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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 34, Issue 4 - Dec 2011
Volume 34, Issue 3 - Sep 2011
Volume 34, Issue 2 - Jun 2011
Volume 34, Issue 1 - Mar 2011
Selecting the target year
Analysis of complete genome sequence of foot-and-mouth disease (FMD) Asia1 vaccine strain
Lee, Yeo-Joo ; Chu, Jia-Qi ; Lee, Seo-Yong ; Kim, Su-Mi ; Lee, Kwang-Nyeong ; Ko, Young-Joon ; Lee, Hyang-Sim ; Cho, In-Soo ; Nam, Seok-Hyun ; Park, Jong-Hyeon ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 95~102
DOI : 10.7853/kjvs.2011.34.2.095
Foot-and-mouth disease (FMD) is one of the most infectious diseases affecting cloven-hoofed animals including cattle, sheep, goats, and pigs. Seven serotypes of foot-and-mouth disease virus with multiple subtypes within each serotype have been identified until now. In particular, it has been demonstrated that the outbreak of the serotype Asia1 reported from China, Mongolia and North Korea since 2005 is mostly classified into genetic group V. Though it has been recommended that Asia1 Shamir strain can be used as a high priority vaccine by World References Laboratory for FMD, the complete nucleotide sequences of the strain has not yet been determined. In this study, to be prepared for Asia1 type viruses that may be brought into Korea, the complete genome sequence of this vaccine strain Asia1 Shamir including its 5' and 3' non-coding region was identified.
Analysis of protective genotype of foot-and-mouth disease (FMD) Asia1 vaccine
Lee, Yeo-Joo ; Chu, Jia-Qi ; Lee, Seo-Yong ; Kim, Su-Mi ; Lee, Kwang-Nyeong ; Ko, Young-Joon ; Lee, Hyang-Sim ; Cho, In-Soo ; Nam, Seok-Hyun ; Park, Jong-Hyeon ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 103~109
DOI : 10.7853/kjvs.2011.34.2.103
Asia1/Shamir that has been recommended by World Reference Laboratory for foot-and-mouth disease (FMD) is used as a vaccine strain, and is being prepared in many countries including Korea. Although it is assumed that vaccine strain Asia1/Shamir has a wide antigenicity, sufficient molecular biological analysis has not been accomplished yet. Complete genome sequence analysis showed that the region with the most severe variations was 1D region of structural protein-coding sequence; particularly amino acid 141~157 residues in 1D region RGD sites for binding to susceptible cells. In addition, five amino acids in 1D region were identified as characteristic sites that are different from other known Asia1 viruses. Asia1/Shamir strain was shown to be genetically similar to group VI that had occurred in the Middle East, but showed low level of genetic similarity to the group V viruses that had occurred in the Southeast Asia and China. It is considered that, if these viruses, group I and II including group V are introduced into Korea, care would be paid in case of inoculating the vaccine strain Shamir available in Korea.
A survey of viruses associated with reproductive failure in boar semen in Korean artificial insemination centers
Kim, Yeong-Hun ; Chun, Bong-Su ; Kim, Sung-Jae ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 111~116
DOI : 10.7853/kjvs.2011.34.2.111
Artificial insemination (AI) of swine is a very useful reproductive tool and that offers convenience in the Korean swine industry. Since many viruses have been reported to be excreted through boar semen, we investigated the presence of antibodies and antigens against viruses causing reproductive failure in semen of boar in 349 semen samples collected from six Korean AI centers. Viral antigens were detected by polymerase chain reaction (PCR) or reverse transcription-PCR predominantly. The results was as follows. The major reproductive failure causing factor was porcine circovirus type 2 (PCV2), followed by porcine reproductive and respiratory syndrome virus (PRRSV) (
=166.64, P<0.001). PCV2 and PRRSV, Japanese encephalitis virus (JEV), encephalomyocarditis virus (EMCV) was detected in 73 samples (20.9%), 44 samples (12.6%), 4 samples (1.1%), 3 samples (0.9%), respectively and porcine parvovirus in one sample (0.3%) Classical swine fever virus (CSFV), bovine viral diarrhea virus and Aujeszky's disease virus (ADV) were not detected. Enzyme-linked immunosorbent assay was carried out in 111 serum samples from three AI centers. In most pigs, antibodies response was showed prominently in CSFV (105 sera, 94.6%) (
=82.580, P<0.001), followed by, in PRRSV (100 sera, 90.1%), PCV2 (92 sera, 90.1%), and PPV (8 sera, 82.9%). ADV antibody was not detected. Thus, the experimental results will be used for the base data, with respect to the state of viral stillbirth in general pig farms, as well as AI centers and breeding farms in Korea.
