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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 35, Issue 4 - Dec 2012
Volume 35, Issue 3 - Sep 2012
Volume 35, Issue 2 - Jun 2012
Volume 35, Issue 1 - Mar 2012
Selecting the target year
Characterization of G9 genotype porcine rotavirus isolated in Korea
Wang, Jun-Hui ; Lee, Seung-Chul ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 83~90
DOI : 10.7853/kjvs.2012.35.2.083
Porcine rotaviruses are the most common causes of viral gastroenteritis in piglets around the world. The major G genotypes of porcine rotaviruses causing diarrhea were G4, G5 and G11 genotypes. Recently, G9 genotype rotaviruses were problemed at swine farms and frequently recognized from diarrheic piglets. In this study, a porcine rotavirus (PoRV-1) was isolated from piglet showing diarrhea using MA104 cells and confirmed as rotavirus by electron microscopy, genomic RNA electropherotyping and indirect immunofluorescence antibody tests. The nucleotide sequence of the VP7 gene of PoRV-1 was determined and compared with those of other genotype rotavirus strains from other parts of the world. Also, the nucleotide sequences of VP4, VP6 and NSP4 genes of PoRV-1 were determined and compared with those of other rotavirus strains from other countries. The results showed that the PoRV-1 isolate belonged to the G9 genotype and the P, I and E genotypes of PoRV-1 were P, I5 and E1, respectively. The Korean G9 PoRV-1 isolate and its nucleotide sequence data would be usefully used for the development of porcine rotavirus vaccines in near future.
Seroprevalence of infectious bursal disease (IBD) and genetic sequence analysis of IBD virus
Kang, Mi-Seon ; Chu, Keum-Suk ; Cho, Hyun-Ung ; Han, Jae-Cheol ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 91~97
DOI : 10.7853/kjvs.2012.35.2.091
The strategy for infectious bursal disease (IBD) control and its success rate under field conditions depends on hygiene management, IBD field pressure, level, and variation in maternally derived IBD antibodies. This study investigated the level of IBD-specific antibody by ELISA and the prevalence of IBD virus by PCR in broilers, white-semi broilers, and Korean native chickens raised in Jeongeup, Jeonbuk. IBD-specific maternally derived antibodies were measured from 698 chickens and the mean titers of maternal antibodies were
in white-semi broilers, and
in Korean native chickens. At 2 weeks after vaccination, the geometric mean antibody titers of broiler, white-semi broiler, and Korean native chicken were
, respectively. According to sequence analysis of the variable virion protein 2 gene, 4 isolates were found to be very virulent IBDV, 9 isolates classical virulent, and 2 isolates intermediate plus vaccine strain.
High prevalence of Enterococcus spp. from dogs with otitis externa
Jo, Hyun-Jung ; Chae, Hee-Sun ; Kim, Hyun-Ju ; Kim, Min-Ju ; Park, Gyu-Nam ; Kim, Sang-Hun ; Chang, Kyung-Soo ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 99~104
DOI : 10.7853/kjvs.2012.35.2.099
Otitis externa (OE) is a frequent disease in the ear canals of dogs. To identify the pathogens causing OE in dogs and to determine their antimicrobial resistances, specimens were collected from animal hospitals in Daejeon. The isolates were examined by morphological and biochemical tests, 16S rRNA analysis and antimicrobial susceptibility tests. We analyzed correlation between the isolated pathogens and external factors of dogs such as breed, age, gender, ear mite, hair in ears and experience with antibiotic therapy. Thirty three strains of bacteria were isolated from 26 of the 68 heads of dogs with OE. The most isolated bacteria were Enterococcus faecalis (E. faecalis) followed by Staphylococcus aureus (Sta. aureus), Sta. pseudointermedius, E. faecium, E. avium and Streptococcus canis (Strep. canis) in order of frequency of occurrence. Isolation frequency of Enterococcus spp. and Staphylococcus spp. were 51.5% and 45.5%, respectively. E. faecalis and E. faecium isolates showed VanB phenotype, which is resistant to vancomycin but sensitive to teicoplanin were 58% and 25%, respectively. Nine isolates among total twelve isolates of E. faecalis were isolated from the dogs treated with antibiotics. There was no methicillin-resistant Sta. aureus (MRSA), but were MR-Sta. pseudointermedius (MRSP) (57.1%) and vancomycin-resistant (VR)-Sta. pseudointermedius (14.3%) (VRSP) showing VanB phenotype. However, vanA, vanB and vanC genes were not detected in VR isolates from the dogs. Taken together, VR-Enterococcus spp. (VRE) is one of the major pathogens in domestic animals, as well as community-and hospital-acquired infection.
