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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 34, Issue 4 - Dec 2011
Volume 34, Issue 3 - Sep 2011
Volume 34, Issue 2 - Jun 2011
Volume 34, Issue 1 - Mar 2011
Selecting the target year
Serological survey of rabies virus from the stray dogs in Seoul
Kim, Neung-Hee ; Chae, Hee-Sun ; Son, Hong-Rak ; Kang, Young-Il ; Lee, Jung-Hark ; Kim, Se-Gon ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 297~301
DOI : 10.7853/kjvs.2011.34.4.297
Rabies virus is transmitted most commonly through a bite from an infected dog. Especially, stray dogs that are not excluded from contact with rabid wild animals can become rabies vectors. Therefore, serological survey of rabies virus from stray dogs in Seoul Metropolitan City was carried out in this study. To investigate prevalence of rabies antibodies in the stray dogs, serum samples were taken from 500 stray dogs between April and December 2010. Antibodies to rabies virus were detected by indirect ELISA. Of 500 tested sera, 147 (29.4%) were positive to rabies virus. Prevalence rates of rabies antibodies(PRRA) in northern and southern Han river region of Seoul were 26.4% and 33.2% respectively. PRRA in male and female dogs were 33.6% and 26.1% respectively. PRRA in less than 1 year, 1~2< years, 2~3< years, 3~5< years, 5~10< years and over 10 years old dogs were 12.7%, 21.6%, 26.4%, 36.4%, 32.5% and 46.4%, respectively. These results indicates that antibody seroprevalence to rabies is still not enough to prevent rabies and rabies vaccination is required to enhance the seroprevalence in the dogs. To improve the situation, measures to raise public awareness of rabies and its prevention is needed. Also, reducing stray dogs and keeping companion animals from contact with wild animals are indispensable for the prevention of rabies.
Prevalence of infectious agents in cattle reared in Ulleung island
Seo, Min-Goo ; Do, Jae-Cheul ; Ouh, In-Ohk ; Coh, Min-Hee ; Kim, Joong-Kew ; Kim, Young-Hoan ; Park, No-Chan ; Kwak, Dong-Mi ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 303~311
DOI : 10.7853/kjvs.2011.34.4.303
Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007~2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008~2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007~2010. For BLV and MP, no sample was positive by ELISA in 2007~2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008~2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.
Study on gross finding of lung lesions and causative pathogens of porcine respiratory disease complex from slaughtered pigs in Incheon
Lee, Chang-Hee ; Hwang, Weon-Moo ; Lee, Jung-Goo ; Lee, Sung-Mo ; Kim, Sung-Jae ; Kim, Nam-Hee ; Yang, Don-Sik ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 313~320
DOI : 10.7853/kjvs.2011.34.4.313
The purpose of this study was to investigate association with gross lesions and causative pathogens of porcine respiratory disease complex (PRDC) including porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (MH), Pasteurella multocida (PM), Actinobacillus pleuropneumoniae (APP), Haemophilus parasuis (HP) in slaughtered pigs. A total of 1,200 lung samples were collected randomly from slaughtered pigs in Korea during August of 2010 through July of 2011. The gross lesions were classified according to the six stages (0, 1~10, 11~20, 21~30, 31~40 and
, unit=%) and 48 samples from each stage were selected to detect viral and bacterial pathogens. The results according to the six stages were 100 (8.3%), 259 (21.6%), 326 (27.2%), 213 (17.8%), 144 (12.0%) and 158 (13.2%) cases, respectively. Prevalence of pneumonia according to season was 87.0~96.7% and the highest prevalence was in spring. In detection of pathogens by PCR, 53 samples were not detected any causative pathogens of PRDC. PCV2, PRRSV, SIV, MH, PM, APP serotype 2, APP serotype 5 and HP were positive in 45.5%, 12.5%, 10.4%, 60.1%, 1.7%, 13.9%, 12.2% and 15.6%, respectively. In co-infection, PCV2-MH was the most detected causative pathogens of PRDC. The detection rate of PCV2 and PRRSV was the highest in spring, of SIV, MH and HP was in winter. The detection rate of APP-2 and APP-5 had no seasonal prevalence. The more severe gross lesions increased, the higher the detection rate showed.
