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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 35, Issue 4 - Dec 2012
Volume 35, Issue 3 - Sep 2012
Volume 35, Issue 2 - Jun 2012
Volume 35, Issue 1 - Mar 2012
Selecting the target year
Evaluation of Japanese encephalitis virus vaccine strains currently used in pigs by molecular characterization
Lee, Jeong-Ah ; Yang, Dong-Kun ; Kim, Ha-Hyun ; Kim, Sun-Young ; Nah, Jin-Ju ; Cho, Soo-Dong ; Song, Jae-Young ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 169~174
DOI : 10.7853/kjvs.2012.35.3.169
Japanese encephalitis virus (JEV) is one of the main causes of viral encephalitis in human and animals. For over 30 years, a live attenuated JEV vaccine strain has been used in the veterinary field, and it is required to conduct quality evaluation studies on the commercial vaccines. For the quality control of live attenuated JEV vaccine, we investigated the nucleotide sequence similarity of prME gene derived from five JEV vaccines commercially available in pigs in Korea. The Vero cells infected with JEV vaccines showed specific cytopathic effect, which was characterized by rounding and detached cells. In the phylogenetic analysis, all of the vaccine strains showed a close relationship with the original vaccine seed strain (Anyang 300) and clustered into the genotype 3. In comparison of the nucleotide and deduced amino acid sequences of prME genes with the original strain, all JEV vaccine strains showed high amino acid similarity ranging from 98.9% to 99.5%, but had several point mutations, probably due to high mutation rates of viral RNA polymerase by several virus passages. Even though the current JEV vaccine strains have been maintained and produced for a long period of time, the genetic characterization of them have been rarely changed. However, since the mid 1990`s, molecular epidemiology of JEV has been changed sharply from genotype 3 to genotype 1 in Korea, further studies on new vaccine strains to genotype 1 is required for more effective prevention in the field.
Monoclonal antibodies against porcine group C rotavirus VP6
Yoon, Young-Sim ; Lee, Seung-Chul ; Woo, Sang-Kyu ; Cho, Kyoung-Oh ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 175~182
DOI : 10.7853/kjvs.2012.35.3.175
Rotaviruses have been known to be a major etiological agent of gastroenteritis in both infants and young animals. Subsequently new rotaviruses, which were morphologically indistinguishable but antigenically and electrophoretically distinct with each other, were reported from several animals throughout world including Korea. These new rotaviruses were named as non-group A or group B or group C rotaviruses and so on. It has been very difficult to isolate and grow the non-group A rotaviruses in vitro, and this has greatly limited the characterizations of non-group A rotaviruses and serological studies. In this study, monoclonal antibodies (MAbs) against porcine non-group A rotavirus were produced and characterized. The VP6 gene of porcine group C rotavirus Korean isolate(#06-52-1) was cloned and expressed. For expression of VP6 gene, baculovirus expression system was applied. The VP6 gene and expressed protein in the recombinant virus were confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test and Western blot, respectively. The expressed VP6 was used for MAbs production. The MAbs produced in this study would be promising as diagnostic reagents for detection of group C rotavirus infection.
Environmental survey on the vectors and hosts of Tsutsugamushi disease in Jeonnam province, Korea
Song, Hyeon-Je ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 183~189
DOI : 10.7853/kjvs.2012.35.3.183
Tsutsugamushi disease or scrub typhus cause by Orientia tsutsugamushi is an endemic disease in Korea. Chigger mites and field rodents play roles in transmission of the disease by the vector and host of the agent. The purpose of this study is to investigate the density of the chigger mites and field rodents due to environmental factors such as temperature, relative humidity, soil thickness and the various vegetations to the 9 field rodent collection sites. The total 62 field rodents was captured by the Sherman collapsible traps from April to October 2009 at the Jangseong of Jeonnam Province, Korea. The trapping rate of the field rodents by the different collecting sites was dominant at subside storage water (24%), bush near by dam (22%), bank around field (20%), followed by 18% of grassy field and surround cattle shed. The distribution of chigger mites by the different collecting sites was the highest at Bush near by dam (28.7%). And the sites of subside storage water, bank around field and surround cattle shed were 20.4%, 18.8%, 16.4%, respectively. On the other hand the collecting sites of stream bank and ridges between rice paddies were not collected. The temperature to the collecting sites was showed
in June and
in October which was higher than April (
), whereas lower than May (
) and September (
). The highest number of mites was collected at
and 46.6% relative humidity in October. The chigger mites and field rodents were highly collected between 18 and 24% at the sites where are loosely in the superficial layers of the soil from 8.0 cm to 10.2 cm. Total 25 species of vegetation were distributed at the collecting sites. In the present study, strong evidence was found that bank around field and grassy field were provided for the prevalence sites of tsutsugamushi disease.
