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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 36, Issue 4 - Dec 2013
Volume 36, Issue 3 - Sep 2013
Volume 36, Issue 2 - Jun 2013
Volume 36, Issue 1 - Mar 2013
Selecting the target year
Molecular identification of the vaccine strain from the inactivated bovine viral diarrhea virus (BVDV) vaccines
Yang, Dong-Kun ; Kim, Ha-Hyun ; Cho, Soo-Dong ; Choi, Sung-Suk ; Kim, Jae-Jo ; Song, Jae-Young ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 1~6
DOI : 10.7853/kjvs.2013.36.1.1
Since the 1980's, several kinds of inactivated bovine viral diarrhea virus (BVDV) vaccines have been used to immunize domestic animals such as cattle and goat in Korea. Immunogenicity of the BVDV vaccines has been checked by the Korean Veterinary Authority using laboratory animals. In this study, we applied a molecular method to investigate the genetic characterization of the BVDV genes in six commercial inactivated BVDV vaccines, and determined the efficiency of two extraction reagents (i.e., sodium citrate or isopropyl myristate) to separate the vaccine antigens from the antigen/adjuvant complexes. Six partial non-coding regions (288 bp) were successfully amplified with specific primer sets, which demonstrated that sodium citrate is more efficient in extracting viral RNA from inactivated gel vaccines than isopropyl myristae. In addition, we identified the virus strains from the vaccines by analyzing the nucleotide sequences of the 5' non-coding region (NCR) of BVDV. The nucleotide similarity of the partial 5' NCR ranged from 95.1 to 100% among BVDV vaccine strains, respectively, indicating that a few manufacturers used different BVDV strains to produce their vaccines.
Prevalence of bovine viral diarrhea virus from dairy cattle farms in Gyeongnam southern area, Korea
Park, Jong-Sik ; Park, Jong-Kyu ; Cho, Eun-Jung ; Kim, Eun-Gyeong ; Lee, Jong-Min ; Kim, Do-Kyung ; Son, Seong-Ki ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 7~13
DOI : 10.7853/kjvs.2013.36.1.7
Bovine viral diarrhea virus (BVDV) is one of the most important disease viruses in cattle that can cause severe economical losses due to decreased fertility, abortion, diarrhea, and respiratory symptoms. Therefore, this study was aimed to investigate prevalence of BVDV infection (Transiently infection, Persistently infection) in dairy cattle in Gyeongnam southern area, Korea and use this data as the basis for establishing an eradication program and policy. A total of 44 bulk-tank milk samples (farms) collected in milk collecting center were tested for BVDV antibody using an ELISA. As the result, out of a total of 44 bulk-tank milk samples (farms), 38 (86.4%) samples were BVDV antibody positive. Blood samples (17 farms, n=543) were collected from BVDV antibody positive farms in bulk-tank milk, tested for BVDV antigen with ELISA and PCR. BVDV infected farms were 47% (8/17) and BVDV infected head were 2.2% (12/543). Persistently infected cattle (PI) were detected at 6 (35.3%) farms out of 17 farms and a total of 6 (1.1%) out of 543 head of cattle were identified as PI. The seropositive of BVDV antibody at farms and head were 100% (17/17) and 49.45% (91/184), respectively. The seroprevalence of BVDV antibody in PI infected farms (67.35%) much higher than that of BVDV antibody in transiently infected cattle (TI) infected farms (45%) and uninfected farms (34.48%). For eradication of BVDV infection in cattle populations, First of all, we should remove PI and need vaccination.
Development of antibodies after foot and mouth disease vaccination in pigs
Ahn, Gil-Ho ; Bae, Jin-Gyu ; Jung, Kwang ; Wang, Young-Il ; Jung, Jun-Yong ; Kang, Soon-Keun ; Kwon, Hyuk-Moo ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 15~21
DOI : 10.7853/kjvs.2013.36.1.15
Three serotypes (O+A+Asia1 type) of the foot-and-mouth disease (FMD) vaccine were injected into cloven-hoofed animals in Korea after the nationwide spread of FMD at the end of 2010. This study was conducted to investigate FMD antibody development after FMD vaccination, and to determine whether there was a significant correlation between the antibody titer of the sow and the antibody titer of the growing pigs. The antibody titer (percentage inhibition [PI] titer) of the sow (gilt) after FMD vaccination was maintained at a level higher than 50 (P<0.05) for 5 months. A higher PI titer for the 1-month-old growing pigs corresponded with greater inhibition of the PI titer of the vaccinated growing pigs (P<0.05). A negative correlation (P<0.05) between the PI titer of the 1-month-old growing pigs and the PI titer of 3-month-old growing pigs, 4-month-old growing pigs after FMD vaccination at 2 months, 3months was identified, with a coefficient of determination (
) of 0.274. Thus the PI titer of the growing pigs was inhibited to a greater degree when vaccination was performed at 2 months of age than at 3 months. However, many other factors likely influence growing pigs' PI titer in addition to the PI titer of the sow and age at vaccination, given that the coefficient of determination was somewhat lower.