Development of real-time PCR for rapid detection of Mycobacterium bovis DNA in cattle lymph nodes and differentiation of M. bovis and M. tuberculosis
Koh, Ba-Ra-Da ; Jang, Young-Boo ; Ku, Bok-Kyung ; Cho, Ho-Seong ; Bae, Seong-Yeol ; Na, Ho-Myung ; Park, Seong-Do ; Kim, Yong-Hwan ; Mun, Yong-Un ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 321~331
DOI : 10.7853/kjvs.2011.34.4.321
Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical-based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.
Development of loop-mediated isothermal amplification method for the rapid and sensitive detection of bovine tuberculosis in Korea native cattle
Hwang, Eun-Suk ; Lee, Tae-Uk ; Jung, Dae-Young ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 333~339
DOI : 10.7853/kjvs.2011.34.4.333
Loop-mediated isothermal amplification (LAMP) was developed to detect Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacterium (NTM) genomic DNA in blood samples of Korea native cattle. A set of four primers, two outer and two inner, were designed from M. bovis and M. avium genomic DNA targeting the IS6110 and 16S rRNA gene, respectively. Based on 85 Intradermal Tuberculin Test (ITT) positive blood sample and using conventional PCR and LAMP, the agreement quotient (kappa), which measures agreement beyond chance were 0.93 (conventional PCR) and 0.97 (LAMP), respectively. The detection limit of the LAMP method was
copy/ml M. bovis and M. avium cells, compared to
copy/ml M. bovis and M. avium cells for conventional PCR. These results suggest that the LAMP is a powerful tool for rapid, sensitive, and practical detection of MTC and NTM in blood samples of Korea native cattle.
Evaluation of bacteriophages for prevention and treatment of diarrhea due to experimental enterotoxigenic Escherichia coli K88 infection of weaned piglets
Kim, Sung-Jae ; Kim, Jin-Dong ; Yang, Si-Yong ; Kim, Nam-Hee ; Lee, Chang-Hee ; Yang, Don-Sik ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 341~352
DOI : 10.7853/kjvs.2011.34.4.341
Colibacillosis in pigs remain a major swine industry bruden worldwide. Although some progress has been made in treating collibacillosis in pigs by using biosecurity and antimicrobials, it still remain a considerable problem. The use of host-specific bateriophages as a biocontrol is one possible alternative. The purpose of this study was to evaluate the effect of bacteriophage against enterotoxigenic Escherichia coli (ETEC) K88 infection in piglets. Twenty-eight piglets were randomly divided into four groups and each group was allocated with 7 pigs. Group B, C and D were inoculated with 5 ml of ETEC K88 (
CFU/ml) per head of piglet via oral. Group C and D were fed with bacteriophages (Group C,
PFU/g; Group D,
PFU/g; CJ CheilJedang Corp., Korea) orally as treatment. In piglets administrated bacteriophages and challenged with ETEC K88 (Group C and D), Clinical signs and the growth performance were improved and antibody titers were maintained low level compared with piglets challenged with ETEC K88 (Group B, P<0.05). Group B were shown high pH in the alimentary tracts compared with other piglets (P<0.05). In quantitative analysis by real-time PCR, the results of Group C and D were lower than those Group B in faecal and intestinal samples (P<0.05). Severe villus atrophy and crypt hyperplasia were observed in Group B consequently V/C ratio increased, compared with other piglets. These results indicate that feeding with bacteriophage has effect to prevent ETEC K88 infection in piglets and suggest that use of bacteriophage can be considered a valid antibiotic alternative.
