seroprevalence according to breed. Future study about pathogenicity of MS isolates would be needed and economical losses by MS outbreaks should be analyzed. Moreover, we compared sero-positivity obtained with SPA and ELISA. The kappa value of MG between SPA and ELISA was 0.8061 and the kappa value of MS between SPA and ELISA was 0.7649.
Pattern of antimicrobial resistance and biochemical characteristics of Salmonella Typhimurium isolated from diseased pigs in Gyeongbuk province
Kim, Seong-Guk ; Eom, Hyun-Jung ; Kim, Soon-Tae ; Jang, Young-Sul ; Jo, Min-Hee ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 51~57
Salmonella Typhimurium is a virulent pathogen for human and animal. We studied serotypes, biochemical characteristics, and antimicrobial resistance of S. Typhimurium isolated from diseased pigs in Gyeongbuk province over 1998 to 2008. One hundred sixteen isolates were identified as S. Typhimurium by biochemical characteristics and serotypes from 90 farms. The biochemical characteristics of S. Typhimurium isolates was production of
, indole-negative, fermentation of mannitol, dulcitol, sorbitol, inositol, rhamnose, and maltose, and ornithine decarboxylase. At antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, cefepime, ciprofloxacin, while were highly resistant streptomycin, cephalothin, enrofloxacin, nalidixic acid, apramycin, chloramphenicol, tetracycline. The isolates were divided into 65 resistant patterns and 47 of the isolates were shown as a DT104 ASSSuT resistant phenotype.
Character of Listeria spp. isolated from livestock products and their related environmental areas
Hur, Jin ; Kim, Jun-Man ; Park, Young-Ho ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 59~66
This study was carried out to investigate the characters of Listeria monocytogenes isolated from food, animal feces, dry cattle food, and the environment in Seoul and Kyonggi province during the period from 1998 to 2003. Serotyping of 70 L. monocytogenes isolates was performed according to the manufacturer's instruction. Minimum inhibitory concentrations (MICs) were determined by the microdilution method according to the guidelines of the Clinical and Laboratory Standards Institute. All the isolates were tested against 20 antimicrobial agents. The serotypes of the 70 L. monocytogenes isolates were 1/2c (62.8%), 1/2a (20%) and 1/2b (17.2%). Of the 70 L. monocytogenes isolates, 67.1%, 57.1%, 11.4%, 5.7%, 2.8%, 1.4% and 1.4% were resistant to tetracycline (Te), minocycline (Mi), norfloxacin (Nor), ciprofloxacin (Cip), neomycin (N), chloramphenicol (C) and cephalothin (Cf), respectively. However, all isolates were 100% sensitive to antibiotics such as amikacin, ampicillin, erythromycin, gentamycin, imipenem, kanamycin, ofloxcin, streptomycin, penicillin, trimethoprim, trimethoprim/sulfamethoxazole, tobramycin, and vancomycin. Multiple resistance patterns of the isolates were observed in TeMiNor Cip (1.4%), TeMiNor (7.1%), TeMiCip (2.9%), TeMiN (1.4%) and TeMi (44.3%). The results of this study indicate that many L. monocytogenes isolates are resistant to antimicrobial agents including Te and Mi. The possibility that the isolates could increasingly acquire multiple antimicrobial resistant properties cannot be precluded.
Disease examination of slaughter pigs from Southern Gyeonggi-do
Woo, Jong-Tae ; Cheong, Yeon-Hee ; Kim, Min-Kyoung ; Ku, Kyung-Nyer ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 67~74
To reduce an economic loss by swine disease, this study was designed to provide helpful data which are particularly useful for each individual farm. From february to December 2009, a total of 18,173 slaughter pigs (612 herds) were randomly sampled, which were produced just in southern region of Gyeonggi-do. We examined all of them for the slaughter lesions of 8 kind diseases such as swine enzootic pneumonia (SEP), pleuritis (PL), pleuropneumonia (PP), white milk spot in liver (WMS), papular dermitis (PD), pericarditis (PC), ileitis (IL) and peritonitis (PT). Twenty four percentages (4431/18173 pigs) of the examined pigs had no lesions about 8 kind diseases. Among the pigs with lesions, the numbers of the pigs with just one lesion were 7,637(42%), followed by 4,551(25%) pigs with 2 lesions. Average prevalence of pigs were 56.5% (10288/18173 pigs) in SEP, followed by 34% in PL, 12.4% in PP, 10.1% in WMS, 6.1% in PD, 4.7% in PC, 0.1% in IL and PT, respectively. Each prevalence of SEP, PL, PP and PD was higher in spring than in winter, respectively (P<0.01). Among the pigs (n=6,105) with 2 or more than 2 kinds of lesions the top (55.5%) was the pigs with SEP and PL, and the second was 1,179 (19.3%). Swine enzootic pneumonia was considered as one of the more likely risk factors for initiation or/and acceleration of other diseases such as PL, PP, WMS and PD. The lesion of SEP was relatively severe since the pigs with late stage were more (7,277 pigs) than those with early stage.