Anti-apoptosis effects by Eimeria tenella infection in Madin-Darby bovine kidney cells
Lee, Hyun-A ; Hong, Sun-Hwa ; Chung, Yung-Ho ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 105~109
DOI : 10.7853/kjvs.2012.35.2.105
Apoptosis is a host defense mechanism that the cell uses to limit production of infectious pathogens. Although many bacteria, viruses and parasites can induce apoptosis in infected cells, some pathogens usually exhibit the ability to suppress the induction of apoptosis in the infected cells. Sophisticated evasion strategies of obligate intracellular parasites, in particular prevention of host cell apoptosis, are necessary to ensure successful replication. To study the ability of Eimeria tenella in this regard, in vitro experiments were performed applying Madin-Darby bovine kidney (MDBK) cells as host cell. We have demonstrated that productive infection of adherent cell lines by E. tenella resulted in an anti-apototic effect. This phenomenon was confirmed using in situ terminal deoxynucleotidyl transferase-mediated (TdT) deoxyuridine triphosphates (dUTP)-fluorescein nick end labeling (TUNEL) assay to detect apoptosis. Therefore, E. tenella could complete its cycle of productive infection while inducing anti-apoptosis in the infected cells. This finding might have implications for the pathobiology of E. tenella and other Eimeria species.
Prevalence of honeybee diseases in Incheon area in 2011
Ra, Do-Kyung ; Jeong, Cheol ; Lee, Joo-Ho ; Lee, Yun-Mi ; Kim, Kyoung-Ho ; Han, Tae-Ho ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 111~117
DOI : 10.7853/kjvs.2012.35.2.111
This study investigated the occurrence of honeybee diseases in Incheon area, at the point of great widespread of sacbrood disease in the country. Sixteen resident beekeeping apiaries; 3 native honeybee and 13 European honeybee apiaries were selected for this research. Over 20 adult bees were evenly collected from the most colonies of each apiary three times (March, June, November) within a year. In this work, 13 honeybee diseases including 7 viral diseases, 2 bacterial diseases, 2 fungal diseases, and 2 parasitic diseases were detected by preliminary inspections and PCR. As a result, viral infections were confirmed at 34 among 48 apiaries (70.8%) over the entire examination period. Parasitic diseases showed the highest detection rate of 45.8%, which are detected in 44 among 96 cases. In the seasonal prevalence, 30 cases (15.6%) of 7 pathogens were detected from 14 apiaries in March, 50 cases (24.0%) of 9 pathogens and 56 cases (26.9%) of 9 pathogens were detected from all apiaries in June and November, respectively. Nosema was shown to be the most prevalent pathogen from March to November, followed by sacbrood virus (SBV) and stonebrood. The spread of SBV infection in Incheon would be under-estimated by the increasing of detection rate over the time. Especially, Chinese sacbrood virus was detected from 4 European honybee apiaries, but clinical symptoms were not found. No chalkbrood, acute bee paralysis virus, and chronic bee paralysis virus were detected in this study. The effective therapy and preventive measures should be prepared for beekeeping industry.