An outbreak of chronic fowl cholera in broiler breeder chickens in Korea
Kim, Jin-Hyun ; Yoon, Mi-Young ; Cho, Jae-Keun ; Sung, Myung-Suk ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 353~359
DOI : 10.7853/kjvs.2011.34.4.353
Fowl cholera is a contagious acute and chronic disease caused by Pasteurella multocida in both domesticated and wild birds. Acute fowl cholera in both chickens and wild birds has recently been documented in Korea, but the chronic form has not been reported in Korea until now. This study describes the first outbreak of chronic fowl cholera in 13-week-old Arbor Acre broiler breeder chickens submitted to the College of Veterinary Medicine, Kyungpook National University in April 2006. The clinical signs of the affected flock of 9,621 chickens were lameness caused by swollen hock joints, diarrhea, ruffled feathers, and an average weekly mortality of 1.0%. At necropsy, purulent or caseous exudates were found in the hock and wing joints, humerus, and eyes, and severe pneumonia and pericarditis were discovered. Eleven bacterial isolates obtained from the liver, joint, infraorbital sinus and sternal bursa of the submitted chickens were all identified as Pasteurella multocida based on their physiological and biochemical characteristics. Five isolates were examined for antimicrobial susceptibility against 21 different antimicrobial agents including ampicillin. All were resistant to kanamycin, neomycin, and streptomycin, and some were resistant to gentamicin. The tested isolates were all susceptible to the other 17 antimicrobial agents. All 11 isolates were capsular serogroup A based on multiplex polymerase chain reaction. In addition, two of five isolates used in the antimicrobial susceptibility test were identified as somatic serotype 1 by an agar gel diffusion precipitin test, while the others were non-typable.
Isolation and antimicrobial susceptibility of microorganisms from milk samples of healthy postpartum sows
Kim, Seong-Tae ; Kim, Seon-Deuk ; Park, Jun-Young ; Cho, In-Young ; Choi, Jong-Song ; Jung, Ji-Young ; Lee, Ju-Dan ; Hur, Tai-Young ; Jung, Young-Hun ; Choi, Chang-Yong ; Shin, Sung-Shik ; Son, Chang-Ho ; Ok, Ki-Seok ; Suh, Guk-Hyun ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 361~368
DOI : 10.7853/kjvs.2011.34.4.361
The growth rate of piglets will be decreased and the mortality of piglets will be increased in the postpartum sow with mastitis. The aim of this study was to isolate microorganisms from milk samples found in postpartum sows with suckling piglets and to further investigate antimicrobial susceptibility against isolated bacteria. Milk samples were collected from 567 udders of 42 lactating sows and the isolated bacteria was then identified. Isolated bacteria were tested with 12 antimicrobial agents. Bacteria were isolated from 260 milk samples (64.5%). Staphylococcus spp. were the most common microorganisms (74.5%) isolated from sow milk, followed by Streptococcus hyicus (53.9%), Staphylococcus epidermis (11.8%), Staphylococcus aureus (9.8%), and Staphylococcus haemolyticus (53.9%). In the Staphylococcus spp., Streptococcus pneumoniae (5.1%) was isolated as was Streptococcus sanguinis (3.8%) and Streptococcus ovis (2.9%). Results indicated that bacteria isolated from healthy sow milk were mostly susceptible to cephalothin (88.0%), amikacin (85.3%) and cefoxitin (82.4%), but were resistant to streptomycin (10.0%), kanamycin (33.1%) and tetracycline (19.6%).
