Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 34, Issue 4 - Dec 2011
Volume 34, Issue 3 - Sep 2011
Volume 34, Issue 2 - Jun 2011
Volume 34, Issue 1 - Mar 2011
Selecting the target year
Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2
Park, Chul ; Kim, Bum-Seok ; Kim, Yong-Hwan ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 1~4
DOI : 10.7853/kjvs.2011.34.1.001
A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.
Production and diagnostic application of monoclonal antibodies against infectious bursal disease virus
Ryu, Min-Sang ; Song, Yoon-Ki ; Lee, Seung-Chul ; Mo, In-Pil ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 5~12
DOI : 10.7853/kjvs.2011.34.1.005
Infectious bursal disease (IBD) caused by infectious bursal disease virus (IBDV) is a highly contagious viral disease in chicken. It causes heavy economic loss by immune suppression and high mortality. The IBDV, designated Avibirnavirus in the Family Birnaviridae, has a double-stranded RNA genome formed by two segments, segment A and segment B. Segment A encodes a 108 KDa polypeptide that is self-cleaved to produce pVP2, VP3 and VP4, and later pVP2 is cleaved to VP2. The VP2 contains the antigenic regions responsible for elicitation of neutralizing antibodies and VP3 is a major immunogenic protein of IBDV. In this study, monoclonal antibodies (MAbs) specific for IBDV were produced and characterized. All 15 MAbs were specific for IBDV and did not react with other viruses used in this study. The protein specificity of MAbs was determined by comparing the reactivity patterns of each MAb with IBDV VP2 and VP234 recombinant baculoviruses and Western blot analysis. As a result, 7 MAbs (1F5, 2C8, 2F4, 3C7, 4C3, 6F11, 6G5) and 5 MAbs (2A4, 2G2, 3F5, 3G2, 4F10) were specific for VP2 and VP3, respectively. The protein specificity of 3 MAbs (2B8, 3F7, 3F8) were not determined. Five (2C8, 2F4, 4C3, 6F11, 6G5) of the VP2-specific MAbs had a neutralizing activity against IBDV. Some MAbs reacted with IBDV-infected bursa of Fabricius by indirect fluorescence antibody (IFA) and immunohistochemistry (IHC) assay. The MAbs produced in this study would be used for diagnostic reagents for the detection of IBDV infection.
Application of a nested-polymerase chain reaction assay to differentiate the genotypes of porcine circovirus 2
Chu, Keum-Suk ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 13~18
DOI : 10.7853/kjvs.2011.34.1.013
The purpose of this study was to apply a nested-polymerase chain reaction (nPCR) assay to detect and differentiate PCV 2a and PCV 2b. The compared with nPCR and one-step PCR and nPCR showed more sensitive in the detection of PCV-2 from tissue and blood samples. The total of 52 tissue samples was collected from postweanning pigs from 2006 to 2010. All tissue samples showed positive for PCV-2 in one-step PCR and nPCR, followed by the nPCR in order to identify the genotypes of PCV-2. 2 samples (3.8%) showed positive for PCV 2a, and 35 samples were positive for PCV 2b (67.3%), 15 samples (28.9%) were positive the dual genotypes. In addition, 42 blood samples which were collected from the 5 different swine farms were compared figure out the detection rates of nPCR and one-step PCR. The PCV 2 was positive by one-step PCR in 21 samples (50.0%) and nPCR was positive in 37 samples (88.1%). The PCV 2 genotypes in blood samples and 32 samples (76.2%) were positive for PCV 2b and none were positive for PCV 2a, 5 samples (11.9%) were positive for dual genotypes. These results suggest that the nPCR is very efficient for genotyping blood samples and differentiating the genotypes of PCV-2 from field samples.
Prevalence of major legal communicable diseases in chicken and ducks in Jeonbuk province (2004~2008)
Hur, Boo-Hong ; Lee, Jeong-Won ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 19~29
DOI : 10.7853/kjvs.2011.34.1.019
Prevalence of major legal communicable diseases in chickens and ducks, which had occurred in Jeonbuk province from year 2004 to 2008. Total 283 farms 1,419,244 chickens and ducks have been affected by avian diseases. Specifically, fowl typhoid (FT) occurred in 92 farms 416,600 chickens, Marek's disease (MD) in 45 farms 145,563, duck virus hepatitis (DVH) in 31 farms 199,200, infectious bursal disease (IBD) in 27 farms 113,220, infectious bronchitis (IB) in 27 farms 280,300, low pathogenic avian influenza (LPAI) in 26 farms 78,495, avian mycoplasmosis in 16 farms 103,774, Newcastle disease (ND) occurred in 11 farms 61,052, avian encephalomyelitis (AE) in 7 farms 21,000, Pullorum disease (PD) occurred in 1 farm 40. According to total analysis about major legal communicable diseases, 1 species of first-class legal communicable diseases have occurred, 3 species of second-class and 6 species of third-class all adding up to 10 species. In the first-class diseases, Newcastle disease have occurred. Pullorum and fowl typhoid, duck virus hepatitis in the second-class have occurred and as third-class diseases, Marek's disease, Infectious bursal disease, Infectious bronchitis, avian mycoplasmosis, avian encephalomyelitis, low pathogenic avian influenza have occurred.
