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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
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Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 36, Issue 4 - Dec 2013
Volume 36, Issue 3 - Sep 2013
Volume 36, Issue 2 - Jun 2013
Volume 36, Issue 1 - Mar 2013
Selecting the target year
Comparison of the pathogenicity among Cronobacter species in a neonatal mouse model
Hong, Sun-Hwa ; Chung, Yung-Ho ; Park, Sang-Ho ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 67~71
DOI : 10.7853/kjvs.2013.36.2.67
Neonatal infection caused by Cronobacter species can result in serious illnesses such as bacteremia, septicemia, meningitis, and death in at-risk infants who are orally fed contaminated reconstituted powdered infant formulas. The objective of this study was to compare the virulence among three Cronobacter species strains by using an animal model for human neonatal Cronobacter species infections. We acquired timed-pregnant ICR mice and all owed them to give birth naturally. On postnatal day 3, each pup was administered orally a total dose of
CFU Cronobacter species strain 3439, CDC 1123-79, and 3231. Mice were observed twice daily for morbidity and mortality. At postnatal day 10, the remaining pups were euthanized, and brain, liver, and cecum were excised and analyzed for the presence of Cronobacter species. Cronobacter species were isolated from cecum and other tissues in inoculated mice. In the tissues of Cronobacter species infected mice, meningitis and gliosis were detected in the brain. In this study, we identified the virulence among Cronobacter species strains by using a neonatal mice model which was a very effective animal model for human neonatal Cronobacter species infections.
Analysis of the antibiotic resistance gene in Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province
Kim, Joo-Hyung ; Kim, Seong-Guk ; Kim, Seon-Soo ; Kim, Jeong-Hwa ; Park, Sye-Hee ; Nam, Ki-Hu ; Kim, Hyoung-Bae ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 73~78
DOI : 10.7853/kjvs.2013.36.2.73
This study was conducted to investigate antibiotic resistance among Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province during the period 1998~2011. One hundred forty one isolates were tested for antibiotic resistance using the standard disk diffusion method and were examined for presence of resistance gene by PCR method. S. Typhimurium showed high drug resistance rates to tetracycline (95.7%), streptomycin (93.6%), ampicillin (86.5%), cephalothin (80.1%), gentamicin (79.4%), and trimethoprim/sulfamethoxazole (72.3%). Resistance gene, blaTEM, blaPSE1, tetA, tetB, tetG, sul1, sul2, aadA, strA, grm, and temA were detected among the antibiotic resistance isolates and temB, tetC, aadB gene were not detected. One hundred twenty one (89.6%) tetA, two (1.5%) tetB and one (0.7%) tetG gene were detected in the 135 tetracycline resistant isolates. Two (1.6%) temA gene were detected in one hundred twenty two ampicillin resistance isolates and temB was not detected.
Loop-mediated isothermal amplification assay for differentiation of Mycobacterium bovis and M. tuberculosis
Koh, Ba-Ra-Da ; Kim, Jae-Myung ; Sung, Chang-Min ; Ji, Tae-Kyung ; Na, Ho-Myung ; Park, Seong-Do ; Kim, Yong-Hwan ; Kim, Eun-Sun ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 79~86
DOI : 10.7853/kjvs.2013.36.2.79
Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10
. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.
Antimicrobial and antiviral activity of Saururus chinensis extract by n-Hexane
Lee, Ju-Hyun ; Choe, Yeong-Ho ; Park, Yoon-Jin ; Zhang, Xiao-Wan ; Kim, Byeong-Soo ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 87~93
DOI : 10.7853/kjvs.2013.36.2.87
This study was conducted to investigate the antimicrobial, antivirus properties of Saururus chinensis extracts. The n-hexane extracts from Saururus chinensis showed the active antimicrobial activity against gram-positive bacteria. Minimum inhibitory concentration (MIC) of Saururus chiensis n-hexane extracts was 1.25 mg/ml against B. subtilis and 2.5 mg/ml against S. aureus. The cytotoxicity effects on MDBK (Madin-Darby bovine kidney) cell were observed at the various n-hexane extract concentrations. In
assay, 0.6 mg/ml of n-hexane extracts decreased BVD (bovine viral diarrhea) virus by 1.4 log, whereas other extracts did not show antiviral activity. In this study, The results suggested that n-hexane extracts and fractions of Saururus chinensis can be a candidate materal of feed additive to chemical antibiotics and antivirus substances.