A retrospective study of the incidence of clinical mastitis found in large-scale dairy herds in Korea
Kang, Ju-Hoe ; Kim, Seon-Deuk ; Park, Jun-Young ; Cho, In-Young ; Hur, Tai-Young ; Jung, Young-Hun ; Choi, Chang-Yong ; Jung, Ji-Young ; Shin, Sung-Shik ; Son, Chang-Ho ; Ok, Ki-Seok ; Suh, Guk-Hyun ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 369~378
DOI : 10.7853/kjvs.2011.34.4.369
Incidence of mastitis is highest in dairy cows and it is a leader of all diseases and the economic losses, in dairy farms. The objective of this study is to analyse the incidence of clinical mastitis using the lactation number, lactation month and season of calving. In this study, we used 3,779 lactation data and animal health records collected from 1990 to 2006 at the National Institute of Animal Science. Out of 3,779 cows, a total of 1,721 cows were reported with clinical mastitis, which was 46.3%. The frequency of mastitis increased from 36.9% from first lactation to 56.0% by the fifth lactation. As many as 766 cows (46.9%) showed a recurrence of mastitis after 14 days, apart for two or more and chronic mastitis that were recorded for 657 cows (20.3%). This came to a total of 3,010 cases that had clinical mastitis in the herd for sixteen years. The distribution of incidence of clinical mastitis was highest during the first month of lactation at 24.4%, and it reached to 43.4% by the third month. The incidence of mastitis was even higher for cows in first lactation, showing 28.6% in the first month, and 42.9 percent by third month of lactation. Cows calved during the summer and winter months showed higher rate of incidence with 59.9% and 57.9% within 30 days of lactation, respectively. It is urgently needed to establish a preventative management for heifer cows during their expected first lactation and the clinical specific dry-period management strategies that influence the rate of clinical mastitis during the next lactation. The aim of this study is to present information that might be useful to improve clinical mastitis prevention.
Survey of mastitis management and incidence of mastitis in high somatic cell count of bulk milk at dairy farms in the Gyeongnam
Kim, Seong-Eun ; Hah, Do-Yun ; Jang, Eun-Hee ; Kwon, Hee-Nyung ; Jo, Seong-Suk ; Kwon, Young-Taek ; Park, Dong-Yeop ; Lee, Kuk-Cheon ; Kim, Jong-Shu ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 379~388
DOI : 10.7853/kjvs.2011.34.4.379
Survey of mastitis management and incidence of mastitis in the Gyeongnam was started in May to September 2009 to solve mastitis problem statistically valid data for use in estimating mastitis management, isolation and antimicrobial drug susceptibility in 30 dairy farms having over 350,000/ml somatic cell count. In investigation on recognition of farmer about bovine mastitis, the ratio of understanding of differences between infectious and environmental origin, understanding of correlation between superbacteria and using indiscriminate, necessity of pathogen identification, and necessity of antimicrobial sensitivity tests were 80.0%, 73.3%, 33.3%, and 53.3%, respectively. In survey of mastitis management type, regular california mastitis test (CMT), conducting CMT test and empirical self-treatment, when detecting suspected cows, were 30.0%, 40.0%, and 46.7%, respectively. Checking and cleaning pulsators biweekly, cleaning vacuum system and replacing liners every 3~6 month, and getting milking system checked by engineers showed 80.0%, 76.7%, and 76.7% in the questionnaires, respectively. In recognition of farmer about milking hygiene for prevention of bovine mastitis, using individual towels, separated milking (milking order of cows), and teat-dipping disinfection after milking exhibited 13.3%, 86.7%, and 93.3%, respectively. In conclusion, through the questionnaires and laboratory test, we suggest that recognition of farmer about management and incidence of mastitis was very low, thus systemic educational program and public relations about mastitis management were need for dairy farmers.
Pathological observation of porcine respiratory disease in slaughter pigs
Kim, Nam-Hee ; Hwang, Weon-Moo ; Lee, Jung-Goo ; Lee, Sung-Mo ; Yang, Don-Sik ; Lee, Chang-Hee ; Kim, Sung-Jae ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 389~395
DOI : 10.7853/kjvs.2011.34.4.389
The aims of this study were to assess the lung lesions and to compare gross observations with histopathological findings in the lungs of slaughter pigs. During August of 2010 through July of 2011, the 1,200 lung samples were randomly collected from slaughtered pigs in Korea. We examined prevalence of lung lesions and classified the lung lesion according to the severity of lung lesions. For the comparison of gross lesions with histopathological findings, BALT hyperplasia was also examined. Among the tested samples, 1,100 (91.7%) had pneumonic lung lesions. In the seasonal prevalence of pneumonic lung lesions, spring was the highest followed by winter, summer and autumn, respectively. The mean pneumonic score (
and the highest gross lesion according to stages was 11~20% (27.2%, 326/1,200). The prevalence of pleuritis was 7.0% and the highest prevalence was determined in summer. In severity, the lungs collected in this study were frequently in chronic stage (36.4%, 437/1,200), and it was shown that BALT hyperplasia was more extensive in chronic lesion. In this study, the prevalence of pneumonic lung lesions showed a high side and appeared particularly high in the spring. Severity of lung lesion was the most prevalent chronic lesions which were consistent with histopathologic findings.
