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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 33, Issue 4 - Dec 2010
Volume 33, Issue 3 - Sep 2010
Volume 33, Issue 2 - Jun 2010
Volume 33, Issue 1 - Mar 2010
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Prevalence on protective serum antibodies of canine distemper virus and canine parvovirus in Ulsan area
Sung, Ki-Chang ; Lee, Eun-Woo ; Park, Chang-Eun ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 213~221
The results from a total of 412 blood samples consisted of 187 samples from regular visiting group (RV), 94 samples from first visiting group (FV), 52 samples from abandoned group (A), 54 samples from special breeder group (SB), and 25 samples from preliminary breeder group (PB) showed that RV(94.7%) and SB(88.9%) groups had the higher levels of protective antibody, but PB (36.0%) group revealed the lowest level. Among 96 blood samples with lower protective antibody levels, 14 samples (14.6%), 72 samples (75.0%) and 10 samples (10.4%) were below the protective antibody levels to distemper/parvo-virus, distemper only and parvovirus only, respectively. These results implied that antibody to parvovirus was well generated than that to distemper. Eighty six samples (20.9%) showed the protective antibody titer under 1:96 to distemper and 24 samples (5.8%), the protective antibody titer under 1:40 to parvovirus.
Detection and genetic characterization of Lawsonia intracellularis from swine in Korea
Chu, Jia-Qi ; Hu, Xu-Min ; Kim, Myung-Cheol ; Park, Chang-Sik ; Jun, Moo-Hyung ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 223~231
A total of 191 samples collected from the commercial swine farms located in Chungnam province were investigated by PCR to estimate the prevalence of Lawsonia (L.) intracellularis infection. In the group of the pigs with proliferative enteritis, 14 (93.3%) of 15 intestinal samples and 12 (80.0%) of 15 feces were positive in PCR. In contrast, a relatively low positive rate (18.0%, 29 of 161 samples) was determined in the group of normal healthy pigs. The group of pigs over 120 days showed the highest positive rates (26.8%, 15 of 56 samples). In the comparison of the sequences of 210bp for species specific fragments and 301bp for outer membrane protein, the isolates (L1. L2) showed almost 100% identity with the reference L. intracellularis (L08049, USA). For the sequences of partial 16s rDNA, the homologies among the 5 isolates (L1-L5) were 97.4% to 99.3%, and those of 5 sequences (L1-L5) versus 5 overseas reference strains of L. intracellularis ranged from 98.6% to 99.8%. In the comparison of the nucleotide sequences among 5 isolates and other species in Desulfovibrionales showed 82.4 to 99.5% identities. The 5 isolates shared relatively low identities (76.9% to 84.4%) with the species of alpha-proteobacteria. In phylogenetic analysis based on the 16s rDNA sequences, all of the 5 isolates (L1-L5) were located in the same branch with the strains of L. intracellularis that were previously isolated from the pigs in USA and China. Seven strains of Desulfovibrio sp. were clustered in the neighboring branches, whereas alpha and gamma Proteobacteria showed distant relationship with L. intracellularis strains. The present findings suggest that L. intracellularis infection is endemic in the swine farms in the regions, and that the domestic isolates maintained very limited genetic variation.
Identification and staphylococcal cassette chromosome mec (SCCmec) type of methicillin-resistant coagulase-negative staphylococci isolated from chickens
Kong, Shin-Koog ; Yook, Sim-Yong ; Lee, Geon-Taek ; Kim, So-Yeon ; Hong, Young-Un ; Jung, Yoon-Taek ; Lee, Jung-Hwa ; Kim, Hee-Jeong ; Hwang, Soo-Myung ; Chang, Kyung-Soo ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 233~240
Methicillin-resistant coagulase-negative staphylococci (MRCNS) were isolated from the respiratory sites of chickens in 4 farms and slaughter house located in Chungnam provinces. Isolation of coagulase-negative staphylococci (CNS) was positive for 61 (26.6%) of the 229 chickens tested, and isolation of MRCNS was positive for 17 (27.9%) of the isolated CNS. A total of 17 MRCNS isolates were selected and subjected to identification. Of the 17 MRCNS isolates selected, 6 were identified as Staphylococcus cohnii, 2 as S. saprophyticus, 3 as S. simulans, 3 as S. lentus, 2 as S. carnosus, and 1 as S. xylosus. The MRCNS isolates were resistant to many beta-lactam antibiotics, and some isolates were also resistant to macrolide and aminoglycoside antibiotics. The mecA gene was detected in some isolates of each MRCNS strains. The mecA-positive isolates were classified into five staphylococcal cassette chromosome mec (SCCmec). SCCmec types I to IV were detected in isolates from chickens.
