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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Volume & Issues
Volume 38, Issue 4 - Dec 2015
Volume 38, Issue 3 - Sep 2015
Volume 38, Issue 2 - Jun 2015
Volume 38, Issue 1 - Mar 2015
Selecting the target year
Investigation of atrophic piglets diseases in northern area of the Gyeongnam province, Korea
Kim, Hyeong-Su ; Seong, Min-Ho ; Han, Kwon-Seek ; Park, Jung-Yong ; Shin, Yoo-Gyeong ; Jeong, Myeong-Ho ; Park, Dong-Yeop ; Koh, Phil-Ok ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 1~7
DOI : 10.7853/kjvs.2015.38.1.1
This study was investigated to diagnose pathogenic organisms of atrophic piglets in northern area of the Gyeongnam province, Korea. Samples such as feces, blood and necropsy specimens of 42 atrophic piglets (
weeks old) were taken from May to December 2013 for this survey. Samples were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) assay and bacteria isolation for detection of pathogenic agents. 93 pathogens were isolated from 42 samples can be classified into ll groups. We identified bacterial agents in 56 cases (60.2%) and viral agents in 31 cases (33.3%). However, 6 cases (6.5%) were undetected. Among these pathogens, the most prevalent disease were porcine reproductive and respiratory syndrome (PRRS) in 22 cases (23.7%). The major diseases were Colibacillosis in 15 cases (16.1%), Glasser`s disease in 12 cases (12.9%), and porcine epidemic disease (PED) in 9 cases (9.7%). Mixed infections were accounted for 77.8% of atrophic piglets. In particular, the rate of mixed infections with PRRS virus showed the highest frequency (71.4%). In addition, there is a seasonal variation. Viral pathogens were dominantly detected in winter, but in the rest of the season bacterial agents were mainly detected. Gastrointestinal diseases occurred mainly in the pre-weaning piglets, the respiratory diseases and wasting diseases occurred mainly in the post-weaning piglets.
Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) in Gyeongbuk province
Sohn, Jun-Hyung ; Shin, Sung-Ho ; Lee, Eun-Mi ; Kim, Soon-Tae ; Cho, Min-Hee ; Yun, Mun-Jo ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 9~12
DOI : 10.7853/kjvs.2015.38.1.9
The purpose of this study was survey of porcine reproductive and respiratory syndrome (PRRS) virus antibody in Gyeongbuk province by enzyme-linked immunosorbent assay (ELISA). Total 690 samples collected from 15 pig farms were tested. The overall seroprevalence of PRRS virus antibodies was 63.2% (436/690) and 13 farms of 15 farms had at least one pig with PRRS virus antibodies. The seroprevalence of PRRS virus antibody varied with age. Results in 1 to 30 day old, 31 to 60 day old, 61 to 90 day old, 90 to 120 day old and over 120 day old pig were 58.3%, 36.0%, 68.0%, 84.0%, 80.0% and sow were 61.9% respectively.
Survey of foot-and-mouth disease virus structural protein antibody titer in Yeongcheon
Sohn, Jun-Hyung ; Hwang, You-Sun ; Sohn, Kyu-Hee ; Shin, Sung-Ho ; Lee, Eun-Mi ; Kim, Soon-Tae ; Cho, Min-Hee ; Yun, Mun-Jo ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 13~17
DOI : 10.7853/kjvs.2015.38.1.13
Three serotypes (O, A and Asia1) of the foot-and-mouth disease (FMD) vaccine were injected into domestic cloven-hoofed animals in korea after the nationwide spread at the end of 2010. The purpose of this study was survey of FMD virus stuructural protein (SP) antibody titer in Yeongcheon by enzyme-linked immunosorbent assay (ELISA). Total 1,324 samples collected from 89 farms were tested. The overall seroprevalence of FMD virus SP antibodies was 58.8% (778/1,324) The seroprevalence of FMD virus SP antibody varied with species. Results in cattle (over 12 month old) and pig (90 to 130 day old) were 58.8% and 44.9% respectively.
Distribution and antimicrobial susceptibility patterns of bacteria isolated from genital tract of riding mares
Cho, Young-Jae ; Lee, Yong-Duck ; Jang, Jong-Duck ; Shin, Kwang-Hyeu ; Park, Yong-Soo ; Yang, Jae-Hyuk ; Kim, Sung-Joon ; Cho, Gil-Jae ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 19~23
DOI : 10.7853/kjvs.2015.38.1.19
This study was carried out to investigate the genital tract bacterial flora of riding mare in Jangsu stud farm during March to September, 2014. The specimens were collected from vaginal and uterus using a swab from 104 riding mares. Colonies were selected on blood and MacConkey agar plates, and identified as standard biochemical properties and Maldi-Tof MS. From this study, we isolated 148 strains including Escherichia (E.) coli (14.19%), Streptococcus (S.) equi subsp. zooepidemicus (2.7%), Streptococcus (S.) dysgalactiae subsp. equisimilis (2.03%), Klebsiella (K.) pneumonia (1.35%) and other strains from riding mares. In antimicrobial agents susceptibility test, it showed a high sensibility to the antibiotics of the most. E. coli and S. zooepidemicus were visible to have a high sensibility to almost antibiotics used in this study. However, K. pnemoniae showed a high antibiotic resistance patterns. These results may provide the basic information to establish strategies for the treatment and prevention of reproductive diseases in riding mares in Korea.
Analysis of bee venom residues in milks of dairy cattle using UHPLC with newly developed pre-processing method
Han, Sang-Mi ; Hong, In-Pyo ; Woo, Soon-Ok ; Kim, Se-Gun ; Jang, Hye-Ri ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 25~30
DOI : 10.7853/kjvs.2015.38.1.25
Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.
Detection of Mycoplasma felis from the kenneled cats with pneumonia
Hong, Sunhwa ; Lee, Hak-Yong ; Kim, Tae-Wan ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 31~36
DOI : 10.7853/kjvs.2015.38.1.31
Two cats were obtained from a cat kennel. Over the previous 7 days, the cats had shown cough, anorexia, depression and nasal discharge. In this study, the consensus PCR was able to detect successfully Mycoplasma species in nasal swab samples of the cats. To identify feline mycoplasma species from the lung tissue of the cats with pneumonia, Mycoplasma species-specific PCR reactions were conducted. As the results, we could identify M. felis by the positive amplified DNAs. On the other hand, we could not detect any positive reactions with the PCR reaction for M. arginini, M. canis, M. edwardii, M. cynos, M. gateae, M. maculosum, M. molared, M. opalescens, M. spumans and Mycoplasma HRC-689. In conclusion, we detected M. felis from the kenneled cats with pneumonia. We suggested that this consensus PCR would be useful and effective for monitoring Mycoplasma species in various kinds of animals including cats. The application of preceding consensus PCR before the species-specific PCRs may be the most recommended strategy for the identification of Mycoplasma spp.
Comparison of the non-invasive diagnostic methods, stool antigen test and PCR assay, for Helicobacter felis detection in dogs
Hong, Sunhwa ; Lee, Hak-Yong ; Kim, Tae-Wan ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 37~42
DOI : 10.7853/kjvs.2015.38.1.37
The aim of the present study was to compare the non-invasive methods for the diagnosis of H. felis with HpSA kit-based detection method and H. felis-specific PCR assay with dog`s stool samples without sacrifice. Male Beagle dogs (n
Study on prevalence of antigens to bovine viral diarrhea virus (BVDV) of Cattle in Busan area (2013~2014)
Kim, Hong-Tae ; Park, Min-Sik ; Lee, Gi-Heun ; Lee, Keun-Woo ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 43~49
DOI : 10.7853/kjvs.2015.38.1.43
Bovine viral diarrhea virus (BVDV) is a very important viral disease virus in cattle, domestic and wild ruminants. The purpose of this study is to investigate the positive rate of bovine viral diarrhea virus antigen by ELISA from Korean native and beef cattle reared in Busan area from March in 2013 to October in 2014. A total of 1,129 bovine blood samples were collected from 140 farms, 1,111 Korean native cattle of 135 farms and 18 beef cattle of 5 farms. Test for antigen was carried out by ELISA method. In general analysis, the positive rate of bovine viral diarrhea virus antigen were 0.7% (8/1,129) cattle and 5.0% (7/140) farm. In regional analysis, the positive rate of BVDV antigen of farm in Kijang-gun, Gangseo-gu, Geumjeong-gu, Saha-gu and Dongnae-gu were 1.4% (2/94), 3.6% (5/37), 0% (0/7), 0% (0/1) and 0% (0/1), respectively, and the positive rate of BVDV antigen of cattle were 0.4% (3/770), 1.5% (5/333), 0% (0/24), 0% (0/1) and 0% (0/1), respectively. The positive rate of BVDV antigen according to sex were 0.6% (6/1,085) female cattle and 4.6% (2/44) male cattle. According to the age of cattle, the positive rate of BVDV antigen in 1 year, 2 years, 3 years and 5 years old were 1.9% (4/215), 0.4% (1/265), 0.9% (2/234) and 1.0% (1/103), respectively, but 4 years (0/198), 6 years (0/55), 7 years (0/24), 8 years (0/14), 9 years (0/10), 10 years (0/7) and 11-15 years (0/3) old were negative, respectively.
Genetic characterization and phylogenetic analysis of infectious bronchitis virus isolated in Jeonbuk
Chu, Keum-Suk ; Kim, Ji-Hyun ; Lee, Jeong-Won ; Choi, Kwang-Lim ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 51~56
DOI : 10.7853/kjvs.2015.38.1.51
Infectious bronchitis virus (IBV) causes an acute and highly contagious viral disease of chicken that is great economic losses to the poultry industry worldwide. Among the IBV structural proteins, the high rate spike glycoprotein S1 gene mutation and antigenic variant strains have been reported in many countries. During the years 2012~2014, 10 IBV strains were isolated from infected chicken farms distributed in provinces of Jeonbuk. Analysis of the S1 gene sequences amplified from 10 isolated strains with QX strains showed nucleotide homologies ranging from 96.5 to 95.4%. Phylogenetic analysis revealed that all strains were clustered into QX-like groups. This study suggests that QX-like IBVs are circulating in commercial chicken farms in Jeonbuk. Therefore, the continuing survellance is significantly important for prevention and control of BIV infection.
Monitoring of Sacbrood virus from Korean native honeybees in Jeonbuk province, Korea
Shon, Ku-Rye ; Kim, Ji-Hyun ; Chu, Keum-Suk ; Lee, Jeong-Won ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 57~59
DOI : 10.7853/kjvs.2015.38.1.57
This study was carried out to investigate the Sacbrood virus (SBV) of Korean native honeybees causing serious damage in Jeonbuk area. Korean native honeybees completing the after overwinter 60 farms and March to April active phase adult bees and larvae 52 farms were collected from farms in 7 counties. Active phase of the adult bees 39 (75.0%) and larvae 24 (46.2%) farms was infected with SBV in 52 farms. This result indicate that SBV was the highest in Imsil-gun than other areas.
Clinical characteristics of highly pathogenic avian influenza virus (H5N8) in Jeonbuk province of Korea, 2014
Jeong, Jae-Myong ; Kim, Chul-Min ;
Korean Journal of Veterinary Service, volume 38, issue 1, 2015, Pages 61~64
DOI : 10.7853/kjvs.2015.38.1.61
Highly pathogenic avian influenza (HPAI) occurred in the breeder duck farms in Jeonbuk of in Korea on January to February 2014. Clinically, the most ducks showed various signs from depression, dropped egg production and feed consumption to even, death. The most commonly gross changes were hepatomegaly, splenomegaly, petechial and ecchymotic hemorrhage on the liver surface, a white stripe on the cardiac muscle, multifocal hemorrhagic foci in pancreas, and severely hemorrhagic embryos. The most significant signs of H5N8 virus was supposed to specific on ducks. The viral antigen was mainly detected in the endothelium of blood vessels of various organs and tissues, peripheral nerves, and neuronal cells. Based on the above results, we identified that HPAI H5N8 induced systemic infection in the adult breeder ducks.