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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 34, Issue 4 - Dec 2011
Volume 34, Issue 3 - Sep 2011
Volume 34, Issue 2 - Jun 2011
Volume 34, Issue 1 - Mar 2011
Selecting the target year
Seroprevalence survey of swine influenza virus (H1N1, H3N2) in pigs in Gyeongnam area
Jang, Eun-Hee ; Hah, Do-Yun ; Park, Dong-Yeop ; Lee, Kuk-Cheon ; Heo, Jung-Ho ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 195~200
DOI : 10.7853/kjvs.2011.34.3.195
Swine influenza is an acute respiratory disease prevalent in pig-growing areas all around the world and plays the roles of an intermediate host to be transmitted to mammals including human beings through a genetic recombination with the avian influenza virus. Recognizing that people could be contracted with swine influenza, this study set out to investigate the seroprevalence of individual and multiple infections with two subtypes (H1N1 and H3N2) of the swine influenza virus in pig farms in the Gyeongnam region according to age, area, and season, as well as to provide basic data for the prevention and control of swine influenza. Used in the study were total 904 swine sera that were not vaccinated against the influenza gathered from the pig farms in the Gyeongnam region from November, 2009 to October, 2010. HerdChek SIV (H1N1, H3N2) ELISA kit (IDEXX Laboratories, USA) was used for antibody testing against swine influenza. The test results show that 370 sera (40.9%) were infected with either H1N1 or H3N2 with 37.3% (337 sera) being contracted with H1N1, 13.1% (118 sera) with H3N2, and 9.4% (85) with both H1N1 and H3N2.
The biochemical and molecular characteristics of Streptococcus equi subsp. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea
Choi, Seong-Kyoon ; Kim, Seong-Guk ; Cho, Gil-Jae ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 201~208
DOI : 10.7853/kjvs.2011.34.3.201
Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP (S. zooepidemicus protective protein) gene, CNE (collagen binding protein) gene and ISR (16s rRNA intergenic spacer region) gene using ABI Prism
Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.
Biochemical characteristics and antimicrobials susceptibility of pathogenic Streptoccus spp. isolated from respiratory tract of Thoroughbred horses in Jeju, Korea
Choi, Seong-Kyoon ; Kim, Seong-Guk ; Cho, Gil-Jae ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 209~216
DOI : 10.7853/kjvs.2011.34.3.209
This study carried out to investigate the pathogenic Streptococcus spp. isolated from respiratory tract of Thoroughbred in Jeju province. The specimens were collected from nasal mucosa using a culture swab from 113 Thoroughbred horses. Suspected colonies were selected onto blood and MacConkey agar plate, and identified by standard biochemical properties using Vitek 2 system and PCR method. In this study, we isolated S. equi (n=6), S. zooepidemicus (n=31), S. equisimilis (n=5), S. dysgalactiae (n=2), S. agalctiae (n=1), non identified
-hemolytic Streptococcus spp. (n=1) from Thoroughbred horses. In antimicrobial susceptibility test, it showed a high sensibility in the most of antimicrobial except for neomycin, streptomycin, spectinomycin, erythromycin and clindamycin. These results will provide the basic information to establish control measures for the treatment and prevention of respiratory disease by pathogenic Streptococcus spp. in Thoroughbred horses in Korea.
Serotypes, antimicrobial resistance of Salmonella spp. and plasmid profiles, phage types, PFGE of S. Enteritidis and S. Typhimurium isolated from ducks in Daegu-Gyeongbuk province
Cho, Jae-Keun ; Kang, Min-Su ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 217~226
DOI : 10.7853/kjvs.2011.34.3.217
Salmonella spp. is of increasing public health concern as causative pathogens of food poisoning. The aim of this study was to investigate the serotypes and antimicrobial resistance pattern of Salmonella spp. isolated from duck farms in Daegu-Gyeongbuk province. Also, S. Enteritidis and S. Typhimurium isolates were further examined for plasmid analysis, phage typing and pulsed-field gel electrophoresis (PFGE). A total of 34 Salmonella spp. (16.4%) were isolated from duck farms and ten serotypes were identified. The predominant serotypes were S. Typhimurium (23.5%) S. Fyris (17.6%) and S. Haardt (11.8%), S. Agona and S. Enteritidis (respectively 8.8%). Of 34 Salmonella isolates, 15 (44.1%) isolates were resistant to at least one antimicrobial agent and multiple resistance (resistance to more than 4 drugs) was observed in 9 strains (26.5%). The high resistance was found to streptomycin (32.4%), tetracycline (29.4%), ampicillin, kanamycin and nalidixic acid (respectively, 26.5%), all Salmonella isolates were susceptible to cefoxitin, cefotaxime, gentamicin, amikacin and ciprofloxacin. All S. Enteritidis and S. Typhimurium isolates were found to contain only one plasmid (ca. 54 or 55kb, respectively). Among the S. Enteritidis isolates, two phage types were found, PT32a and PT1c, respectively, one isolates did not react with any of the phages used. Whereas, all S. Typhimurium isolates were RDNC (reacts but does not conform). PFGE showed to be a useful typing method better than plasmid analysis and phage typing for discrimination of isolates especially, S. Typhimurium isolates. Our results indicated that the serotypes of Salmonella isolates are widely distributed in duck farms, further epidemiological studies should be carried out.
