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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 35, Issue 4 - Dec 2012
Volume 35, Issue 3 - Sep 2012
Volume 35, Issue 2 - Jun 2012
Volume 35, Issue 1 - Mar 2012
Selecting the target year
Investigation on antimicrobial resistance genes of Salmonella Schwarzengrund isolated from pigs
Lee, Woo-Won ; Kim, Sang-Hyun ; Lee, Seung-Mi ; Lee, Gang-Rok ; Lee, Gi-Heun ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 1~8
DOI : 10.7853/kjvs.2012.35.1.001
To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (
), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.
Isolation and identification of infectious bursal disease virus from broiler and layer chickens during the outbreak year 2007 in Bangladesh
Islam, Md. Taohidul ; Mohiuddin, Mohammad ; Hossain, Muhammad Tofazzal ; Rahman, Md. Bahanur ; Rahman, Md. Mostafizur ; Rahman, Md. Siddiqur ; Song, Hee-Jong ; Islam, Md. Alimul ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 9~17
DOI : 10.7853/kjvs.2012.35.1.009
The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.
Investigation of Toxoplasma gondii infection on stray cats in Daejeon
Sung, Sun-Hye ; Yoo, Sang-Sik ; Im, Yeo-Jeong ; Chung, Nyun-Ki ; Moon, Byung-Chun ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 19~24
DOI : 10.7853/kjvs.2012.35.1.019
This study was performed to evaluate the prevalence rate of Toxoplasma gondii on 217 stray cats in Daejeon. The positive infection rate of T. gondii was 15.7% in enzyme-linked immunosorbent assay (ELISA), 12.4% in latex agglutination test (LAT), 14.7% in indirect immunofluorescent antibody test (IFA) and 0.5% in polymerase chain reaction (PCR) respectively. In districts, Yuseong-gu was shown the highest seropositive rate of T. gondii as 31.8% in ELISA, 22.7% in LAT and 31.8% in IFA. In gender, the seropositive rate of female cats was slightly higher than that of male cats as 17.2% in ELISA, 15.2% in LAT, 15.2% in IFA and 1.0% in PCR. Cats captured in National science museum, detached house and apartment was shown relatively high prevalence rate of T. gondii.
Inhibitory effects of herbal medicine on coccidiosis
Park, Young-Suk ; Kim, Jeong-Rak ; Kang, Byeong-Kwon ; Choe, Yeong-Ho ; Kim, Byeong-Soo ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 25~31
DOI : 10.7853/kjvs.2012.35.1.025
This study examined the efficacy of natural ingredient formulated herbal medicine as feed additives against coccidiosis in broiler chicken. Medicinal herbs used in this study were Artemisia annua, Pulsatilla chinensis, Clematis chinensis and Scutellaria baicalensis. Two week old chicks were administered with 1% ingredient formulated herbal medicine. Observations of the efficacy were confirmed by examining the lesion score, histopathological appearance of cecum, shedding of fecal oocyst and production index in farm. S. baicalensis of the most effective substance were analyzed by UPLC. The results are as follows; among various types of Chinese medicine, the most efficacious ingredient was found to be pure bicalein. The major index substance is bicalein and
per gram was found. Feeding of chickens with S. baicalensis induced the coccidiostat effect and reduced the fecal shedding of E. tenella oocyst by 14%. Experimental results on a farm, feed conversion ratio and weight gain and production rate in the group with natural ingredient formulated additives than in control group increased. The rate of weight gain (%) in each experimental group showed 108% and 126% increase while the mortality rate was 1.4% lower than the control group. The investigation showed that the most efficacious ingredient was found to be S. baicalensis among herbal medicine products for coccidiostats. However, as it was found in the farm experiment that pure S. baicalensis alone is economically unviable. The additives with cocktailed of efficacious ingredient are suggested to be implemented for effective protection against coccidiosis.
