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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 36, Issue 4 - Dec 2013
Volume 36, Issue 3 - Sep 2013
Volume 36, Issue 2 - Jun 2013
Volume 36, Issue 1 - Mar 2013
Selecting the target year
The follow up study after massive outbreak of Akabane and bovine ephemeral fever viruses in Korea
Yang, Dong-Kun ; Kim, Sun-Young ; Kim, Ha-Hyun ; Kang, Mi-Sun ; Nah, Jin-Ju ; Choi, Sung-Suk ; Seok, Kum-Ok ; Cho, Jong-Suk ; Song, Jae-Young ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 151~155
DOI : 10.7853/kjvs.2013.36.3.151
Since a large number of Akabane and bovine ephemeral fever (BEF) infection occurred in the southern part of Korea in 2010, recent information about seroprevalence of Akabane virus (AKAV) and bovine ephemeral fever virus (BEFV) has been required for preventing both diseases. In this study, serological assay against AKAV and BEFV using virus neutralization assay was conducted using 1,743 bovine sera collected from Namwon, Miryang, Yeongju and Uljin which located in Southern part of Korea from March to May in 2012. The overall seropositive rates for AKAV and BEFV were found to be 49.8% and 1.2%, respectively. The regional distribution of seroprevalence for AKAV ranged from 18.1% to 63.7%. Seroprevalences of AKAV were 63.7% in Miryang, 62.3% in Uljin, 50.7% in Namwon, and 18.1% in Yeongju. The seropositive rates for AKAV in southern part of Korea were higher than the annual average at the national level. On the other hand, seropositive rates of BEFV in four regions were from 0.3 to 3.1%. In detail, regional seroprevalences were 3.1% in Miryang, 2.0% in Uljin, and 1.7% in Yeongju, and 0.3% in Namwon. Even only one year after massive outbreaks, overall seropositive rates were very low, similar to the annual average at the nation level. This result indicates that many number of cattle infected with BEFV may be replaced by new born calf or cattle in farm may not be immunized with vaccines. To prevent another epidemic, a national wide warning should be issued and more aggressive control measure must be implied. Recent global warming phenomenon could lead to more vigorous activity of haematophagous vectors and it is possible that arboviral diseases such as AKAV and BEFV are increased. Therefore, continuous sero-monitoring and extensive vaccination combined with control of haematophagous vectors are important to effectively prevent and control diseases caused by AKAV and BEFV.
Effects of ozone, ultraviolet and an organic acid-based disinfectant against porcine reproductive and respiratory syndrome virus
Yoon, Yong-Dae ; Kim, Won-Il ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 157~162
DOI : 10.7853/kjvs.2013.36.3.157
A good level of biosecurity practice is important for efficient porcine reproductive and respiratory syndrome (PRRS) control. In the current study, disinfecting ability of ozone against PRRS virus (PRRSV) was evaluated in comparison with ultraviolet (UV) and an organic acid-based disinfectant to assess the possible use of ozone for disinfecting farm vehicles, equipments, and materials to reduce the risk of new virus introduction. For in vitro evaluation, the levels of infectious virus and viral RNA were determined on the swabs collected from the floor surface of each room treated with either ozone, UV or the disinfectant up to 30 min after contamination with 100 mL of VR2332 (
). For in vivo evaluation, 3, 4-week old, PRRS-free pigs were housed into those rooms right after the last swab collection. Then the pigs in each room were injected intramuscularly with the corresponding swab samples collected at the last time point and pooled per each room. Although ozone, UV, and the disinfectant significantly reduced the levels of PRRSV RNA contamination, ozone was most effective in removing the viral RNA. In addition, the virus collected after at least 10 min exposure to ozone failed to replicate in pigs while the virus collected after treatment with UV and the disinfectant for 30 min still replicated in pigs. Based on the results, it was concluded that ozone is more effective in inactivating PRRSV as compared with UV and the organic acid-based disinfectant.