Genotyping of Brucella abortus isolated from cattle in Gyeongbuk province by MLVA
Kim, Seong-Guk ; Kim, Young-Hoan ; Lee, Hong-Young ; Choi, Jeong-Hye ; Choi, Seong-Kyoon ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 227~234
DOI : 10.7853/kjvs.2011.34.3.227
Brucella (B.) abortus is a facultative intracellular pathogen that infects a wide variety of animal species and human. Brucellosis is the zoonosis and an extremely important disease around the world. Although the eight species can be differentiated by conventional phenotypic tests, these species display a high degree of DNA homology in DNA-DNA hybridization assay (>90%). Various methods have been established for genotyping of Brucella species, but most of analytical methods are lack reproducibility and limited capability to differentiate them. The attempt of this study was to evaluate multiple-locus VNTR analysis (MLVA) for use of epidemiological trace-back analysis in bovine brucellosis. Ninety-four B. abortus isolates from Gyeongbuk province during 2006~2010 were analyzed using 16 VNTR locus. High resolution automatic capillary electrophoresis system was used for more throughput, simpleer, faster, and better discriminable than conventional gel electrophoresis. As a result, 13 different genotypes were identified from 94 B. abortus isolates. MLVA could contribute to epidemiological trace-back analysis of bovine brucellosis.
Effects of Lactobacillus acidophilus on innate immunity
Kang, Shin-Seok ; Byeon, Hyeon-Seop ; Kim, Jeong-Tae ; Lee, Ran ; Kang, So-Jeong ; Jung, Ho-Sung ; Kang, Sung-Ho ; Lee, Jae-Dong ; Kim, Dong-Hee ; Kang, Shin-Kwon ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 235~243
DOI : 10.7853/kjvs.2011.34.3.235
Probiotics have many effects such as antihypertensive, prevention of cancer, antioxidation, reduction of dermatitis symptoms, improvement of mineral absorption, reduction of allergic symptoms, and decrease of cholesterol, However, the main role of probiotics is that they balance intestinal microbials proportion. L. acidophilus is one of probiotics and microflora in intestine. It has an acidification activity, aroma production, texture formation and probiotics properties. We studied on the roles of L. acidophilus in mice. In this study, body weights of mice were decreased when administration of L. acidophilus (
CFU) and swimming ability has been raised than a normal group after feeding on L. acidophilus (
CFU). After taking L. acidophilus (
CFU), total white cells were increased than a normal group; hemoglobin and thrombocytes were increased. The level of cholesterol and triglyceride were decreased in blood analysis. We knew L. acidophilus is related to innate immune system. We found out the secretion of cationic peptide was increased in the Lysoplate assays as a result of L. acidophilus (
CFU) administration. Appearance rate of lysozyme was also increased than the normal group on an immunohistochemistry stain. We confirmed L. acidophilus contributes to host health through innate immune system stimulation. L. acidophilus more than
CFU are thought to be beneficial for the host health and prevention of intestinal diseases in field condition.
Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in cattle in northern area of Gyeongnam
Lee, Min-Kwon ; Park, Jong-Sik ; Kim, Min-Hee ; Park, Dong-Yeop ; Kim, Cheol-Ho ; Kim, Guk-Heon ; Cho, Jae-Hyeon ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 245~250
DOI : 10.7853/kjvs.2011.34.3.245
In the present study, we carried out a seropositive survey of Neospora (N) caninum and Toxoplasma (T) gondii in cattle at northern area of Gyeongnam province, Korea. Serum sample was obtained from Holstein cattle (n=248, 44 farms in 5 counties) and Korean indigenous cattle (n=120, 23 farms in 4 counties). All 368 sera were tested for antibodies to N. caninum and T. gondii by an enzyme-linked immunosorbent assay (ELISA). Seropositive of N. caninum at farm and heads were 41.8% (28/67) and 12.8% (47/368), respectively. Twenty-five cattle (6.8%) were seropositive to T. gondii, and eighteen (26.9%) of 67 studied farms had cows seropositive to T. gondii. The seroprevalence of Holstein cattle was much higher than that of Korean indigenous cattle. There was no apparent association of T. gondii seropositivity with age, while the seropositivity for N. caninum was associated with cattle age. The farm of cattle above the age of 5 years (16.8%) was more infective than less than 4 years (9.3%). This is the first report on seroprevalence of N. canium and T. gondii in Holstein and Korean indigenous cattle in Gyeongnam, Korea.