Molecular characterization of avian rotavirus isolated in Korea
Wang, Jun-Hui ; Koo, Bon-Sang ; Mo, In-Pil ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 23~30
DOI : 10.7853/kjvs.2013.36.1.23
An avian rotavirus (AvRV-2) was isolated from feces of broilers suffering from acute gastroenteritis in 2011. It was the first avian rotavirus isolated in Korea. To investigate the molecular characteristics of AvRV-2, the VP4, VP6, VP7 and NSP4 gene nucleotide sequences were determined and compared with those of rotavirus strains available in the GenBank database. The phylogenetic tree of VP7 gene showed that AvRV-2 had a high degree of nucleotide sequence homology (93.4% to 94.7%) with those of rotaviruses belonging to genotype G19 cluster. The phylogenetic tree of the VP4 gene revealed a high degree of nucleotide sequence homology (95.8% to 95.9%) with genotype P rotaviruses isolated from chickens. The VP6 and NSP4 gene nucleotide sequences showed the highest identities with those of avian strains with 95.3% to 96.4% and 90.3% to 92.2%, respectively. Genetic characterization of the VP4, VP6, VP7 and NSP4 showed that AvRV-2 strain was most closely related to chicken rotavirus strains from Germany and Japan. Comparative nucleotide sequences and phylogenetic analysis indicated that avian rotavirus isolated from broilers belonged to genotype G19P and it was the first report on avian rotavirus infection in Korea.
Prevalence of Johne's disease from slaughtered cattle in central area of Gyeongnam province, Korea
Son, Byeong-Guk ; Seok, Ju-Myoung ; Jang, Eun-Hee ; Ji, Dae-Hae ; Shin, Jeong-Seop ; Hwang, Bo-Won ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 31~36
DOI : 10.7853/kjvs.2013.36.1.31
Mycobacterium avium subsp. paratuberculosis is the pathogen of paratuberculosis called Johne's disease. Johne's disease is hardly eliminated because of its long latent period and continuous dissemination, so it is found in ruminants worldwide and can cause substantial economic losses in cattle. It has been reported in many studies on the distribution of Johne's disease in some provinces of Korea that not many, but noticeable numbers of infected cows have been detected since the first detection in 1984. The aims of this study was to investigate the prevalence of Johne's disease obtained from slaughtered cattle in central area of Gyeongnam province, Korea. In this study, the ELISA serum antibody test and PCR were employed on a total of 240 blood and ileac substrate samples from slaughtered cattle in two slaughtering and wholesale centers in Gyeongsangnam-do Livestock Veterinary Research Institute Central Branch. Out of the entire 240 blood samples, three (1.3%) were positive by ELISA, while five (2.1%) were suspected cattle. But ileac substrate samples, eight (3.3%) were positive by PCR. By breeds, positive rates of ELISA and PCR in Korean native cattle were 1.3% and 3.5%, respectively, but no positive cows were found in dairy cattle. By provinces, sero-positive rates of Gyeongnam and Gyeongbuk were 1.6% and 1.3%, respectively. And PCR positive rates of Gyeongnam, Gyeongbuk and other provinces were 2.4%, 5.0% and 2.8%, respectively. These results indicate that it requires the nationwide monitoring test and measure to deal with subclinically infected slaughtering cows.
Molecular detection of infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province, Korea
Ouh, In-Ohk ; Do, Jae-Cheul ; Seo, Min-Goo ; Jeong, Tae-Nam ; Cho, Min-Hee ; Kwak, Dong-Mi ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 37~44
DOI : 10.7853/kjvs.2013.36.1.37
The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010~2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.