Detection and prevalence of viral pathogens from aborted fetuses and stillborn piglets in Korea
Lee, Won-Gwang ; Kim, Sung-Jae ; Kim, Yeong-Hun ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 117~123
DOI : 10.7853/kjvs.2011.34.2.117
This experiment was conducted to investigate the presence of recognized abortifacient viruses from aborted fetuses and stillborn piglets in cases of reproductive failure in sows by PCR. A total of 219 samples of aborted fetuses or stillborn piglets, submitted to the School of Veterinary Medicine of Kangwon National University between 2006 and 2009 May, were collected from 5 provinces in Korea. Abortifacient virus infections were detected in 82 (37.4%) out of 219 aborted fetuses or stillborn piglets as well as on 39 (40.2%) out of 97 pig farms. The major viral infections were porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV) and Aujesky's disease virus (ADV) for which 46 (21%), 19 (8.6%) and 16 (7.3%) were positive, respectively, with 9 fetuses had complicated infection of PCV2+PRRSV or ADV or both. And 8 (3.6%) for SIV, 3 (1.3%) for PPV and 1 (0.4%) for JEV were positive as minor viral infection. The results suggest that PCV2, PRRSV, ADV is apparently the most important viral infectious agents associated with fetal infection leading to abortion or stillbirth in Korea. SIV, PPV and JEV might have a minor impact on reproductive disease.
Prevalence of porcine group A, C rotavirus and calicivirus infections in the farm located in Incheon area
Jeong, Cheol ; Ra, Do-Kyung ; Kim, Kyoung-Ho ; Han, Tea-Ho ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 125~131
DOI : 10.7853/kjvs.2011.34.2.125
The present study was aimed to investigate the prevalence of porcine group A rotavirus, group C rotavirus and calicivirus from the 46 pig farms located in Incheon area. Group A rotavirus was detected in 16 (5.3%) from 8 farms (17.4%), and group C rotavirus was determined in 17 samples (5.7%) from 6 farms (13.0%). Porcine calicivirus was also detected in fecal samples [11 samples (3.7%) from 2 farms (4.3%)]. Correlation analysis was carried out among porcine enteric viruses and clinical signs, herd size and temperature on the basis of these results. The occurrence of porcine group A rotavirus, group C rotavirus and calicivirus infections in Incheon area was not associated with season and temperature. Especially, group C rotavirus was also detected in the pigs without any clinical symptoms.
Characterization of the infection pattern of porcine respiratory disease complex (PRDC) in the northern area of Gyeongsangnam-do, Korea
Kim, Min-Hee ; Park, Jong-Sik ; Lee, Min-Kweon ; Kim, Chul-Ho ; Shin, Jung-Sup ; Kim, Hyun-Joon ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 133~138
DOI : 10.7853/kjvs.2011.34.2.133
The prevention of porcine respiratory disease complex (PRDC) is very important because of its high infection-rates in the swine farms and the economic impact in swne industry in Korea. To control the prevalence of PRDC, it is important to know about infection patterns of it. Therefore, this study aimed to investigate the infection patterns of PRDC in the northern area of Gyeongsangnam-do. To this end, the infection of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), Actinobacillus pleuropneumoniae (APP), Mycoplasma hyopneumoniae (MH), and Swine influenza virus (SIV) were examined using 120 pig lung tissues by PCR analysis. As a result, single pathogen positive specimens were 25.0% and the others (75.0%) were turned out to be PRDC with at least two pathogens. Among PRDCs, 50 specimens (41.7%) was infected with PRRSV, PCV2, MH and SIV. Ten specimens (8.3%) showed triple infections of PRRSV, PCV2 and MH. Double infected specimens for PRRSV and PCV2 were 10 (8.3%), and for PCV2 and APP were 20 (16.7%).
