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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
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Volume & Issues
Volume 37, Issue 4 - Dec 2014
Volume 37, Issue 3 - Sep 2014
Volume 37, Issue 2 - Jun 2014
Volume 37, Issue 1 - Mar 2014
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Expression of porcine reproductive and respiratory syndrome virus (PRRSV) ORF7 gene and monoclonal antibody production
Lee, Seung-Chul ; Park, Ga-Hye ; Lee, Kyeong-Won ; Ryu, Min-Sang ; Kang, Shien-Young ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 143~150
DOI : 10.7853/kjvs.2014.37.3.143
Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiological agent of PRRS characterized by reproductive losses in sows and respiratory disorders in piglets. The PRRSV is a small enveloped virus containing a positive-sense, single-stranded RNA genome and divided into two genotype, type 1 (European) and type 2 (North American), respectively, by nucleotide identity. In this study, ORF7 gene of the type 1 and type 2 PRRSV was cloned and expressed in Baculovirus expression system. Also, monoclonal antibodies (MAbs) against ORF7 were produced and characterized. The expressed ORF7 proteins in the recombinant virus were confirmed by indirect fluorescence antibody (IFA) test using His6 and PRRSV-specific antiserum. A total of eight MAbs were produced and characterized. One (3G12) MAb was type 1 PRRSV ORF7-specific and two (6B10 and 16H8) were type 2 PRRSV ORF7-specific. Other five (1A1, 2A4, 4B4, 12C4 and 13F11) MAbs reacted with both type 1 and type 2 PRRSV. Some PRRSV ORF7-specific MAbs recognized the porcine tissues infected with PRRSV by IFA or immunohistochemistry (IHC) assay. From this experiment, it was confirmed that MAbs produced in this study were PRRSV ORF7-specific and could be used as reliable reagents for type 1/type 2 PRRSV detection.
Comparative evaluation of two commercial ELISA kits for detection of PRRS antibodies using sera collected from pigs in various stages of PRRSV infection
Seo, Byoung-Joo ; Kim, Hyoun-Il ; Kim, Won-Il ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 151~156
DOI : 10.7853/kjvs.2014.37.3.151
Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses to the Korean pig industry. ELISA tests using recombinant nucleocapsid protein of PRRSV have been most commonly used for PRRS diagnostics. In the current study, two commercial PRRSV ELISA kits (Bionote PRRSV Antibody ELISA and IDEXX 3XR PRRS Antibody ELISA) have been compared using sera collected from 19 swine farms in various stages of PRRSV infection confirmed by professional diagnostic centers. Thus 130 sera collected from 5 different farms with active PRRSV infection, 130 sera from 6 different farms with PRRS-stabilized status, and 140 sera from 8 different farms with PRRS-free status were evaluated to determine the correlation of test results between those ELISA kits. Both ELISA kits showed a good correlation [PRRSV-positive farms (
Genetic characterization and phylogenetic analysis of Clostridium chauvoei isolated from Hanwoo in Jeonbuk
Kim, Chul-Min ; Jeong, Jae-Myong ; Choi, Ki-Young ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 157~164
DOI : 10.7853/kjvs.2014.37.3.157
Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rated disease infection mainly cattle. In the present study, the partial sequences of 16S rRNA and flagellin gene of C. chauvoei isolated in Jeonbuk, Korea were determined and compared with those of reference strain. Oligonucleotide primers were designed to amplify a 811 bp fragment of 16S rRNA gene and 1229 bp fragment of flagellin gene. Sequencing analysis of 16S rRNA gene showed high homology to the reference strains ranging 82.3% to 100%, while flagellin gene were different from published foreign clostridia, showing 98.7% to 72.0% nucleotide sequence homology. Phylogenetic analysis based on 16S rRNA gene revealed the close phylogenetic relationship of C. chauvoei and C. septicum in cluster I, which includes C. carnis, C. tertium, C. quinii, C. celatum, C. perfringens, C. absonum, C. botulinum B. Phylogentic analysis also revealed that flagellin gene formed a single cluster with C. chauvoei, C. septicum, C. novyi A, C. novyi B, C. tyrobutylicum, C. acetobutylicum. The genetic informations obtained from this study could be useful for the molecular study of C. chauvoei.
