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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 37, Issue 4 - Dec 2014
Volume 37, Issue 3 - Sep 2014
Volume 37, Issue 2 - Jun 2014
Volume 37, Issue 1 - Mar 2014
Selecting the target year
Characterization of cefotaxime-resistant Escherichia coli isolated from wastewater treatment plant in Daegu
Kim, Hwan-Deuk ; Park, Dae-Hyun ; Lee, Mi-Ree ; Kim, Eun-Jeong ; Cho, Jae-Keun ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 225~231
DOI : 10.7853/kjvs.2014.37.4.225
In this study, 185 cefotaxime-resistant Escherichia coli were isolated from different stages of a wastewater treatment plant (WWTP) in Daegu in Korea. Among them, 99.5% (184 isolates) originated from raw sewage and 0.5% (1 isolates) from the final effluent. Cefotaxime-resistant E. coli were high resistant to ampicillin, piperacillin, cefazolin, cephalothin, cefachlor and cefamandole (99.5~100%). About 93% of the cefotaxime-resistant E. coli were extended-spectrum
-lactamases (ESBL)-producing E. coli. The
gene was the most predominant of the ESBL genes (72.5%), followed by
(respectvely 0.5%). Class 1 and 2 integron were found in 49.7% and class 3 integron was not found. All of integron positive isolates were multiresistant (i.e. resistant to four or more antibiotics). Our findings showed WWTP is contaminated with antibiotic resistant bacteria with resistance genes.
Correlation between the Salmonella seroprevalence on farms and the isolation rate from slaughtered pigs and antimicrobial resistance from the isolates
Kim, Kyung-Eon ; An, Jong-Min ; Yang, Byung-Hun ; Park, Yeong-Hee ; Park, Mi-Young ; Jung, Joon-Yong ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 233~240
DOI : 10.7853/kjvs.2014.37.4.233
Salmonella spp. are one of the most common bacteria that causes heavy losses in swine industry and have implications for public health. In this study, the correlation between Salmonella seroprev-alence on farms and the isolation rate from slaughtered pigs was analyzed and the antimicrobial resistance of the isolated Salmonella spp. was investigated. A total of 3,001 serum samples for ELISA were collected from 17 farms during two consecutive years (2012-2013). The mean values of ELISA OD% for each 8 age groups were as follows; gilt 27.83 (n=472), sow 23.75 (n=367), 150 days (d) of pig 16.53 (n=278), 130 d 11.87 (n=366), 100 d 9.46 (n=378), 20 d 9.17 (n=394), 70 d 6.56 (n=382), 40 d 3.72 (n=364). From July 2013 to January 2014, a total of 53 (8.0%) Salmonella strains were isolated from 665 slaughtered pigs shipped from those 17 farms. The mean values of ELISA OD% for each age groups serum samples that were collected in the second half of 2013 showed a positive correlation at 100 d (0.61, P<0.05), 130 d (0.45, P<0.1) with the isolation rate of Salmonella spp. in the slaughtered pigs. All the isolates were identified by a real-time PCR and tested for antimicrobial susceptibility. As a result, the predominant serovar was S. Typhimurium (52.8%) and there were 15 strains showing their own antimicrobial resistance pattern. All strains were susceptible to amoxicillin, cefepime, ciprofloxacin and some of them were resistant to streptomycin and tetracycline (60%), ampicillin (53.3%), chloramphenicol (33.3%), respectively.
Seroprevalence of Coxiella burnetii in native Korean goats (Capra hircus coreanae) in Gyeongbuk province, Korea
Kim, Seong-Guk ; Cho, Jae-Cheong ; Lee, Min-Gyo ; Kim, Seon-Soo ; Lee, Seung-Hun ; Kwak, Dong-Mi ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 241~246
DOI : 10.7853/kjvs.2014.37.4.241
Q fever is a zoonotic disease caused by the obligate intracellular bacterium Coxiella (C.) burnetii and affects wild and domestic animals worldwide. The aim of this study was to evaluate the seroprevalence of C. burnetii in native Korean goat (Capra hircus coreanae) in Gyeongbuk province, Korea, using ELISA. A total of 256 goat blood samples from 56 farms in Gyeongbuk province were collected between May 2012 and March 2013. Among them, 22 (8.6%) samples from 10 (17.9%) farms were seropositive for C. burnetii by ELISA. According to regional analysis, the seroprevalences among goat farms in eastern, western, southern, and northern areas of Gyeongbuk province were 0%, 18.2%, 36.8%, and 6.3%, respectively, showing the highest seroprevalence in the southern region. Among 22 counties in Gyeongbuk province, 10 (45.5%) counties had one or more farms positive to C. burnetii antibody. Accordingly, the seroprevalence of C. burnetii in high-risk humans and animals are constantly demanded by regional investigation.
Simultaneous diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections by multiplex PCR
Hong, Sunhwa ; Lee, Hyun-A ; Kim, Dong-Woo ; Kim, Tae-Wan ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 247~252
DOI : 10.7853/kjvs.2014.37.4.247
The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.
