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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 35, Issue 4 - Dec 2012
Volume 35, Issue 3 - Sep 2012
Volume 35, Issue 2 - Jun 2012
Volume 35, Issue 1 - Mar 2012
Selecting the target year
Detection of foot-and-mouth disease virus and coxsakievirus in the soil and leachate of modeled carcass burial site
Cho, Ho-Seong ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 255~261
DOI : 10.7853/kjvs.2012.35.4.255
Foot and mouth disease (FMD) is highly infectious disease of cloven-hoofed animals, particularly cattle, sheep, pigs and goats. Last outbreak reported in November, 2010 induced the enormous social and economical impacts. Culling of infected animals, movement control, and vaccination are the major control measures of FMD. The aim of this study was to detection foot-and-mouth disease virus (FMDV) in the soil and leachate from modeling burial for pig carcass as measured by real-time reverse transcriptase polymerase chain reaction (RT-PCR). FMDV and Coxsakievirus B1 (CVB1) were detected in soil by week 16 and Coxsakievirus B1 (CVB1) by weeks 12, respectively. FMDV and CVB1 also detected by weeks 8 in the leachate. Results from this study provides an evidence that FMDV could be inactivated for safe of pig carcasses infected with FMDV within 4 month in the carcass burial site.
Investigation of occurrence factors on brucellosis-outbreak farm in Korea
Kim, Ji-Yeon ; Kang, Shin-Seok ; Her, Moon ; Lee, Kichan ; Sung, So-Ra ; Gu, Jung-Hui ; Kang, Sung-Il ; Lee, Hyang-Keun ; Kim, Yu-Jin ; Kim, Dong-Gil ; Jung, Suk-Chan ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 263~268
DOI : 10.7853/kjvs.2012.35.4.263
From the end of July 2012, several cases of abortion have been happened at the Korean indigenous cattle farm with 124 heads in Chungbuk province, Korea. Serological tests such as Rose-bengal test (RBT) and standard tube agglutination test (STAT) have been performed according to the standard official protocols of bovine brucellosis and 41 cattle turned out to be brucellosis-positive simultaneously. To find out the main factors of brucellosis outbreaks and spreads, additional serological, etiological and molecular investigation were applied. Totally, 11 B. abortus were isolated from 10 cattle's specimens including lymph-nodes and/or testis, and drinking water in cowhouse. In genotyping by multi-locus VNTR assay (MLVA) using 17 loci markers, the present B. abortus isolates were shown all the same pattern, D1 genotype, which has been reported in Gyeonggi and Gangwon province, Korea. These results suggest that the input of brucellosis might come from neighboring farms directly or indirectly, even if by unknown factor and expansion within farm would accelerate by materials related with aborting cows.
Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis
Sung, So-Ra ; Kim, Ji-Yeon ; Her, Moon ; Lee, Kichan ; Gu, Jeong-Hui ; Kang, Sung-Il ; Lee, Hyang-Keun ; Kim, Suk-Mi ; Jung, Suk-Chan ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 269~273
DOI : 10.7853/kjvs.2012.35.4.269
A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.
MLVA typing of Brucella canis isolated from dogs in Gyeongbuk province, Korea
Kim, Seong-Guk ; Sohn, Joon-Hyung ; Jang, Young-Sul ; Kim, Sang-Yoon ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 275~281
DOI : 10.7853/kjvs.2012.35.4.275
Canine brucellosis is the zoonosis in worldwide and Brucella (B.) canis is a facultative intracellular pathogen that has a very limited host. MLVA-16 (Multilocus VNTR analysis) is a efficient method for genotyping of Brucella species. Various methods have been established for genotyping of Brucella species, but most of analytical method is lack reproducibility and limited capability to differentiate them. B. canis isolates (n=73) from 7 farms in Gyeongbuk province in 2003~2010 were analyzed using 16 VNTR loci. Automatic electrophoresis system was utilized for more high throughput and rapid simple discrimination. Thirty two genotypes were identified from 73 B. canis isolates. MLVA could contribute to molecular typing for epidemiological evaluation of canine brucellosis.