Application of alkaline phosphatase staining of cytology specimen for differential diagnosis of canine osteosarcoma
Park, Byoung-Yong ; Park, Chul ; Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 397~401
DOI : 10.7853/kjvs.2011.34.4.397
Aspiration of lytic bone lesions is an excellent diagnostic test in the initial evaluation of primary bone tumor. However, cytologically, it can be difficult to differentiate osteosarcoma (OSA) from other bone neoplasms, including fibrosarcoma, chondrosarcoma, synovial cell sarcoma, malignant fibrous histiocytoma and malignant peripheral nerve sheath tumor. The purpose of this study is to introduce alkaline phosphatase (ALP) staining to differentiate OSA from other mesenchymal tumors. Tumors actively producing bone are specifically positive for ALP staining. Unstained, cytologic specimens were incubated for 10 minutes with nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate toluidine salt-phosphatase substrate. Among 20 cases of cytology specimen, 14 were positive for ALP staining and histopathology, 6 were negative for ALP staining and histopathology. ALP staining was 100% sensitive and specificity for the diagnosis of OSA. Aspirate cytology with ALP staining was a simple, fast, safe and accurate diagnostic test for the evaluation of suspected OSA lesions in dogs.
Spontaneous canine T-cell lymphosarcoma in a Shih Tzu dog
Oh, Hong-Guen ; Chung, Yung-Ho ; Lee, Hyun-A ; Hong, Sun-Hwa ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 403~407
DOI : 10.7853/kjvs.2011.34.4.403
This study describes a canine lymphosarcoma with a rapid proliferation and recurrence. A 4-year-old, male, Shih Tzu dog was examined for acute swelling mass. The mass had been identified since 3 months ago and enlarged
cm and located in the right axillary region. The surgical removal was recommended when patient visited veterinarian and the operation was conducted. The removed tumor was
cm and firm, lobulated and white cut surface. Routine screening laboratory test was assessed with blood and radiological analysis. The metastasis sign was not detected on thoracic and abdominal radiography. Blood test revealed decreased lymphocytes. After surgical removal of the mass, microscopic histopathological examination was performed to determine the final diagnosis. Histopathologically, the tumors are characterized by the same histological features, including the presence of neoplastic cellular populations, and lymphocytes infiltration in varying proportions. Also, DNA was extracted and PCR analysis was employed to analyze the origin of tumor cells. T-cell specific nucleic acid fragments were specifically amplified by PCR. On the basis of the laboratory results, the tumor was diagnosed with canine T-cell lymphosarcoma. On the basis of our knowledge, this is the first report of canine T-cell lymphosarcoma in a Shih Tzu dog.