Clinical and pathological studies on co-infection of lowpathogenic avian influenza virus and Newcastle disease virus in the chicken
Lee, Sung-Min ; Cho, Eun-Sang ; Choi, Bo-Hyun ; Son, Hwa-Young ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 163~169
DOI : 10.7853/kjvs.2013.36.3.163
Both of avian influenza (AI) and Newcastle disease (ND) can cause mild to severe diease in poultry. In this study, clinical signs, macro, and micro lesions were studied. Eighteen six-week-old SPF chicks were divided into 4 groups (E1, E2, E3 and C1) and housed in different rooms of the isolation facility at CAVAC (Daejeon, Korea). The control group (C1) of 3 chicks was housed separately as uninoculated. Experimental groups (E1, E2 and E3) challenged with H9N2 and/or NDV. E1 group was challenged with 0.1 mL A/Kr/Ck/01310/01 (H9N2)
by intranasal, E2 group was challenged with 0.5 mL Kyojeongwon (KJW)
by intramuscular, and E3 group was challenged with 0.1 mL A/Kr/Ck/01310/01
by intranasal and 0.5 mL KJW
by intramuscular 7 days after H9N2 challenge. In clinical signs and gross findings, E1 group showed 0% mortality, anorexia, and hemorrhage of proventriculus and thymus, E2 group showed 100% mortality within 3~5 days after challenge, anorexia, green diarrhea, hemorrhage of proventriculus, proximal esophagus and thymus, enlargement of kidney, and bronze liver, and E3 group showed 100% mortality within 24~36 hours after NDV challenge, depression, anorexia, green diarrhea, hemorrhage of proventriculus, spleen, and lung, enlargement of kidney, and reduction of thymus size and number. In histopathological examination, E1 group showed depletion and necrosis in bursa of Fabricius, thymus, and spleen, and E2 and E3 group showed severe lymphocyte depletion and necrosis with destruction of lymphoid organ structures. In conclusion, co-infection of H9N2 with ND virus causes acute disease with high mortality than single infection and the pathologic lesions were more severe.
Antimicrobial resistance and distribution of resistance gene in Enterobacteriaceae and Pseudomonas aeruginosa isolated from dogs and cats
Cho, Jae-Keun ; Kim, Jin-Hyun ; Kim, Jeong-Mi ; Park, Choi-Kyu ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 171~180
DOI : 10.7853/kjvs.2013.36.3.171
This study was carried out to investigate the antimicrobial resistance pattern and distribution of resistance gene in 44 Enterobacteriaceae and 21 Pseudomonas (P) aeruginosa isolated from hospitalized dogs and cats in animal hospital from 2010 to 2011 in Daegu. Among Enterobacteriaceae, Escherichia (E) coli was highly resistant to ampicillin (56.7%), followed by tetracycline (53.3%), cephalothin, streptomycine, sulfamethoxazole/trimethoprim, gentamicin and norfloxacin (40.0~43.3%). The remaining isolates of Enterobacteriaceae had high resistance to ampicillin (64.3%) and streptomycin (42.9%). Whereas, P. aeruginosa was low resistant to all antimicrobials tested (less than 15%). int I 1 gene was detected in 20 (57.1%) of 35 antimicrobial resistant Enterobacteriaceae and 2 (9.5%) of 21 P. aeruginosa., but int I 2 gene was not detected in all isolates. The eight resistance genes were found either alone or combination with other gene (s):
, aadA, strA-strB, clmA, tetA, tetB, sul I and sul II. About 78% of integron-positive isolates were resistance to more than four antimicrobial agents. The findings suggest that class I integrons are widely distributed in E. coli among Enterobacteriaceae from dogs and cats and multi-drug resistance related to the presence of class I integrons. The prudent use of antimicrobials and continuous monitoring for companion animals are required.
