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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 33, Issue 4 - Dec 2010
Volume 33, Issue 3 - Sep 2010
Volume 33, Issue 2 - Jun 2010
Volume 33, Issue 1 - Mar 2010
Selecting the target year
Clinical sign and transmission of foot-and-mouth disease in deer, Review
Park, Jong-Hyeon ; Lee, Kwang-Nyeong ; Kim, Su-Mi ; Ko, Young-Joon ; Lee, Hyang-Sim ; Cho, In-Soo ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 97~103
Foot-and-mouth disease (FMD) commonly infects cloven-hoofed livestock animals such as cattle, pig, sheep, and goat and its clinical signs are well-known. Besides livestock, FMD can be transmitted among cloven-hoofed animals in the wild. FMD mostly affects livestock animals in farms, but, wild animals are likely to play a pivotal role in spreading the disease due to their way of free living. In the case of deer, the clinical signs of FMD vary widely from subclinical to severe infections. Thus, in some deer species, it may be hard to verify clinical signs of FMD. A deer may carry the virus up to 11 weeks after exposure, shedding the virus during the period. However, deer is not considered as a typical host for persistent infection like buffalo, cattle or sheep. In Korea, small-scale livestock farms which have less than 10 animals make up 63.6% of the entire livestock farms. Considering raising environment in deer farms, it is assumed that the risk of virus excretion and consequent transmission of FMD among deers is relatively lower than other cloven-hoofed animals. However, Sika deer and Elk which are typical deer species in Korea would manifest mild to subclinical symptoms upon FMD infection. Therefore, laboratory testing is necessary to confirm FMD in these animals because of difficulty in verifying clinical signs and the risk of virus shedding during inapparent infection.
Production and characterization of monoclonal antibodies against rabies virus
Lee, Seung-Chul ; Yoon, Young-Sim ; Song, Yun-Kyung ; Woo, Gye-Hyeong ; Jean, Young-Hwa ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 105~111
Rabies virus which belongs to the genus Lyssavirus of the family Rhabdoviridae is known as a highly neurotropic virus and causes fatal encephalitis accompanied by severe neurological symptoms in almost all mammals, including humans. In this study, monoclonal antibodies (MAbs) against rabies virus were produced, characterized and applications of MAbs as diagnostic reagents were assessed Spleen and inguinal lymph node cells from Balb/c mouse immunized with purified rabies virus were fused with SP2/O myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing rabies virus-specific MAbs were screened by an indirect fluorescent antibody test. A total of ten MAbs were produced against rabies virus. The protein specificity and neutralizing activity of MAbs were determined by Western blot analysis and fluorescent antibody virus neutralization test, respectively. As a result, two MAbs, 5G3 and 6H4 had specificity for nucleoprotein (N protein) and two other MAbs, 5B1 and 5C1 had neutralizing activity for rabies virus. Some MAbs recognized the rabies virus-infected bovine brain stem cells by immunohistochemistry (IHC) assay. In conclusion, it was confirmed that MAbs produced in this study were rabies virusspecific and could be used as reliable diagnostic reagents for the detection of rabies virus.
Indirect enzyme linked immunosorbent assay for the diagnosis of brucellosis in cattle
Rahman, Siddiqur ; Huque, Fazlul ; Ahasan, Shamim ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 113~119
Brucellosis is a major zoonosis caused by Gram negative facultative intracellular bacterial organisms of the genus Brucella that are pathogenic for a wide variety of animals and human beings. Because of its economic impact on animal health and the risk to the human population,most countries have a brucellosis control program. Brucellosis is also an economically important andprevalent disease in Bangladesh. The accurate and prompt diagnosis is very important in controlling and eradicating of the disease in animals. The present study was undertaken to determine the seroprevalence of brucellosis in cattle in Mymensingh and Patuakhali district of Bangladesh. A total of 120 serum samples were collected from the two districts along with a questionnaire related to the epidemiology of the disease. The sampleswere screened by using slow agglutination test and conformed by indirect enzyme linked immunosorbent assay. The overall seroprevalence of brucellosis in cattle was 5% and it was observed that, a higher prevalence of Brucella was found in female than male, through natural breeding than artificial insemination (AI) and animal above 4 years old are highly susceptible than younger ones. Higher prevalence was found in aborted animals in comparison with non aborted animal. Finally, the study revealed that the female animal has more susceptible to brucellosis and healthy semen should be used for AI.