PFGE patterns of Streptococcus suis isolates from diseased pigs in Gyeongbuk province, Korea
Kim, Seong-Guk ; Kim, Young-Hoan ; Lee, Hong-Young ; Yun, Mun-Jo ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 283~288
DOI : 10.7853/kjvs.2012.35.4.283
Streptococcus(S.) suis is a pathogen, causing meningitis, septicemia and sudden death in weaning piglets as well as fattening pigs. Using multiplex PCR method based S. suis capsular genes, 61 S. suis isolates was classified as serotypes 2, 7, 9 and untypable. Genotyping of S. suis isolates was analysed by PFGE pattern with treated Sma I restricted enzyme. Of the 61 S. suis, 25 (40.9%) were serotype 2, 6 (9.8%) were serotype 7, 5 (8.2%) were serotype 9, and 25 (40.9%) were untypable, respectively. Twenty four PFGE patterns were detected in this study and also PFGE patterns were classified according to serotype; serotype 2 was classified as 6 genotypes, serotype 7 was 5 genotypes, serotype 9 was 3 genotypes, and untypable was 11 genotypes, respectively.
A newly developed consensus polymerase chain reaction to detect Mycoplasma species using 16S ribosomal RNA gene
Hong, Sunhwa ; Park, Sang-Ho ; Chung, Yung-Ho ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 289~294
DOI : 10.7853/kjvs.2012.35.4.289
Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifically to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma genus-specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.
Isolation and antimicrobial susceptibility of microorganisms from milk samples of dairy goat
Kim, Hye-Ra ; Jung, Ji-Young ; Kim, Seon-Deuk ; Park, Jun-Young ; Cho, In-Young ; Shin, Sung-Shik ; Son, Chang-Ho ; Ok, Ki-Seok ; Hur, Tai-Young ; Jung, Young-Hun ; Choi, Chang-Yong ; Suh, Guk-Hyun ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 295~305
DOI : 10.7853/kjvs.2012.35.4.295
The aim of this study was to isolate microorganisms from half milk samples of dairy goats by California mastitis test (CMT) during the lactation period and to further investigate the susceptibility of isolated organisms to antimicrobial drugs. From a total of 235 half milk samples with CMT scores of 2 or above from 366 dairy goats distributed throughout Jeonnam province, microorganisms were isolated from 198 (83.5%) samples either singly (99.0%) or in combination (1.0%). The most prevalent microorganism was the coagulase-negative Staphylococcus spp., (44.4%, n=88) followed by Staphylococcus aureus (24.2%, n=48), Escherichia coli (11.1%, n=22) and Streptococcus spp. (7.6%, n=15). Isolated bacteria also included Bacillus spp. (2.5%, n=5), Pseudomonas spp. (2.5%, n=5), Micrococcus spp. (1.5%, n=3), Corynebacterium spp. (1.5%, n=3), Enterococcus facium (1.0%, n=2), Morganella morganii (0.5%, n=1) and Streptococcus agalactiae (0.5%, n=1). During the summer season, a high prevalence of all microorganisms were observed in which Staphylococcus spp. (30.8%), Escherichia coli (8.6%), and Streptococcus spp. (5.6%) were among the most prevalent bacteria isolated. Staphylococcus spp. was also shown to be high in the winter (21.7%). In most samples, the presence of bacterial pathogens in goat milk led to the increase in the total somatic cell count (SCC). Most of the half milk samples of dairy goats with bacterial contamination showed SCC of
(90.4%). Minor pathogens (11.4%) were more detected from milk samples with SCC of <
than major pathogens (4.1%), while the major pathogens tended to be higher from samples with SCC of
. Susceptibility of these bacteria to 12 antimicrobial agents was tested by the Kirby-Bauer disc diffusion method. Results indicated that more than 90% of bacteria isolated from CMT 2+ dairy goat half milk samples were susceptible to trimethoprim/sulfamethoxazole, amoxicillin/clavulanic, enrofloxacin and cephalothin while they were resistant to tetracycline (44.7%).
Effect of recombinant yeast producing Sus scrofa ferritin Heavy-chain on piglets
Choi, Young-Jun ; Lim, Hwan ; Kim, Hyeon-Cheol ; Kim, Jong-Taek ; Rhee, Ki-Jong ; Jung, Bae-Dong ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 307~312
DOI : 10.7853/kjvs.2012.35.4.307
Iron deficiency anemia is also recognized as a serious disorder in many livestock, especially, piglets. We previously studied that the iron-fortified yeast (Saccharomyces cerevisiae) producing Sus scrofa ferritin heavy-chain (FER) was bioavailable to mice with iron deficiency. In this study, we determined whether FER could improve iron deficiency in piglets. The bioavailability of FER was examined by measuring body weight gain, hemoglobin concentration and hematocrit value in suckling and weaning piglets. We found that FER significantly increased hemoglobin value and the hematocrit ratio in suckling piglets (P<0.05). Furthermore, FER treatment significantly enhanced body weight gain in both groups of the suckling and weaning piglets (P<0.05). These results suggest that the iron-fortified recombinant yeast strain is helpful in iron absorption in piglets.
