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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 37, Issue 4 - Dec 2014
Volume 37, Issue 3 - Sep 2014
Volume 37, Issue 2 - Jun 2014
Volume 37, Issue 1 - Mar 2014
Selecting the target year
Different immunological features of two genetically distinct type 2 porcine reproductive and respiratory syndrome (PRRS) viruses
Shabir, Nadeem ; Khatun, Amina ; Kim, Won-Il ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 1~9
DOI : 10.7853/kjvs.2014.37.1.1
Although it has been generally accepted that porcine reproductive and respiratory syndrome virus (PRRSV) induces weak and delayed protective immunity after infection, it is unclear that the same immunological features can be applicable to all PRRS viruses because huge genetic variation exists even among the same genotypes of PRRSV (Type 1 and 2). In the current study, two genetically distinct type 2 PRRSV strains (VR-2332 and JA142) which showed approximately 90% nucleotide homology based on ORF5 sequences were characterized by both in vitro and in vivo assessments to determine the immunological features of the viruses. For in vitro assessment, porcine alveolar macrophages (PAM) were infected with the viruses at
multiplicity of infection (MOI) and then supernatants and cells were collected separately at 36 hrs post infection to determine the relative expression levels of IL-
, IL-12, TNF-
by quantitative RT-PCR. In addition, five PRRSV-free pigs were inoculated with either of JA142 or VR2332 for in vivo assessment. Serum samples were collected every week until 6 weeks post challenge. The serum samples were analyzed for the levels of viremia, PRRSV nucleocapsid-specific antibody and virus neutralizing antibody. Based on those assessments, the two viruses showed different patterns of cytokine expression in PAM and immune responses in pigs after infection. These results indicate that genetically distinct PRRSV strains have different immunological features, which might be criteria for virus classification and selection of candidate virus strains for vaccine development in the future.
Survey of porcine reproductive and respiratory syndrome (PRRS) on pig farms in Andong and Hapcheon region
Kang, Hye-Won ; Oh, Yooni ; Song, Jae-Young ; Choi, Eun-Jin ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 11~18
DOI : 10.7853/kjvs.2014.37.1.11
Porcine reproductive and respiratory syndrome (PRRS) causes a significant economic loss in the swine industry not only in Korea but also all over the world. Andong and Hapcheon region were selected for Area Regional Control (ARC) programme to reduce the shedding of PRRS virus (PRRSV) and decrease PRRS outbreaks. Before conducting the PRRS ARC, sera of pigs were tested for both antibody using ELISA and antigen using RT-PCR, then phylogenetic classifications was analysed. Pigs of 138/275 (50.2%) in Andong and 352/425 (82.8%) in Hapcheon were seropositive. Also, the RT-PCR results revealed that 27 heads (8.2%) in Andong, 112 heads (22.0%) in Hapcheon were positive for PRRSV antigen. PRRSVs were mainly detected between the ages of 40 to 60 days. PRRSV ORF5 regions were used to determine genetic clusters based on previous report. All PRRSV type I detected in both Andong and Hapcheon were classified as Cluster I. The PRRSV type II isolates in Andong were assorted to Cluster II, whereas the PRRSV type II isolates in Hapcheon were the viruses were unassembled into any cluster except one identified to Cluster III. Phylogenetic analysis indicated that new clusters of PRRSVs type II were prevalent in Hapcheon.
Prevalence of antimicrobial resistance and integrons in extended-spectrum β-lactamases producing Escherichia coli isolated from Nakdong and Gumho river
Cho, Jae-Keun ; Kim, Hwan-Deuk ; Kwon, Soon-Hyo ; Kim, Jin-Hyun ; Jang, Sung-Il ; Park, Choi-Kyu ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 19~27
DOI : 10.7853/kjvs.2014.37.1.19
This study was conducted to investigate the antimicrobial resistance, presence of
-lactamase genes and integrons in 83 ESBL-producing Escherichia coli isolated from Nakdong river and Geumho river in Daegu. Among the
-lactam antimicrobials, all isolates were resistant to ampicillin, cephalothin, cefamandole and cefotaxime, followed by piperacillin (98.8%), ampicillin/sulbactam (86.7%), aztreonam (60.2%) and cefepime (59.0%), whereas resistance to piperacillin/tazobacram, ticarcillin/clavulanic acid and cefoxitin was less than 30%. Many of the ESBL-producing Escherichia coli were also resistant to non-
-lactams antimicrobials such as nalidixic acid (83.1%), sulfonamides (72.3%), ciprofloxacin (62.7%) and gentamicin (38.6%). All isolates showed resistance to seven or more antimicrobial agents. The most frequently detected gene was
(49.4%), followed by
was not found. Class 1 integrons were found in 61.4% (51 isolates) of isolates, however, class 2 and 3 integrons were not detected. The results showed water from Nakdong river and Geumho river is contaminated with ESBL-producing E. coli isolates. These results suggest the need for further investigation of antibiotic resistant bacteria to prevent public health impacts in the water environment.