Evaluation of the microbial contamination status and sanitation practice level in butcher's shops in Seoul
Jeon, Hae-Chang ; Kim, Jee-Eun ; Son, Jang-Won ; Chae, Hee-Sun ; Jin, Kyung-Sun ; Oh, Jae-Hyo ; Shin, Bang-Woo ; Lee, Jung-Hark ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 409~416
DOI : 10.7853/kjvs.2011.34.4.409
This study was performed to evaluate the microbial contamination level of meat processing in butcher's shops in Seoul from January to November in 2010. A total of 695 samples (112 cotton work gloves, 342 utensils and equipments, 241 meat samples) were collected and the environmental hygiene of 89 butcher's shops was investigated. The aerobic plate count (APC), E. coli and pathogenic bacteria such as Staphylococcus aureus, Salmonella spp. were tested in the samples. As a result, the level of count on APC ranged
from the utensils & equipments,
CFU/glove from the cotton work gloves and
CFU/g from the meat samples. The level of count on E. coli ranged
from the utensils,
CFU/glove from the cotton work gloves and
CFU/g from the meat samples, respectively. Staphylococcus aureus and Salmonella spp. were detected in 11 samples and 2 samples from the cotton work gloves, utensils & equipments, respectively. The sanitation standard operating procedure (SSOP) was applicated at 49 butcher's shops. In order to improve sanitation of meat in butcher's shops, in this study, applications of SSOP, systemic sanitation education for employees, hygenic control of utensils & equipments, and continuous monitoring for microorganisms will be required.
Development of species-specific multiplex PCR assays of mitochondrial 12S rRNA and 16S rRNA for the identification of animal species
Koh, Ba-Ra-Da ; Kim, Ji-Yeon ; Na, Ho-Myung ; Park, Seong-Do ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 417~428
DOI : 10.7853/kjvs.2011.34.4.417
Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated (
for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.
Comparison between the liquid-liquid partition method and modified QuEChERS method for the analysis of pesticide residues in beef fat
Kim, Yoen-Joo ; Choi, Yoon-Hwa ; Shin, Bang-Woo ; Lee, Jung-Hark ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 429~439
DOI : 10.7853/kjvs.2011.34.4.429
This article described the comparison of a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation and the classical method established by National Veterinary Research and Quarantine Service (NVRQS) for the determination of pesticide residues in livestock products using GC-tandem mass spectrometry. The classical method by NVRQS used liquid-liquid partioning followed by evaporizing. The modified QuEChERS entailed extraction of 2 g sample with 15 ml acetonitrile containing 1% acetic acid followed by addition of 6 g anhydrous magnesium sulfate and 1.5 g sodium acetate. After centrifugation, 6 ml of the extract underwent a cleanup step (in a technique known as column-based solid phase extraction) using 400 mg each of
and primary secondary amine sorbents plus 1,200 mg magnesium sulfate. The quantitation of individual pesticides by both methods was based on tissue standard calibration curves with a correlation coefficient in excess of 0.98 for the 24 pesticides. The detection limits by the classical method were ranged 1.3~5.0
/kg, with mean recoveries between 76.2% and 114.3% except aldrin (59.3%) and deltamethrin (63.6%). The detection limits by modified QuEChERS were ranged 0.3~6.2
/kg, with mean recoveries between 68.0% and 114.3% except dimethipin (152.6%), chlorfenvinphos (138.1%), 4,4-DDT (61.5%), aldrin (60.4%) and chinomethionate (30.3%).
Suggestions for a better HACCP system assessment in livestock product processing plants
Hong, Chong-Hae ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 34, issue 4, 2011, Pages 441~448
DOI : 10.7853/kjvs.2011.34.4.441
According to the Korean government policy of expanding HACCP system application to food chain from farm to meat market, the importance of HACCP assessment to keep balance among the certified HACCP plants is urged. The objective of this study is to recommend more effective HACCP assessment methods. We reviewed major foreign countries HACCP implementation policy focused on the audit system, and compared with the Korean HACCP assessment system. We found that the checklist guidelines of prerequisite program were not enough for precise evaluation and many of the items were not directly related to the risk occurrences. And current rating grade for each items and judgments were inadequate to induce non-compliances and corrections for better HACCP implementation. We suggest revision points as follows; (1) checklists should be revised and reorganized according to the possibility of risk occurrences, (2) all the items should be supported by detailed guidelines for more objective inspection, (3) non-compliances identified must be reconfirmed after correction, (4) the items for HACCP plan should be divided into an accreditation inspection and a regular inspection, (5) rating grade 'high', 'medium', 'low', 'failure' was better for indicating non-compliances.