The comparison of microbiological control level between HACCP-accredited ice cream manufacturers and non-accredited ones in the southern part of Gyeonggi province, Korea
Bae, Jin-Gyu ; Jeon, Oh-Sook ; Park, Hye-Won ; Bok, Min-Soon ; Yang, Ho-Yul ; Bark, Jun-Jo ; Ko, Tae-Oh ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 95~103
DOI : 10.7853/kjvs.2013.36.2.95
The hazard analysis critical control point (HACCP) system was introduced to the Korean livestock slaughter and product processing business in 1997. Ice cream manufacturers focus on the sterilization process in order to reduce microorganism, so the HACCP-accredited ice cream manufacturers particularly set the sterilization process as critical control point (CCP). Therefore, the microbiological test results on the finished products can be considered as one of the indicators which show the level of microbiological control in the ice cream manufacturers. Accordingly, this study was carried out to examine if there is a meaningful difference in terms of the microbiological control level between the HACCP-accredited and non-accredited ice cream manufacturer groups by comparing aerobic plate count (APC) of ice creams. The mean of APC was 1.68 Log CFU/g in the HACCP-accredited ice cream manufacturer group and 2.59 Log CFU/g in the non-accredited ones respectively and there was a statistically significant difference (P<0.05). The test results suggest that the level of microbiological control in the HACCP-accredited ice cream manufactures is much higher than that of non-accredited ones.
Prevalence for persistently infected cattle with bovine viral diarrhea virus in Korea
Cho, Jong-Suk ; Kim, Gyung-Dong ; Park, Hong-Je ; Lim, Yeoun-Su ; Hong, Sung-Hee ; Seo, Chang-Won ; Ryu, Hee-Jeong ; Sin, Ryeong-Ja ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 105~110
DOI : 10.7853/kjvs.2013.36.2.105
Bovine viral diarrhea virus (BVDV) is very important disease in domestic and wild ruminants and has a world wide distribution. Cattle persistently infected with BVDV (BVDV-PI) are the primary reservoir for BVDV infection in Korean native cattle herds. The prevalence of cattle persistently infected with BVDV (BVD-PI) was determined using 4,260 heads from 29 Korean native cattle farms at 8 districts from 2011 to 2012. The sera and ear nothches were collected for each sample. We surveyed BVD-PI cattle using antibody ELISA and antigen capture ELISA for detection of antibody and antigen respectively. Three thousand seventy-six cattle (72.2%) were positive for BVDV antibody and a total of 27 BVD-PI cattle were found in 12 farms. 11 cattle (40.7%) out of the total 27 BVDV-PI cattle were six months old or under. The positive rate of BVDV antibody (83.2%) from 12 farms with BVD-PI cattle was higher than the positive rate of BVDV antibody (63.6%) from 17 farms without BVD-PI cattle.
Isolation frequency and antimicrobial resistance of Escherichia coli & Enterococcus spp. isolated from beef & pork on sale in Seoul, Korea
Kim, Ju-Young ; Park, Mi-Ae ; Kim, Jee-Eun ; Chae, Hee-Sun ; Park, Yeon-Jae ; Son, Jang-Won ; Yang, Yun-Mo ; Choi, Tae-Seok ; Lee, Ju-Hyung ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 111~119
DOI : 10.7853/kjvs.2013.36.2.111
The aim of this study was to investigate the isolation frequency and antimicrobial resistance of Escherichia coli (E. coli) and Enterococcus spp. from domestic beef and pork on sale in Seoul, Korea. A total of 106 (10.4%) E. coli and 114 (11.2%) Enterococcus spp. from 635 domestic beef and 381 pork samples were isolated and examined for susceptibility to 15 and 11 antimicrobial agents, respectively. The most frequent antimicrobial resistance observed in E. coli isolates was to ampicillin (38.6%), followed by streptocmycin (34.9%) and tetracycline (32.0%). The most frequent antimicrobial resistance in E. faecium isolates was to erythromycin (53.8%) and rifampin (46.1%), and in E. faecalis isolates was to tetracycline (55.7%) and rifampin (55.0%). Among the isolates, multi-drug resistant (MDR) E. coli and Enterococcus spp. strains showing resistances to more than two antimicrobial agents tested were 10.4% and 11.2%, respectively. As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in future.