Mass mortality of Clostridium perfringens infection in Hanwoo
Lee, Joung-Won ; Kim, Ji-Young ; Kwak, Kil-Han ; Yuk, Hyun-Su ; Shon, Ku-Rye ; Koh, Won-Seuk ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 31~35
DOI : 10.7853/kjvs.2011.34.1.031
This is a case report that 24 heads of cattle suddenly died without clinical signs in a Hanwoo farm. The cause of death was Clostridium perfringens enterotoxemia resulted from them with feeding leftover food. The clinical signs were observed just before the death; increase of heart rate, shallow and rapid respiration, amyostasia, spasm and so forth. In autopsy, blood coagulation disorder, a little abdominal inflation, hepatomegaly and different size of red spots, congestion hemorrhage and undercurrent of bloody exudation were observed in the entire parts of small and large intestines. C. perfringens were isolated from the substantive organs, and a unique fragment of 405bp C. perfringens was amplified by PCR. Therefore, this case was diagnosed as enterotoxemia caused by
-toxin of C. perfringens A type.
Detection of CTX-M and TEM type extended-spectrum β-lactamases in Escherichia coli isolated from livestocks in Korea
Cho, Jae-Keun ; Sung, Myung-Suk ; Kim, Jin-Hyun ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 37~43
DOI : 10.7853/kjvs.2011.34.1.037
This study was conducted to investigate the prevalence and genotypes of extended-spectrum
-lactamase (ESBL) in 377 Escherichia coli isolated from healthy and sick animals. Two isolates (0.5%), each of which were isolated from diseased swine and chicken, respectively, were confirmed as ESBL producing isolates by double disk synergy test, and showed a multidrug resistant phenotype. Minimum inhibitory concentration of cefotaxime for the two ESBL producing isolates were 3~4 times higher than those of ceftazidime, respectively. By PCR and sequencing, one isolate from swine have both
, and one isolate from chicken have
. Also, these genes were transferred to E. coli J53 by conjugation. These two isolates showed unrelated pulsed-field gel electrophoresis. To our knowledge, this is the first time that
gene was identified in E. coli isolated from animals in Korea. These results suggest more prudent use of third- generation cephalosporins, and surveillance and monitoring for ESBL producing E. coli in both animals and their environments should be necessary.
Prevalence of the antimicrobial resistance and resistance associated gene in Salmonella spp. isolated from pigs and cattle in slaughterhouse
Hah, Do-Yun ; Ji, Dae-Hae ; Jo, Sang-Rae ; Park, Ae-Ra ; Jung, Eun-Hee ; Park, Dong-Yeop ; Lee, Kuk-Cheon ; Yang, Jung-Wung ; Kim, Jong-Shu ; Kim, Hye-Jung ; Jung, Jong-Hwa ; Song, Ick-Hyun ; Kim, Ae-Ran ; Lee, Ji-Youn ; Kim, Young-Hwan ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 45~54
DOI : 10.7853/kjvs.2011.34.1.045
This study was conducted to investigate the distribution of Salmonella spp. from pigs and cattle in slaughterhouse, the antimicrobial resistance pattern and the prevalence of resistance genes of isolates. A total of 640 fecal samples from pigs and cattle in slaughterhouse were collected for isolation of Salmonella spp.. Isolation rate was revealed as 15% in pigs and 1.6% in cattle. As result of serotyping, group B (56.6%) were identified as most common in pigs and cattle isolates, in order of group C (24.5%) and group E (15.1%). S. Typhimurium (50.9%) was most common serotype. The major serotypes were in order of S. Rissen and S. London (11.3%) and S. Riggil (7.6%). In antimicrobial test, all isolates were demonstrates susceptibility to nitrofurantoin. But isolates were revealed resistance other antibiotics in order of tetracycline (64.6%), streptomycin (68.3%), ampicillin and amoxicillin (56.3%) and spectinomycin (47.9%). With polymerase chain reaction, antimicrobial resistance gene strA (75.0%) and aadA1 (3.1%) were detected in streptomycin resistance isolates and tetA (94.3%) and tetB (11.3%) gene were detected in tetracycline resistant isolates, but tetG was not detected. Class 1 integron gene was detected in all Salmonella isolates.