Investigation for freshness and nutritive components of the eggs sold in Incheon
Hong, Seong-Hee ; Ra, Do-Kyung ; Yun, Ga-Ri ; Joung, Yun-Joung ; Nam, Ji-Hyeon ; Cheong, Eun-Ha ; Lee, Jung-Goo ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 119~128
DOI : 10.7853/kjvs.2012.35.2.119
This study was performed to investigate the freshness and nutritive components of eggs from Incheon area. The eggs of 64 brands collected from 7 hypermarkets and 6 brands collected from farms were examined to test the Haugh unit (HU), egg yolk index and specific gravity. HU and specific gravity were higher in farm eggs than in hypermarket eggs (P<0.05), and there was no difference in the egg yolk index. Among hypermarket eggs, HU and egg yolk index were higher in the graded eggs than in non-graded eggs (P<0.05), and specific gravity was not different between them. HU and egg yolk index of the eggs collected in March were higher than those in August (P<0.05). To monitor the bacterial contamination, total coliforms, total bacterial counts (TBC) and Salmonella enteritidis were examined. Salmonella enteritidis and total coliforms were not detected from all eggs. TBC of farm eggs were less than
CFU/mL in all samples and TBC of hypermarket eggs were less than
CFU/mL in 187 samples,
CFU/mL in 3 samples and
CFU/mL in 2 samples, respectively. Antimicrobial residues were not detected from all eggs tested. The vitamin E contents in the vitamin E enriched eggs from 6 brands hypermarket were 1.98~22.96 mg/yolk100 g (
mg/yolk100g), and those of 5 brands among them were higher than the average of ordinary eggs. In one brand egg, vitamin E content was lower than the average of ordinary eggs. The vitamin E contents of ordinary eggs were 1.75~16.36 mg/yolk100 g (average
mg/yolk100 g) and there were no price differences between vitamin E enriched eggs and ordinary eggs. In the contents of vitamin E, docosahexaenoic acid (DHA) and cholesterol, there were no significant differences according to the price. These results suggests that egg production, distribution, and management system should be improved for supplying fresh eggs to consumers at reasonable prices.
Serological and virological investigation of pestiviruses in Korean black goats
Oem, Jae-Ku ; Lee, Eun-Yong ; Byun, Jae-Won ; Kim, Ha-Young ; Kwak, Dong-Mi ; Song, Hee-Jong ; Jung, Byeong-Yeal ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 129~131
DOI : 10.7853/kjvs.2012.35.2.129
Blood samples were collected from 672 goats in 60 farms from five provinces of Korea between November 2009 and August 2011. The prevalence of antibodies to pestiviruses was investigated. The examination for antibodies was performed using an enzyme-linked immunosorbent assay (ELISA) detecting antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV). All blood samples were screened using reverse transcription-polymerase chain reaction (RT-PCR) with primer pairs specific to common pestivirus genome regions. The observed individual seroprevalence was 1.49% and herd seroprevalence was 11.67%. Also, the specific genomes to pestiviruses were detected in 3 out of the 915 clinical samples (0.45%). Based on the nucleotide sequence data, detected pestiviruses were belonged to two BVDV type-1 and one BVDV type-2. The pestivirus infection has been occurred among Korean black goats. However, our results indicate that the prevalence of pestiviruses in black goats was not significantly higher on farms with cattle.
Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis
Rahman, Md. Siddiqur ; Chakrabartty, Amitavo ; Islam, Md. Taohidul ; Sarker, Roma Rani ; Alam, M.E. ; Uddin, Muhammad Jasim ; Akther, Laila ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 133~137
DOI : 10.7853/kjvs.2012.35.2.133
Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.
Prevalence of major legal communicable diseases in bovine and swine in Jeonbuk province (2004~2008)
Hur, Boo-Hong ; Lee, Jeong-Won ; Song, Hee-Jong ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 139~145
DOI : 10.7853/kjvs.2012.35.2.139
Prevalence of major legal communicable diseases in bovine and swine had been monitored in Jeonbuk province from year 2004 to 2008. At least 1 communicable disease had been reported in 687 heads from 68 bovine farms and 17 farms (25.0%) of the 68 positive farms had 1~2 additional outbreaks during the surveillance. By disease, enzootic bovine leukosis, Johne's disease and Akabane disease were occurred in 53 farms (582 heads), 14 farms (100 heads) and 1 farm (5 heads), respectively. Swine communicable diseases were occurred in 4,466 heads from 63 swine farms and 18 farms (28.6%) of the 63 positive farms had 1~2 additional outbreaks during the surveillance. By disease, Aujeszky's disease (AD), porcine epidemic diarrhea (PED), classical swine fever (CSF), porcine reproductive and respiratory syndrome (PRRS), porcine transmissible gastroenteritis (TGE), atrophic rhinitis (AR) and Japanese encephalitis in swine (JE) were occurred in 20 farms (70 heads), 20 farms (2,817 heads), 12 farms (258 heads), 6 farms (1,257 heads), 1 farm (50 heads), 1 farm (2 heads) and 1 farm (10 heads), respectively. In total, 10 communicable diseases (1 species of first-class, 3 species of second-class, and 6 species of third-class) were reported. The first-class diseases were CSF. Johne's disease, and Aujeszky's disease. JE was the second-class and Akabane disease, enzootic bovine leukosis (EBL), PED, PRRS, TGE and AR were third-class diseases.