Survey in consumers and distribution stages bacteriological analysis for fresh raw beef in Gwangju area, Korea
Na, Ho-Myung ; Bae, Seong-Yeol ; Koh, Ba-Ra-Da ; Jang, Mi-Sun ; Sung, Chang-Min ; Kim, Ji-Yeon ; Park, Heon-Gyu ; Mun, Yong-Un ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 313~319
DOI : 10.7853/kjvs.2012.35.4.313
Consumer's preference and microbial inspections on fresh raw beef were carried out to understand the actual market status in Gwanju, Korea. Over 15 questions on questionnaire by 1,111 randomly selected respondents between April and May in 2011, results showed 65.5% positive on eating fresh raw beef, 63.8% negative on good hygiene condition of fresh raw beef, and 72.5% positive on the secure of the hygiene-safety for priority program, respectively. For microbial inspections, a total of 302 samples were collected from fresh raw beef purchased from slaughterhouse (n=122), transport (n=69) and consumer (n=81) stage, from lettuce (n=30) at consumer stage. The aerobic plate count (APC), E. coli count and food borne bacteria such as Salmonella spp., Listeria monocytogenes, Staphylococcus(S.) aureus and E. coli O157:H7 were tested in the samples. As results, the level of count on APC of fresh raw beef ranged
from transport stage and
from consumer stage. The level of count on E. coli of fresh raw beef ranged
from transport stage and
from consumer stage. In total, 26 S. aureus were isolated, 10 (14.5%) from fresh raw beef at transport stage, 12 (14.8%) from fresh raw beef and 4 (13.3%) from lettuce at consumer stage. Enterotoxin of S. aureus was not detected among 26 isolates. All S. aureus isolates were typed using a DiversiLab
rep-PCR system for genetic similarity test, showing over 95% of genetic relationship amon isolates.
Suggestions for better HACCP system operation in butcher shops
Hong, Chong-Hae ; Kang, Cheon-Kun ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 321~325
DOI : 10.7853/kjvs.2012.35.4.321
Hazard Analysis Critical Control Point (HACCP) system application is expanded to food chain from farm to table to supply safe livestock products with consumers in Korea. The objectives of this study were to analyze the HACCP operating conditions in butcher shops and to suggest for better HACCP implementation. We analyzed the HACCP inspection reports for butcher shops implemented by the Korea Livestock Products HACCP Accreditation Service from 2007 to 2009 and from 2010 to 2012 to compare the rate of HACCP non-compliances. The overall rate of non-compliances was higher in 2010~2012 than 2007~2009. The current inadequate HACCP operating level was directly related with the business size. Ninety-nine percent of the butcher shops were very small business with under 10 employees in Korea, and these small-sized businesses usually had great difficulties in implementing HACCP due to lack of technical expertise and financial resources. To overcome the obstacles and to facilitate more effective HACCP application and operation in butcher shops, government should play a leading role in developing flexible and simplified HACCP-based system which merges both the pre-requisite and HACCP principles.
The effects of an animal-assisted therapy (AAT) program on depression and self-esteem of adolescents as victims of school violence
Park, Hyoung-Joon ; Kim, Chung-Hui ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 327~332
DOI : 10.7853/kjvs.2012.35.4.327
This study aimed to see if as companion animals living with human beings, dogs could provide emotional stability or play a therapeutic role for human beings. Of 12 middle school boys as victims of school violence, 6 were assigned to the experimental group that was provided with an animal-assisted therapy program over a total of 12 sections, one section per week, 60 minutes per section, between September and November 2011, followed by post-test 1 and, one month later, post-test 2. In the experimental composition, the remaining 6 boys were assigned to the comparison group that was allowed to read freely. Analysis was performed by using the scales of depression and self-esteem, and the results showed that the experimental group provided with the animal-assisted therapy program generated more significant therapeutic effects than the comparison group. The therapeutic effects were found to persist one month after the completion of the program. Therefore, it is believed that sensitive adolescents who suffer from depression or have lost self-esteem because of school violence can significantly benefit from an animal-assisted therapy program by reducing the recovery time or by getting lots of help with emotional stability. Further research on the basis of this study is expected to help adolescents with emotional therapy in other areas.
