Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Service
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Service
Editor in Chief :
Min-Hee Cho / Hee-Joung Song
Volume & Issues
Volume 38, Issue 4 - Dec 2015
Volume 38, Issue 3 - Sep 2015
Volume 38, Issue 2 - Jun 2015
Volume 38, Issue 1 - Mar 2015
Selecting the target year
Visual detection of porcine circovirus 2 by loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye
Kong, Ho-Chul ; Kim, Eun-Mi ; Jeon, Hyo-Sung ; Kim, Ji-Jung ; Kim, Hee-Jung ; Park, Yu-Ri ; Kang, Dae-Young ; Kim, Young-Hwa ; Park, Jun-Cheol ; Lee, Chang-hee ; Yeo, Sang-Geon ; Park, Choi-Kyu ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 145~153
DOI : 10.7853/kjvs.2015.38.3.145
In this study, we developed a loop-mediated isothermal amplification (LAMP) with hydroxynaphtol blue dye (HNB) for rapid and direct visual detection of porcine circovirus 2 DNA with high sensitivity and specificity. The LAMP was completed in 40 min at
, and the results of the LAMP can be confirmed by naked eye without any detection process. The sensitivity of the LAMP was 10-fold higher than that of the commercial PCR (cPCR) and real time PCR (rPCR) previously reported. In clinical application, the PCV2 detection rate of the LAMP was the same on porcine tissue samples (75.0%, 36/48) between porcine blood samples (75.0%, 39/52). The PCV2 detection rate (75.0%) of LAMP was higher than those of the cPCR and rPCR (67.3%, 35/52) in blood samples. In conclusion, the LAMP developed in the study could be an useful alternative method for the detection of PCV2 in the swine disease diagnostic laboratories.
Investigation of the incidence rate of second grade milk in dairy farms on the central-southern region of Korea
Jung, Ji-Young ; Yu, Do-Hyeon ; Shin, Sung-Shik ; Son, Chang-Ho ; Oh, Ki-Seok ; Hur, Tai-Young ; Jung, Young-Hun ; Choi, Chang-Yong ; Suh, Guk-Hyun ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 155~162
DOI : 10.7853/kjvs.2015.38.3.155
The incidence of second-grade milk production in 9 dairy farms of South Korea was investigated from May 2011 to March 2012, and the serum composition of cows producing first- and second-grade milk in 14 farms including the 9 farms was analyzed. The incidence rate of second-grade milk production of 402 cows in nine dairy farms located in the central and southwestern regions of Korea was 15.4% with the highest rate being 34.4%. Seasonal morbidity was higher during late winter (February) and early summer (June) with the highest rate observed in February (32.6%) followed by November (33.3%). Second-grade milk was most frequently found within one month postpartum (34.1%) while only 3.5% was found during the first 60~90 days of lactating period (n=785, 5 herds). The morbidity increased thereafter (P<0.05) with the highest observed between 270~300 days of lactation (36.1%). The acidity was not significantly different between second-grade (
) and first-grade milk (
). Blood serum analysis of 371 cows in the 14 dairy farms indicated that aspartate aminotransferase (AST) level was significantly higher (P<0.001) in cows producing second-grade milk while albumin was significantly lower (P<0.001) than cows producing first-grade milk. Total protein and triglyceride was also significantly low along with glucose, non-esterified fatty acid and blood urea nitrogen in cows producing second-grade milk. Statistical analysis including sensitivity, specificity and positive/negative prediction values showed that lactating cows with high AST, low albumin, total protein and triglyceride levels in the serum tended to produce second-grade milk. It was concluded that serological parameters, especially live functional and metabolic-related serum compositions (AST, albumin, total protein and triglyceride), were significantly influenced in cows producing second-grade milk.
Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) and porcine circovirus-2 (PCV-2) in pig farms in Gyeongbuk province
Sohn, Jun-Hyung ; Shin, Sung-Ho ; Kim, Soon-Tae ; Lee, Sung-sam ; Yun, Mun-Jo ; Cho, Gil-Jae ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 163~166
DOI : 10.7853/kjvs.2015.38.3.163
The purpose of this study was to survey seroprevalence of porcine reproductive and respiratory syndrome (PRRS) virus and porcine circovirus-2 (PCV-2) in Gyeongbuk province by enzyme-linked immunosorbent assay (ELISA). A total of 966 samples collected from 21 pig farms were tested. The sero-positive rate of PRRS and PCV-2 were 77.6% (750/966) and 76.4% (738/966), respectively.