Surveillance of antimicobial resistance ratio of E. coli and Enterococcus spp. isolated from fecal and carcasses of pigs in slaughterhouse
Jeong, Kwi-Ok ; Heo, Jung-Ho ; Lee, Jong-Min ; Yun, I-Ran ; Choi, You-Jeong ; Kim, Jong-Shu ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 241~248
The present study was conducted to investigate isolation and antimicrobial resistance ratio of E. coli, E. faecium and E. faecalis from feces(l50 samples) and carcasses (150 samples) on slaughtered pigs from 6 slaughterhouse of 13 cities in the Gyeongnam during the period from January 2009 to December 2009. Isolation ratio of E. coli from feces and carcasses were 98 (65.3%) and 110(73.3%), respectively, and simultaneously, E. faecalis and E. faecium from feces and carcasses were isolated 21 (14%), 52(34.7%) and 18(12%), 14 (9.3%), respectively. All E. coli isolated from feces and carcasses except cefepime (0%) and ceftiofur (0%) were exhibited 2.4~83.6% of resistance to teteracycline (83.6%), ampicillin (68.2%), streptomycin (60%), chloram-phenicol (53.8%) and cephalothin (2.4%). All E. faecalis isolated from feces and carcasses except penicillin(0%) and vancomycin (0%) were exhibited 2.7~80.8% of resistance to teteracycline (80.8%), quinupristin/dalfopristin (78%), erythromycin (56.1%), streptomycin (43.8%) and bacitracin (2.7%). All E. faecium isolated from feces and carcasses except gentamicin (0%), vancomycin (0%), florfenicol (0%), linezloid (0%) and bacitracin (0%) were exhibited 3.1~53.1% of resistance to rifampin (53.1%), erythromycin and tetracycline (25%), penicillin (15.6%), ciprofloxacin (9.3%), and streptomycin, chloramphenicol, and quinupristin/dalfopristin (3.1%). According to the heard size, resistance ratio of E. coli strains isolated from feces and carcasses in slaughtered pigs-breeding farms over 1,500 heard to tetracycline, ampicillin, streptomycin and chloramphenicol showed higher resistance ratio (1.0~16.8%) than those of farms-breeding under 1,500 heard. From the our results, we suggest that a few of antimicrobials were used in the Gyeongnam than the other cities.
Serologic survey of the ruminant bacterial infectious diseases in farmed deer and wild water deer in Jeonbuk province
Jo, Young-Suk ; Chung, Yun-Shin ; So, Seung-Young ; Seol, Min-Suk ; Cho, Ho-Seong ; Kim, Bum-Seok ; Lim, Chae-Woong ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 249~254
Deer can be one of the susceptible animals to bovine infectious diseases, and thus, may play a role either as a reservoir or amplifier host for spreading the diseases to other species such as cattle and goat. This study was conducted to determine the serum antibodies to bacterial infectious diseases for brucellosis, tuberculosis (TB), paratuberculosis (Johne's disease) in deer. Serum samples were randomly collected from 78 deer from 31 farms at Jeonbuk province, and 7 wild water deer from Jeonbuk wild animal treatment center during 2005 to 2007, respectively. Four farm deer (5.1%) showed antibodies to tuberculosis using Antigen Rapid Bovine TB Ab Test Kit. One elk (1.3%) and one wild water deer had antibodies for paratuberculosis. Antibody against Brucellosis was not detected in tube agglutination test (TAT) and enzyme-linked immunosorbent assay (ELISA). These data suggest that caution should be applied to inspection of velvet, deer blood and meat for human consumption from deer because of zoonotic bacterial diseases in deer. In addition, farmed deer can be a transmissible host for zoonotic disease to diary or raising farm.
Isolation of Staphylococcus aureus and detection of enterotoxin from pigs and cattle carcass by PCR
Lee, Woo-Won ; Jung, Byeong-Yeal ; Kim, Sang-Hyun ; Lee, Seung-Mi ; Lee, Gang-Rok ; Kim, Geum-Hyang ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 255~261
At the present study, it was aimed to explore the states of antimicrobial resistant Staphylococcus aureus isolates from 320 pigs and cattle carcass (160 pigs and 160 cattle) slaughtered in Busan province from March 2008 to November 2009. Among 320 samples, 26 of Staphylococcus aureus were isolated from pigs (10.6%) and cattle (5.6%). In antimicrobial susceptibility test, all of the isolates were demonstrated susceptibility to oxacillin, cefoxitin, cephalothin, vancomycin, rifampin and linezolid. But the isolates were showed resistance other antibiotics in order of penicillin (92.3%), gentamicin (76.9%), tetracycline (69.2%), erythromycin (65.4%), and clindamycin (61.5%). In case of enterotoxin production, 7.7% of 2 strains produced enterotoxin A.