The comparison of pathogenicity in the SPF chickens challenged with avian reovirus and/or fowl adenovirus
Min, Kyeong-Cheol ; Choi, Dong-Myong ; Kim, Ji-Yea ; Jeon, Eun-Ok ; Byun, Sung-Hwan ; Mo, In-Pil ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 139~148
DOI : 10.7853/kjvs.2011.34.2.139
Avian reovirus (ARV) and fowl adenovirus (FAdV) were evaluated for pathogenicity in specific pathogen free (SPF) chickens. ARV was isolated from the broilers with history of malabsorption syndrome (MAS). FAdV was isolated from the layer breeders with inclusion body hepatitis and hydropericardium syndrome. Total 6 inoculated groups including 1 un-inoculated group were organized and inoculated with the ARV and/or FAdV by oral route. The minimal pathological lesions and lower viral gene detection rates were present in the ARV inoculated groups compared to those of FAdV or ARV/FAdV inoculated groups. Common gross lesions in the ARV inoculated group were distended intestine with foamy contents and in the FAdV group there were foamy cecal contents and hydropericardium among the evaluation methods such as gross and histological lesion, viral gene detection, body weight and serum chemistry, histopathological lesion score was reliable especially in the liver lesions such as hepatic necrosis and lymphocytic infiltration. However, we did not success to evaluate the synergetic effect of mixed infection of ARV and FAdV in this study. Therefore, we need further study to reproduce malabsorption syndrome of ARV infection using different viral agent such as rotavirus and using different dose of virus.
Seroprevalence of chicken infectious anemia virus in breeder and broiler chicken in Jeonbuk Iksan area
Um, Sung-Shim ; Kwak, Kil-Han ; Lee, Joung-Won ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 149~152
DOI : 10.7853/kjvs.2011.34.2.149
From January to November in 2010, a result of chicken infectious anemia virus (CIAV) antibodies ELISA test about 500 number in 25 broiler breeder farms and 500 number in a broiler chickens slaughterhouse from 25 farms in Iksan area, Jeonbuk. The result of 100% (25/25) positive rate in broiler breeder farms group and 98.6% (493/500) positive rate from each individual. In each week-age group, 98.8% of 18~20 weeks and 98.3% of 21~24 weeks showed positive without any significant differences. In slaughterhouse case, broilers from the farms showed 36.0% (9/25) positive rate and each individual showed 20.4% (102/500) positive rate.
Serosurvey for antibodies against brucellosis in pigs
Hur, Jin ; Baek, Byeong-Kirl ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 153~157
DOI : 10.7853/kjvs.2011.34.2.153
In order to investigate serum antibodies for detection of brucellosis in pigs, a total of 1208 sera were tested by Rose Bengal test (RBT), the standard tube agglutination test (STAT) and competitive ELISA (cELISA). The sera were collected from pigs of Gyeonggi, Chungnam, Chungbuk, Jeonnam and Jeonbuk, provinces during the period 2002 to 2004. All the sera were screened by RBT, and were confirmed by STAT and cELISA. Among 1208 sera, 26 sera (2.2%) were positive in screening test. All the 26 positive sera were positive by STAT, while all the sera were negative by cELISA. On the basis of this study, farmed pigs may be exposed to Brucella species. Furthermore, these results suggest that establishment of diagnoses for detection of porcine brucellosis is necessary.
Development of ELISA for detection of canine brucellosis
Hur, Jin ; Baek, Byeong-Kirl ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 159~166
DOI : 10.7853/kjvs.2011.34.2.159
This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately
colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.