Survey of pathogenic microorganisms contamination of chicken carcasses
Lee, Eun-Me ; Shin, Dong-Su ; Kwon, Mee-Soon ; Lee, Sung-Jae ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 167~171
DOI : 10.7853/kjvs.2015.38.3.167
Pathogenic microorganisms were monitored on the chicken carcasses in slaughterhouse of Jeonbuk area. It was conducted to evaluate the microbiological quality on 204 chicken carcasses. Staphylococcus (S.) aureus was isolated in largest number and its ration was 41.2%, Salmonella spp. 6.4%, Campylobacter (C.)jejuni 7.4%, C. coli 7.4%. Serotype of Salmonella (S.) spp. was identified as S. Infantis 46.1%, S. Enteritidis 23.1%, S. Typhimurium 7.7%, S. Montevideo 7.7%. In breed chickens, Salmonella spp. was detected broiler 4.1%, white semi-broiler 8.0% Korean native chicken 12.0%. C. jejuni was isolated broiler 7.4%, white semi-broiler 12.0%, Korean native chicken 0%, C. coli, broiler 7.4%, white semi-broiler 0%, Korean native chicken 18.1% and S. aureus, broiler 38.8%, white semi-broiler 40.0%, Korean native chicken 51.5%.
QuEChERS-based determination of tissue residues and acute toxicity of pyraclofos in rat
Pyo, Min-Jung ; Hah, Do-Yun ; Choi, You-Jeong ; Jeong, Kwi-Ok ; Han, Chang-Hee ; Park, Young-Ho ; Kim, Min-Hee ; Kim, Won-Gyu ; Jung, Jing-Gune ; Kim, Munki ; Kim, Euikyung ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 173~180
DOI : 10.7853/kjvs.2015.38.3.173
Environmental pesticides used for insect control can be transferred from plants to animals even to livestock animals through food chain. Human beings also can be exposed to pesticides by consuming polluted dairy products, including meats, eggs and other milk products. Therefore, the Ministry of Food and Drug Safety (MFDS) established Standard for Pesticide Residue Limits in dairy products. The QuEChERS (quick, easy, cheap, effective, rugged and safe) methods for detecting residual pesticides are relatively well established for fruits and vegetables, however, the methods for meat have not been appropriately studied yet. In the present work, pyraclofos was used as an organophosphate pesticide to examine its tissue residue in experimental animals by QuEChERS methods. For this, pyraclofos (150 mg/kg body weight) was orally administered to male rats once a day for 2 days. After 6, 12, and 24 hr of the treatment, the tissue residues in liver and femoral muscle of the rats were determined using QuEChERS methods followed by HPLC analyses. In preliminary studies, the recovery rates of spiking samples of pyraclofos demonstrated approximately 109~110% from the tissues. In previous study, pyraclofos tissue residues were observed with significantly high levels in livers and muscles at 6 hr of oral treatment. Then, they were almost completely disappeared after 24 hr of the administration, indicating the orally exposed pyraclofos is rapidly absorbed and distributed to body organs, then quickly excreted from the body with a negligible level of tissue residue. The alterations in blood chemistry as well as the histopathology of heart, lung, liver, spleen and kidney have also been investigated in the experimental animals for assessing acute toxic effects of pyraclofos. The obtained blood chemistry indexes (ALT and AST) showed maximum peak values at 12 hr after the oral administration and decreased to the normal levels at 24 hr of the treatment. Histopathologic observation exhibited acute hepatic damages at 24 hr of the treatment. In conclusion, we suggest that QuEChERS method can be adequately optimized for the analysis of pyraclofos residues in animal tissues.
The growth stage study on the femur and tibia of Korean native chicken after hatching
Tae, Hyun-Jin ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 181~188
DOI : 10.7853/kjvs.2015.38.3.181
Skeletal development of chicken has been widely discussed in industrial forums and various research reports. However, these studies were emphasis on the commercial chicken strains for improve egg and meat production whereas the skeletal quiet remains as a potential weak link related to facilitating in the physical support of heavier carcasses at ever younger ages. For that, the study of standardization of skeletal development is important but it was rarely reported in Korean native chicken (KNC). The study was investigated the skeletal characteristics of KNC for international standardization. We studied in KNC at 2, 14, 28, 42, 56, 70, 84, 98, 112, 126, 147, 168, 196, 224, 336 and 448 days after hatch (male and female, n=13 for each group). We measured the body weight (BW), and after sacrifice measured organs and remove muscle from femur & tibia and measured bone weight. Data were analyzed by ANOVA, Duncan test, correlation analysis and regression analysis of SAS 9.1. We analyzed the data of BW, femur & tibia and made growth curve also. The BW was significantly increased up to 147 days after hatch (male,
), and then increased gradually. At 336 days, these growth was stop (male,
). The growth of femur & tibia length and width was stop on 98~126 days after hatch. At 98 days, we measured the length and width of femur & tibia in male were
whereas in female at 112 days the length of femur & tibia was
and at 126 days width was
. Our study suggests that the growth of male KNC was classified pre-puberty (0~98 days), puberty (98~336 days) and maturity (after 336 days), meanwhile female was shown similar trend however puberty period of KNC was 112 or 126 days after hatch.