A survey of the relation between fatty lesions after slaughter inspection results and cattle carcasses quality grades in Korea
Sung, Chang-Min ; Ji, Tae-Kyung ; Choi, Jong-Wook ; Park, Da-Hae ; Park, Dae-Hee ; Kim, Hyun-Joong ; Kim, Yong-Hwan ; Kim, Eun-Sun ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 121~125
DOI : 10.7853/kjvs.2013.36.2.121
The survey was conducted to investigate the relation between slaughter inspection results and the quality of cattle carcasses by inquiring animal products grading service results of Korea Institute for Animal Products Quality Evaluation (KAPE) about partial-disposed cattle carcasses after dismantling inspection. Of the 13,586 slaughtered cattle, 1,343 (9.9%) cattle heads were partially disposed from January to August, 2012 at slaughterhouses in Gwangju area, Korea. Of the 1,343 partial disposed cattle, 613 (45.6%) cattle carcasses were graded into above "Grade 1". Among partially disposed 1,171 Hanwoo heads, 613 (52.3%) cattle carcasses were graded into above "Grade 1". However, none of dairy and beef cattle carcasses were graded into above "Grade 1" out of 163 and 9 partial disposals, respectively. Of the 221 partial disposals by enteric fat necrosis, 139 carcasses were graded into above "Grade 1", and the ratio; 62.9% (P
A case of canine bilateral ovary granulosa cell tumor and mammary complex carcinoma
Chung, Yung-Ho ; Hong, Sunhwa ; Han, Sang-Jun ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 127~132
DOI : 10.7853/kjvs.2013.36.2.127
An 11-year-old poodle bitch was presented for investigation of multicentric mammary masses. Abdominal sonography and radiography demonstrated abnormal enlargement of uterus and ovaries. Blood analysis revealed high progesterone concentration. The ovariohysterectomy and mastectomy were performed. Histopathologically, the mammary masses revealed complex carcinoma-tubulopapillary carcinoma with papillary pattern and tubule pattern. In the uterus, cystic endometrial hyperplasia was observed. Scattered inflammatory cells were observed in the endometrial stroma and mucinous material was protruded from endometrial surface. Also, in the ovaries, bilateral ovary granulosa cell tumor was detected. The bitch made a complete recovery following the ovariohysterectomy and mastectomy. This case was a very rare multiple tumor occurrence with bilateral ovary granulosa cell tumor and mammary complex carcinoma. High progesterone concentration was characterized clinically in the bitch.
Seroprevalence of paratuberculosis in Korean cattle in western Jeonbuk area, Korea
Chu, Keum-Suk ; Kim, Sang-Hun ; Ha, Yong-Su ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 133~137
DOI : 10.7853/kjvs.2013.36.2.133
The purpose of this study was to investigate the seroprevalence of paratuberculosis in Korean cattle in western Jeonbuk area. From February 2012 to January 2013, blood samples were collected from 2,606 Korean cattle of 263 farms. As a result, 60 (2.3%) heads of 46 (17.5%) farms were positive on the ELISA test for paratuberculosis. Based on regional analysis, 18 (19.6%) out of 92 farms and 24 (2.5%) out of 941 heads in Iksan area, 28 (22.0%) out of 127 farms and 36 (2.8%) out of 1,291 heads in Kimje area were positive but samples from Gunsan area were all negative. According to scale breeding, small scale (below 50 heads) breeding showed the most high prevalence rate compared to middle (50 to 99 heads) or large (over 100 heads) scale breeding. To clarify the relation between number of heads and paratuberculosis prevalence, some additional analysis would be required in further, though.
Study on porcine respiratory disease complex from slaughtered pigs in Namwon, Korea
Kang, Mi-Seon ; Kang, Min-Woo ; Jung, Se-Ho ; Lee, Hee-Seon ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 139~145
DOI : 10.7853/kjvs.2013.36.2.139
Porcine respiratory disease complex (PRDC) continues to be a significant economic problem to the swine industry. In order to elucidate the etiology of PRDC including porcine circovirus type 2 (PCV2), porcine reproductive and respiratory disease syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (MH), Pasteurella multocida (PM) and Actinobacillus pleuropneumoniae (APP) in Namwon, the 455 lung samples were randomly collected from slaughtered pigs, examined gross lesions indicative of respiratory disease of lung and classified the lung lesion according to the severity of lung lesions. Two hundred pigs lung tissues with pneumonic lesions were examined for pathogen by PCR. As a result, the numbers of pneumonic lesions were 357 (78.5%), mean pneumonic score (
and the highest gross lesion according to stages was 1 (11~20%). In detection of pathogens, PCV2, PRRSV, SIV, MH, APP and PM were positive in 76.5%, 5.0%, 6.0%, 9.0%, 4.5% and 6.0%, respectively and PCV2-MH was the most detected causative pathogens of PRDC in co-infection. In the serological test for PRRSV, PCV2, MH, APP2, APP5, HP and PM, showed high antibody positive rates 93% or more.
Prevalence of honeybee (Apis mellifera) disease in Cheonan-Asan areas, Korea
Jeon, Dong-Min ; Kim, Sun-Hee ; Yook, Sim-Yong ; Yeam, Nam-Hee ; Do, Jin-Young ; Song, Seo-Young ; Heo, Eun-Jin ; Sin, Chang-Ho ;
Korean Journal of Veterinary Service, volume 36, issue 2, 2013, Pages 147~150
DOI : 10.7853/kjvs.2013.36.2.147
This study was carried out to investigate the prevalence of honeybee (Apis mellifera) disease in cheonan and asan area. From September to November in 2012, 33 samples were collected from 33 apiculture farms in the regions and reverse transcriptase-polymerase chain reaction (RT-PCR) and polymerase chain reaction (PCR) was conducted. Among 33 samples, prevalence rate was 42% in Sac Brood Virus (SBV), 52% in Nosema, 21% in American foulbrood (AFB), 70% in European foulbrood (EFB), 97% in Stonebrood, 3% in Chalkbrood. The result indicate that stonebrood was most prevalent disease in apiculture farms in cheonan and asan area.