UNG-based direct polymerase chain reaction (udPCR) for the detection of porcine circovirus 2 (PCV2)
Kim, Eun-Mi ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 253~261
DOI : 10.7853/kjvs.2014.37.4.253
Porcine circovirus disease (PCVD) is a major problem of swine industry worldwide, and diagnosis of PCV2, causal agent of PCVD, has been doing in clinical laboratories of pig disease by polymerase chain reaction (PCR) methods. But the PCR analyses have a serious problem of misdiagnosis by contamination of DNA, in particular, from carryover contamination with previously amplified DNA or extracted DNA from field samples. In this study, an uracil DNA glycosylase (UNG)-based direct PCR (udPCR) without DNA extraction process and DNA carryover contamination was developed and evaluated on PCV2 culture and field pig samples. The sensitivity of the udPCR combined with dPCR and uPCR was same or better than that of the commercial PCR (cPCR) kit (Median diagnostics, Korea) on PCV2-positive serum, lymph node and lung samples of the pigs. In addition, the udPCR method confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PCV2 DNA from previous udPCR. In clinical application, 170 pig samples (86 tissues and 84 serum) were analysed by cPCR kit and resulted in 37% (63/170) of positive reaction, while the udPCR was able to detect the PCV2 DNA in 45.3% (77/170) with higher sensitivity than cPCR. In conclusion, the udPCR developed in the study is a time, labor and cost saving method for the detection of PCV2 and providing a preventing effect for DNA carryover contamination that can occurred in PCR process. Therefore, the udPCR assay could be an useful alternative method for the diagnosis of PCV2 in the swine disease diagnostic laboratories.
One step multiplex RT-PCR preventing DNA carryover contamination for differential diagnosis of swine influenza viruses
Kim, Hee-Jung ; Kim, Eun-Mi ; Shin, Yeun-Kyung ; Song, Jae-Young ; Kim, Seong-Hee ; Lee, Kyoung-Ki ; Lee, Myoung-Heon ; Kim, Young-Hwa ; Park, Jun-Cheol ; Yeo, Sang-Geon ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 263~271
DOI : 10.7853/kjvs.2014.37.4.263
In this study, we developed a cost and time saving one-step multiplex RT-PCR for the simultaneous detection and differentiation of swine influenza viruses (SIV) and 2009 pandemic influenza H1N1 virus (pH1N1). The one-step multiplex RT-PCR using four sets of primer was confirmed to be capable of detection of all SIV subtypes and differential diagnosis of major SIV subtype H1, H3 and pH1N1 on individual or mixed viral culture samples. The sensitivity of the multiplex RT-PCR was determined to be at least
of the presented SIVs, providing sufficient efficacy for a routine SIV monitoring in diagnostic laboratories. In addition, compared with the conventional RT-PCR methods that cannot avoid the carryover DNA contamination, the developed RT-PCR applied with the uracil DNA glycosylase (UNG) system was proven to prevent a false positive reaction by carryover contamination of the pre-amplified DNA. In conclusion, the one-step RT-PCR with UNG system could be applicable to detect and differentiate of SIV from the viral cultures without worry of carryover DNA contamination in clinical laboratories.
Investigation of hematological analyses and major viral serum antibody titers in DongGyeongi
Choi, Seog-Gyu ; Sung, Gi-Chang ; Lee, Eun-Woo ; Park, Chang-Eun ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 273~280
DOI : 10.7853/kjvs.2014.37.4.273
Investigations of hematologic and canine distemper virus, parvovirus antibody titer for DongGyeongi were performed. This study was conducted to determine into feeding and management blood values on DongGyeongi. Blood samples were collected from 110 healthy dogs (male 60, female 50). The diagnostic virus disease and hematologic results were classified by age, sex, color. Although gender differences were not apparent, complete blood cell counts analyses were performed and analyzed accordingly. Statistically significant differences (P<0.05) specific to age (<2). Also, The canine distemper virus and parvovirus antibody titer were correlation to age dependent (P<0.05). In conclusion, data obtained from this study may be valuable as a standard for interpretation of the results in hematologic and major viral antibody titer analysis of DongGyeongi populations. For the management of DongGyeongi, the programs will be used for the epidemic disease prevention.
The health status of stray dogs and cats adopted to public animal shelter in Gwangju area, Korea
Na, Ho-Myung ; Choi, Jong-Woog ; Park, Jae-Sung ; Lee, Yeun-Ey ; Bae, Seong-Yeol ; Park, Seong-Do ; Kim, Eun-Sun ; Kim, Yong-Hwan ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 281~290
DOI : 10.7853/kjvs.2014.37.4.281
The purpose of this study was to determine the zoonotic diseases of stray dog and cat in Gwangju, Korea. We chose randomly 300 samples which is 265, dogs and 35, cats in the public animal shelter from March to August of 2013. The animals' blood biochemistry values measured, and the out of normal values were 49.7% GOT, 36.3% GPT, and 78.0% GGT. The tested items were Dirofilaria immitis, Toxoplasma gondii, Brucella canis, Rabies virus. The positive results were 10% Dirofilaria immitis, 6.3% Toxoplasma gondii (antibody detected), 10% Rabies (antibody detected) but 0.0% in B. canis. The stray animals' antibody antigen positivity take effect high from Mar.-May. Therefore, we propose that those diseases should be monitering and vaccinating in Korea.