A study on the prevalence of parasitic eggs in sands of playgrounds in public parks and public house in Incheon area (2004~2008)
Lee, Yun-Mi ; Kim, Kyoung-Ho ; Han, Tea-Ho ; Hwang, Hyun-Soon ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 75~80
Eggs of parasite were found 73 of 1,459 in sand samples from public parks and playgrounds in Incheon for 5 years (from 2004 to 2008). Of the 1,459 sand samples examined, 17.8% of samples were found to be positive ova of Toxocara canis, 11.0% for Toxocaris leonina, 5.5% for Toxocara cati, 2.7% Ancylostoma caninum and 1.4% for Strongyloides spp. It was found mixed infestation with Toxocara canis and Toxocara cati 60.2%.
A study of reduction effects of stillbirth and mortality rates in pigs by medicinal herbs supplementation in sow
Kim, Sang-Uk ; No, Jin-Gu ; Jo, Sang-Sin ; Jung, Ji-Yeong ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 81~87
This study was based on a survey of 100 sows which were raising in Jeonnam province for 11 months, from February to December 2008 in order to evaluate the medicinal herbs supplementation in sows on the effects of mortality in suckling pigs, and milk quality of sows. The medicinal herbs were supplemented in the feed of sows from 1 weeks before the expected date of delivery to 10 days after the delivery. As comparing stillbirth rate, test group was reduced 9.05%, and control group, 17.37%. It was certain that the medicinal herbs was very effective for reducing stillbirth rate. In comparison of mortality rate of suckling pig, test group was decreased by 1.52% as compared with control group. In analysis of milk components of sows, milk fat was 6.04% in test group and 5.22% in control group. Milk protein contents of test group and control group were 18.06% and 18.36%, respectively, showing no significant differences between two groups. Milk sugar contents showed also no difference between two groups. By milk somatic cell counts, test group (
cells/ml) was found decreased number of milk somatic cell as compared with control group (
cells/ml). These results imply that the medicinal herbs supplementation in sow might be effective for preventing stillbirth and mortality in piglets and mastitis in sow.
Hematological analyses of Japanese macaque (Macaca fuscata) housed in Everland Zoological Gardens
Kown, Soo-Whan ; Oh, Suk-Hun ; Shin, Nam-Shik ; Song, Hee-Jong ; Kwak, Dong-Mi ; Kwon, Oh-Deog ;
Korean Journal of Veterinary Service, volume 33, issue 1, 2010, Pages 89~96
Hematological and serum biochemical values were assessed from 20 clinically healthy Japanese Macaques raised in Everland Zoological Gardens and compared to the International Species Information System (ISIS) reference range that is used internationally as standard for wildlife animals. Taking our standard on sexual maturation at age 4, tRBC values in Macaques under age 4 were significantly lower than those over age 4, but the Hb and PCV values were significantly higher. Compared to the ISIS standard, the tRBC values in Macaques under age 4 were significantly lower whereas the Hb and MCHC values were significantly higher. Moreover, in the samples of Macaques over age 4, the PCV and MCV values were significantly lower than the ISIS standard. On serum biochemistry values the creatinine and amylase values in the Macaques under age 4 were significantly lower than those over age 4. In comparison with the ISIS standard, the values of ALT, ALP, BUN, IP,
in the Macaques under age 4 did have no significant difference. The values of TP, GGT, tBil, amylase, TG and UA were significantly higher than the ISIS standard, but the values of albumin, AST, glucose, creatinine, cholesterol, CPK, LDH,
and Clwere significantly lower. In contrast, the values of TP, albumin, ALT, ALP, creatinine, cholesterol, amylase, TG, IP and
in the Macaques over age 4 did have no significant difference, but the values of GGT, BUN, tBil, UA and
were significantly higher, while the values of AST, glucose, CPK, LDH,
were significantly lower. On the other hand, there was no significant difference in hematological and serum biochemical values between the groups of male and female.