Isolation, identification and serological investigation of Actinobacillus pleuropneumoniae in slaughtered pigs
Kim, Kyung-Eon ; Ku, Kyung-Nyer ; Ko, Jae-Hyung ; Moon, Hyeong-Jun ; Choi, Kwon-Rag ; Song, Eun-Ah ; Park, Mi-Young ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 181~186
DOI : 10.7853/kjvs.2013.36.3.181
This study was conducted to isolate the Actinobacillus pleuropneumoniae (APP) and to find out the distribution of 15 serovars mainly in southern Gyeonggi province, Korea. From July 2011 to Nov. 2012, a total of 2,204 slaughter pigs (110 herds) were inspected for evaluation of APP like pneumonic lesions. 48 (33.8%) APP strains were isolated from the 142 lungs and identified using PCR assays (cps, apx/omlA, biovar). Consequently, the serotype ratio were as in the following; type2 41.7% (n=20), type5 33.3% (n=16), type12 10.4% (n=5), type1 6.2% (n=3), type4 and 7 2.1% (n=1) and unknown 4.2% (n=2). Also serological test was implemented for 452 (83 herds) serum samples randomly collected from above slaughter pigs using commercial ELISA kits. The positive ratio of each serotype for tested pigs were 19.1% (77/404) on , 7.1% (32/452) on [3, 6, 8], 6.9% (28/404) on [5a, 5b], 6.2% (28/452) on [4, 7], 2.8% (9/320) on , 2.0% (9/452) on [1, 9, 11] and 0.0% (0/452) on . And 49.3% (223/452) of pigs were positive on apxIV antibody. On the basis of latter screening test, the infected farm ratio accounted for 71.1% (59/83) and that was much higher than previously reported data.
Prediction survey on the viral diseases of companion animals in Gwangju area, Korea
Na, Ho-Myung ; Bae, Seong-Yeol ; Lee, Yeun-Ey ; Park, Jae-Sung ; Park, Seong-Do ; Kim, Eun-Sun ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 187~192
DOI : 10.7853/kjvs.2013.36.3.187
For the monitoring of six viral disease (CIV: canine influenzavirus, CPIV: canine parainfluenzavirus, CHV: canine herpesvirus, CPV2: canine parvovirus type 2, CCoV: canine coronavirus, CNV: canine norovirus) inspections, a total of 300 samples were collected nasal or feces from the companion dogs of animal hospital (n=98) and the abandoned dogs of animal shelters (n=202) in Gwangju, Korea. Using PCR and RT-PCR, CPV2, CPIV and CHV were detected in 55 (18.3%), 11 (3.7%), 1 (0.3%), respectively. CPV2 was highly detected in May, October and November. and CPIV was highly detected in November. But those agents were not detected the virus in March and July. Based on the results of the investigation continuous monitoring for companion and abandoned dogs will be required.
Application of the TaqMan
real-time PCR assay for the detection of chicken (Gallus gallus) meat in pork products
Koh, Ba-Ra-Da ; Kim, Ji-Yeon ; Na, Ho-Myung ; Park, Seong-Do ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 193~201
DOI : 10.7853/kjvs.2013.36.3.193
Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqMan
quantitative real-time polymerase chain reaction (PCR) assay was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient (
) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not contaminated with chicken residuals. Only twelve pork products of one of the manufacturers were in accordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consumers' trust.
A survey of the microbial contamination level in butcher's shops in Seoul, Korea
Yang, Yoon-Mo ; Son, Jang-Won ; Choi, Tae-Seok ; Park, Mi-Ae ; Kim, Ju-Young ; Lee, Joo-Hyung ; Shin, Bang-Woo ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 203~208
DOI : 10.7853/kjvs.2013.36.3.203
This survey was conducted to evaluate the microbial contamination level of butcher's shops in Seoul, Korea. For microbial inspections, a total of 584 samples (146 cotton work gloves, 146 utensils and equipments, 154 beef samples, 138 pork samples) were collected from butcher's shops. E. coli and pathogenic bacteria such as Staphylococcus aureus, Yersinia enterocolitica, Listeria monocytogenes, Salmonella spp., and E. coli O157:H7 were tested in the samples. As a result, the level of aerobic plate count (APC) ranged
from utensils and equipments,
CFU/glove from cotton work gloves and
CFU/g from meat. The APC level of E. coli ranged
from utensils and equipments,
CFU/glove from cotton work gloves, and
CFU/g from meat, respectively. Staphylococcus aureus was detected in 2 beef samples, 1 pork sample, and 10 used cotton work gloves. Yersinia enterocolitica was detected in 3 beef samples, 1 pork sample, and 3 used cotton work gloves. Listeria monocytogenes was detected in 2 used cotton work gloves. In order to improve the sanitation status of butcher's shops, application of HACCP (Hazard Analysis Critical Control Point) or SSOP (Sanitation Standard Operating Procedure), regular hygiene education, and continuous monitoring for microorganisms will be required.