Simplification of the HACCP prerequisite requirements evaluation items for butcher shops
Hong, Chong-Hae ; Yoon, Jang-Won ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 291~296
DOI : 10.7853/kjvs.2014.37.4.291
HACCP system application has been expanded to livestock products chain from farm to table in Korea. However, it requires flexible application with modified simple model in order to activate its application in small businesses such as butcher shop. We calculated non-compliance rates to identify weak points of prerequisite requirements and choosed main contents to be controlled as a simplified prerequisite program in butcher shops. The data used for the analysis of non-compliance rates were based on the HACCP accreditation evaluation inspection reports conducted by Korea Livestock Products HACCP Accreditation Service. Among the existing 51 evaluation items, we suggested 28 items of prerequisite requirements for the butcher shops.
Animals and diseases prevalence of the rescued dogs in an animal shelter in Incheon
Yun, Ga-Ri ; Jung, Eun-Ha ; Ra, Do-Kyung ; Jeong, Cheol ; Lee, Kyung-Min ; Chae, Hyun-Woo ; Lee, Jung-Goo ; Lee, Sung-Mo ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 297~305
DOI : 10.7853/kjvs.2014.37.4.297
Animals and disease frequency of the rescued dogs were investigated in Incheon Veterinary Medical Association Animal Shelter from January in 2012 to December in 2013. Three zoonoses (rabies, brucellosis, and dirofilariosis) and three infectious diseases (canine distemper, canine parvoviral enteritis, and canine influenza) were examined for stray dogs. Among 5,603 heads, 647 (11.5%) went back to their owner and 969 (17.3%) were adopted to new families. Prevalence of dirofilariosis, canine distemper and canine parvoviral enteritis were 2.2% (16/718), 6.0% (24/399) and 6.1% (24/396), respectively. Positive antibody rates against rabies, B. canis and canine influenza virus were 20.5% (41/200), 0.1% (1/718) and 2.0% (4/200), respectively. Protective antibody for canine distemper virus and canine parvovirus were shown in 47.0% (94/200). The data indicate that control measures including facility standards and disease control program are one of the important aspects of the shelter management because stray dogs are exposed to various infectious agents.
Survey of fecal coliform contamination in sands of educational facilities and parks in Seoul
Kim, Hye-Ra ; Kim, Neung-Hee ; Park, Hyong-Suk ; Kim, Doo-Hwan ; Lee, Ju-Hyung ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 307~312
DOI : 10.7853/kjvs.2014.37.4.307
Fecal coliform bacteria are indicators of fecal contamination and of the potential presence of pathogens associated with animal excreta, wastewater, sludge. This study examined the distribution of fecal coliform in sand of the playground at educational facilities and parks in Seoul. Fecal coliform were detected from 61 (22.1%) places out of 276 playgrounds. Escherichia (E) coli (75.4%) was found most frequently, followed by Cronobacter (Cro) sakazakii (8.7%), Klebsiella (K) pneumoniae (7.2%) and Actinobacter baumannii (4.3%). We also tested the bacteria for their susceptibility to 16 antibiotics using disk diffusion method. All the isolates were highly susceptible to cefepime, gentamicin, tetracycline, ciprofloxacin, enrofloxacin, nalidixic acid and sulfamethoxazole/ trimethoprim, but resistant to apramycin and cephalothin. E. coli and Cro. sakazakii were highly resistant to apramycin (100% and 83.3%), followed by cephalothin (51.9% and 50%) and ampicillin (19.2% and 16.7%). K. pneumoniae was highly resistant to ampicillin and apramycin (100%, respectively) but the remaining antibiotics were susceptible.
An occurrence of mammary spindle cell carcinoma in a dog
Hong, Sunhwa ; Lee, Hyun-A ; Kim, Dong-Woo ; Kim, Tae-Wan ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 37, issue 4, 2014, Pages 313~317
DOI : 10.7853/kjvs.2014.37.4.313
A bitch was presented for investigation of the mass in left 5th mammary gland. The partial mastectomy was performed and submitted for the histopathological diagnosis. The mammary mass was firm and white colored. The cut surface was separated with several lobules and developed vessels. The central area of the mass formed the cavity filled with inflammatory exudates. The dominant component of the tumor was the bundles of spindle-shaped cells. Some tumor cells possessed atypical nuclei and were arranged in a solid nest. Cysts were microscopically composed of hemorrhage, necrosis, and exudates, partially surrounded by tumor cells and granulation tissues. Histopathologically, the mammary mass revealed spindle cell carcinoma. The bitch made a complete recovery following the mastectomy. This case was a rare mammary spindle cell carcinoma in a dog.