Prevalence of Theileria sergenti infection in cattle of eastern areas in Gyeongbuk province by PCR
Seo, Min-Goo ; Do, Jae-Cheu ; Cho, Min-Hee ; Seo, Hee-Jin ; Kim, Joong-Kew ; Kim, Young-Hoan ; Park, No-Chan ; Kwak, Dong-Mi ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 251~258
DOI : 10.7853/kjvs.2011.34.3.251
This study was performed to determine the prevalence of Theileria sergenti (T. sergenti) in cattle reared in eastern areas of Gyeongbuk province by PCR. Among 443 samples collected from 42 cattle farms, 96 (21.7%) samples from 13 (31.0%) farms were positive for T. sergenti. By regions, 87 (26.6%) out of 327 cattle in Gyeongju, 8 (9.5%) out of 84 cattle in Pohang, and 1 (3.1%) out of 32 cattle in Ulleung were positive for T. sergenti. T. sergenti infection in dairy cattle (27.9%) was significantly higher than that in Korean cattle (9.4%, P<0.0001). Accordingly, Korean cattle were more resistant to T. sergenti infection. Prevalence of T. sergenti in cattle was increased with age (P<0.0001). The infection rate in cows (23.3%) was significantly higher than that in bulls (5.0%, P<0.01). Seasonally, prevalence of T. sergenti in cattle was highest in autumn (32.7%, P<0.01). Prevalence of T. sergenti in grazing cattle (66.7%) was significantly higher than that in non-grazing cattle (15.8%, P<0.0001). Since prevalence of T. sergenti infection is high in cattle reared in eastern areas of Gyeongbuk province, survey on other hemoparasites and appropriate control programs need to be established in this region.
A survey on gastrointestinal parasites of Korean water deer (Hydropotes inermis agyropus) in Chungbuk province
Choe, Seong-Jun ; Na, Ki-Jeong ; Jee, Cha-Ho ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 259~263
DOI : 10.7853/kjvs.2011.34.3.259
Wildlife has been recognized that has important role as reservoir hosts of disease affecting both people and livestocks. However, information intestinal helminths of Korean water deer were limited. We obtained 20 feces from Korean water deers that were transferred to veterinary hospital in Chungbuk National University from May 2010 to June 2011. Parasite ova were collected using saturated zinc sulfate floatation method. Parasite eggs were examined and measured using light microscope. The identification of parasitic eggs was done by morphological characters. The overall infection rate of parasite was 85% (n=17). The eggs were observed as being Nematodirus spp. (n=14), strongyles egg (n=11), Capillaria spp. (n=9), Trichuris spp. (n=7), Eimeria spp. (n=4), Ogmocotyle spp. (n=3), and Strongyloides (n=1). Mixed infection rate was observed as being single 15% (n=3), double 20% (n=3), triple 15% (n=3), quadruple 30% (n=6), and quintuple 5% (n=1). The Ogmocotyle spp. was first reported in Korea.
Comparison of polymerase chain reaction for antigen receptor gene rearrangement and flow cytometric analysis for the diagnosis of canine lymphoma
Song, Ru-Hui ; Yu, Do-Hyeon ; Kim, Jun-Hwan ; Lee, Hyun-Seok ; Lee, Da-Mi ; Park, Chul ; Yu, Il-Jung ; Park, Jin-Ho ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 265~271
DOI : 10.7853/kjvs.2011.34.3.265
Lymphoma is the most common hematopoietic malignancy in dogs. Diagnosis of lymphoma is classically performed by morphological assessment and immunohistochemistry. But some cases in the early stage are difficult to distinguish and need more objective and accurate methods. So, Polymerase chain reaction (PCR) for antigen receptor rearrangements (PARR) and flow cytometric immunophenotype of lymphoma have been developed continuously. In this study, we performed these two methods to classify lymphoma type in 3 cases. According to PARR analysis, B cell origin lymphoma was diagnosed in two of three cases by testing PBMC and lymph node. All fine needle aspiration (FNA) samples of lymph nodes had high expression of CD21 on >88% of total cell population and PBMC samples also showed high expression of CD21 on >30% of total lymphocytes in those two cases, while the expression of CD3, CD4 and CD8 was absent. These results suggest that concurrent use of PARR and flow cytometric immunophenotype is more effective and valuable tool for the diagnosis and monitoring of canine lymphoma patients.