Characteristics and antimicrobial resistance patterns of Pasteurella multocida isolated from swine in Gyeongbuk province
Sohn, Jun-Hyung ; Kim, Young-Hoan ; Shin, Seong-Ho ; Lee, Eun-Mi ; Kim, Soon-Tae ; Cho, Min-Hee ; Yun, Mun-Jo ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 165~171
DOI : 10.7853/kjvs.2014.37.3.165
This study was conducted to investigate the species-specific gene detection, capsular serogroup and antimicrobial resistance pattern of Pasteurella multocida isolated from pneumonic lung lesion of swine in Gyeongbuk province. P. multocida isolates were typed for capsular serogroups by polymerase chain reaction. Of the 32 strains, 28 (87.5%) were typed serotype A, 3 (9.3%) were typed serotype D, and 1 strain was unknown (3.1%), respectively. In antimicrobial agents resistance test, almost of strains were susceptible to amoxicillin (100%), enrofloxacin (96.9%), ampicillin (93.8%), florfenicol (90.6%), chloramphenicol (90.6%) and were resistant to streptomycin (71.9%), spectinomycin (56.3%). All strains were resistant to clindamycin, erythromycin and lincomycin.
Survey on the red internal organs gross lesions of slaughtered pigs in Jeonbuk
Chu, Keum-Suk ; Yoon, Eun-Jeong ; You, Ki-Hong ; Ha, Yong-Su ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 173~178
DOI : 10.7853/kjvs.2014.37.3.173
Respiratory diseases are common in swine industry and have great economic importance. Respiratory disorders cause substantial losses to the swine industry. Losses associated with respiratory disease vary considerably between herds and seasons. In a survey was carry out to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data which are useful for each farm. From november 2012 to december 2013, 1,680 pigs out of 84 farms were sampled in Jeonbuk province. Gross lesions such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis, liver milk spot were examined for the pigs. Overall prevalence of SEP was 55.7%. According to season, the incidence occurred higher in fall than winter, spring, and summer. The mean SEP score was 0.91, the highest incidence occurred in fall. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 36.4%, 49.7%, 2.3%, and 8.8%, respectively. The positive rate of PRRS and PCV2 was 2.9% and 70.0% by PCR analysis.
Seroprevalence of abortion and stillbirth inducing disease in Hanwoo, in Jeonbuk eastern area
Song, Ji-Min ; Shon, Ku-Rye ; Koh, Won-Seok ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 179~183
DOI : 10.7853/kjvs.2014.37.3.179
Reproductive losses in a herd can be huge. Fetal reabsorption or undefined infertility often remain undetected. Routine herds monitoring for exposure, controlling the introduction of potential agent carriers, appropriate biosafety procedures, and vaccination where possible are together the best security against abortion and stillbirth inducing disease. For biosecurity of local farms, we performed antibody titers of abortion and stillbirth related diseases such as bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (IBRV), Neospora caninum, Toxoplasma gondii and Campylobacter fetus subsp venerealis. The blood samples were collected from 500 female Hanwoo over 1 year old of 100 farms in Jeonbuk eastern area. Champhylobater serological test was evaluated by the standard tube agglutination test (STAT) and other pathogen`s antibodies were detected by indirect-enzyme linked immunoassay (I-ELISA). The seroprevalence of abortion and stillbirth inducing disease were BVDV 72.4%, IBRV 13.0%, N. caninum 1.2%, T. gondii 10.4% and C. venerealis 0.6%, irrespectively.