A highly sensitive molecular diagnosis method for detecting Toxoplasma gondii tachyzoite: a PCR/dot blot hybridization
Hong, Sun-Hwa ; Lee, Yun-Seong ; Kim, Young-Ho ; Kim, Ok-Jin ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 29~33
DOI : 10.7853/kjvs.2014.37.1.29
This study aimed at finding a fast, sensitive, and efficient protocol for molecular identification of intracellular protozoa Toxoplasma (T.) gondii. For molecular detection of T. gondii, we developed a polymerase chain reaction coupled with dot blot hybridization assay (PCR/DBH). For DBH analysis, the amplified DNA of T. gondii tachyzoite was labeled by incorporation of digoxigenin. The DBH assay alone was capable of detecting down to
pg of T. gondii genomic DNA. The PCR alone was capable of detecting down to
pg of T. gondii genomic DNA, whereas the PCR/DBH assay was capable of detecting down to
pg of T. gondii genomic DNA, indicating that sensitivity of the PCR/DBH method was approximately 10 to 100 times higher than PCR or DBH alone. Our PCR/DBH assay will be useful for confirming the presence of T. gondii on the samples and differentiating T. gondii infection from other intracellular protozoa infections.
Protective effect of bacteriophages against Salmonella Typhimurium infection in weaned piglets
Kim, Sung-Jae ; Kim, Jae-Hoon ; Jun, Soo-Yeon ; Paik, Hyoung Rok ; Han, Jeong-Hee ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 35~43
DOI : 10.7853/kjvs.2014.37.1.35
Salmonellosis has caused heavy losses in swine industry and implications for public health. Recently, the urgent problem of antibiotic resistance due to multidrug-resistant Salmonella spp. has been on the rise. The use of host-specific bateriophages as a biocontrol is one possible alternative. In this study, clinical signs, growth performance, quantification and detection of antigen, histopathological changes of gastrointestinal tracts were analyzed comparatively in weaned piglets according to administration of bacteriophages and challenge with Salmonella (S.) Typhimurium. Piglets challenged with S. Typhimurium after administered with bacteriophages showed reduced clinical signs, higher growth performance, lower bacterial shedding, lower quantificational value of antigens in intestines, higher V/C ratio and higher the number of goblet cells in intestines than piglets administered without bacteriophage and challenged with S. Typhimurium. These results indicate that feeding contained with bacteriophages has effect to prevent infection of S. Typhimurium in weaned piglets and suggest that a use of bacteriophage can be considered a valid antibiotic alternative.
Suggestions for a better inspection method according to the occurrence of fowl typhoid in broiler breeders
Chu, Keum-Suk ; Kim, Kyoung-Taek ; Yoon, Eun-Jeong ; Kim, Beom-Seok ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 45~49
DOI : 10.7853/kjvs.2014.37.1.45
Pullorum disease and fowl typhoid are septicemic avian diseases transmitting through egg by transovarian infections. A series of tests has been performing in breeding flocks of chickens and test plans for proper inspection have been modified by government veterinary institute to control of such diseases. To improve inspection plans, different test methods were compared using fowl typhoid positive samples from a poultry farm located in Jeonbuk state in 2012. Based on first inspection, 11 samples among total 200 samples were positive by rapid slide agglutination (RSA) test and 7 samples among RSA positive samples were finally diagnosed as Salmonella Gallinarum infection by ELISA, bacterial isolation, PCR, and histopathologic examination. In the second inspection, 20 samples among total 100 samples were positive by RSA test. Among RSA positive ones, 19 samples were positive by ELISA, S. Gallinarm were successfully isolated in 3 samples, and 16 samples were positive by PCR in the cecal tonsils where were not successful for bacterial isolation. Based on histopathologic examination, severe inflammation in the 13 cecal tonsils and infiltration of lymphocytes and heterophils in the 11 livers were observed. Therefore, we suggest that bacterial isolation, PCR, and histopathologic examination methods in the third inspection need to be further used in various tissues for correct diagnosis and for final eradication of pullorum disease and fowl typhoid in breeding flocks of chickens.
Antioxidant activity and anti-obesity effect of Coprinus comatus in Zucker rat (fa/fa)
Lee, Soo-Jung ; Park, Hyoung Joon ; Song, Yuno ; Jang, Sun-Hee ; Goo, Ae Jin ; Ko, Yeoung-Gyu ; Cho, Jae-Hyeon ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 51~58
DOI : 10.7853/kjvs.2014.37.1.51
The aim of the study was to investigate the anti-obesity effect of Coprinus comatus (CC) in high-fat diet-fed Zucker rat (fa/fa). Obesity was induced by feeding on high-fat diet (HFD) containing 60% kcal fat for 10 weeks, in which CC extracts were administrated through the gastrointestinal tract at a concentration of 200 mg/kg BW/day for 10 weeks. The total polyphenol and flavonoid contents of CC extracts were found to be
of catechin equivalent/g, and
of quercetin equivalent/g extract, respectively. The DPPH, ABTS, and hydroxyl radical scavenging activities of CC extracts were 15.34 %, 17.25%, and 16.18%, respectively. In animal study, CC administration significantly reduced the body weight, while there were no significant differences in the daily food intake between the HFD-fed Zucker rats and HFD plus CC-fed rats. CC treatment decreased epididymal and perirenal fat weights in HFD-fed Zucker rats. Significant decreases in the levels of triglyceride and total cholesterol in the serum and liver were observed in the CC-treated group compared with HFD-fed Zucker rats. Serum HDL-cholesterol levels in the CC-treated group were increased compared with the HFD-fed groups. Serum AST and ALT activities in the CC group were significantly lower than those of the HFD-fed group. Taken together, these data demonstrated that CC has potential in preventing high-fat diet induced obesity and is a good candidate for an anti-obesity agent.