A survey on hygiene management for raw by-products of beef in Gwangju area, Korea
Kim, Ji-Yeon ; Jang, Mi-Sun ; Koh, Ba-Ra-Da ; Ji, Tea-Kyung ; Sung, Chang-Min ; Park, Da-Hae ; Kim, Hyun-Joong ; Kim, Eun-Sun ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 209~216
DOI : 10.7853/kjvs.2013.36.3.209
A total of 301 samples of bovine liver, spleen and omasum were collected from butchers and restaurants in Gwangju, Korea during 2012 and all samples were subjected to bacteriological examination and antibiotic residues. Also, this study was performed to survey the consciousness for hygiene of livestock workers who are handling bovine by-products in Gwangju. The detection rate of aerobic plate count (APC) was higher in summer than in other seasons in all by-products (P=0.000). The detection rate of E. coli count was lower in the liver than the spleen and omasum (P=0.000). Twenty four of the samples (8.0%) were contaminated with S. aureus while one spleen sample (0.3%) was contaminated with L. monocytogenes and finally 10 (3.3%) of the liver and omasum samples were contaminated with Cl. perfringens. Five of the twenty-four S. aureus isolates harbored enterotoxin gene. However, the cpe gene of Cl. perfingens was not detected among any of the 10 isolates. Antibiotic residues were not detected in the liver samples. The consciousness survey's results showed that most of them (58.8%) were safe.
Survey of calcium, fat and lactose contents in processed milks
Jeon, Hae-Chang ; Lee, Kyung-Hye ; Han, Hye-Jin ; Yun, Min ; Kim, Doo-Hwan ; Lee, Ju-Hyung ; Shin, Bang-Woo ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 217~222
DOI : 10.7853/kjvs.2013.36.3.217
In this study, we compared nutrient content emphasized on labelling of processed milks with that of whole milks and evaluated how well they conform to labelling standard. A total of 98 samples (33 whole milks, 28 calcium fortified milks, 30 low-fat milks and 7 lactose hydrolyzed milks) were collected in hypermarkets in Seoul from August 28 to August 30 in 2012. Calcium, fat and lactose contents were tested in the samples. Calcium contents ranged 102.2~113.0 mg/100 mL in whole milks and 120.1~337.8 mg/100 mL in calcium fortified milks. The level of calcium contents in fortified milks ranged very broad. Accordingly, the standard of calcium contents on fortified milks will be required. Fat contents ranged 3.1~3.9 g/100 mL in whole milks and 0.1~1.9 g/100 mL in low-fat milks. The average of fats content in low-fat milks was nearly one third than whole milks. Lactose contents was ranged 4.6~5.1 g/100 mL in whole milks and not detected in lactose hydrolyzed milks. All of processed milks were suitable to processing standard and labelling standard. But nutrition claims often used on processed milk such as "High" or "Low" were not adequate to indicate the exact nutrient content, which is a cause of the confusion for milk product labelling to consumers. We need a lot of research about nutrient labelling that can deliver appropriate and understandable information to customers.
Incidence of ascarid eggs in sands of educational facilities and public playground in Seoul
Kwak, Jeong-Yeon ; Seol, Chae-Hyeon ; Seo, Hyeong-Su ; Kim, Gyu-Hyeon ; Kim, Neung-Hee ; Chae, Hee-Sun ; Son, Hong-Rak ; Kang, Yong-Il ;
Korean Journal of Veterinary Service, volume 36, issue 3, 2013, Pages 223~226
DOI : 10.7853/kjvs.2013.36.3.223
To evaluate the potential risk associated with zoonotic ascarid eggs in educational facilities and public playground in Seoul city, the sand samples collected from April to December in 2011 were examined with a fecal flotation method. Of 5,066 sand samples from 1,367 playgrounds tested, 12 sands from 12 places (0.88%) had ascarid eggs. Sand samples in educational facilities were more contaminated (10/870, 1.15%) with the parasite eggs than those of public playground area (2/497, 0.4%). Fisher's exact tests were used to make pairwise comparisons between which locations were more or less similar with regard to levels of contamination. We didn't find significantly different from one another. Most ascarid eggs detected were found in the Spring. Through this study, we hope to raise awareness of contamination of playgrounds by potentially infectious ascarid ova.