Effect of Ginkgo biloba extract on the survival rate in lipopolysaccharide-induced sepsis model mice
Rhee, Ki-Jong ; Kim, Hyeon-Cheol ; Jung, Bae-Dong ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 191~195
DOI : 10.7853/kjvs.2012.35.3.191
Accumulated evidence indicate that Ginkgo biloba extract (EGb 761) acts as an antioxidant and scavenger of free radicals as well as influencing apoptotis. Earlier studies have employed the inflammatory agent lipopolysaccharide (LPS) to induce severe sepsis. In the present study, we examined whether the intraperitoneal injection of EGb 761 increases the survival rate of mice in the LPS-induced severe sepsis model. The survival rate was significantly increased by 30% in mice administered with 100 mg/kg of EGb 761 but not in mice administered with 50 mg/kg EGb 761. In addition, pre-treatment with EGb 761 increased the survival rate (30%) but post-treatment with EGb 761 did not. These results suggest that EGb 761 may have clinical potential in preventing sepsis induced mortality.
Physiochemical analysis, toxicity test and anti-bacterial effect of practically detoxified sulfur
In, Dong-Chul ; Yu, Do-Hyeon ; Park, Chul ; Park, Jin-Ho ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 197~205
DOI : 10.7853/kjvs.2012.35.3.197
Despite of a long history of the sulfur on the disease healing effect, there were limited ways of applying sulfur to animal and human. We have developed the detoxified sulfur (non toxic sulfur) method to make it practical and mass production possible through laboring for many years. This study practiced scanning electron microscope (SEM), Energy dispersive X-ray spectrometer (EDS) and secondary ion mass spectrometry (SIMS) analysis to investigate the physicochemical aspect of detoxified sulfur. We also performed the oral toxicity experiment to mice, and anti-bacterial test of the detoxified sulfur. Based on the SEM, EDS and SIMS results, the united particles in the mass form with the similar component intensity with the raw sulfur were observed, and hydrogen sulfide ion (HS-) component which is regarded as a toxic matter, was decreased after detoxification. Indeed, toxicity test on the mice (10 males, 10 females) showed no clinical, histopathological changes with the 5 times amount (2,500 mg/kg) of the actual doses. However, the male-mice showed decreased in body weight by 23.6%, 24.3% in the 7th, 14th day, respectively, after detoxified sulfur. Moreover, the female-mice administered the detoxified sulfur showed decreased in body weight by 28.7% (P<0.05) than that in the control group on the 14th day. The result of antibacterial test on the detoxified sulfur showed antibacterial effect (27%) to inhibit the growth of Staphylococcus aureus. It is shown that detoxified sulfur can be used as feed additive and has an affect on the farm perfomance.
Investigation of trunk conformation and reproductive activity in Donggyeongi
Choi, Seog-Gyu ; Sung, Gi-Chang ; Lee, Eun-Woo ; Park, Chang-Eun ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 207~213
DOI : 10.7853/kjvs.2012.35.3.207
The trunk conformations of the Gyeongju Donggyeongi (GD), Korean native dog was measured in the present study. Also, the information of reproductive activity was investigated in GD dogs. A total of 73 dogs were randomly selected out of 121 dogs in the Gyeongju County in July 2010, which were registered by inserting electronic chips in the withers. The hair colors of the GD were white, yellow, black and tiger brindle, the mean withers heights were
for female and male dogs, respectively The mean body length to withers height ratios were 117.63% and 116.4% for the female and male dogs. Concerning the reproductive capability of the GD, the mean litter number was
, the optimal mating time was
days after the rutting season and the mean gestation period was
days. The results of this study would be useful for compiling the official standard for the adult GD.
Development of TaqMan
probe-based real-time PCR for rapid identification of beef, pork and poultry meat
Koh, Ba-Ra-Da ; Kim, Ji-Yeon ; Na, Ho-Myung ; Park, Seong-Do ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 215~222
DOI : 10.7853/kjvs.2012.35.3.215
probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only >50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated (
/5 min) meat samples. In conclusion, it can be suggested that the
probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.
A survey for Pb, Cd and microbiological contamination from by-products of cattle in Incheon city
Nam, Ji-Hyeon ; Joung, Yun-Joung ; Yun, Ga-Ri ; Hong, Seong-Hee ; Ahn, Eun-Jung ; Lee, Jung-Goo ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 223~230
DOI : 10.7853/kjvs.2012.35.3.223
This study was carried out to investigate the heavy metal and microbiological hazards on by-products (liver, omasum, small intestines) of cattle. From April to October in 2011, one hundred and twenty samples were equally collected from slaughterhouse and meat by-product markets in Incheon city. The total bacteria counts and E. coli count were applied to assess the microbiological quality. Food borne bacteria including Salmonella spp., Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens and E. coli O157:H7 were also determined. The results were obtained as follows: The undesirable grade (more than
) was detected in the by-product from 18.3% (slaughterhouse) and 23.3% (by-product markets). The frequency of generic E. coli (more than
) from the slaughterhouse was 20.0%, whereas that of the meat by-product markets was 26.7%. Of the samples from slaughterhouse, 3 (5.0%), 5 (8.3%), and 12 (20.0%) samples were contaminated with Salmonella spp, S. aureus, and C. perfringens, respectively. S. aureus and C. perfringens were also detected in 6 (10.0%) and 25 (41.7%) samples in the meat by-product markets, respectively. Nine of 11 S. aureus isolates harbored toxin gene. However, the cpe gene of C. perfringens was not detected among the 37 isolates. The detection rate was higher in August than in February, April and June. The levels of Cd and Pb in all the samples tended to be low (<0.2 mg/kg). This preliminary data could be used for legislation on the regulation and control of microorganism and heavy metal in by-products of cattle.