Diagnosis of immunohistochemical marker expressed by a canine Sertoli cell tumor case
Kim, Sung-Jae ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 273~278
DOI : 10.7853/kjvs.2011.34.3.273
Sertoli cell tumor (SCT) of the testicle arises from the supporting cells within the seminiferous tubules. SCT is common in dogs, especially in cryptorchid testicles, but also has been reported in the stallion, ram, cat, and bull. Sertoli cell tumor sample was collected from 7-years male german shepherd. In this study, SCT arose from one testicle. Sample size is approximately 1.7 cm in diameter and it has a round form. In the microscopic, cells within the tumor variably resemble Sertoli cells (SCs) that normally populate the seminiferous tubules and interstitial area. There is abundant stroma of dense, mature fibrous connective tissue in SCT. In the immunohistochemical staining, cytokeratin AE1/AE3 was not expressed in the control and SCT. S-100 protein was expressed by SCs, germ cells and fibrous connective tissue of SCT. Melan A was expressed by leydig cells (LCs) of SCT. A study by using S-100 and melan A in canine SCT was almost never carried out. S-100 and melans A is considered to suggest for diagnosis and pathogenesis of canine SCTs. Inhibin-alpha and Vimentin were well known as the marekers of SCTs. Also, they were expressed by Sertoli cells and LSs of SCT in this study.
Survey of the heavy metal contents in avian eggs from Korean markets
Kang, Kyoung-Sook ; Park, Hyoung-Sook ; Choi, Tae-Seok ; Shin, Bang-Woo ; Lee, Jeong-Hark ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 279~284
DOI : 10.7853/kjvs.2011.34.3.279
This study was carried out to estimate the heavy metal contents (Pb, Cd, Cr, Cu, Mn) in avian eggs sold in Seoul area, Korea from August to December, 2010. The contents of lead, cadmium, chrome, copper, manganese were estimated by inductively coupled plasma mass spectrometry. The concentrations of heavy metals (mean
standard deviation, mg/kg) in the avian eggs were as follows: Pb
. The contents of lead and cadmium in the eggs were lower than that in meat and other foods of previously reported results. Whereas the contents of copper, manganese were similar to the previously previous results. The average daily intakes of lead and cadmium in the avian eggs were 0.1 and 0.8% respectively, as compared with PTWI (Provisional Tolerable Weekly Intakes) established by FAO/WHO Expert Committee for Food Safety Evaluation.
Mornitoring of non-steroidal anti-inflammatory drugs in livestock products
Choi, Yoon-Hwa ; Kim, Yoen-Joo ; Shin, Bang-Woo ; Lee, Jung-Hark ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 285~289
DOI : 10.7853/kjvs.2011.34.3.285
This study was conducted to determine the content of non-steroidal anti-inflammatory drugs (NSAIDs) in meats available on the Korean markets. The analysis was carried out using following conditions; C18 column (
), mobile phase composed of DW (containing 0.1% formic acid): acetonitrile (containing 0.1% formic acid), binary pump at a flow rate of 0.3 ml/min and 5
of injection volume, MS/MS detector with ESI positive mode. The calibration range of five NSAIDs showed linearity (
) at concentration range of 3.125~200
/kg. The recoveries in fortified muscle more than 78.7~100.3%. The detection limits for meloxicam, ketoprofen, flunixin, carprofen and tolfenamic acid were 3.5, 1.6, 1.7, 9.8 and 4.8
/kg, respectively. We also monitored NSAIDs residue in cattle muscle 51 samples. The test results, NSAIDs were all not founded.
Study on chromosomes survey of Korea native dogs
Park, Chang-Eun ;
Korean Journal of Veterinary Service, volume 34, issue 3, 2011, Pages 291~296
DOI : 10.7853/kjvs.2011.34.3.291
The karyotype of the domestic dog is widely accepted as one of the difficult mammalian karyotypes to work. In contrast to many other animals, knowledge about the canine karyotype is quite sparse. The dog has a total of 78 chromosomes; all 76 autosomes are acrocentric in morphology and show only a gradual decrease in length. But appear to be quite small and difficult to identify unambiguously. To purchased standardization of chromosome in Korea native dog, there were analyzed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with 4, 6, 8, 11, 13, 17 chromosome. There were no variations in karyotypes which were analyzed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with Sapsaree, Jindo, Gyeongju DongGyeong dogs, Welshi-Corgi. It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation. Comparative analysis of the distribution patterns of conserved segments defined by dog paints in the genomes of the Korea native dogs demonstrates that their differences in the karyotypes of these three species could have resulted from acrocentric banding patterns.