Analysis of the wildlife distress and rescue of wild animal mammals in Gangwon province
Park, In-Chul ; Lee, Ho-Won ; Kim, Jong-Taek ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 33~37
DOI : 10.7853/kjvs.2012.35.1.033
As people's interest about wildlife animal increases, more wildlife animal centers are developing. In South Korea, currently 120 mammal species live, and 22 of them are designated as endangered species. It is important to protect these natural treasures and endangered species, because they are being harmed by human industrial activity. For example, road expansion and poaching have led to the destruction of wildlife animal habitats, which is increasing year by year. Nevertheless, data regarding the systematic treatment of wild animals that are in distress is lacking, and it is a reality that analysis of such data is also lacking. The Kangwon Wildlife Rescue Center investigates these types of distress experienced by mammals. By analyzing the shape of the wild animals killed or injured, the center seeks an efficient and systematic way to manage such distress. The data collected from the Wildlife Animal Center from September 2007 to August 2010 includes 195 different cases, and the animals point of rescue. There are 16 species, including 87 water deer (Hydropotes inermis), 39 raccoons (Nyctereutes procyonoides), 20 roe deer (Capreolus pygargus bedfordi), 10 goral (Nemorhaedus goral), 7 siberian weasels (Mustela sibirica), 6 otters (Lutra lutra) and etc. Within the first year (September 2007 to August 2008), the number of mammals rescued was 46. In the second year (September 2008 to August 2009), 60 animals were rescued. in the third year (September 2009 to August 2010), the Wildlife Animal Center had 89 animals. We can see that these numbers are increasing, which shows that people are interested in the well-being of these animals because they are reporting animal injuries more and more. The mains form of distress caused by humans are from car accidents 67 (34.4%). Traps, and other hunting tools used by poachers are responsible for 25 (13.0%). Also, human industrial activity thus indirectly led to the starvation 25 (13.0%). and the orphaning of young animals 37 (19.0%).
Analysis of the wildlife distress and rescue of wild avian animals in Gangwon province
Park, In-Chul ; Kim, Ji-Won ; Kim, Jong-Taek ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 39~45
DOI : 10.7853/kjvs.2012.35.1.039
This survey aims to provide information on the pattern of distress in wild avian animals in Gangwon province. This survey is based on 315 wild avian which were rescued by the Kangwon University Wildlife Rescue Center. The data was collected at the Wildlife Animal Center from September 2007 to August 2010, and by analyzing the shape of the wild animals killed or injured, the center seeks an efficient and systematic way to manage such distress. There are 55 species found, in this study including 40 common kestrel, 34 eurasian eagles, 20 common buzzards, 20 collared scops owls, 19 oriental scops owls, 16 brown hawk owls and 160 others. These are the results of the survey: 129 (40.3%) of 315 avian birds were found between June and August. Many cases in that term were observed falling from the nest or losing their mother because of unskilled flight during the breeding season's flight practicing process. Between November and December, 64 individual (20.3%) were found, and it's the second largest number. The main reasons for these results were starvation and exhaustion from the winter migratory season. The results indicated that the main reason for avian distress came from the following: collision with architecture windows in 73 cases (23.2%), starvation and exhaustion in 63 cases (20.0%), collision with vehicles in 51 cases (16.2%), becoming orphaned in 69 cases (21.9%), trespassing into buildings occurred in 19 cases (6.0%), collision with electric wires in 18 cases (5.7%), poaching materials in 13 cases (4.1%), becoming entangled by fishing wire and other miscellaneous materials in 4 cases (1.3%), infection from parasites in 4 cases (1.3%), toxicosis from such toxins like agricultural chemicals in 1 case (0.3%). Overall, the purpose of this investigation was to analyze cases of avian distress in Korea's wildlife. This study used areas, seasons and causes to understand origins of these animals distress. So that it can be utilized for organized wild avian rescue and treatment.