Survey of artificial sweetener contents as food additives in children`s preferred dairy products
Kang, Kyoung-Sook ; Jeon, Hye-Chang ; Park, Hyoung-Sook ; Choi, Tae-Seok ; Shin, Bang-Woo ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 231~237
DOI : 10.7853/kjvs.2012.35.3.231
In this study, monitoring of food additives as an artificial sweeteners on favorite dairy products of children, which are deal at supermarket, was performed. We analyzed aspartame, acesulfame-K, sodium saccharin, and sucralose from total 206 dairy products which are fermented milk, ice-creams and others. Aspartame was detected from 3 cases of fermented milk. Sucralose was detected from 10 cases of processed milk and 2 cases of fermented milk. The detected concentration was followed [average (minimum~maximum)]: aspartame 34.6 mg/kg (2.9~97.9 mg/kg), sucralose 29.8 mg/kg (5.5~67.7 mg/kg). The level of intakes of detected sweeteners were evaluated by comparisons with the acceptable daily intake (ADI) data established by the joint FAO/WHO expert committee on food additives. The estimated daily intake (EDI) of aspartame and sucralose per day were 0.37 mg/day, 0.20~0.32 mg/day. The intake levels were 0.05~0.8%, which were all within safe levels.
A case of Aeromonas veronii infection in Israeli carp (Cyprinus carpio): phylogenetic analysis and antimicrobial resistance
Yi, Seung-Won ; You, Myung-Jo ; Lee, Hae-Beom ; Shin, Gee-Wook ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 239~243
DOI : 10.7853/kjvs.2012.35.3.239
We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe ulcerative necrosis in the skin. The necropsy showed yellowish ascites, necrosis in liver and enlargement of kidney and spleen in the morbid fish. In blood agar for culturing bacteria, three different colonies were identified as A. veronii, Plesimonas shigelloides and Shewanella putrefaciens by phylogenetic identification using 16S rRNA or gyrB gene sequences. A. veronii was the most dominant species among them and was resistant to ampicillin, nalidixic acid and oxytetracycline.
Use of thermography in lameness caused by musculoskeletal disease in Thoroughbred mare
Park, Hyoung-Joon ; Song, Yun-O ; Lee, Jin-Ju ; Ji, Chang-Moo ; Jung, Hae-Won ; Kim, Suk ; Cho, Kyu-Won ; Cho, Jae-Hyeon ; Kim, Jong-Shu ; Moon, Oun-Kyong ; Song, Young-Min ; Kim, Chung-Hui ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 245~250
DOI : 10.7853/kjvs.2012.35.3.245
The mare had lameness and asymmetry edematous on its tarsal and metatarsal joints at the initial physical examination. The pain was elicited with a palpation along the metatarsal articulations. No significant abnormalities were detected in the screening test. However, thermographic images revealed a significant increase in the surface temperature at the joint of the hindlimb when compared to the reference range. At necropsy, an irregularity of the surface and excessive synovial fluid were observed on the right tarsal joint. No bacterial growth was shown in the cultures of synovial fluid. Staphylococcus aureus was detected in the subcutaneous discharge. Taken together, the thermography images were very useful in localizing the area of injury and were an effective diagnostic methodology for assessing lameness.
Radiological assessment of pectus excavatum in a Pekingese dog
Cho, Sung-Jin ; Hong, Sun-Hwa ; Chung, Yung-Ho ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 35, issue 3, 2012, Pages 251~254
DOI : 10.7853/kjvs.2012.35.3.251
Pectus excavatum (PE) is a ventral chest wall deformity, also known as funnel chest, sunken chest, chondrosternal depression or koilosternia. The 4 months old, 1.3 kg intact-female Pekingese dog was evaluated for acute semicoma and convulsion. The client reported that this patient have had chronic loss of appetite, intermittent dyspnea and palpable sunken breast. The other littermates did not show any abnormalities. On physical examination, cachexia (BCS 1/5), concave sternum, flatten thoracic cavity and cardiac murmur were observed. On radiographic study, the caudal sternum cave to vertebrae and narrowing thoracic cavity. The severities of thoracic deformity were evaluated by deformation indices such as-Frontosagittal index (FSI) and vertebral index (VI). Moderate to severe PE was founded by the radiological measurements.