Dermatopathology in respect to bovine cutaneous streptothricosis with clinical observations at Dinajpur of Bangladesh
Juli, Sogra Banu Mst. ; Hoque, Md. Fazlul ; Zohara, Begum Fatema ; Harun-Ur-Rashid, S.M. ; Islam, Md. Nazrul ; Rahman, Md. Siddiqur ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 55~61
DOI : 10.7853/kjvs.2011.34.1.055
Dermatopathologic findings on cutaneous streptothricosis (bovine dermatophilosis) were conducted in Dinajpur of Bangladesh for one year. The animals were examined clinically at hospital during the physical visit of the affected animals and clinical manifestations and patients' history were recorded. The gross lesions were systematically examined, noted and categorized. An impression smear was made by the skin scraps, stained with Gram's stain and examined microscopically for the presence of causative bacteria. The suitable sizes of skins of 3 animals typically affected with dermatophilosis were collected surgically from the animals, and prepared for the histopathological study. The cardinal clinical features and gross lesions were characterized as paint-brush cutaneous surface with matted hairs, scab and crust formation, roughened coat; istopathologically as hyperkeratosis, parakeratosis, typical "palisading encrustations", extensive proliferation of coccoid shaped, bluish coloured bacteria (zoospores) in the cells of the outer rootsheath of the hair follicles, separation of keratinized layer from the epidermal epithelium, epidermal hyperplasia, densely cellular dermis with moderate destruction of glandular structures. Coccoid shaped gram positive organisms which form multidimensional arrangement were also observed by impression smear. The clinical signs, gross lesions, morphology of the organisms, staining properties, and histopathological features of the affected skin were diagnostic criteria for the bovine cutaneous streptothricosis.
Effects of vitamin E and selenium (Selevit) on the orchidectomized rats
Chon, Seung-Ki ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 63~68
DOI : 10.7853/kjvs.2011.34.1.063
The present study was devised to determine the effects of body weight, organ weight, hematological values and biochemical parameters by vitamin E and selenium (Selevit) on the Orch rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. Orch group received operation and no treatment. Orch+Selevit received operation and Selevit. The body weights of each group increased, but that of the Orch+Selevit group were significantly lower than those of all the other groups. There were significant differences (P<0.001) of body weights between Orch+Selevit group and all the other groups. Also, organ weights such as heart, liver, spleen and kidney were measured. The heart weights were significantly lower (P<0.001) in the Orch+Selevit group than in Intact and Sham group. The liver weights in the Orch+Selevit group were significantly differences (P<0.001) in comparison with those in the Intact and Sham groups. The kidney weights in the Orch+Selevit group were significantly differences (P<0.01, P<0.001) in comparison with those in all the other groups. On the other hand, there were no significantly differences in the organ weights of spleen between the Orch+Selevit groups and the any other groups. The number of white blood cell (WBC) was significantly higher (P<0.05) in the Orch+Selevit group than in all the other groups. The hematological values of red blood cell (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were not significantly different in any of the groups. The concentrations of serum total protein, albumin and alkaline phosphatase increased significantly (P<0.05, P<0.01) in the Orch+Selevit group as compared to that in the Orch group. However, there were no significant differences in AST and ALT in any other groups. We conclude that Selevit was significantly decreased the body weight in the orchidectomized rats. Our findings suggest that Selevit may influence the process of lipid packaging and absorption in the orchidectomized rats.