Escherichia coli septicemia concurrent with mycotic infection in captive salt water crocodiles in Bangladesh
Sultana, Sajeda ; Chowdhury, Emdadul H. ; Parvin, R. ; Saha, Shib S. ; Rahman, Sheik M. ; Haider, M.G. ; Arif, Abu S.M. ; Rahman, Md. Siddiqur ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 47~52
DOI : 10.7853/kjvs.2012.35.1.047
Crocodile farms are getting popular in Bangladesh in an economic point of view. In one of the farms, some crocodiles were found sick and three of them died between May and July in 2006. This investigation was performed to diagnose the cause of the death. Routine postmortem examination was conducted. Samples were collected in 10% neutral buffered formalin for histopathology and in falcon tube for microbiological study. Additional swabs were collected in nutrient broth. Histopathological and microbiological studies were conducted using routine procedures. In addition Giemsa, Gram and PAS stains were performed to detect the organism in tissues. Grossly, esophagus, trachea, lungs, liver, spleen, heart and kidney were congested. Intestine, rectum and colon were hemorrhagic. Clay colored material was found in colo-rectum. Purulent exudates in lungs and thick and cloudy pericardial fluid in pericardial sac were found. Histologically, multifocal granulomatous inflammation was evident in lung, liver, kidney, intestine and colon with bacterial colonies, fungal spores and hyphae. These bacteria were appeared as Gram negative. Fungal hyphae and spores were detected in liver, lungs and colon by using PAS stain. Bacteriologically, E. coli were isolated from lungs exudates, pericardial fluids and intestinal fluids. Therefore, it can be concluded that 3 crocodiles died due to E. coli septicemia concurrent with mycotic infection.
A case of rape poisoning in a Hanwoo
Do, Jae-Cheul ; Kim, Eun-Young ; Kim, In-Kyoung ; Cho, Min-Hee ; Kim, Joong-Kew ; Park, No-Chan ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 53~57
DOI : 10.7853/kjvs.2012.35.1.053
This is a case report about rape poisoning in a Hanwoo. Non-protein nitrogen (NPN) compound in rape may cause poisoning in domestic animals. Rape poisoned cattle may show big and rapid breath with opening its mouth, edema and erosion of skin with itching, drooling, dyspnea and indigestion. Besides there can be shown neurological (blindness) and urological (red water) symptoms in some cases. There is no effective therapy for rape poisoning, therefore stopping the rape supplying is basically important. Clinical symptoms and history taking are very important factors in diagnosis of rape poisoning, due to the absence of diagnostic standard of judgment for rape poisoning. A Hanwoo 38 months old cow, in a farm located in Gyeoungbuk province showed typical rape poisoning symptoms such as breath with opening its mouth, drooling, dyspnea and hyperphotosensitivity, and dramatical recovery were occurred after stopping supplying of rape. The cow were diagnosed as the rape poisoning based on observation of clinical symptoms and history taking.
Salt poisoning in a Jeju native black sow
Yang, Hyoung-Seok ; Kang, Sang-Chul ; Kim, Jae-Beum ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 59~62
DOI : 10.7853/kjvs.2012.35.1.059
A 3-year-old Jeju native black sow with sudden death was requested to the Jeju Self-Governing Provincial Veterinary Research Institute for diagnosis in July 2009. Because the pig died suddenly, we could not find any clinical signs. Gross lesions such as pulmonary edema, redness of heart, liver, kidney and lymph nodes were observed at necropsy. Histopathologically, there were severe eosinophilic meningoencephalitis, atrophy, degeneration and necrosis of laminar neurons, and neovascularization in cerebral cortex. Based on histopathological findings and history taking about temporal suspension of water supply, this case was diagnosed as salt poisoning of a Jeju native sow. In our best knowledge, this is the first report for salt poisoning of sow in Korea, especially in Jeju native pig.
Spontaneous pyometra induced by Staphylococcus aureus in a Maltese
Hong, Sun-Hwa ; Chung, Yung-Ho ; Han, Sang-Jun ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 63~67
DOI : 10.7853/kjvs.2012.35.1.063
An 8-months-old young Maltese bitch was presented for investigation of anorexia and occasional vomiting. The body weight was 2 kg. Abdominal sonography demonstrated abnormal enlargement of uterus. The dog had been clinically and pathologically examined. Also, ovariohystectomy was performed. Staphylococcus aureus was identified in the pus-like materials of the uterine lumen. This case was confirmed as pyometra induced by Staphylococcus aureus. Gross examination of the dissected uterus revealed enlarged uterine horn, which had yellowish or brownish pus-like materials in the lumen. Also, there was several segments formation between the enlarged areas of left and right uterine horns. Histopathologically, endometritis and necrosis were present in uterine wall. The bitch made a complete recovery following an ovariohystectomy. This case was spontaneous pyometra induced by Staphylococcus species. On our knowledge, this is a very rare report of pyometra occurrence in young dog and Staphylococcus species-induced pyometra. This study may provide a valuable data on the study of canine pyometra.