Seroprevalence of Metapneumovirus, Reovirus and Mycoplasma in the broiler breeder of Jeonbuk province
Kang, Mi-Seon ; Kim, Seung-Yong ; Lee, Hee-Seon ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 185~190
DOI : 10.7853/kjvs.2014.37.3.185
We investigated the serological prevalence of avian metapneumovirus (AMPV), avian reovirus (ARV), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in 760 broiler breeder (38 flocks), in the Jeonbuk province in 2013. This study was conducted to evaluate the immune and infection status of the broiler breeder flocks against AMPV, ARV, MG, MS by an enzyme-linked immunosorbent assay (ELISA). Serological test for AMPV were positive 37 (97.3%) flocks and 712 (93.6%) broiler breeder and geometric mean antibody titers were
, ARV were high positive rate 100% (38/38) flocks and 97.8% (743/760). The seropositive flocks against MG were 71.1% (27/38) and the geometric mean antibody titers were
, whereas the rates of positive flocks against MS were 50.0% (19/38) and the geometric mean antibody titers were
The survey on contents of preservative and general composition in cheese
Han, Hye-Jin ; Kim, Yoen-Joo ; Lee, Kyung-Hye ; Yun, Min ; Kim, Young-Seob ; Lee, Ju-Hyoung ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 191~196
DOI : 10.7853/kjvs.2014.37.3.191
This study was conducted to compare the contents of preservatives and general composition on four different types of cheese to provide useful data and information to the consumer. We analyzed preservatives (sorbic acid, benzoic acid, dehydroacetic acid, methyl p-hydroxy benzoate, ethyl p-hydroxy benzoate, propionic acid) and general composition (moisture, protein, fat) from total 102 cheeses which are 46 sliced cheeses, 22 string cheeses, 20 cream cheeses and 14 pizza cheeses. Preservatives were detected from total 14 samples, which are suitable for the authorized limits in Korea. Sorbic acid was detected from 13 samples (197.3~1,736.1 mg/kg) and propionic acid was detected from 1 sample (362.7 mg/kg). Considering from each type of cheese, cream cheese showed the high detection rate (60%); 12 samples of cream cheese contained sorbic acid. The moisture, protein and fat contents of cheeses were in the ranges of 44.3~56.8%, 7.2~24.6% and 19.6~26.8%. Cream cheese had the highest level of moisture (56.8%) and fat (26.8%), but protein level is very low (7.2%).
Investigation of Dirofilaria immitis infection in stray dogs from public animal shelters in Seoul
Kim, Neung-Hee ; Kwak, Jeong-Yeon ; Kim, Hye-Ra ; Park, Hyeong-Suk ; Kim, Doo-Hwan ; Lee, Ju-Hyung ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 197~202
DOI : 10.7853/kjvs.2014.37.3.197
Dirofilaria immitis infection is one of the most important parasitic diseases in dogs and public health. It often elicits nodules in the pulmonary parenchyma and in the subcutaneous tissues of human. In this study, we investigated the prevalence of D. immitis infection among 754 dogs in Seoul area from April to December 2012. The infection rate of 754 dogs was 9.8% (74/754) by the antigen test (canine SNAP 4Dx). The infection rates of D. immitis in dogs at the age of < 2, 2~4, 5~7, 8~10, and > 10 years were 3.7% (7/189), 12.9% (30/233), 15.0% (19/127), 9.8% (18/184), and 0% (0/21), respectively. The infection rates in the female and male dogs were 9.7% (33/340) and 9.9% (41/414), respectively. The regional infection rates in northern and southern Han river region of Seoul were 10.7% (44/410) and 8.7% (30/344), respectively. In order to confirm D. immitis infection, we performed PCR on serum samples of 74 dogs which tested positive for D. immitis antigen by the antigen test and we detected a specific gene ITS-2 in 59 serum samples. In conclusion, this study suggests that treatment and preventive care on the D. immitis infection should be considered in dogs of Seoul area.