Antimicrobial susceptibility and detection of enterotoxin by multiplex PCR of Staphylococcus aureus isolated from dogs and cats in Seoul
Kim, Neung-Hee ; Chae, Hee-Sun ; Son, Hong-Rak ; Kim, Chang-Ki ; Kim, Sun-Heung ; Lee, Jung-Hark ; Kim, Chul-Hun ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 263~269
The antimicrobial susceptibility of 73 strains of Staphylococcus aureus isolated from dogs and cats was determined against 16 antimicrobial agents. All isolates were susceptible to cephalothin (CF), vancomycin (VA), rifampin (RA), quinupristin-dalfopristin (SYN) and linezolid (LZD), whereas more than 86.3% of the isolates were resistant to penicillin (P). In the case of enterotoxin production, 8.2% of 73 strains produced enterotoxin A and 1 strain produced enterotoxin C. Appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will need to be investigated in the future.
Serosurvey for antibodies against Neospora caninum in farmed deer and wild water deer in Jeonbuk province
Jo, Young-Suk ; Lee, Jeong-Won ; Chung, Yun-Shin ; So, Seung-Young ; Park, Hyun-Jong ; Cho, Ho-Seong ; Kim, Bum-Seok ; Lim, Chae-Woong ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 271~274
With deer farming now becoming recognized as one of the alternative livestock farming practices, there has been much attention on infectious diseases of cattle, but less awareness of these diseases in deer. Serum samples were randomly collected from 78 deer from 31 farms and 7 wild water deer from wild animal medical center during 2005 to 2007, respectively. A total 85 sera were tested for Neospora caninum antibodies by ELISA. Fourteen farmed deer(17.9%) were positive to N. caninum but no antibodies were found in sera from wild water deer. The sera from all of the area for these study showed positive in the range from 6.7 to 30.7%. On the basis of these study, farmed deer in Jeonbuk province were exposed extensively and seriously to N. caninum which might present a risk to other species included cattle and dog.
Nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice
Yun, I-Ran ; Choi, You-Jeong ; Heo, Jung-Ho ; Choi, Chul-Yung ; Seoung, Tae-Jong ; Kim, Yun-Geun ; Kim, Jong-Shu ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 275~285
This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of gal gun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.
Effects of honeybee (Apis mellifera L.) venom on the reproductive efficiency of dams and the growth performance, disease occurrence of Hanwoo calves
Han, Sang-Mi ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Oh, Baeg-Young ; Kim, Soon-Tae ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 287~292
This study was conducted to investigate effects of bee venom (Apis mellifera L.) on the calving, reproductive efficiency of Hanwoo dams and the growth performance and disease occurrence of calves. Eighty seven experimental dams were allocated into two groups, bee venom treated (n=53, BV) and non-treated (n=34, control) of Hanwoo dams in Icheon, Kyonggi province. Calving period (
) and percentages of retained placenta (7.5%) in BV was shortened compared to control (
, 14.7%, respectively. P>0.05). Post-placenta periods was similar between two groups (P>0.05). Postpartum days (
) to first estrus and calving interval (
) were shortened compared to control (
, respectively) but have not significantly affected (P>0.05). Body weights (
) and average daily gains (0.62) of calves before 30 days old in BV were significantly higher than control (
, 0.52, respectively). The occurrence of respiratory disease and diarrhea in BV was relatively lower compared with Control. The present results indicate that bee venom may affect reproductive efficiency of dams and growth performance of calves.
Investigation of serum protein electrophoresis fractions analysis in Gyeongju DongGyeong dogs
Park, Chang-Eun ; Lee, Eun-Woo ; Sung, Ki-Chang ; Choi, Seog-Gyu ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 293~297
Serum proteins of Korean native dogs (Gyeongju DongGyeong dogs) was analyzed by capillary electrophoresis system. Electrophoretic patterns showed six fractions. Total serum protein and ratio of albumin to globulin were
(g/dl) in DongGyeong dogs. Relative percentages of total serum albumin and
-globulin fraction were
, respectively. It was observed that
-globulin was higher than other fractions. The most striking alternation with age was founded in the
-fractions. Also, it was observed that ratios of albumin to globulin in DongGyeong dogs were higher than on other dogs.