A sero-surveillance of Brucella spp. antibodies and individual risk factors of infection in cattle in Bangladesh
Ahasan, Shamim ; Rahman, Siddiqur ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 121~128
Brucellosis is a serious zoonosis, recognized worldwide. It primarily affects animals, which act as reservoirs for human infection as well as being of economic significance to the agri-food industry. Bangladesh has been reported as an endemic area for brucellosis. So a cross sectional study was conducted to determine the seroprevalence and potential risk factors of brucellosis in cattle in Dinajpur and Mymensingh districts of Bangladesh. A total of 182 cattle were examined by Rose Bengal Plate Test (RBPT) between September 2008 and October 2009. Then Positive, doubtful, and negative samples were further confirmed with slow agglutination test (SAT) and both indirect and competitive enzyme linked immunosorbent assay (iELISA and cELISA). A questionnaire was used to collect epidemiological information of the animals. The overall animal-level prevalence was 3.30%. Brucellosis seroprevalence was higher (4.76% by cELISA) in cattle above 48 months than those under 48 months. Female showed higher seroprevalence (10.67%) than male (6.25%). Higher seroprevalence was also found in cattle bred naturally (20.0%) than artificially (8.77%) and cattle that aborted or with previous abortion record (22.22%) showed higher seroprevalence than non-aborted (7.69%). The sensitivity of RBT and SAT was found 100% as compared to cELISA standard test, whereas specificity of RBT (95.35%) was higher than that of SAT (94.32%).
Serological and bacteriological study on canine brucellosis in the large kennel farms in Gyeongbuk province
Kim, Seong-Guk ; Seo, Hee-Jin ; Kim, Soon-Tae ; Jang, Young-Sul ; Jo, Min-Hee ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 129~134
Canine brucellosis is a contagious disease of the reproductive tract that cause mainly abortion and infertility in dog. A serological and bacteriological survey was conducted for breeding kennels which were suffered from frequent outbreak of canine brucellosis in Gyeongbuk province in 2009. Among 138 samples, 45 serum samples were sero-positive. Brucella canis was isolated from 30 blood samples of the seropositive cases, and from 2 samples of 62 sero-negatives. The biochemical properties of 32 isolates were characterized with no production of H2S, no fermentation of carbohydrates, hydrolyzation of urea, and development of thionin dye medium. At amplification of BCSP and 16S-rRNA gene using PCR, 711bp and 905bp DNA fragments were detected in agarose. Three tandem repeat pattern was shown in genotyping by Multi-locus VNTR assay (MLVA).
Extraction and verification of highly immunologenic antigen for diagnosis of bovine brucellosis
Bae, Jae-Hyung ; Jo, Sang-Rae ; Jeong, Eun-Hui ; Jang, Eun-Hui ; Kim, Seong-Eun ; Kwon, Hui-Nyeong ; Park, Dong-Yeop ; Lee, Kuk-Cheon ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 135~141
Bovine brucellosis, an important zoonosis, is diagnosed with serological tests such as the RBT, TAT using inactivated whole bacterial cells or bacterial lipopolysaccharide (LPS) antigen in Korea. However, a strong cross-reaction between Brucella spp. and Yersinia enterocolitica O9 in these tests has seriously complicated the diagnosis of animal brucellosis because Brucella spp. shares common antigenic determinants with Y. enterocolitica O9 in the smooth LPS region. In this study, Brucella-field strains were isolated from Brucellapositive Hanwoo in Kimhae, Korea and outer membrane protein (omp) which has low cross-reaction with Y. enterocolitica O9 and high immunogenicity was extracted from the field strains Then we compared ELISA using the extract with RBT-TAT. Fifteen field strains were isolated from 47 supra-mammary-lymph nodes, which were collected from 18 farms. Isolation rate was 32%. Brucella-specific antigen was identified by performing SDS-PAGE or Western blotting on extracted omp with at 0.5% n-lauroylsarcosine One hundred and ninety-two serum-samples were used in the experiment: 142 negative and 50 positive samples verified by RBT-TAT. According to ELISA results, 127 samples were negative and 15 appeared positive among 142 negatives by RBT-TAT, while 42 samples were positive and 8 were negative among 50 positives by RBT-TAT. Therefore, it showed 89.4% of specificity and 84% of sensi-tivity. Through the current experiments, we could set up an ELISA based on the omp which has low cross-reaction and high immunogenicity and concluded that the omp could be a good material for accurate diagnosis of bovine brucellosis.