Epitheliogenesis imperfecta in three Korean indigenous calves
Lim, Ji-Hee ; Lee, Hyun-kyoung ; Lee, Kyung-hyun ; Baek, Kang-hyun ; Lee, Myoung-Heon ; Choi, Eun-Jin ; Bae, You-Chan ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 189~193
DOI : 10.7853/kjvs.2015.38.3.189
Epitheliogenesis imperfecta is a congenital disease in which localized or widespread defect areas of squamous epithelium of the skin. Epitheliogenesis imperfecta is rare anomaly of calves, piglets, foals, lambs, puppies, and kittens. Three cases of epitheliogenesis imperfecta in Korean indigenous calves were diagnosed in animal and Plant Quarantine Agency in 2011~2014. In this paper, we tried to report them in detailed and compare with similar cases occurred in other countries.
Mannheimiosis case in Hanwoo caused by heat stress
Lee, Jeong-Won ; Shon, Ku-Rye ; Jeong, Han-Sol ; Ko, Won-Seok ; Lim, Chae-Woong ; Kim, Bum-Seok ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 195~198
DOI : 10.7853/kjvs.2015.38.3.195
Mannheimia haemolytica is an opportunistic bacterium that is widely recognized among the bovine respiratory disease (BRD) complex in cattle. Five Hanwoo with a history of fever, anorexia and dyspnea were died within 2 days in a the middle of summer. Four cattle were pregnant. The cattle house were located in mountainous area but the window for air ventilation was open only one side. In addition, the fecal material for fermentation was located indoor. Air ceiling fan did not work. The indoor temperature was
. After working on air fan, the indoor temperature was still
. On necropsy, there was fibrinous pleuritis with a rich yellowish pleural fluids in the thorax. The cross-section of the lung showed lobar fibrinonecrotic pneumonia with expanded interlobular septa by edema and fibrin. Microscopically, parenchymal necrosis with dense layer of inflammatory cells were observed surrounding interlobular septum. Fibrin and inflammatory cells were filled in the alveoli. Bacteriological cultures of pulmonary tissue showed growth of M. haemolytica. This pneumonia case in Hanwoo suggests that environmental stressors such as high temperature, insufficient air ventilation, and pregnancy be the cause of mannheimoisis. Control of environmental stressor, such as temperature indoor is necessary to prevent BRD caused by M. haemolytica.
Malignant mesothelioma of omentum in a dog
Park, Ja-Sil ; Min, Chang-Woo ; Kim, Ah-Young ; Lee, Eun-Mi ; Lee, Eun-Joo ; Kang, Kyung-Ku ; Kim, Sang-Hyeob ; Lee, Myeong-Mi ; Sung, Soo-Eun ; Hwang, Meeyul ; Kwon, Young-Sam ; Kim, Tae-Hwan ; Kim, Jae-Hoon ; Jeong, Kyu-Shik ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 199~203
DOI : 10.7853/kjvs.2015.38.3.199
An 8-year old intact female poodle was presented to clinics due to abdominal distension, anorexia, and labored breath associated with pleural effusion. Intra-operative findings revealed multiple neoplasm of the greater omentum, involving anterolateral abdominal wall, sterna surface in the pleural cavity and diaphragm. These masses were 0.1~0.5 cm in diameter and extended to ovaries, pancreas, and serosal surface of stomach. Microscopically, most neoplastic cells had oval nuclei with prominent nucleoli and abundant eosinophilic cytoplasm. In deeper area, neoplastic acinus or glandular structures showed invaginated growth resembling adenocarcinoma. High mitotic figures were observed. By immunohistochemistry, the neoplastic cells were strong positive both cytokeratin and vimentin. The present case described for malignant mesothelioma in a dog. Our findings might be helpful for diagnosis and information and helped the clinics choose the treatment including chemotherapy such as cisplatin.
Gastrointestinal lymphoma in a Ferret
Lee, Su-Hyang ; Kim, Sang-Hyeob ; Lee, Eun-Joo ; Park, Soon-Seok ; Kim, Ah-Young ; Lee, Eun-Mi ; Lee, Myeong-Mi ; Sung, Soo-Eun ; Hwang, Meeyul ; Kwon, Young-Sam ; Kim, Tae-Hwan ; Jeong, Kyu-Shik ;
Korean Journal of Veterinary Service, volume 38, issue 3, 2015, Pages 205~209
DOI : 10.7853/kjvs.2015.38.3.205
A 7 years old, female and neutered ferret (Mustela putorius furo) was presented to local animal hospital due to lethargy, anorexia and hypothermia. Radiography, ultrasonography and blood test were performed. On the basis of clinical signs and tests, this case was presumed to be lymphoma. In consideration of this ferret's condition, chemotherapy was carried out for the treatment. However, the ferret died a few days. After necropsy, metastatic tumors were observed over abdomen. All tumors were packed with homologous lymphocytes with pleomorphic changes and numerous mitotic figures. Consequently, this masses were diagnosed as B-cell gastrointestinal lymphoma, which were negative for CD3 on immunohistochemistry.