Effects of L-carnitine on the orchidectomized rats
Chon, Seung-Ki ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 69~73
DOI : 10.7853/kjvs.2011.34.1.069
This was conducted to determine the effects of body weight, organ weight, hematological values and biochemical parameters by L-carnitine (Carn) on the orchidectomized (Orch) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. Orch group received operation and no treatment. Orch+Carn received operation and L-carnitine. The body weights of each group increased, but that of the Orch+Carn group were significantly lower than those in all the other groups. There were significant differences (P<0.05, P<0.001) of body weights between Orch+Carn group and all the other groups. Also, organ weights such as heart, liver, spleen and kidney were measured. The heart weights were significantly lower (P<0.001) in the Orch+Carn group than those in Intact and Sham groups, respectively. The weights of liver and kidney in the Orch+Carn group were significantly differences (P<0.001) in comparison with those in all the other groups. Also, the spleen weights were significantly lower (P<0.05) in the Orch+Carn group than those in Intact and Sham groups, respectively. The hematological values of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were significantly differences (P<0.05, P<0.01, P<0.001) in comparison with those in all the other groups. On the other hand, the hematological values of white blood cell (WBC), red blood cell (RBC) and mean corpuscular hemoglobin concentration (MCHC) were not significantly different in any other groups. The concentrations of total cholesterol (T-chol), triglyceride (TG) and high density lipoprotein (HDL) decreased significantly (P<0.05) in the Orch+Carn group as compared to those in the Orch group. We conclude that L-carnitine was significantly decreased the body weight in the orchidectomized rats. Our findings suggest that L-carnitine may influence the process of lipid packaging and absorption in the orchidectomized rats.
Renal effect of experimental feeding of melamine and cyanuric acid in different concentrations on Japanese catfish (Silurus asotus)
Han, Se-Hee ; Heo, Gang-Joon ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 75~79
DOI : 10.7853/kjvs.2011.34.1.075
The recent outbreak of renal failure in infants in China and in animals in USA and Europe has been determined to be caused by melamine adulterated in the food. In the course of the investigation, cyanuric acid was identified in addition to melamine in the offending food. Fish feeds were also recently found to be contaminated with melamine. The purpose of this study was to characterize the histopathological effect and toxicity potential of different concentrations of melamine and cyanuric acid in the kidney of Japanese catfish (Silurus asotus). The fish were administered melamine and cyanuric acid in combination at the concentrations of 12.5, 25, 50, 100 and 200 mg/kg/day for 3 days by single oral administration dissolved in carboxymethyl cellulose. The results showed that renal crystals were observed in renal tubules and collecting ducts at the concentration over 25 mg/kg dose group and the number of crystals in kidney were in proportion to the concentrations of melamine and cyanuric acid.
Survey of beekeeper's consciousness for diagnosis and treatment on disease of honey-bees
Chung, Nyun-Ki ; Hwang, In-Su ; Park, Sang-Guk ; Jeong, Gwang-Ug ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 81~86
DOI : 10.7853/kjvs.2011.34.1.081
This study was designed to evaluate the beekeeper's consciousness for diagnosis and treatment on a disease of honey-bee in Daejeon and Jeollanamdo in Korea. The questionnaire consisted of 14 questions. Of 231 randomly selected respondents between February and May 2010, 47.6% was consult a 'club (colleague)', 89.2% treated a 'doctoring oneself', 84.4% was recognize a bee as 'domestic animal', 77.5% was not recognize a veterinarian as field of treatment, 38.5% was acquire know-how by a beekeeper. Therefore these results suggest that the beekeepers are relying a lot on individuals experience for the diagnosis and treatment on the disease of honey-bee, the apiculture techniques was gained much by oneself. Korean beekeeping association was played a role as well, but related-university, agriculture research & extension services and veterinary service laboratory was not played a role in the country.
Effects of the blood chemistry of honeybee (Apis mellifera L.) venom on the Hanwoo calves
Oh, Baeg-Young ; Han, Sang-Mi ; Oh, Young-Ik ; Kim, Soon-Tae ;
Korean Journal of Veterinary Service, volume 34, issue 1, 2011, Pages 87~93
DOI : 10.7853/kjvs.2011.34.1.087
This study was conducted to investigate effects of honeybee venom (Apis mellifera L. BV) on the calving, the growth performance and blood chemistry occurrence of Hanwoo calves. A total of twenty, a 3 day old male experimental calves were allocated into four groups, BV treated (0.05 mg/kg, 0.1 mg/kg and 0.2 mg/kg BV, n=5, respectively) and non-treated (n=5, Control) of Hanwoo calves in Icheon, Kyonggi province. Treatment of BV in calves increased body weight and weight gain during 60 days compared with control group. The concentrations of IgG in blood were significantly increased in BV groups compared with control at 60 days after treatment with BV. Cholesterol and glucose concentrations in BV group were significantly lower with control at 60 days. There were no differences in plasma biochemical components including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphate (AKLP), total protein, albumin, globuline, total bilirubin, creatinine, blood urea nitrogen (BUN) and
. In conclusion, the BV treatment had effect on growth, the score of the calf scours and blood biochemical profile.