Hepatoprotective effect of Schisandra chinensis on high-fat diet-induced fatty liver in rats
Song, Yun-O ; Lee, Soo-Jung ; Park, Hyoung-Joon ; Jang, Sun-Hee ; Chung, Byung-Yeoup ; Song, Young-Min ; Kim, Gon-Sup ; Cho, Jae-Hyeon ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 45~52
DOI : 10.7853/kjvs.2013.36.1.45
The purpose of the present study is to determine whether Schisandra chinensis (SC) has a protective effect on high fat diet (HFD)-induced fatty liver including hepatic lipid accumulation in rats. The HFD-induced obese rats were weighed after SC extracts were administered through the gastrointestinal tract at a concentration of 250 mg/kg b.w/day for 5 weeks. After 5 weeks, all of the rats on a high fat-diet were 36.5% heavier compared with normal controls. In contrast, rats on a high-fat diet supplemented with SC were 23.5% lighter than rats fed only a high-fat diet. Although there was no significant difference in food intake among the groups during the experimental diet period, the body weight gain of the SC group was significantly lower than the weights of the HFD groups. SC treatment slightly decreased the liver weight. Reduction of hepatic TBARS contents by SC was observed in rats fed a diet containing SC, and antioxidant activity was markedly increased in HFD＋SC group compared to those of HFD group in liver. Moreover, total-lipid and triglyceride contents in the liver of groups fed a diet containing SC were significantly lower compared to those of the HFD group. High fat feeding elevated liver cholesterol concentration, but the addition of SC to the HFD rats resulted in the significant decrease in liver cholesterol. In histological observation of liver tissues, the hepatocytes of HFD rats showed a typical fatty liver morphology showing numerous lipid droplets in cytoplasm, whereas administration of SC reduced the size and numbers of lipid droplets. These results clearly demonstrated the attenuation of SC on nonalcoholic fatty liver induced by obese rats fed HFD.
Seroprevalence of Neospora caninum in dairy cattle of northern Gyeonggi province in Korea
Jung, Kwang ; Shim, Hang-Sub ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 53~56
DOI : 10.7853/kjvs.2013.36.1.53
This study was carried out to investigate the seroprevalence of Neospora caninum infection in dairy cattle of northern Gyeonggi province in Korea. A total of 716 dairy cattle from 14 dairy farms were tested for the presence of antibodies against N. caninum using a commercial ELISA test kit. Herd and individual seroprevalences of N. caninum were 92.8% (13/14) and 23.1% (166/716), respectively. The seroprevalence of dairy cattle according to age was the highest at 49~72 months (27.4%), but the difference was not statistically significant (P>0.05). No significant relationship between N. caninum seropositivity with the herd size of the dairy farms was found (P>0.05). The results indicate that N. caninum infection spreads widely in dairy farms of northern Gyeonggi province in Korea.
Prevalence and antimicrobial resistance patterns of Campylobacter jejuni from duck feces
Kim, Neung-Hee ; Chae, Hee-Sun ; Kang, Yong-Il ; Shin, Bang-Woo ; Choi, Nong-Hoon ; Kim, Hyo-Bi ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 57~60
DOI : 10.7853/kjvs.2013.36.1.57
This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.
A study of infectious duck diseases in western Gyeongnam province, Korea
Kim, Min-Hee ; Jo, Myeong-Hui ; Ryou, Seong-Hwan ; Bang, Sang-Young ; Heo, Jeong-Ho ; Kim, Hyun-Joon ;
Korean Journal of Veterinary Service, volume 36, issue 1, 2013, Pages 61~65
DOI : 10.7853/kjvs.2013.36.1.61
We investigated the prevalence of infectious duck diseases using 156 ducks reared in 18 farms of western Gyeong-nam province. As a result, duck viral hepatitis (12.8%), colibacillosis (7.1%), and fungal disease (9.0%) were detected. However, avian influenza and riemerellosis were not detected. During autopsies, we could grossly observed red swollen liver (12.8%), petechial or ecchymotic hemorrhage on liver (11.5%), and fibrinous perihepatitis (9.0%). Gray-white necrotic spot (23.1%), swollen spleen (22.8%), swollen kidney (20.5%), hyperemia or hemorrhage on tracheal mucous membrane (8.3%), and nodule in long or air sac (9.0%) were also found.