Prevalence and molecular characterization of tetracycline-resistant Enterococcus isolates from livestock
Kim, Chul-Min ; Kang, Su-Jin ; Lee, Beyong-Jong ; Lee, Sung-Jae ; Yuk, Dae-Su ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 143~149
BIn the present study, Enterococcus isolates originating from livestock were studied for the phenotypic and genotypic assessment of tetracycline resistance. A total of 74 isolates encompassing the species Enterococcus faecalis (n=12) and E. faecium (n=62) displayed phenotypic resistance to tetracycline. Tetracycline resistance gene [tet (M), 1,886bp] were sequenced by dye terminator cycle sequencing method and compared with tet (M) sequences available from the GenBank database. Sequencing analysis of PCR amplicons showed high homology to the reference strains ranging 97.2~100%. The tet (M) genes were divided into three major subgroups according to phylogenetic analysis. The genetic information obtained from this study could be useful for the molecular study of enterococci.
Seroprevalence of antibodies to Neospora caninum in Korean indigenous cattle in Gyeongnam central area
Park, Ae-Ra ; Hah, Dae-Sik ; Jo, Seong-Suk ; Kwun, Young-Taek ; Park, Dong-Yeop ; Lee, Kuk-Cheon ; Heo, Jung-Ho ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 151~156
This survey was carried out to investigate the seroprevalence of antibodies to Neospora caninum in Korean indigenous cattle that was raised in central area province Gyeongnam, Korea. A total of 719 sera were tested for N. caninum antibodies using ELISA (Herdcheck anti-Neospora, IDEXX Laboratories Inc., Westbrook, Maine USA). Seroprevalence of individual and farm were 29.8%(214/719) and 53.2%(50/94). Regional seropositive rates of the samples were 61%(47/77), 23.3%(7/30), 13.8%(49/355), 37.6%(77/205), 65.4%(34/52) at Changwon, Jinhae, Gimhae, Miryang, Yangsan, respectively. It showed difference at the age and on the herd size of farms. A herd of cattle above the age of 5 was more infective than under 4 years. And in seroprevalence by herd size farms having under 30 heads was top(35.7%). Seropositive 214 herds of N. caninum antibidies were tested for brucellosis by test tube. Positive rate of double infection was 16.4%(35/214).
Epidemiological survey on prevalence of Dirofilaria immitis infection in dogs of Ulsan area
Park, Chang-Eun ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 157~165
A survey of canine heartworm infections among 83 dogs (Male 42, Female 41) of pet dogs in Ulsan area was performed from March to July 2009 using antigen test. Of 83 dogs, 6 dogs (7.2%) were infected with the Dirofilaria immitis, and 5 dogs (6%) were detected to have microfilaria in the peripheral blood. The infection according to the age of 4~5, 6~7, 7 over years were 7.4%, 12.5%, and 10.5%, Also, A survey of canine heartworm infections among 23 dogs (Male 15, Female 8) of hunting dogs in Ulsan area was performed from July 2008 using antigen test. Of 23 dogs, 11 dogs (47.8%) were infected with the D. immitis, and 6 dogs (26.1%) were detected to have microfilaria in the peripheral blood. The infection according to the age of under 1, 2-3, 4-5, 6-7 over years were 12.5%, 40%, 85.7% and 66.7%, respectively.
Repellent effect of Eucalyptus essential oils against house dust mites (Dermatophagoides farinae and D. pteronyssinus)
Lee, Jae-Young ; Jee, Cha-Ho ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 167~171
Majority of asthma and atopic dermatitis are known to be spontaneously sensitive to house dust mite allergen. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. So, It is more effective method keeping the house dust mites away than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus is a diverse genus of flowering trees in the myrtle family, Myrtaceae. It has insect repellent properties and is an active ingredient in some commercial mosquito repellents. These studies were carried out to investigate repellent effects of Eucalyptus oil against house dust mites and compare lemongrass oil. Eucalyptus oil and lemongrass oil were exposed at different doses (0.2, 0.1, 0.05, 0.025, 0.0125,
) and different times (1, 3, 6 hours) on house dust mites. The most effective dose of Eucalyptus and lemongrass oils against house dust mites was
. Each repellent effect(%) in most effective dose was 90.3% (Eucalyptus) and 80.8% (lemongrass).
Application of chitosan resin formulae as a sustained-releasing form adjuvant
Kim, Sang-Uk ; Kang, Mun-Il ; Lee, Jae-Il ; Kim, Tae-Jung ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 173~176
Here, we report the suitability of using a resin-type chitosan formulae as a sustained-releasing form adjuvant in comparison with commercially well-known Freund's adjuvants. To induce the immunological responses, N-terminal region of Pasteurella multocida toxin was used as an antigen, which was found to be protective immunogen against P. multocida infection. Mice immunized with chitosan resin formulae showed statistically significant antibody induction (P<0.001) as much as that of Freund’s adjuvants. As a result, the resin-type sustained-releasing form chitosan formulae is thought to be a good candidate for a new type adjuvant.