Antimicrobial effects of garlic extract against pathogenic bacteria
Lee, Woo-Won ; Son, Soo-Kyeong ; Lee, Gang-Rok ; Kim, Geum-Hyang ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 167~178
DOI : 10.7853/kjvs.2011.34.2.167
Garlic (Allium sativum L.) has long history of reputed value and actual use for its medicinal, antimicrobial and pesticidal properties. This study was conducted to find possible developments to natural food preservatives and natural antimicrobials from garlic extracts. The antimicrobial activities of raw garlic extract, heat, pH, temperature against pathogenic bacteria were investigated. E. coli, S. Enteritidis, L. monocytogenes, and S. aureus exhibited antimicrobial activities at 20% garlic extract, but no antimicrobial activity was seen in E. faecium. Raw garlic extract and garlic extract heated for 2 min at
showed strong antimicrobial activities, but the antimicrobial activity of garlic extract heated for 10 min at
was much less. The antimicrobial activities of 50% garlic extract adjusted pH 4.0~7.0 showed much the same, but the antimicrobial activities decreased at pH 8.0 or higher. The antimicrobial activities by storage
of garlic extract showed much the same. When five strains were cultured for 72 hr at
in the TSB containing 1~10% garlic extract, viable cell number of five strains were decreased to
CFU/ml even at 1% or 2.5% (E. faecium) after 24 hr, but later increased to
CFU/ml after 72 hr. When five strains were cultured for 21 day at
in the TSB containing 1%, 2.5%, 5%, and 10% garlic extract, viable cell number of E. coli, S. Enteritidis, and S. aureus were decreased to
CFU/ml after 21 day, respectively, but L. monocytogenes and E. faecium increased to
CFU/ml after 21 day.
Seroprevalence of antibodies to Neospora caninum in cattle at Seoul slaughtering center
Kim, Jee-Eun ; Son, Jang-Won ; Yang, Yun-Mo ; Jeon, Hae-Chang ; Jin, Kyung-Sun ; Kim, Kyu-Hyun ; Shin, Bang-Woo ; Lee, Jung-Hark ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 179~185
DOI : 10.7853/kjvs.2011.34.2.179
Neospora caninum is now recognized as one of the important causes of abortion in cattle worldwide, but there was no report on seroprevalence of antibodies to N. caninum in cattle at Seoul slaughtering center. Serum samples from 610 Korean indigenous cattle (Hanwoo) and 246 Holstein cattle from 452 farms located all over the country, Korea, were tested for N. caninum antibodies using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Seven of 610 (1.1%) Hanwoo and 54 of 246 (22.0%) Holstein cattle were seropositive.
Coinfected cases with equine herpesvirus type 1, 4 and Streptococcus equi subsp. zooepidemicus in throughbred horse
Kim, Seong-Guk ; Cho, Gil-Jae ; Cho, Min-Hee ; Kim, Young-Hoan ; Lee, Hong-Young ; Choi, Jeong-Hye ; Kim, Jeong-Hwa ; Choi, Seong-Kyoon ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 187~190
DOI : 10.7853/kjvs.2011.34.2.187
The Thoroughbred horse was an approximately 4-years-old castrated male with highly emaciation, nasal epistaxis and subsequently died. Gross necropsy revealed epistaxis and hyperemia on the lung, multiple hemorrhage in muscle, and liver was focally attached to the peritoneum with fibrin. According to polymerase chain reaction (PCR), Equine herpes virus type 1 and 4 (EHV type 1, 4) was detected in the lung and trachea. In bacterial culture from kidney, liver, spleen, muscle and blood, Streptococcus equi subsp. zooepidemicus was isolated. Based on the gross lesion and PCR, this horse was diagnosed as EHV type 1, 4 and S. zooepidemicus coinfection.
Hemophilia B (factor IX deficiency) in a Labrador retriever dog
Kim, Jun-Hwan ; Noh, Dong-Ho ; Song, Ru-Hui ; Lee, Da-Mi ; Cho, Ho-Seong ; Yu, Do-Hyeon ; Park, Jin-Ho ; Park, Chul ;
Korean Journal of Veterinary Service, volume 34, issue 2, 2011, Pages 191~193
DOI : 10.7853/kjvs.2011.34.2.191
A 3-month-old intact male, Labrador retriever was presented with the history of coagulopathy and anemia. The results of initial screening tests of the hemostatic system yielded a tentative diagnosis of hemophilia. Activated partial thromboplastin time (APTT) was distinctly prolonged (106 seconds) and prothrombin time (PT) was not detected due to markedly prolonged test time. Whole blood transfusions (20 me l/kg body weight) were carried out prior to assays of coagulation factor. After transfusion, the patient recovered well and hemorrhage ceased. Blood samples were assessed for coagulation factor activity. The patient showed markedly low factor IX coagulation activity (5%, reference range: 7~140%) and was diagnosed with hemophilia B. After recovery, the patient was discharged from the hospital. However, 4 months later the patient was re-hospitalized for recurrence of the initial symptoms. The owner did not want to pursue further treatment and the patient died of respiratory distress two days later.