Investigation of bovine leukemia virus infection in dairy farms of northern Gyeonggi province, Korea
Jung, Kwang ; Shim, Hang-Sub ; Baek, Jin-Joo ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 333~337
DOI : 10.7853/kjvs.2012.35.4.333
This study was carried out to investigate the prevalence of bovine leukemia virus (BLV) infection and to compare the results of enzyme-linked immunosorbent assay (ELISA) and nested polymerase chain reaction (nPCR) in dairy farms in northern Gyeonggi province from August through December 2011. A total of 625 dairy cattle from 14 dairy farms were tested for antibodies against BLV using commercially available ELISA test kit. The overall seroprevalence of BLV infection was 76.3%. The seroprevalence of diary cattle according to age was the highest at 61~72 months (88.0%, P<0.001). Two hundred fifty one dairy cattle from 7 diary farms were tested ELISA and nPCR. The kappa value of BLV between ELISA and nPCR was 0.765. The results indicate that BLV infection spread widely in dairy farms and the nPCR is rapid method for the early detection of BLV infection.
Detection of etiologic agents in diarrhea fecal samples from calves in Gyeongnam province, Korea
Jeong, Myeong-Ho ; Lee, Min-Kwon ; Kim, Hyeong-Su ; Lee, Seong-Uk ; Seong, Min-Ho ; Park, Dong-Yeop ; Hwang, Bo-Won ; Park, Hyoung-Joon ; Cho, Jae-Hyeon ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 339~342
DOI : 10.7853/kjvs.2012.35.4.339
The objective of this study was to determine the infection patterns of etiological agents causing calf diarrhea in the Gyeongnam province, Korea. In this study, from January 2011 to December 2011, feces and necropsy specimens from 249 calves diagnosed with diarrhea (<7 months old) were examined by reverse transcriptase-polymerase chain reaction assay and bacteria & coccidium isolation for detection pathogenic organism. The results of this study showed that 78 cases (31.3%) in spring, 71 cases (28.5) in summer, 62 cases (24.9%) in fall and 38 cases (15.3%) in winter were diagnosed with calf diarrhea, respectively. Calf diarrhea-causing pathogens were diagnosed as bacteria 113 (45.4%), viruses 97 (39.0%), coccidium 1 (0.4%), unknown cases 13 (5.2%), and mixed infections 25 (10.0%). We isolated three virus types from fecal samples (97), which were classified as BVD 64 (66.0%), BRV 21 (21.6%), and BCV 12 (12.4%). Moreover, co-infected pathogens were 25 cases, consisting with BVD & BRV 11 (44%), BVD & BCV& BRV 7 (28.0%), E. coli & BCV 3 (12%), and BVD & IBR 1 (4.0%). In summary, we demonstrated that the enteropathogens of bacteria, viruses, and parasite were detected in samples from cattle with diarrhea, principally in young calves less than 7 months of age. Future studies of infectious diarrhea in cattle should include assays for this etiologic agent.
Effect of feeding the combination with Lactobacillus plantarum and Bacillus subtilis on fecal microflora and diarrhea incidence of Korean native calves
Lee, Yeo-Eun ; Kang, In-Jin ; Yu, Eun-Ah ; Kim, Suk ; Lee, Hu-Jang ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 343~346
DOI : 10.7853/kjvs.2012.35.4.343
This study was investigated the effects of feeding the combination with Lactobacillus plantarum and Bacillus subtilis on the diarrhea incidence and fecal microflora of weaned calves. A total of 12 newly weaned calves were allocated to two dietary treatments in a randomized design based on body weight. The dietary treatments included a commercial basal diet supplemented with: 1) no microbial inoculants (Control); 2) a mixture of Lactobacillus plantarum and Bacillus subtilis (LB). Calves were fed diets for a 4-week period. At the end of the experiment, the counts of fecal lactic acid bacteria and Enterobacteriaceae in LB were significantly improved compared to control (P<0.05). Over the 4-week period, fecal scores and duration of diarrhea in LB were significantly decreased compared with those in control (P<0.05). The present results suggest that LB is a potential feed additive which could be used for the balance of intestinal microflora and the prevention of diarrhea in Korean native calves.
Effect of L-theanine on non-specific immunoparameters in catfish (Silurus asortus)
Heo, Gang-Joon ; Shin, Gee-Wook ;
Korean Journal of Veterinary Service, volume 35, issue 4, 2012, Pages 347~350
DOI : 10.7853/kjvs.2012.35.4.347
L-theanine was examined for its effects on the generation of superoxide anion, lysozyme and anti-protease in the plasma of catfish (Silurus asotus) by a single intraperitoneal injection with five different concentrations (0, 3, 6, 9 and 12 mg/kg). When compared with the mock-injected group (0 mg/kg), both groups injected with 6 and 9 mg/kg were significantly enhanced in levels of superoxide anion in leukocytes, lysozyme and anti-protease in plasma. Based on the results, L-theanine is thought to function as an immunostimulant and/or immunomodulator on non-specific immune responses in catfish.