Determination of heavy metal contents in meats
Choi, Yoon-Hwa ; Kim, Yeon-Ju ; Lee, Kyung-Hye ; Kang, Young-Il ; Lee, Jung-Hark ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 299~302
This study was conducted to determine the content of heavy metals in meats available on the Korean markets. Trace metals (Pb, As and Cd) were detected in 4 kinds, 172 samples by inductively coupled plasma-mass spectrometry (ICP-MS). The values of heavy metals in meats were as follows; In beef, mean (minimum~maximum) values of the heavy metals were Pb: 0.075 (0.020~0.190)mg/kg, As: 0.010 (0.001~0.050)mg/kg and Cd: 0.43(0.07~2.11)
. In pork, mean (minimum~maximum) values of the heavy metals were Pb: 0.065 (0.012~0.171)mg/kg, As: 0.004 (ND~0.021)mg/kg and Cd: 0.43(0.08~1.09)
. In chiken, mean (minimum~maximum) values of the heavy metals were Pb: 0.060(0.016~0.211)mg/kg, As: 0.008 (ND~0.042)mg/kg and Cd: 0.42 (0.02~3.80)
. In duck, mean (minimum~maximum) values of the heavy metals were Pb: 0.070 (0.011~0.157)mg/kg, As: 0.005 (0.001~0.011)mg/kg and Cd: 0.87 (0.15~3.75)
. This results will be used as a basic data for the future legislation on the regulation and control of heavy metal in meats.
Effects of estrogen on the ovariectomized rat
Park, Young-Jae ; Chon, Seung-Ki ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 303~307
The aim of this study was to determine the effects of body weight, organ weight, hematological values and biochemical parameters by estrogen on the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group (n=10) received operation and no treatment. OVX+E2(n=10) received operation and estrogen. The body weight of each group increased, but that of OVX+E2 group decreased. There were very significant differences (P<0.001) of body weights between OVX+E2 group and the other groups. Also, the wight of important organs such as heart, liver, spleen and kidney was measured. The heart weight was significantly lower(P<0.05) in the OVX+E2 group than the Intact group. The liver weight of the OVX+E2 group was significantly different in comparison with those of the other groups. On the other hand, there were no significantly differences in the organ weight of spleen and kidney between the OVX+E2 group and the other remaining groups. The hematological values of WBC, RBC, MeV, MCH and MCHC were not significantly different among the groups. The serum total protein and albumin decreased significantly in the OVX+E2 group as compared to those in the intact group. But, there were no significant differences in Ca, IP and Mg among the groups. We conclude that estrogen was significantly decreased the body weight in the ovariectomized rats. Our findings suggest that estrogen may influence the process of lipid packaging and absorption in the ovariectomized rats.
Effects of L-carnitine on the ovariectomized rat
Chon, Seung-Ki ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 309~312
This was conducted to determine the effects of body weight, organ weight, hematological values and biochemical parameters by L-carnitine in the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group (n=10) received operation and no treatment. OVX+Carn group (n=10) received operation and L-carnitine. Body weight was significantly lower in OVX+Carn group than in all other groups. Also, organ weight such as heart, liver, spleen and kidney was measured. The heart and spleen weight were significantly lower in the OVX+Carn group than in the Intact and Sham group. The liver weight in the OVX+Carn group was significantly differences in comparison with those in the other groups. Also, there was significantly differences in the organ weight of kidney between in the OVX+Carn group and in the other groups. The hematological values of WBC, RBC, MCV, MCH and MCHC were no significant differences in any other groups. The total cholesterol, triglyceride and high density lipoprotein decreased significantly in the OVX+Carn group as compared to those in the OVX group. But, there were no significant differences in low density lipoprotein in any other groups. We conclude that L-carnitine enhanced the body weight in the ovariectomized rats. Our findings suggest that L-carnitine may influence the process of absorption of fat in the ovariectomized rats.
Prevalence of surgical affections with their clinical management in crocodiles (Crocodylus porosus) in Bangladesh
Arif, Abu S.M. ; Hashim, M.A. ; Runa, R.A. ; Chowdhury, E.H. ; Rahman, Md Siddiqur ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 33, issue 3, 2010, Pages 313~317
This study was conducted in the saltwater crocodile (Crocodylus porosus) for the occurrence of various surgical affections with their clinical management. During the period of January 2006 to June 2008, a total of 68 adult crocodiles were considered as reference population from the Reptiles Farm Ltd. at Bhaluka, Mymensingh. The occurrence of surgical affections of adult female (88.9%) was significantly (P<0.01) higher than male (11.1%). Prevalence of common surgical affections were wound: 72.2%, fibroma: 11.1% abscess: 5.6%, fracture: 5.6%, tail necrosis: 2.8%, myiasis: 2.8%. Wound was the highest among the affections. Among the identified wounds, biting wound was higher. In summer (57.7%) the occurrence of wound was higher compared to other seasons. In winter season (7.7%) the occurrence of abscess, myiasis, fibroma were higher compared to other seasons. Wounds and fibroma are the major surgical affections in salt water crocodile. The most common site of surgical affection was recorded in crocodile's tail (30.6%). It was suggested that minimal stress, allowing constant and easy access to water, removal of roots of the tree in the basking land of crocodiles pen, basking land of the pen is better filling up by sand, clinical surgical managements are essential for better health and production.