Case Report: Mass death of frogs (Rana dybowskii) caused by septicemia in artificial raising farm
Jeong, Yeo-Jin ; Kim, Jong-Taek ; Suh, Guk-Hyun ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 203~212
DOI : 10.7853/kjvs.2014.37.3.203
Frog culture industry is not yet familiar but has much potential. Generally, in farm, the population density is higher than that of in nature and frog farm is not the exception. But when population density is high, it can easily leads to stressful condition, poor sanitation. When a disease occur, it is a primary factor that makes the population more susceptible and the results more grave. Because of severe Rhabditoidea- helminth infection and subsequent bacterial septicemia, 50~70% of the total population had been died in a farm in Jeong-sun in Gangwon-do and Chungju in Chungcheongbuk-do from late June, 2012 to September, 2012. Diseased frogs showed ruptured lung, bloody ascites, liver discoloration, myocardium weakness, congested kidney, microcytic anemia and so on. Enterobacteriacea, Citrobacter.sp, Cupriavidus metallidurans, Acinetobacter.sp were isolated as major bacterium that had caused septicemia in frogs. Among isolated bacterium, Cupriavidus metallidurans, Ewingella americana, Shewanella aquimarina and Pseudoalteromonas sp. have not reported as potential pathogens in frogs before. It is a good example that severe helminth infection in frogs can lead to secondary infection of bacteria.
First detection of avian polyomavirus by PCR from Alexandrine Parakeet (Psittacula eupatria) in Korea
Kim, Hee-Jung ; Lee, Sun-Rock ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 213~218
DOI : 10.7853/kjvs.2014.37.3.213
In early April 2014, a month-old Alexandrine Paraqeet (Psittacula eupatria) that was raised in a domestic aviary located in Gyungju-si, Korea was suddenly died and submitted to Animal Disease Intervention Center, Kyungpook National University in order to diagnose the causative agent. In post-mortem examination, the bird had abnormally developed feathers on the neck and abdomen region and subcutaneous hemorrhages on the neck and cheek adjacent to the beak. At necropsy, the bird had hemorrhage on the muscle of the femoral region, ascites, multi-focal hemorrhages on the epicardium, and diffuse hemorrhages on the sub-serosa of proventriculus and gizzard, suggesting typical avian polyomavirus (APV) infection. The partial large tumor (T) antigen gene of APV was detected by PCR from tissues of the heart, lung, liver, kidney, proventriculus and feathers of the APV-suspected birds. However, other pathogenic virus-specific nucleic acid common with psittacine birds such as avian bornavirus, psittacine beak and feather disease virus and psittacid herpesvirus were not detected from the mixed tissue samples of the bird, indicating this case is due to single infection of APV. Nucleotide sequence analysis of the partially amplified large T antigen DNA was confirmed to have 99~100% homology with that of the previously reported APV strains. This case report describes the first detection of APV in Alexandrine Paraqeet in Korea.
Detection of psittacine beak and feather disease virus from a caged blue and yellow macaw (Ara ararauna) in Korea
Kim, Hee-Jung ; Kang, Dae-Young ; Kim, Eun-Mi ; Kim, Eun-Gik ; Lee, Bu-Heung ; Yeo, Sang-Geon ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 37, issue 3, 2014, Pages 219~224
DOI : 10.7853/kjvs.2014.37.3.219
A eight-month-old blue and yellow macaw (Ara ararauna) with psittacine beak and feather disease (PBFD)-suspected signs, such as, abnormal feather, depression and diarrhea, was presented to Animal Disease Intervention Center, Kyungpook National University in 16 April 2014. The partial ORF V1 gene of PBFD virus (PBFDV) was detected by polymerase chain reaction (PCR) from DNA templates extracted from feather, blood and cloacal swab sample of the bird, but no other viral DNAs that often infected in psittacine birds including avian bornavirus and avian polyomavirus were detected from the samples of the bird, indicating this case is due to single infection of PBFDV. Nucleotide sequence analysis of the amplified partial ORF V1 gene was confirmed to have 96.7% and 93.6% homology with that of previously reported PBFDV strain (Genbank no. HM748924 and FJ685980). This report describes the first detection of PBFDV in PBFD-suspected blue and yellow macaw in Korea.