A case of nonserotypable Escherichia coli infection in a Korean rabbit farm
Camer, Gerry A. ; Roh, Yoon-Seok ; Cho, A-Ra ; Kim, Jong-Won ; Umanets, Alexander ; Kim, Bum-Seok ; Lim, Suk-Kyung ; Lee, Hee-Soo ; Lim, Chae-Woong ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 69~71
DOI : 10.7853/kjvs.2012.35.1.069
Rabbits are highly susceptible to colibacillosis, and no treatment is able to control the disease effectively. Rabbits raised in a farm in Chonbuk province presenting persistent diarrhea and death were submitted for diagnosis. Ninety percent of the infected animals died; weanlings suffered the most mortality. Necropsies showed prominent hemorrhagic foci along the intestinal and cecal serosae. In histopathological examination, rod-shaped bacteria were observed in the necrotic areas of the tips of villi in the small intestine and neutrophils infiltration was found around the necrotic villous areas. The affected animals consistently yielded Escherichia coli isolates from the intestines. The isolated organism was atypically indole-negative and was nonserotypable using 62 known O group-typing sera. Further microbiological and epidemiological works to recognize and control colibacillosis infection in farmed rabbits in Korea is therefore critical.
Pulmonary aspergillosis in a slaty-backed gull ( Larus schistisagus )
Yoon, Jeong-Sik ; Kang, Sang-Chul ; Yun, Young-Min ; Lee, Sang ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 73~76
DOI : 10.7853/kjvs.2012.35.1.073
A female slaty-backed gull (Larus schistisagus) died suddenly without apparent clinical signs. At necropsy, well demarcated 7 to 10 mm yellow to white nodules were presented at the lungs and thoracic cavity. Microscopically, multifocal necrotic granulomas were observed in the lung tissue and amorphous acidophilic fibrin were accumulated in the granuloma and normal alveolar space. Periodic acid-Schiff (PAS) staining demonstrated numerous pinkish red branched hyphae embedded in the center of these granulomas. According to fungal culture using Sabouraud's dextrose agar plate, Aspergillus fumigatus was isolated from lung lesions. This is the first report for pulmonary aspergillosis of wild slaty-backed gull in Korea.
A primo vessel-like structure in a dog with inflammatory pseudotumor
Cho, Sung-Jin ; Hong, Sun-Hwa ; Han, Sang-Jun ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 35, issue 1, 2012, Pages 77~82
DOI : 10.7853/kjvs.2012.35.1.077
Inflammatory pseudotumor (IPT) is a term defining a mass characterized microscopically by a proliferation of bland mesenchymal spindle cells infiltrated by diffuse mixed inflammatory cells with a predominance of plasma cells and lymphocytes. Here, we show the primo vessel-like structure of the primo-vascular system (PVS) in a dog with IPT. A 6-years old male Mongrel dog was diagnosed with an abnormal mass (diameter 5.5 cm, weight 22 g) near left preputial area. The dog was submitted to the surgical detectomy of the mass. During the surgical operation, we observed primo vessel-like material. After fixations, the masses appeared macroscopically as lipoid-like, firm, white to grey masses, measuring
. Histologically, cellular infiltration into the muscular layers was frequently seen. The mesenchymal proliferation remained the main component of the mass and was composed of myofibroblastic-like spindle cells characterized by globular, irregular nuclei containing open chromatin and a prominent nucleolus. On the basis of the histopathologic lesions, the subcutaneous mass was diagnosed as IPT. Also, we detected a primo vessel-like structures in some areas of the IPT tissues. These were observed as novel thread-like structures and bundle of tubular structures. To our knowledge, this report is the first case of primo vessel-like structure in a dog with IPT.