A diagnosis of equine herpesvirus type 1 (EHV-1) myeloencephalopathy using real-time PCR
Choi, Seong-Kyoon ; Kim, Joo-Hyung ; Cho, Gil-Jae ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 59~65
DOI : 10.7853/kjvs.2014.37.1.59
Equine herpesvirus myeloencephalopathy, out of symptoms by equine herpesvirus type 1 (EHV-1) infection, can cause devastating losses on individual farms. Although myeloencephalopathy syndromes of horses in Korea have been recognized for a couple of years in horse populations, there is little study regarding the occurrence of EHV-1 infections. The present study was performed to detect the viral infection of horses with neurological syndrome using real-time PCR. Fifteen horses (27.3%) out of 55 horses with neurological deficiency were positive for EHV-1 viral antigen. Among these 7 horses, 4 horses were detected genotype of A2254/N752 and 3 horses G2254/D752 strain, respectively.
A case of meningitis caused by Streptococcus equi subsp. zooepidemicus infection in a foal in Jeju
Mun, Seong-Hwan ; Yang, Hyoung-Seok ; Jung, Soo-Kyo ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 67~71
DOI : 10.7853/kjvs.2014.37.1.67
In November 2012, a 4-month-old foal with anorexia, neurologic signs with ataxia and death was submitted for necropsy to the diagnostic laboratory of Jeju Self-Governing Provincial Veterinary Research Institute. Grossly, yellowish-white fibrinous materials were seen on the ventral cerebrum, cerebellum, medulla oblongata and cervical spinal cord of the foal. Histologically, severe fibrino-purulent meningitis characterized by the infiltration of neutrophils and fibrionous exudates with bacterial colonies were presented in cerebrum, cerebellum, brain stem and cervical spinal cord. In the bacterial isolation using meningeal exudate, the small white colonies on blood agar plate with
-hemolysis were observed. Isolated bacteria were Gram-positive cocci and confirmed as Streptococcus (S.) equi subsp. zooepidemicus by VITEK 2 system. Based on the histopathological features and the bacterial test, this case was diagnosed as meningitis associated with S. equi subsp. zooepidemicus infection in a foal. In our best knowledge, this is the first report for fibrino-purulent meningitis caused by S. equi subsp. zooepidemicus infection in Jeju.
Encephalitic listeriosis in two Korean native goats in Jeju
Song, Kyoung-Ok ; Yang, Hyoung-Seok ; Jung, Soo-Kyo ; Kang, Wan-Choul ; Ko, Jin-A ; Lee, Chul-Hue ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 73~78
DOI : 10.7853/kjvs.2014.37.1.73
In February 2013, two Korean native goats with circling, dysphagia, sialorrhea, and death were submitted for necropsy to the Jeju Self-Governing Provincial Veterinary Research Institute. Histopathologically, multifocal to confluent micro-abscess composed of neutrophils and macrophages and severe multifocal perivascular cuffing were observed in pons, medulla oblongata, and cerebellum. Isolated bacteria from the brain sample were Gram-positive coccobacilli and were confirmed as Listeria (L.) monocytogenes by VITEK and PCR analysis. Based on clinical sign, histopathology and bacterial isolation, this case was diagnosed as encephalitic listeriosis. In our best knowledge, this is the first report of encephalitic listeriosis caused by L. monocytogenes in Korean native goats in Jeju.
First detection of avian bornavirus by RT-PCR in proventricular dilatation disease-suspected Hahns Macaw (Ara nobilis nobilis) in Korea
Kim, Jin-Hyun ; Lee, Bu-Heung ; Cho, Jae-Keun ; Yoon, Won-Koung ; Kim, Won ; Kim, Hee-Jung ; Kim, Eun-Mi ; Kim, Ki-Seuk ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 37, issue 1, 2014, Pages 79~84
DOI : 10.7853/kjvs.2014.37.1.79
Two Hahns Macaws (Ara nobilis nobilis), three and nine-month-old, with a three-weeks history of weight loss and anorexia were diagnosed presumptively with proventricular dilatation disease (PDD) by radiographic examination. The birds were treated with antimicrobials, analgesics, and fluid administration. However, these birds died three weeks after the first signs of PDD. At necropsy, the birds had severely dilated proventriculus, severe pectoral muscles atrophy, and blood vessels congestion on cortex of cerebrum. The partial matrix gene of avian bornavirus (ABV) was detected by RT-PCR from tissues of the brain, feather calami, and proventiculus of each PDD-suspected birds. This report describes the first detection of ABV in PDD-suspected Hahns Macaw in Korea.