A case of canine chondrosarcoma from the rib
Kim, Sung-Jae ; Kwak, Ho-Hyun ; Park, In-Chul ; Yoon, Byung-Il ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 147~151
DOI : 10.7853/kjvs.2012.35.2.147
We report large tumors of the left thoracic cavity, which arose from the ribs, were diagnosed as chondrosarcoma in a 4-year-old male mixed dog. The dog was presented with swelling in the left side of the chest wall and lameness. The masses were found to be circumscribed, whitish-grey, large and firm. The cut surface revealed whitish-grey lobules of varying size with cartilaginous consistency. Those were subsequently metastasized to the lung and mandibular lymph node. Histologically, the tumors were made up of clusters of chondroid cells having pleomorphic hyperchromatic nuclei with occasional mitosis. A special and immunohistochemical staining was performed to confirm the diagnosis. Chondroid matrix in tumor showed a positive reaction for alcian blue-periodic acid-Schiff staining. Immunoreactivity to S-100 proteins was present in the cytoplasm and/or nucleus of chondrocytes in tumor. The final diagnosis was grade III chondrosarcoma in the rib, considering histological features in grading criteria.
Primary splenic hemangiopericytoma in a dachshund dog
Kim, Sung-Jea ; Yang, Don-Sik ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 153~157
DOI : 10.7853/kjvs.2012.35.2.153
A 7-year-old, castrated male dachshund dog with tumor of the spleen portion was referred to the Kangwon National University. The tumor removed surgically and tumor size was 6~7 cm. Histopathologically, this neoplasm was the presence of perivascular whorls of fusiform cells. The cells also arranged in interlacing bundles or storiform patterns. And this neoplasm consisted of spindle cells that often formed distinct whorls around a central capillary. Immunohistochemical analysis revealed multi-focally immunoreactivity for
-smooth muscle actin, whereas not immunoreactive for desmin, S-100, von Willebrand factor. On the base of the histological and immunohistochemical results, this neoplasm was diagnosed as a canine hemangiopericytoma.
A case of hypothyroidism concurrent with myxedema coma in a Cocker spaniel dog
Kim, Yun-Hye ; Kim, Jun-Hwan ; Lee, Hyun-Seok ; Cho, Ho-Seong ; Park, Jin-Ho ; Park, Chul ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 159~163
DOI : 10.7853/kjvs.2012.35.2.159
A 4-year-old female Cocker spaniel weighing 7.74 kg was presented with a seven-month history of lethargy and skin problems. On the basis of clinical history, physical examination, laboratory tests, electrocardiogram, radiography, ultrasonography, and thyroid function tests, the dog was diagnosed as hypothyroidism. The dog was administered levothyroxine and presented a good response to the therapy. However, uncontrolled for about 6 months, the dog was re-admitted to hospital with depression and three seizure episodes. The dog was diagnosed as hypothyroidism with concurrent myxedema coma and gallbladder mucocele. With fluid therapy and administration of levothyroxine, the dog was successfully controlled for 3 months after starting treatment. This report describes the clinical features and successful treatment of a Cocker spaniel dog with concurrent hypothyroidism and myxedema coma.
A case of aspergillosis in commercial domestic ducks
Chu, Keum-Suk ; Kang, Mi-Seon ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 35, issue 2, 2012, Pages 165~168
DOI : 10.7853/kjvs.2012.35.2.165
A case of aspergillosis was observed in a flock of 5~10 days old commercial domestic ducks in Jeongeup. The clinical signs were depression and breathing by gasping or rapid opening of the bill. Grossly, lung was attached to pleura with white cheesy plaques. Numerous nodules was found in lung. Histopathologically, severe granulomatous pneumonia with intralesional fungal hypha were observed in the lung. Aspergillus spp. colonies was isolated from fungal culture and identified by polymer chain reaction.