Effects of honeybee (Apis mellifera L.) venom by water supply on the performance of broiler chicken
Han, Sang-Mi ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Oh, Baeg-Young ; Kim, Bong-Soon ; Lee, Woong ; Kim, Soon-Tae ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 177~183
This study was conducted to investigate the antibiotic effects of bee venom (Apis mellifera) on the growth performance and blood characteristics in broiler chicken. 1-day-old broiler chicks were randomly divided into 3 groups with 3 replicates of 5,000 birds each. The treatments were control without antibiotic and bee venom, 0.5ppm or 1ppm bee venom. The final body weight and body weight gain were significantly higher in bee venom than control (P<0.05). The feed conversion ratio in all treated groups were significantly improved as compared to that of control (P<0.05). No significant differences among the groups were observed in the contents of total cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein, albumin and globulin in blood serum. The white blood cell, red blood cell, heterophil, lymphocyte, and stress indicator (heterophil:lymphocyte rate) were not significantly different among the groups. The superoxide dismutase-like activities in the groups that were water containing bee venom were significantly increased compared to those in the control group (P<0.05). It has been suggested that bee venom promotes the growth of broilers without any negative effect when added in broiler water.
Alleviative effects of activated charcoal and Houttuynia cordata Thunb. in broiler chickens during aflatoxicosis
Hah, Dae-Sik ; Ji, Dae-Hae ; Jo, Sang-Rae ; Park, Ae-Ra ; Jung, Eun-Hee ; Park, Dong-Yeop ; Lee, Kuk-Cheon ; Heo, Jung-Ho ; Kim, Jong-Shu ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 185~197
This study was conducted to evaluate the alleviative effects of activated charcoal (AC) and Houttuynia cordata (HC) singly or in combination in broiler chickens during aflatoxicosis. Activated charcoal (1% or 0.5%) and H. cordata (1% or 0.5%) were mixed into the diets for the ability to reduced the deleterious effects of 2.4mg total aflatoxin
diet on growing broiler chickens from 1 to 21 days of age. A total of 160 1-day-old (Hyline Variety Brown) broiler chicks were housed in eight treatment groups [Control,
, AC 1%, HC 1%,
plus AC 1% plus HC 1%,
plus AC 1% plus HC 0.5%,
plus AC 0.5% plus HC 1%,
plus AC 0.5% plus HC 0.5%] each consisting of 20 chicks. Compared to control, 2.4mg
alone treatment group significantly decreased body weight gains of chickens. The addition of mixed AC 1% and HC 1% including 6, 7 groups to the 2.4mg
-containing diet moderately reduced the adverse effects of
on performances of chickens. The chickens consuming 2.4mg
plus AC 0.5% and HC 0.5%-containing diet showed very slightly reduced the adverse effects on investigated parameters compared to the
only treated group. Also, the single addition of AC or HC to the
-free diet had no adverse effects in chickens. These results suggest that AC and HC mixed can reduced the aflatoxicosis in broilers and may be contribute to a solution of the aflatoxicosis problem in poultry production.
Hypoglycemic effects of nano powder propolis on streptozotocin-induced diabetic rats
Chung, Nyun-Ki ; Cho, Young-Chae ; Ha, Chang-Su ; Kim, Hee-Sung ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 199~206
To evaluate the hypoglycemic effect of nano powder propolis, streptozotocin (STZ) induced diabetic rats were divided into 2 groups : diabetic control group and nano powder propolis (0.9ml) group. Then the rats were fed with nano-powder-propolis for 4 weeks. After 4 weeks, oral glucose tolerance test (oral GTT) was performed and blood sugar levels after 16 hours fasting, body weights, and blood lipid levels were measured. Finally, pancreas were collected and examined by histopathology and immunohistochemistry. In conclusion, the nano-powder-propolis was effective in the treatment of diabetes due to the reduction of blood sugar level and the regeneration of damaged
-cells observed in streptozotocin-induced diabetic rats.
Chromosome analysis by GTG banding technique in the DongGyeongi dogs
Choi, Seog-Kyu ; Sung, Ki-Chang ; Lee, Eun-Woo ; Park, Chang-Eun ;
Korean Journal of Veterinary Service, volume 33, issue 2, 2010, Pages 207~211
None of the numerous published canis idiogram and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. The karyotypes of DongGyeongi dog were analysed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with one another. There were no variations in karyotypes which were analysed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with sapsaree (or canis familiaris strains). It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation.