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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 38, Issue 4 - Dec 2015
Volume 38, Issue 3 - Sep 2015
Volume 38, Issue 2 - Jun 2015
Volume 38, Issue 1 - Mar 2015
Selecting the target year
In vitro antimicrobial activity of Korean propolis against fish pathogenic bacteria
Heo, Gang-Joon ; Won, Tae-Gyeong ; Shin, Gee-Wook ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 65~70
DOI : 10.7853/kjvs.2015.38.2.65
The present study was to investigate in vitro antimicrobial effects of propolis against six different fish bacterial pathogens, Aeromonas hydrophila, Edwardsiella tarda, Vibrio vulnificus, V. parahaemolyticus, A. salmonicida subsp. masoucida, A. salmonicida subsp. salmonicida) using minimum inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) tests. In the results, propolis exhibited antimicrobial activity against all bacteria used in the present study, but there was no marked difference in bacterial species except Vibrio species. Collectively, propolis was thought to be an usefulness antimicrobial substance for controlling bacterial diseases in the fish industry.
Seroprevalence of Salmonella Typhimurium in the pigs raised in Jeju Province, Korea
Yang, Hyoung-Seok ; Kang, Wan-Choul ; Ko, Jin-A ; Bae, Jong-Hee ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 71~75
DOI : 10.7853/kjvs.2015.38.2.71
Porcine salmonellosis is an economically important disease affecting the global pig industry today. Salmonella (S.) Typhimurium is highly contagious and may rapidly spread within pig populations of herd. To investigate the prevalence of porcine salmonellosis in Jeju, a total of 12,885 blood sera of 96 pig farms from 2009 to 2012 were analyzed by microplate agglutination test. Antibodies to S. Typhimurium were detected in all of pig farms tested in Jeju Province, and the mean of seropositive rate of individual pig was 18.8%. The mean seropositive rate of S. Typhimurium in sows (46.7%) was 7 times higher than that of weaned or growing pigs (6.7%). The lowest seropositive rate (3.0%) was detected in 40 day-old pigs, and this result might be closely associated with the marked decrease of maternal passive immunity. The seropositive rate in winter (42.7%) was higher than in other seasons.
Parasites of feral pigeon (Columba livia) by fecal examination from some areas in Seoul, Korea
Kim, Se-Min ; Lee, Sang-Eun ; Kim, Neunghee ; Kim, Hera ; Kim, Youngseob ; Lee, Juhyoung ; Cho, Shin-Hyeong ; Lee, Won-Ja ; Park, Bae-Keun ; Jung, Bae-Dong ; Kim, Hyeon-Cheol ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 77~81
DOI : 10.7853/kjvs.2015.38.2.77
This study was conducted to evaluate the prevalence of helminths parasites from collected fecal samples of 410 feral pigeons (Columba livia) in 3 different areas of Gwangjin-gu, Seongdong-gu and Dongdaemun-gu in Seoul from December, 2011 to July, 2012 in Korea. The fecal samples were examined through by the centrifugation method using formalin-ether solution. The overall prevalence of parasites in the pigeons was 29.27% (120/410). Two nematodes (Tetrameres spp. and Capillaria spp.) and one protozoa (Eimeria spp.) were identified. In the case of nematodes, Tetrameres spp. dominated numerically (58, 14.15%), followed by Capillaria spp. (10, 2.44%) and oocysts of Eimeria spp. were detected in 78 cases (19.02%). This investigation introduces the result of examination on the prevalence of parasites in feral pigeons from some areas in Seoul for the first time.
Repellent effect of Eucalyptus detergent against house dust mites (Dermatophagoides farinae and Dermatophagoides pteronyssinus)
Kim, Hye-Sung ; Jee, Cha-Ho ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 83~87
DOI : 10.7853/kjvs.2015.38.2.83
The exposure to house dust mites is closely connected with allergic diseases such as atopic dermatitis, allergic rhinitis and asthma. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. Therefore, repelling the house dust mites is more effective method than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus oils are regarded as safe compounds, there have been attempts to commercialize and market the insecticides/repellent products containing Eucalyptus oil as such or based upon them. For that reason, we experimented repellent activity against these mites using laundry detergent with Eucalyptus oil in this experiment. House dust mites (Dermatophagoides farinae and Dermatophagoides pteronyssinus) were exposed at different concentrations (4, 8, 16,
) in fabric treated laundry detergent with Eucalyptus oil for 24 hours. The repellent effects (%) of laundry detergent with Eucalyptus oil exposed at 4, 8, 16, and
were 48.16%, 74.62%, 52.67%, and 44.47%, respectively. These results suggest that laundry detergent with Eucalyptus oil have potential effect to repel the house dust mites.
Survey on the gross lesions of slaughtered pigs in Jeonbuk area, Korea
Lim, Mi-Na ; Baek, Kui-Jeong ; You, Ki-Hong ; Cho, Hyun-Ung ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 89~94
DOI : 10.7853/kjvs.2015.38.2.89
Respiratory disease in pigs is common in modern pork production worldwide and is often referred to as porcine respiratory disease complex (PRDC). PRDC is polymicrobial in nature, and results from infection with various combinations of primary and secondary respiratory pathogens. The control of swine respiratory disease requires an understanding of the interactions between the organisms that can cause this illness, the pig and management of the environment. This study was carried out to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. A total of 900 lung samples, 45 farms were collected randomly from slaughtered pigs in Jeonbuk province from April to December in 2014. Gross lesions such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis, liver white spots were examined for the pigs. Overall prevalence of SEP was 70.8%. According to season, the incidence occurred higher in summer than winter, fall and spring. The mean SEP score was 1.4, the highest incidence occurred in fall. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 26.1%, 71.4%, 2.8%, 21.6%, respectively. In the detection of pathogens, PRRS was not detected, PCV2 was positive in 87.6%.
Prevalence and antimicrobial resistance of Salmonella isolated in poultry farms
Kang, Mi-Seon ; Lee, Su-Ji ; Shin, Young-Sik ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 95~100
DOI : 10.7853/kjvs.2015.38.2.95
An investigation was carried out to determine the prevalence and antimicrobial resistance of Salmonella isolated from chicks and ducks. A total of 2,522 samples collected from 281 farms were examined from 2013 to 2014. The overall prevalence of Salmonella spp. was 21.7% (61/281) of farms and 83 isolates (3.3%) were isolated from 2,522 samples. Nine serotypes of Salmonella spp. were identified such as S. Typhimurium (19/83), S. Enteritidis (12/83), S. London (11/83), S. Senftenberg (8/83), S. Infantis (4/83), S. Montevideo (3/83), S. Hadar (3/83), S. Saintpaul (1/83), S. Rissen (1/83) and S. Arizonae (2/83). Nineteen isolates were found to be untypable serotypes. In the results of antimicrobial resistance test, all of isolates were resistant to at least two antimicrobial agent and the high resistance was found to nalidixic acid (66.3%), streptomycin (57.8%). All of isolates were susceptible to amoxacillin/clavulic acid, cefeprime, ampicillin, sulfamethoxazole/trimethoprim. This results indicated the serotypes of Salmonella isolates are widely distributed in chicks and ducks. Therefore further epidemiological studies should be carried out in breeder farm and a hatchery.
A 16S rDNA polymerase chain reaction assay to detect Mycoplasma pulmonis in rats model
Hong, Sunhwa ; Lee, Hyun-A ; Choi, Yeon-Shik ; Chung, Yungho ; Kim, Okjin ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 101~106
DOI : 10.7853/kjvs.2015.38.2.101
Murine mycoplasmosis, caused by Mycoplasma (M.) pulmonis, is a prominent disease in rodent animals. The aim of this study was to develop a sensitive and specific PCR assay to detect M. pulmonis in animals and to assess the suitability of this assay for the detection of mycoplasmal infection in rats experimentally infected with M. pulmonis. A new PCR assay using the M. pulmonis-specific primer pairs MPul-F and MPul-R was developed. The primers and probe for the assay were designed from regions in the 16S rRNA gene that are unique to M. pulmonis. The novel PCR assay was very specific and sensitive for M. pulmonis, detecting the equivalent of 5 pg of target template DNA. It detected only M. pulmonis and no other Mycoplasma species or other bacterial species. The newly developed PCR assay also effectively detected M. pulmonis infection in rats. These results suggest that this PCR assay using M. pulmonis-specific primer pairs of MPul-F and MPul-R will be useful and effective for monitoring M. pulmonis infection in animals.
Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus
Kim, Eun-Mi ; Jeon, Hyo-Sung ; Kim, Ji Jung ; Kim, Hee-Jung ; Shin, Yeun-Kyung ; Song, Jae-Young ; Yeo, Sang-Geon ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 107~116
DOI : 10.7853/kjvs.2015.38.2.107
In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that accidentally infected in pig population. The RT-LAMP was completed in 40 min at
and the sensitivity of the RT-LAMP (
) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (
) and the same to real time RT-PCR (
). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diagnostic laboratory in the future.
Antioxidant activity and hepatic lipids improvement effects of Rubus coreanus in high-fat diet-fed rats
Lee, Soo-Jung ; Song, Yuno ; Chung, Min-Sup ; Jang, Sun-Hee ; Won, Chung-kil ; Song, Young-Min ; Cho, Jae-Hyeon ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 117~125
DOI : 10.7853/kjvs.2015.38.2.117
This study was investigated the effects on the radical scavenging activities and hepatic lipid levels in rats fed a high-fat diet (HFD) by the 70% ethanolic extract from Rubus coreanus. The Rubus coreanus extract (RCE) was administered through the gastrointestinal tract at 150 mg/kg BW/day for 5 weeks (HFD+RCE). Total phenol content was 157.93 mg gallic acid/g in the RCE. Scavenging activities to DPPH, ABTS, an d hydroxyl radicals by RCE significantly increased in a does-dependent manner of
concentration. DPPH and ABTS radical scavenging activities were over the 70% in the
concentration. During the five weeks study, the food efficiency ratio was no significant differences between the HFD and HFD+RCE. Liver weight of HFD+RCE (3.75 g/100 g BW) was significantly decreased compared to those of the HFD. Hepatic triglyceride and total cholesterol levels of HFD+RCE were significantly lower compared to those of the HFD. The hepatic TBARS contents in the HFD+RCE was significantly decreased to the HFD, radical scavenging activity was markedly increased in HFD+RCE as normal group. In histological observation of liver tissues, the hepatocytes of HFD+RCE appeared the reduced aspects on the size and numbers of lipid droplets in cytoplasm compared to the HFD. Our results suggest that RCE could have antioxidant activities and hepatic hypolipidemic effects by its phenolic compounds.
Simultaneous determination of preservatives in sausages using liquid chromatography with electrospray ionization tandem mass spectrometry
Koh, Ba-Ra-Da ; Kim, Ji-Yeon ; Jang, Mi-Sun ; Seo, Doo-Ri ; Jung, Bo-Ram ; Shin, Ji-Hyun ; Lim, Jin-Taek ; Kim, Yong-Hwan ; Kim, Eun-Sun ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 127~136
DOI : 10.7853/kjvs.2015.38.2.127
A simultaneous determination method was developed for nine preservatives (benzoic acid, sorbic acid, dehydroacetic acid, methyl-, ethyl-, isopropyl-, propyl-, isobutyl- and butyl-parabens) in sausage by liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Each parameter was established by multiple reaction monitoring in negative mode. Separation was achieved on a phenyl-hexyl (
, Waters) with A-20 mM ammonium acetate containing 0.1% acetic acid in water, B-Acetonitrile as mobile phase with gradient mode at a flow rate of 0.3 mL/min. The developed method was validated for specificity, linearity, accuracy and precision in sausages samples. Linearity was over 0.998 with calibration curve of the mixed standards. The mean recoveries from sausages fortified at the level of 2.0~10.0 mg/L were in range of 98.60~109.16% with RSDs lower than 8.93%. The limits of detection (LOD) and the limits of quantification (LOQ) were in the range between 0.0003~0.085 mg/L and 0.01~0.257 mg/L, respectively. Intra-day precision and inter-day precision were 0.45~6.16% and 2.81~13.33%, respectively. Using presently developed determination method, 33 field sausage samples from Gwangju city in Korea were screened over nine preservatives. As a result, no preservatives were detected in all samples.
Ear mite infestation in a lop-eared rabbit (Oryctolagus cuniculus) and successful treatment with ivermectin
Kim, Kyoo-Tae ; Lee, Seung-Hun ; Kwak, Dongmi ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 137~140
DOI : 10.7853/kjvs.2015.38.2.137
A 2-year-old female lop-eared rabbit (Oryctolagus cuniculus) was presented to the veterinary clinic at a zoo with pruritus, alopecia, and crusting of the ear. Examination of skin scrapes revealed an infestation with the rabbit ear mite, Psoroptes cuniculi. Weekly subcutaneous ivermectin injection over a three-week period resulted in remission of the clinical signs and improvement of the overall conditions of the rabbit.
The first virus isolation and partial characterization of equine herpesvirus-4 in a horse, South Korea
Choi, Eun-Jin ; Lee, Hyun-Kyoung ; Lee, Kyoung-Hyun ; So, Byoung-Jae ; Song, Jae-Young ; Do, Jae-Chul ; Yang, Seon-Joo ; Lee, Hyun-Chul ; Yang, Young-Jin ;
Korean Journal of Veterinary Service, volume 38, issue 2, 2015, Pages 141~144
DOI : 10.7853/kjvs.2015.38.2.141
An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author's knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.